LOCUS       sequence-20979-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GAL-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(25..149)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(157..173)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(157..173)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(188..629)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(194..217)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    512..629
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(653..673)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(653..671)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cctaggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
       61 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      121 tctcgttcag cttttttgta caaacttgtg atgggggatc cactagttct agaatccggg
      181 gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt
      241 attacatttg aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt
      301 tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta
      361 attaccccag aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg
      421 attcgttcat ttgaaggttt gtggggccag gttactgcca atttttcctc ttcataacca
      481 taaaagctag tattgtagaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc
      541 gtttcaggaa cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg
      601 ctgtcacccg ctcggcggct tctaatccgt actagagctc cagcttttgt tccctttagt
      661 gagggttaat tgcgcgcttg gcgtaatcat ggtcataact gtttcctgtg
//
