LOCUS       sequence-209791-       12781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Tuttle JR, Haigler CH, Robertson D
  TITLE     Method: low-cost delivery of the cotton leaf crumple virus-induced 
            gene silencing system.
  JOURNAL   Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.
  PUBMED    22853641
REFERENCE   2  (bases 1 to 12781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2012-08-1"; volume: "8(1):27. doi"; pages: " 
            10.1186/1746-4811-8-27"
COMMENT     Sequence Label: pJRT.Agro.CLCrVA.008
FEATURES             Location/Qualifiers
     source          1..12781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2545..2562)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2545..2561)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2554..2576)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2764..2788
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4089..4718
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5147..6220
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6286..6480
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6716..6738)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6824..6964
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6879..6898
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6979..6996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7150..7738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7230..7249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9163..9957)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10382..10406
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10484..10658
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10699..11724)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11791..12467)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(11834..11854)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    12658..12679
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12694..12699,12700..12717,12718..12724)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12732..12748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12737..12759
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12756..12772
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12756..12772
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct ccacttggga taggttaaga aaatatgtct ggcttgtaaa cgaaaagaat
       61 cggggtttct aggcattttt taaatagtga cccaggactc aaaaactagc tctcaacttc
      121 tctcatatgt tgtggagtct ggagtccttt atatatgttt acgctataga aggcatttgg
      181 ggactctccc gggcccacca taggactcca taccgcggcc atctatataa tattactgat
      241 ggccgcgcgg attttggagc ccccttttgt cgttagcgcg acctttaatt caaattaaag
      301 gttgcttttt tctatcgtcc gatcattttg cgcctgacta gcctagatat tagcaacaac
      361 ttcgcggcta agttgttgac cgtcaagtat aaattaatgg gcttatggcc taatgggtat
      421 agagcaaaat ggcatgcctg cagactagtg aattcgctag cgttaactgg ccataatcct
      481 gtgtatgcaa cgttgaaaat tcggatctat ttttacgatt cgatcatgaa ttaataaaat
      541 ttaaatttta ttgaatgatt ttctagtaca taactgacat acgacttatc agttgcgaaa
      601 cgaacagctc taattacatt gttaagtgaa attacgccta attcgtctaa gtacaacata
      661 accaactgtt taaacctaat taaataagtc gacccagaag ctgtcagaga tgtcgtccag
      721 acttggaagt tcaggtaagc tttgtggaga tgcaacgctc tccgtaagtt gtggttgaat
      781 cggatctgga cgtggtatat acgcgtcctg gtgtatagca gatcctctac tccgtatatc
      841 ttgaaatata ggggatttat tatctcccag atatacacgc cattctctgc ctgacgtgca
      901 gtgatgattt cccctgtgcg tgaatccatg accggtgcag cctatgtgga agtatatggt
      961 gcacccacag tttagatcaa tgcgtcgtcg tctaatcgcc ctcctcttgg cttgcctgtg
     1021 tgccttcttg atagaggggg gcgtcgagag tgatgaagat aacattcttg agagtccaat
     1081 gtttgagcga cgcattttcc gctttatcca ggaaatcttt atagctggat ccctcgcctg
     1141 gattgcaaag cacgattgaa gggatccctc ctttaatttg aactggtttc ccgtacttgc
     1201 aatttgactg ccagtctttt tgggccccaa gaagctcttt ccaatgcttt agctttaggt
     1261 attgcggtgc tacgtcatca atgacgttat aggcaacatt attcgagtac actcgattat
     1321 tgaagtccag gtgtccactg aggtaattat gtgggcctaa agcacgagcc cacatcgtct
     1381 tgcccgttct agaatcacct tccactatga gactaactgg tcttacaggc cgcgcagcgg
     1441 gacccattcc aaaataatca tccgcccatt cttgcatctg ttcaggaacg ttagtgaaag
     1501 aggaaagtgg aaatggaggg acccattgtt caggcgcctt tttgaaaagg cgttccaagt
     1561 tcgctgtaat gttatggtaa ctcacgatga acgcccttgg atctccggcc cggataattg
     1621 caagagcttc cgccaaacta ggtgcattga tggcgttata atagacgtcg tctttattct
     1681 tctttgtacc cccaggcact ttgaactgtc cggattcaca acaatcgccg tctttggtga
     1741 tgtaattctt gacggcgttg gcgtctttgg ctgcttgtat gtttgggtga aaattggtcg
     1801 accttctggg gtgagtaagg tcgaaaaatc tcttattctt gatgttggac tttccagaga
     1861 gttggatgag acagtgaagg tgtgggaatc cgtccgagtg ctcctctctg gcaactcgaa
     1921 tatatgttgg tttgacgact gaccatgata aggtttgaag catttgaaga gcttcatttt
     1981 ttgggatatc gcacttggga taggttaaga aaatatgtct ggcttgtaaa cgaaaagaat
     2041 cggggtttct aggcattttt taaatagtga cccaggactc aaaaactagc tctcaacttc
     2101 tctcatatgt tgtggagtct ggagtccttt atatatgttt acgctataga aggcatttgg
     2161 ggactctccc gggcccacca taggactcca taccgcggcc atctatataa tattactgat
     2221 ggccgcgcgg attttggagc ccccttttgt cgttagcgcg acctttaatt caaattaaag
     2281 gttgcttttt tctatcgtcc aatcattttg cgcctgacta gcctagatat tagcaacaac
     2341 ttcgcgacta agttgttgac cgtcaagtat aaattaatgg gcttatggcc caatgggtat
     2401 agagcaaaat gcccaagcgt gatgccccgt ggcgcttatt agcgggtacg tctaaggtaa
     2461 gtcgaaatgc caactactct cccttgagag tattgggccc taaagttaat aagggacctc
     2521 gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2581 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2641 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2701 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
     2761 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     2821 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     2881 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     2941 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     3001 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3061 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3121 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3181 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3241 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     3301 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     3361 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     3421 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     3481 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     3541 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     3601 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     3661 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     3721 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     3781 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     3841 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     3901 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     3961 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     4021 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     4081 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     4141 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     4201 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     4261 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     4321 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     4381 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     4441 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     4501 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     4561 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     4621 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     4681 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     4741 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     4801 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     4861 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     4921 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     4981 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     5041 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     5101 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     5161 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     5221 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     5281 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     5341 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     5401 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     5461 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     5521 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     5581 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     5641 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     5701 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     5761 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     5821 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     5881 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     5941 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     6001 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     6061 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     6121 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     6181 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     6241 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
     6301 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     6361 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     6421 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     6481 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     6541 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     6601 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     6661 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     6721 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     6781 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     6841 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     6901 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     6961 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7081 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7321 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7441 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7501 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7561 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     7621 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7681 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7741 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7801 gctgatgaat cccctaatga ttttggtaaa aatcattaag ttaaggtgga tacacatctt
     7861 gtcatatgat caaatggttt cgcgaaaaat caataatcag acaacaagat gtgcgaactc
     7921 gatattttac acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac
     7981 agcttaacgt tggcttgcca cgcattactt gactgtaaaa ctctcactct taccgaactt
     8041 ggccgtaacc tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg
     8101 ttaggtaatc gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt
     8161 atctgttcgg gcaatacgat gcccattgta cttgttgact ggtctgatat tcgtgagcaa
     8221 aaacgactta tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat
     8281 gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc
     8341 gaccttgcga gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt
     8401 aaagtgccat ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga
     8461 ggaaaagtac aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat
     8521 gatatgtcat ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca
     8581 atctcatgcc aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg
     8641 acacggactc attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca
     8701 tgggttctag caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc
     8761 tattcgaagc gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga
     8821 ctaggcctac gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc
     8881 gccctgatgc ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg
     8941 gacaagcact tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta
     9001 ggcatggaag ttttgcggca ttctggctac acaataacaa gggaagactt actcgtggct
     9061 gcaaccctac tagctcaaaa tttattcaca catggttacg ctttggggaa attatgaggg
     9121 gatctctcag cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
     9181 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     9241 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     9301 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
     9361 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
     9421 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
     9481 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
     9541 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
     9601 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
     9661 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
     9721 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
     9781 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
     9841 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
     9901 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
     9961 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    10021 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    10081 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    10141 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    10201 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    10261 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    10321 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    10381 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
    10441 ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac
    10501 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
    10561 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc
    10621 ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga
    10681 tcccggtcgg catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc
    10741 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
    10801 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
    10861 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
    10921 gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa
    10981 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
    11041 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
    11101 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
    11161 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
    11221 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
    11281 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
    11341 gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg
    11401 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg agatgcaata
    11461 ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
    11521 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
    11581 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
    11641 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
    11701 cgcggtgagt tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag
    11761 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
    11821 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
    11881 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
    11941 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
    12001 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
    12061 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
    12121 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
    12181 cttggagtag acgagagtgt cgtgctccac catgttcaca tcaatccact tgctttgaag
    12241 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
    12301 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
    12361 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
    12421 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
    12481 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
    12541 tggcaagctg ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    12601 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    12661 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    12721 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
    12781 g
//
