LOCUS       sequence-209799-        8299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pScaf phagemid with insert for generating DNA origami scaffold 5544 
            bases in length (v4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds.
  JOURNAL   Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 
            2018 Aug 9.
  PUBMED    30984875
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2018-01"; volume: "3(1). doi"; pages: " 
            10.1093/synbio/ysy015. Epub 2018 Aug 9"
COMMENT     Sequence Label: pScaf-5544.4
FEATURES             Location/Qualifiers
     source          1..8299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      44..424
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5933..5949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5933..5949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5946..5968)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5957..5973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5981..5987,5988..6005,6006..6011))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6026..6047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6164..6181)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6335..6923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6415..6434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7094..7885,7886..7954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7955..8059)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8127..8145
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8183..8205)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc ttttttagat ctagcctgca gtaatagagc ggcccaggat gcggagttgg
      481 gaaatcacct gagatgagat catgcgctgt gatgagcgtg caatgttggc aggcagaccg
      541 aagaaggatg gcttatcgtc ttctggaatc ttctcaatga cagccctgta atccagaatg
      601 ctgcagcttt gtggcaggct cacgctgtag ggaaagatgg acttcttgtt gcgctgattg
      661 aacacgtcaa tgacgctaga gttgaagaat tgcttcaaat agctctgcag gacgcgcaag
      721 tcgaaatagt tatcgatgcg gcctccgtag atggcgttct ccagcagacc atggacaaac
      781 tcccattgca cgtccttggc accatcaaag agcctgtcaa tgatattgta accggccctc
      841 aggtcggaga gagagaactc gtagaatttg gtccaacctt gaggaatgta gttgcgcctc
      901 tcttggcatg cggcgtgaaa ccaggccaga gagaacagag catgtgcgcg gtgagtgtta
      961 tctttcttag agatttgttc aggtgtccaa gattcatatg tgcgcatgag attcttcttg
     1021 agtccaggtg gtgattcata agtgatcttg aggcttgact ggaggaggat aggtgtaaag
     1081 tttgggtgca cttcggctgt caaccagagc ctgaaagtgt ctttgggttg caaggtgttc
     1141 agttccttct ccaagacagg cagccaggag acaaccaggt gcaagttctt caaacacagc
     1201 cagtcaccat tccttgcgca ttccttgagc atttggatag ccaagtcagc ttggccctgg
     1261 cccattgcaa cctggtggta acattctcct gatctttcgg cgttggcgag ctcctggagt
     1321 tcctgggagg gatcggctcc tggagaaata atgatcagaa tgggctcgat ttcgagtgtc
     1381 tccttgtaca gcctcttgag attgagtggg agtggagaga cttccttcaa acccaaggtc
     1441 ttgcaggcaa agagggccat agctgactgc aatctatctg gcctgagcac ctgcacgacg
     1501 aggatctgct ggaacaagct gaccttctta gccaagatgc taggaaattc ctgctcacac
     1561 atgctgttgt tgtagtaggt gcgccacaaa gcggcgtcct cgaaacacag agtctggtac
     1621 agggaaggca gagcgatctt caaggttgcc acagcccagg atctctcttg atcgatccat
     1681 gaaggcaact ggtccctgat cttctgctgg gagtcggctt tgcgcaacat gtctccgacc
     1741 accacgccag tgaaggtatc ccattcgttc tcctggaaca attctggatg catacctctg
     1801 acgaaatgca gggcgaacat cagctggtcg gccttgaaca ggcagcggca gatgtattcg
     1861 tacaccatgt gctgcaggga gctgatcagg gattgaatcc tctgttctgt gttctcagaa
     1921 tcctgcttat tctggagtgc cctttgaaac aacctcagaa aggcggccag ggagaacctg
     1981 tacatattat tgatcttgga gaggtcagag ataatgaagt acatctttga agcagactca
     2041 gcgagtggca gatatgcgtc gcgctcctgg tcgagagaga tttgcagctt ataagattcc
     2101 ttcagagact cttggatgag ggcggaagat gctttagtct gattcagaga ctcgatcagg
     2161 tccttgttct ccaggatgtt gccctgagaa gtagccaatg tctccagcag agattcttcg
     2221 agtttggcca gttggatctt cttatcctcc tcctgttgga gcagcttagt cttctgttct
     2281 tccaaatcgg gtttctcatg ttggatggtg agggccagga gctggcctct cagtcctgag
     2341 cgggtggttg tgaaattaac ctctgtgacg atgctggcag cgtctggtgg aatgaatggg
     2401 ttgggattcc tagtgctcag gaacaggcga aactcctcgt tatagtcgat gatcttatct
     2461 ccaatctgga ccacatagcg tgggccttgg gccaccaaat ctctgcgcaa cagagggtac
     2521 agaactggct ccacgccatc catctcctgg atgatcaagg tcttgccgaa gcggacggcg
     2581 agttccaggg cagtgatgaa gttggagtct tgctggttga tcacttccag cctggaatcc
     2641 ttcaagtgag tcttcagcca ctcagtagcc tgtgagctgg gatcgatgag gaaagggcaa
     2701 acgcgggatt gcaagataac cagtgcgttc tcgattgaga ggtcatcgct gggcagacct
     2761 tcagacttcc agatcagctg ctctgactcg gtgcagagaa atcttctcag gtcaaacttc
     2821 tccaggccgg cagacttggt ccactcctcc agacatgtct ttctgagaga ctcaggtgca
     2881 gcggacaagt aggtgataaa ggctgcggcc aactgagccc tctttgggag ggttgccagc
     2941 tcctcagtaa tctcgacgac ctgagcattc catctcttgt gttcgcggtc cggatcttcc
     3001 ggtgatgagc gatatcccgg cactgtcaga tttgatcacc agtatggtgg ccagcggcta
     3061 tgactaccgg cgcgacgatg atgcgggctt gtggagttca gccgatctga cttatgtcat
     3121 tacctatgaa atgtgaggac gctatgcctg taccaaatcc tacaatgccg gtgaaaggtg
     3181 ccgggaccac cctgtgggtt tataagggga gcggtgaccc ttacgcgaat ccgctttcag
     3241 acgttgactg gtcgcgtctg gcaaaagtta aagacctgac gcccggcgaa ctgaccgctg
     3301 agtcctatga cgacagctat ctcgatgatg aagatgcaga ctggactgcg accgggcagg
     3361 ggcagaaatc tgccggagat accagcttca cgctggcgtg gatgcccgga gagcaggggc
     3421 agcaggcgct gctggcgtgg tttaatgaag gcgatacccg tgcctataaa atccgcttcc
     3481 cgaacggcac ggtcgatgtg ttccgtggct gggtcagcag tatcggtaag gcggtgacgg
     3541 cgaaggaagt gatcacccgc acggtgaaag tcaccaatgt gggacgtccg tcgatggcag
     3601 aagatcgcag cacggtaaca gcggcaaccg gcatgaccgt gacgcctgcc agcacctcgg
     3661 tggtgaaagg gcagagcacc acgctgaccg tggccttcca gccggagggc gtaaccgaca
     3721 agagctttcg tgcggtgtct gcggataaaa caaaagccac cgtgtcggtc agtggtatga
     3781 ccatcaccgt gaacggcgtt gctgcaggca aggtcaacat tccggttgta tccggtaatg
     3841 gtgagtttgc tgcggttgca gaaattaccg tcaccgccag ttaatccgga gagtcagcga
     3901 tgttcctgaa aaccgaatca tttgaacata acggtgtgac cgtcacgctt tctgaactgt
     3961 cagccctgca gcgcattgag catctcgccc tgatgaaacg gcaggcagaa caggcggagt
     4021 cagacagcaa ccggaagttt actgtggaag acgccatcag aaccggcgcg tttctggtgg
     4081 cgatgtccct gtggcataac catccgcaga agacgcagat gccgtccatg aatgaagccg
     4141 ttaaacagat tgagcaggaa gtgcttacca cctggcccac ggaggcaatt tctcatgctg
     4201 aaaacgtggt gtaccggctg tctggtatgt atgagtttgt ggtgaataat gcccctgaac
     4261 agacagagga cgccgggccc gcagagcctg tttctgcggg aaagtgttcg acggtgagct
     4321 gagttttgcc ctgaaactgg cgcgtgagat ggggcgaccc gactggcgtg ccatgcttgc
     4381 cgggatgtca tccacggagt atgccgactg gcaccgcttt tacagtaccc attattttca
     4441 tgatgttctg ctggatatgc acttttccgg gctgacgtac accgtgctca gcctgttttt
     4501 cagcgatccg gatatgcatc cgctggattt cagtctgctg aaccggcgcg aggctgacga
     4561 agagcctgaa gatgatgtgc tgatgcagaa agcggcaggg cttgccggag gtgtccgctt
     4621 tggcccggac gggaatgaag ttatccccgc ttccccggat gtggcggaca tgacggagga
     4681 tgacgtaatg ctgatgacag tatcagaagg gatcgcagga ggagtccggt atggctgaac
     4741 cggtaggcga tctggtcgtt gatttgagtc tggatgcggc cagatttgac gagcagatgg
     4801 ccagagtcag gcgtcatttt tctggtacgg aaagtgatgc gaaaaaaaca gcggcagtcg
     4861 ttgaacagtc gctgagccga caggcgctgg ctgcacagaa agcggggatt tccgtcgggc
     4921 agtataaagc cgccatgcgt atgctgcctg cacagttcac cgacgtggcc acgcagcttg
     4981 caggcgggca aagtccgtgg ctgatcctgc tgcaacaggg ggggcaggtg aaggactcct
     5041 tcggcgggat gatccccatg ttcagggggc ttgccggtgc gatcaccctg ccgatggtgg
     5101 gggccacctc gctggcggtg gcgaccggtg cgctggcgta tgcctggtat cagggcaact
     5161 caaccctgtc cgatttcaac aaaacgctgg tcctttccgg caatcaggcg ggactgacgg
     5221 cagatcgtat gctggtcctg tccagagccg ggcaggcggc agggctgacg tttaaccaga
     5281 ccagcgagtc actcagcgca ctggttaagg cgggggtaag cggtgaggct cagattgcgt
     5341 ccatcagcca gagtgtggcg cgtttctcct ctgcatccgg cgtggaggtg gacaaggtcg
     5401 ctgaagcctt cgggaagctg accacagacc cgacgtcggg gctgacggcg atggctcgcc
     5461 agttccataa cgtgtcggcg gagcagattg cgtatgttgc tcagttgcag cgttccggcg
     5521 atgaagccgg ggcattgcag gcggcgaacg aggccgcaac gaaagggttt gatgaccaga
     5581 cggatccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5761 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     5821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5881 aatagtggac tcttgttcca aactggttta acactcagaa ttcgtaatca tggtcatagc
     5941 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6001 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6061 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6121 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6181 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6241 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6301 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6361 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6421 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6481 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6541 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6601 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6661 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6721 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     6781 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6841 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6901 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6961 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7021 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7081 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7141 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7201 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7261 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7321 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7381 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7441 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7501 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7561 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7621 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7681 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7741 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7801 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7861 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7921 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7981 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8041 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     8101 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     8161 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     8221 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     8281 ggtgtcgggg ctggcttaa
//
