LOCUS       sequence-209803-       10819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pScaf phagemid with insert for generating DNA origami scaffold 8064 
            bases in length (v2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds.
  JOURNAL   Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 
            2018 Aug 9.
  PUBMED    30984875
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2018-01"; volume: "3(1). doi"; pages: " 
            10.1093/synbio/ysy015. Epub 2018 Aug 9"
COMMENT     Sequence Label: pScaf-8064.2
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      44..424
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     protein_bind    2606..2837
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     primer_bind     complement(8453..8469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8453..8469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8466..8488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8477..8493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8501..8507,8508..8525,8526..8531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8546..8567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8684..8701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8855..9443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8935..8954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9614..10405,10406..10474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10237..10256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10475..10579)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10647..10665
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10703..10725)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc ttttttagat ctcattatca tcgcttttaa aacggttcga ccttctaatc
      481 ctatctgacc attataattt tttagaatgg tttcataaga aagctctgaa tcaacggact
      541 gcgataataa gtggtggtat ccagaatttg tcacttcaag taaaaacacc tcacgagtta
      601 aaacacctaa gttctcaccg aatgtctcaa tatccggacg gataatattt attgcttctc
      661 ttgaccgtag gactttccac atgcaggatt ttggaacctc ttgcagtact actggggaat
      721 gagttgcaat tattgctaca ccattgcgtg catcgagtaa gtcgcttaat gttcgtaaaa
      781 aagcagagag caaaggtgga tgcagatgaa cctctggttc atcgaataaa actaatgact
      841 tttcgccaac gacatctact aatcttgtga tagtaaataa aacaattgca tgtccagagc
      901 tcattcgaag cagatatttc tggatattgt cataaaacaa tttagtgaat ttatcatcgt
      961 ccacttgaat ctgtggttca ttacgtctta actcttcata tttagaaatg aggctgatga
     1021 gttccatatt tgaaaagttt tcatcactac ttagtttttt gatagcttca agccagagtt
     1081 gtctttttct atctactctc atacaaccaa taaatgctga aatgaattct aagcggagat
     1141 cgcctagtga ttttaaacta ttgctggcag cattcttgag tccaatataa aagtattgtg
     1201 taccttttgc tgggtcaggt tgttctttag gaggagtaaa aggatcaaat gcactaaacg
     1261 aaactgaaac aagcgatcga aaatatccct ttgggattct tgactcgata agtctattat
     1321 tttcagagaa aaaatattca ttgttttctg ggttggtgat tgcaccaatc attccattca
     1381 aaattgttgt tttaccacac ccattccgcc cgataaaagc atgaatgttc gtgctgggca
     1441 tagaattaac cgtcacctca aaaggtatag ttaaatcact gaatccggga gcactttttc
     1501 tattaaatga aaagtggaaa tctgacaatt ctggcaaacc atttaacaca cgtgcgaact
     1561 gtccatgaat ttctgaaaga gttacccctc taagtaatga ggtgttaagg acgctttcat
     1621 tttcaatgtc ggctaatcga tttggccata ctactaaatc ctgaatagct ttaagaaggt
     1681 tatgtttaaa accatcgctt aatttgctga gattaacata gtagtcaatg ctttcaccta
     1741 aggaaaaaaa catttcaggg agttgactga attttttatc tattaatgaa taagtgctta
     1801 cttcttcttt ttgacctaca aaaccaattt taacatttcc gatatcgcat ttttcaccat
     1861 gctcatcaaa gacagtaaga taaaacattg taacaaagga atagtcattc caaccatctg
     1921 ctcgtaggaa tgccttattt ttttctactg caggaatata cccgcctctt tcaataacac
     1981 taaactccaa catatagtaa cccttaattt tattaaaata accgcaattt atttggcggc
     2041 aacacaggat ctctctttta agttactctc tattacatac gttttccatc taaaaattag
     2101 tagtattgaa cttaacgggg catcgtattg tagttttcca tatttagctt tctgcttcct
     2161 tttggataac ccactgttat tcatgttgca tggtgcactg tttataccaa cgatatagtc
     2221 tattaatgca tatatagtat cgccgaacga ttagctcttc aggcttctga agaagcgttt
     2281 caagtactaa taagccgata gatagccacg gacttcgtag ccatttttca taagtgttaa
     2341 cttccgctcc tcgctcataa cagacattca ctacagttat ggcggaaagg tatgcatgct
     2401 gggtgtgggg aagtcgtgaa agaaaagaag tcagctgcgt cgtttgacat cactgctatc
     2461 ttcttactgg ttatgcaggt cgtagtgggt ggcacacaaa gctttgcact ggattgcgag
     2521 gctttgtgct tctctggagt gcgacaggtt tgatgacaaa aaattagcgc aagaagacaa
     2581 aaatcacctt gcgctaatgc tctgttacag gtcactaata ccatctaagt agttgattca
     2641 tagtgactgc atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     2701 atttaatata ttgatattta tatcatttta cgtttctcgt tcagcttttt tatactaagt
     2761 tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2821 aaataaaatc attatttgat ttcaattttg tcccactccc tgcctctgtc atcacgatac
     2881 tgtgatgcca tggtgtccga cttatgcccg agaagatgtt gagcaaactt atcgcttatc
     2941 tgcttctcat agagtcttgc agacaaactg cgcaactcgt gaaaggtagg cggatcttct
     3001 tgaccagcca gaaaacgacc tttctgtggt gaaaccggat gctgcaattc agagcggcag
     3061 caagtggggg acagcagaag acctgaccgc cgcagagtgg atgtttgaca tggtgaagac
     3121 tatcgcacca tcagccagaa aaccgaattt tgctgggtgg gctaacgata tccgcctgat
     3181 gcgtgaacgt gacggacgta accaccgcga catgtgtgtg ctgttccgct gggcatgcca
     3241 ggacaacttc tggtccggta acgtgctgag cccggccaaa ctccgcgata agtggaccca
     3301 actcgaaatc aaccgtaaca agcaacaggc aggcgtgaca gccagcaaac caaaactcga
     3361 cctgacaaac acagactgga tttacggggt ggatctatga aaaacatcgc cgcacagatg
     3421 gttaactttg accgtgagca gatgcgtcgg atcgccaaca acatgccgga acagtacgac
     3481 gaaaagccgc aggtacagca ggtagcgcag atcatcaacg gtgtgttcag ccagttactg
     3541 gcaactttcc cggcgagcct ggctaaccgt gaccagaacg aagtgaacga aatccgtcgc
     3601 cagtgggttc tggcttttcg ggaaaacggg atcaccacga tggaacaggt taacgcagga
     3661 atgcgcgtag cccgtcggca gaatcgacca tttctgccat cacccgggca gtttgttgca
     3721 tggtgccggg aagaagcatc cgttaccgcc ggactgccaa acgtcagcga gctggttgat
     3781 atggtttacg agtattgccg gaagcgaggc ctgtatccgg atgcggagtc ttatccgtgg
     3841 aaatcaaacg cgcactactg gctggttacc aacctgtatc agaacatgcg ggccaatgcg
     3901 cttactgatg cggaattacg ccgtaaggcc gcagatgagc ttgtccatat gactgcgaga
     3961 attaaccgtg gtgaggcgat ccctgaacca gtaaaacaac ttcctgtcat gggcggtaga
     4021 cctctaaatc gtgcacaggc tctggcgaag atcgcagaaa tcaaagctaa gttcggactg
     4081 aaaggagcaa gtgtatgacg ggcaaagagg caattattca ttacctgggg acgcataata
     4141 gcttctgtgc gccggacgtt gccgcgctaa caggcgcaac agtaaccagc ataaatcagg
     4201 ccgcggctaa aatggcacgg gcaggtcttc tggttatcga aggtaaggtc tggcgaacgg
     4261 tgtattaccg gtttgctacc agggaagaac gggaaggaaa gatgagcacg aacctggttt
     4321 ttaaggagtg tcgccagagt gccgcgatga aacgggtatt ggcggtatat ggagttaaaa
     4381 gatgaccatc tacattactg agctaataac aggcctgctg gtaatcgcag gcctttttat
     4441 ttgggggaga gggaagtcat gaaaaaacta acctttgaaa ttcgatctcc agcacatcag
     4501 caaaacgcta ttcacgcagt acagcaaatc cttccagacc caaccaaacc aatcgtagta
     4561 accattcagg aacgcaaccg cagcttagac caaaacagga agctatgggc ctgcttaggt
     4621 gacgtctctc gtcaggttga atggcatggt cgctggctgg atgcagaaag ctggaagtgt
     4681 gtgtttaccg cagcattaaa gcagcaggat gttgttccta accttgccgg gaatggcttt
     4741 gtggtaatag gccagtcaac cagcaggatg cgtgtaggcg aatttgcgga gctattagag
     4801 cttatacagg cattcggtac agagcgtggc gttaagtggt cagacgaagc gagactggct
     4861 ctggagtgga aagcgagatg gggagacagg gctgcatgat aaatgtcgtt agtttctccg
     4921 gtggcaggac gtcagcatat ttgctctggc taatggagca aaagcgacgg gcaggtaaag
     4981 acgtgcatta cgttttcatg gatacaggtt gtgaacatcc aatgacatat cggtttgtca
     5041 gggaagttgt gaagttctgg gatataccgc tcaccgtatt gcaggttgat atcaacccgg
     5101 agcttggaca gccaaatggt tatacggtat gggaaccaaa ggatattcag acgcgaatgc
     5161 ctgttctgaa gccatttatc gatatggtaa agaaatatgg cactccatac gtcggcggcg
     5221 cgttctgcac tgacagatta aaactcgttc ccttcaccaa atactgtgat gaccatttcg
     5281 ggcgagggaa ttacaccacg tggattggca tcagagctga tgaaccgaag cggctaaagc
     5341 caaagcctgg aatcagatat cttgctgaac tgtcagactt tgagaaggaa gatatcctcg
     5401 catggtggaa gcaacaacca ttcgatttgc aaataccgga acatctcggt aactgcatat
     5461 tctgcattaa aaaatcaacg caaaaaatcg gacttgcctg caaagatgag gagggattgc
     5521 agcgtgtttt taatgaggtc atcacgggat ctaaacgccc accattccca cccttctggg
     5581 gccggacggc atgacatcgc tgcgcgaata tgccggttat cacggcggtg gcagcggatt
     5641 tggagggcag ttgcggtcgt ggaacccacc gagtgaaagt gtggatgcag ccctgttgcc
     5701 caactttacc cgtggcaatg cccgcgcaga cgatctggta cgcaataacg gctatgccgc
     5761 caacgccatc cagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg
     5821 cccaagctgg cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt
     5881 tgaagcggca tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa
     5941 acgcacgttt accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact
     6001 gttcgttcag gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat
     6061 ggtcagcccg aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc
     6121 cggtgtgcag attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta
     6181 tcctggctgg atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc
     6241 ctcgttcatt cacgtttttg aacccgtgga ggacgggcag actcgcggtg caaatgtgtt
     6301 ttacagcgtg atggagcaga tgaagatgct cgacacgctg cagaacacgc agctgcagag
     6361 cgccattgtg aaggcgatgt atgccgccac cattgagagt gagctggata cgcagtcagc
     6421 gatggatttt attctgggcg cgaacagtca ggagcagcgg gaaaggctga ccggctggat
     6481 tggtgaaatt gccgcgtatt acgccgcagc gccggtccgg ctgggaggcg caaaagtacc
     6541 gcacctgatg ccgggtgact cactgaacct gcagacggct caggatacgg ataacggcta
     6601 ctccgtgttt gagcagtcac tgctgcggta tatcgctgcc gggctgggtg tctcgtatga
     6661 gcagctttcc cggaattacg cccagatgag ctactccacg gcacgggcca gtgcgaacga
     6721 gtcgtgggcg tactttatgg ggcggcgaaa attcgtcgca tcccgtcagg cgagccagat
     6781 gtttctgtgc tggctggaag aggccatcgt tcgccgcgtg gtgacgttac cttcaaaagc
     6841 gcgcttcagt tttcaggaag cccgcagtgc ctgggggaac tgcgactgga taggctccgg
     6901 tcgtatggcc atcgatggtc tgaaagaagt tcaggaagcg gtgatgctga tagaagccgg
     6961 actgagtacc tacgagaaag agtgcgcaaa acgcggtgac gactatcagg aaatttttgc
     7021 ccagcaggtc cgtgaaacga tggagcgccg tgcagccggt cttaaaccgc ccgcctgggc
     7081 ggctgcagca tttgaatccg ggctgcgaca atcaacagag gaggagaaga gtgacagcag
     7141 agctgcgtaa tctcccgcat attgccagca tggcctttaa tgagccgctg atgcttgaac
     7201 ccgcctatgc gcgggttttc ttttgtgcgc ttgcaggcca gcttgggatc agcagcctga
     7261 cggatgcggt gtccggcgac agcctgactg cccaggaggc actcgcgacg ctggcattat
     7321 ccggtgatga tgacggacca cgacaggccc gcagttatca ggtcatgaac ggcatcgccg
     7381 tgctgccggt gtccggcacg ctggtcagcc ggacgcgggc gctgcagccg tactcgggga
     7441 tgaccggtta caacggcatt atcgcccgtc tgcaacaggc tgccagcgat ccgatggtgg
     7501 acggcattct gctcgatatg gacacgcccg gcgggatggt ggcgggggca tttgactgcg
     7561 ctgacatcat cgcccgtgtg cgtgacataa aaccggtatg ggcgcttgcc aacgacatga
     7621 actgcagtgc aggtcagttg cttgccagtg ccgcctcccg gcgtctggtc acgcagaccg
     7681 cccggacagg ctccatcggc gtcatgatgg ctcacagtaa ttacggtgct gcgctggaga
     7741 aacagggtgt ggaaatcacg ctgatttaca gcggcagcca taaggtggat ggcaacccct
     7801 acagccatct tccggatgac gtccgggaga cactgcagtc ccggatggac gcaacccgcc
     7861 agatgtttgc gcagaaggtg tcggcatata ccggcctgtc cgtgcaggtt gtgctggata
     7921 ccgaggctgc agtgtacagc ggtcaggagg ccattgatgc cggactggct gatgaacttg
     7981 ttaacagcac cgatgcgatc accgtcatgc gtgatgcact ggatgcacgt aaatcccgtc
     8041 tctcaggagg gcgaatgacc aaagagactc aatcaacaac tgtttcagcc actgcttcgc
     8101 aggatccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8161 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8221 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8281 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     8341 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8401 aatagtggac tcttgttcca aactggttta acactcagaa ttcgtaatca tggtcatagc
     8461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8641 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8701 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8761 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9481 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9601 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9661 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9721 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9781 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9841 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9901 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9961 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10021 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10081 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10141 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10201 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10261 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10321 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10381 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10441 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10501 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10561 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10621 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10681 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10741 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10801 ggtgtcgggg ctggcttaa
//
