LOCUS       sequence-209815-       12660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon 
            optimised for C. elegans) and mCherry under the promoter of choice 
            in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Alberti C, Manzenreither RA, Sowemimo I, Burkard TR, Wang J, 
            Mahofsky K, Ameres SL, Cochella L
  TITLE     Cell-type specific sequencing of microRNAs from complex animal 
            tissues.
  JOURNAL   Nat Methods. 2018 Feb 26. pii: nmeth.4610. doi: 10.1038/nmeth.4610.
  PUBMED    29481550
REFERENCE   2  (bases 1 to 12660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Feb 26. pii: nmeth.4610. doi: 10.1038/nmeth.4610."
COMMENT     Sequence Label: Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605
FEATURES             Location/Qualifiers
     source          1..12660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1559..1579
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3007..3031
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3038..3054
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3038..3054
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      3259..3268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3268..3315
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     regulatory      3331..3340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3340..3369
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6349..6402
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6403..7110
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6548..6566)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6816..6835
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8395..8412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8395..8411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10352..10363
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(10509..10533)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11869..11889)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     12212..12234
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12272..12290)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12358..12462
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12463..12531,12532..12660,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggtcag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca
     1561 actatgtata ataaagttgt gtttttgaac acggcgatat gtatctgtag atcatcccca
     1621 attttcaaca atgaatctaa atactgaact ttttgtgtaa tctttagtgc agaaaaatga
     1681 ccaaaatcag cgattttccg attataccca aaatatctca gctcaatgag cacctataaa
     1741 aatgtaaaaa taaagaaagt agtcgaacca tattttgtaa aggctaaaaa actgaataaa
     1801 tttttatcca tccagcgcgg cgttacaatc cgtggcccat gtcacgcacg gctttcaaag
     1861 aaaaaaaaac agtctgtttg cgcgacatcg atctggcaaa aatcctaaaa acgactatgc
     1921 gtggctcact ttatattaat atgaaaaata caaacttggt ggtgcaggtg ctgcagaggc
     1981 catcgatgaa gaagtggagg ccttgttgtc gatctacaaa attgtatggt tgtttgagat
     2041 tttccttgaa acgctctcaa tcttggatat gatataaatt ctggataaga tatgatggat
     2101 atgatctatg tttgatagag tgcatttgaa aggataacgg ccctgcagac atttttgaaa
     2161 aaataacaaa catttaattt tctcactcgt ttaggctatt cccctatttt tgatattcct
     2221 tcgcacatat gaaaactact ttttttcgaa actgttaact ccagaattta taaatctata
     2281 gcccttactt gattatttat tatcatggtt actcacctca tgtctctttc tcttttcaag
     2341 aagtcttcgt tcatatctgt tgtctctttc ctccttttga atctctgctt tgctcttcgc
     2401 cattgtttcc tgaaaataat gtaacttgaa ttgtgtaata tactttttta atttgaattt
     2461 ggcttgtaac gcggaatcac tacgtgcggg atcatttctt actagaaaac ccagaaaatg
     2521 ccatatttca cttatctcgg ggtcatttct aattagaaaa gctacaaaaa ccatttctaa
     2581 ttacggtgac tttagaactg gtcgaaatag ctaattagaa atgggttttt gctaattaga
     2641 ggtgactcta tgttatctga gtaagcctcg tttgtatgta aactcacagt actaataaat
     2701 gcaagacacc cgggtttgtc tagatatgaa ataattgaaa tatcaattct gacagacaat
     2761 aatggtaatc ttgataagga gttccacgcc caggagaaca cgttagtttt cttgtttttg
     2821 attgcgtgcg ttattttgga gaaaaactcg attttttaca aaataatttt ttgaaaggaa
     2881 cactgttcaa taagttttgt cttttttctc agttgtgata cggtttttta ttcttttttg
     2941 tagttataca gaagaccgtt acgaaacaga ctgatatcga aacaaacact gatcttactt
     3001 gcacttacca ctttgtacaa gaaagctggg tcacgggcag gaaacagcta tgaccatgat
     3061 tacgccaagc tacgtaatac gactcactag tgggcagatc ttcgaatgca tcgcgcgcac
     3121 cgtacgtctc gaggaattcc tgcaggatat ctggatccac gaagcttccc atggtgacgt
     3181 caccggttct agatacctag ggattacgcc aagcttgcat gcgcggccgc actgactggg
     3241 ccggccggcg cgccgctagc caccatggat tacaaggatg acgatgacaa ggactataag
     3301 gatgacgatg acaagcaccg tctctcctcc gccaccatgg aacagaaact catctctgaa
     3361 gaggatctga tggctggagg aggaaagcac accccaaccc caaaggctat catccaccaa
     3421 aagttcggag ccaaggcttc ctacaccgtc gaggaggtcc acgattcctc ccaatccgga
     3481 tgccttggac ttgctatccc acagaaggga ccatgccttt accgctgcca ccttcaactt
     3541 ccagagttct ccgtcgtttc taacgttttc aagaagaaga aggactccga gcaatccgcc
     3601 gccgagcttg cccttgacaa gcttggaatc cgtccacaaa acgacgacct caccgtcgac
     3661 gaggcccgtg acgagatcgt cggacgtatc aagtacatct tctccgacga gttcctctcc
     3721 gccgagcacc cacttggagc ccacctccgt gctgctcttc gccgcgacgg agagcgttgc
     3781 ggatctgtcc cagtctccgt catcgccacc gtcgacgcta agatcaactc tcgctgcaag
     3841 atcatcaacc catccgttga gtccgaccca ttccttgcta tctcctacgt catgaaggcc
     3901 gccgctaagc ttgctgatta catcgtcgct tccccacacg gacttcgccg taagaacgct
     3961 tacccatctg agatcgttga ggctcttgcc acccacgtct ccgactccct tcactcccgt
     4021 gaggttgctg ccgtttacat cccatgcatc gacgaagagg tcgtcgagct tgacaccctc
     4081 tacatctctt ctaaccgtca ctaccttgac tccatcgccg agcgtcttgg acttaaggta
     4141 agtttaaaca tatatatact aactaaccct gattatttaa attttcagga cggaaaccaa
     4201 gttatgatct ctcgcatgtt cggaaaggcc tcttgcggat ccgagtgccg tctttactcc
     4261 gaaatcccaa agaagtacct tgacaactcc tccgacgctt ccggaacctc taacgaggat
     4321 tcttcccaca tcgttaagtc ccgtaacgct cgtgcttctt acatctgcgg acaggacatc
     4381 cacggagacg ctatccttgc ttccgtcgga taccgttgga agtctgacga cctcgattac
     4441 gacgacgtta ccgttaactc tttctaccgc atttgctgtg gaatgtctcc aaacggaatt
     4501 tacaagatct cccgtcaagc cgtcatcgcc gctcaacttc cattcgcttt caccaccaag
     4561 tccaactggc gcggaccact tccacgcgag atccttggac ttttctgcca tcaacaccgt
     4621 cttgctgagc caatcctctc ctcctccacc gctccagtta agtccctttc tgatatcttc
     4681 cgctcccaca agaagcttaa ggtttccgga gtcgacgacg ctaacgagaa cctttctcgc
     4741 caaaaggagg acactccagg acttggacac ggattccgct gcgaggttaa gatcttcact
     4801 aagtcccaag accttgtcct tgagtgctct ccacgcaagt tctacgaaaa ggtaagttta
     4861 aacagttcgg tactaactaa ccatacatat ttaaattttc aggagaacga cgctatccaa
     4921 aacgcctccc ttaaggccct tctttggttc tctaagttct tcgccgatct tgacgttgac
     4981 ggagagcaat cttgcgacac cgacgatgat caagacacca agtcctcttc cccaaacgtc
     5041 ttcgctgctc caccaatcct tcaaaaggag cactcttccg agtctaagaa caccaacgtt
     5101 ctttccgctg agaagcgtgt ccaatccatc accaacggat ctgttgtctc tatctgctac
     5161 tccctttccc ttgctgttga cccagagtac tcttctgacg gagagtcccc acgtgaggat
     5221 aacgagtcta acgaggagat ggagtccgag tactctgcca actgcgagtc ttccgttgag
     5281 cttatcgagt ctaacgagga aatcgagttc gaggttggaa ccggatctat gaacccacac
     5341 atcgagtctg aggtcaccca aatgaccgtt ggagagtacg cctctttccg catgacccca
     5401 ccagacgccg ccgaggccct tatccttgcc gtcggatccg acaccgttcg tatccgttcc
     5461 cttctttctg agcgcccatg ccttaactac aacattctcc ttcttggagt taaggtaagt
     5521 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagggacc atctgaggag
     5581 cgcatggagg ctgccttctt caagccacca ctctctaagc aacgtgtcga gtacgctctt
     5641 aagcacatcc gtgagtcttc tgcctccacc cttgtcgatt tcggatgcgg atccggatct
     5701 cttcttgact cccttctcga ttacccaacc tctctccaaa ccatcatcgg agtcgatatc
     5761 tccccaaagg gactcgctcg tgccgccaag atgcttcacg ttaagcttaa caaggaggcc
     5821 tgcaacgtta agtccgccac cctctacgac ggatccatcc tcgagttcga ttcccgtctt
     5881 cacgatgtcg atatcggaac ctgccttgaa gtcatcgagc acatggagga ggaccaagct
     5941 tgcgagttcg gagagaaggt cctttccctt ttccacccaa agcttcttat cgtttctact
     6001 ccaaactacg aattcaacac tatccttcaa cgctccactc cagagactca agaggagaac
     6061 aactctgaac cacaactccc aaagttccgc aaccacgatc acaagttcga atggactcgc
     6121 gagcaattca accaatgggc ttctaagctt ggaaagcgtc acaactactc tgttgagttc
     6181 tctggagtcg gaggatccgg agaggttgag ccaggattcg cctcccaaat cgccatcttc
     6241 cgccgtgagg cctcctccgt cgagaacgtc gctgagtctt ctatgcaacc atacaaggtt
     6301 atctgggaat ggaagaagga ggacgttgag aagaagaaga ccgacctcga gggcagagga
     6361 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatggtgag caagggcgag
     6421 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     6481 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     6541 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     6601 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     6661 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     6721 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     6781 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     6841 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     6901 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     6961 aaggccaaga agcccgtgca gctacccggc gcctacaacg tcaacatcaa gttggacatc
     7021 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     7081 tccaccggcg gcatggacga gctgtacaag gaagtgcata ccaatcagga cccgctggat
     7141 ccaccaaagc catgtgccaa gggagccaag aaggccgcca agaccgttac gaagccaaag
     7201 gacggaaaga agagacgtca tgcccgtaag gaatcatact ccgtctacat ctaccgtgtc
     7261 ctcaagcaag ttcatccaga cactggagtt tcctccaaag ccatgtctat catgaactct
     7321 tttgtcaacg atgtcttcga gcgtattgct gctgaagcat cccgtcttgc tcactacaac
     7381 aagcgttcta ccatctcttc tcgcgaaatt cagaccgctg tccgtttgat tctcccagga
     7441 gaacttgcca agcacgccgt gtctgaggga accaaggccg tcaccaagta cacttccagc
     7501 aagtaatagg catggtattg atatctgagc tccgcatcgg ccgctgtcat cagatcgcca
     7561 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     7621 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     7681 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     7741 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     7801 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     7861 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     7921 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     7981 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     8041 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
     8101 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     8161 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     8221 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     8281 ctgtttctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg
     8341 cctaggtgag ctctggtacc ctctagtcaa ggccttaagt gagtcgtatt acggactggc
     8401 cgtcgtttta cacccgtgca gttgaaattg aaaatgagtt aaagttggag tttttattga
     8461 aaacagattt ccgtgtgatt agtgttttta gcgagtgtga caggacagcg aaaaaatata
     8521 gaaacaaggg gggaactgaa aagcttagga atgcattgaa catgagaagg ggaaggggaa
     8581 ggaacaaact agacaggaat tattggaatt taatcacatt tggagttttt tttctattcg
     8641 acagaataat tatccagaac atttttgtat taaatattta tgcatcatat gagtagtcgg
     8701 ctttgttgtg catgacgagt ttgttatcga cgaaatagaa gctgtcagaa cgagtctcgt
     8761 ttggattgtt gatcatgtcg tccactgaaa aagagattag tcttttgaat tgtacttttt
     8821 agaataatga ctcactgagt tgttgagaga gttgagggaa ctcatagata tgttcacagt
     8881 tgtttcgtga attcggaata cagaatccga attcaaagtc aaaacacttc agaaggcgat
     8941 ctttgaagaa gtgacgttcg atcattcgga aatgatggat tgggatgtct cctactttga
     9001 attccacagt tgctccgacc gttttgagtt tcaaaaagtt tggagcaaat ctataacgca
     9061 cgtatcttgc cgactcctgt ggcgactcat cattctcctg atcgttctcc ggtttggcga
     9121 tctcgaaaag cacttgctca gtgtccagat cacggatttg gaacttggtg aactcgatgt
     9181 tatagatgtt cgcagatggg gagcataaga atcctaaatt tatgttttaa actgaaatcc
     9241 aaagggagca agataccttg agtgattccc ggaagtgcta aaacgtcgtt cggagtgatt
     9301 tgagctttct tcgcaagctc cgattccgtt gtgattcctt gttcggtgct tggtggtggc
     9361 cgtggcatct ggaaatatgg aaaagttcaa caaaaagaaa agagaaaaga atgaaatcgg
     9421 atatcaagag ttagttgagc ggtttctcta gttttctgag tctcacctgc gacgggaagg
     9481 tcgccgagcc gggtggaatc gatcgttgtt gctcggcttt catatcggtt tggttggaag
     9541 cggctgaaaa cggaaagaag tggaagaagg aaaagagtgt ggtgtgacag gaaaatggta
     9601 attagagggt gccaaataac cagctatatt ttgttttttt ttgaaaacat ttttaaaaag
     9661 aaaaatacga taatgatatc agatggattt ccggaaaact ggtatgaaaa atttcaacct
     9721 ttttgagtac atgtaatcaa aatacacttt gtaaattatc atttttattg aaactccacc
     9781 atttttctat ttataacgct aataatttga aaaagaaacc tattgcgaac cgcggggtga
     9841 atcccaaaaa cgaatgcgtt ttggtggagt gattgattcg aatcgaagaa gaaaaagaag
     9901 aagacgtgga atagagagct cactcttaac cgagcagcac acaccgacag aaaaaaaaat
     9961 gaaatgaatg agggtcttct tcttcttctt cttcgaatga ttgacagaaa tgggaaaaag
    10021 aggaagattg agaagggaaa aaggaaggag aaaagaagca gaagaagacg tcagagagga
    10081 gaggaacgag cggaaaagca gcgggcgcaa gtcatagaag tagcagagct ggggagaaga
    10141 agacactatc caagaaagga atgacgagag agtatgcaaa ggggtatagg gtgcagacag
    10201 aataggaaca gaataacaga tgatgagcca agaagagttg aaaagggcga tgaatttgtc
    10261 atgtaactta atttgggtca atttgagcat gatgaattga aatcatccct tgttgggagt
    10321 taataaccgg tttgttatca gaaaccctgt aatagaaggg cgccctaact ttgagccaat
    10381 tcatcccggt ttctgtcaaa tatatcaaaa agtggtcaac tgacaaattg tttttgatat
    10441 tataataaac attttatccg ttaacaattt tcgaatactt tttacaagga cttggataaa
    10501 ttggctcaag cctgcttttt tgtacaaact tgacgaagtg agtttgctac catcatggaa
    10561 tcaggaggac atttcgacaa tgtcatataa tgtttgaaaa atgattttaa caagggtctc
    10621 attcgagata agcaatgctc tgagcaagtg gctcgtcgtc ttcaagaatc tcttgttgat
    10681 attcatttgg atcctgtttt gcacgaagca tgtgccatgg atattcagag tttttgcaaa
    10741 aaagttccgc caggtcaaac aatcttttta atttttatat gatacttcgg gtgtattttt
    10801 cttctttttc ctgttcattt tcgctttttt ctcacttttt tgtaacaaaa tctctcactt
    10861 ttctgtaagt cttaacaaaa gttcaattgg ctcgatcaag taaaatttcg gccgtatgaa
    10921 cgttttcaaa ggttttcatt tttttttaag ttcattcaca atatcatgaa tctctcgtcc
    10981 tttggtttcc ggtaagtagc gaaataatat aagcatactt ataaatgaag gaattgaata
    11041 tagaataagg aaagcataac taccaataac ggaatataga ggtaatactg taaatgtact
    11101 gataaccacc attacagtat tcattgaata tgccacagat tgagcaatac tacgatgttt
    11161 ttgaggtacc agttcagagg atataaacca cgaaattgga ccaactccag ttctgaaatt
    11221 tcattattcg attaaatcaa aatatatttg aaaaacctca cccataagta taaccgtaga
    11281 tgatcaatgc aactaggcat ccgtatttca tcgggtcaat cagaatacgt atttttgcga
    11341 agaatacgaa tgcaattaga gctaaattat tgagaaatgt gaagagaagg agaagcttgc
    11401 gtcgaccaac tctgtaaact tattcataca attttgtctt caaattttga acgtttaaaa
    11461 ctatcttatt caatgagtta gcgggttttt atagtgagtt agttcacgat tatctaataa
    11521 aactatgtcc aaacatccta actgcgaccg tattctcact aactcgtctt aaaaacgttg
    11581 agattatttt aaacccttct tcagtacaac tacagtactg atgcacaaat acaataataa
    11641 taatacattc agacatactc tgaacaatgt aacttgtttt tgttatactg tggtaacatt
    11701 tattgaaatg aaatataacc ttacttttca atcacaattc caccagtaat tgttccagaa
    11761 acataagcta aacccattgc agttgtactc cattcagcaa tttctgactc aacatttgca
    11821 ttttctagaa agaaagtaga ggataaaaga agagcccata atccgacaca acttttctat
    11881 acaaagttga tagcttggcg gatccctggc gttacccaac ttaatcgcct tgcagcacat
    11941 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    12001 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
    12061 ggtatttcac accgcatatg gtgcactctc agtgcaatct gctctgatgc cgcatagtta
    12121 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
    12181 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
    12241 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
    12301 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
    12361 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    12421 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    12481 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    12541 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    12601 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
//
