LOCUS       sequence-209816-        2838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry fused with silaffin-R5 silica affinity peptide 
            under the control of TetO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Olmez TT, Yuca E, Eyupoglu E, Catalak HB, Sahin O, Seker UOS
  TITLE     Autonomous Synthesis of Fluorescent Silica Biodots Using Engineered 
            Fusion Proteins.
  JOURNAL   ACS Omega. 2018 Jan 31;3(1):585-594. doi: 10.1021/acsomega.7b01769. 
            Epub 2018 Jan 18.
  PUBMED    30023783
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsomega.7b01769"; journalName: "ACS Omega"; date: 
            "2018-01-31- 31"; volume: "3"; issue: "1"; pages: "585-594"
COMMENT     Sequence Label: pZA-mCherry-R5
FEATURES             Location/Qualifiers
     source          1..2838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..54
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(75..93,94..99,100..116,117..122,123..148)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    75..93
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    100..118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             158..169
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1017..1103
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1138..1155)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1272..1817)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1352..1371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1931..2025)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2046..2705)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2620..2639
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2706..2808)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
       61 gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt gatagagata
      121 ctgagcacat cagcaggacg cactgaccga attcattaaa gaggagaaag gtacatggtt
      181 tccaagggcg aggaggataa catggctatc attaaagagt tcatgcgctt caaagttcac
      241 atggagggtt ctgttaacgg tcacgagttc gagatcgaag gcgaaggcga gggccgtccg
      301 tatgaaggca cccagaccgc caaactgaaa gtgactaaag gcggcccgct gccttttgcg
      361 tgggacatcc tgagcccgca atttatgtac ggttctaaag cgtatgttaa acacccagcg
      421 gatatcccgg actatctgaa gctgtctttt ccggaaggtt tcaagtggga acgcgtaatg
      481 aattttgaag atggtggtgt cgtgaccgtc actcaggact cctccctgca ggatggcgag
      541 ttcatctata aagttaaact gcgtggtact aattttccat ctgatggccc ggtgatgcag
      601 aaaaagacga tgggttggga ggcgtctagc gaacgcatgt atccggaaga tggtgcgctg
      661 aaaggcgaaa ttaaacagcg cctgaaactg aaagatggcg gccattatga cgctgaagtg
      721 aaaaccacgt acaaagccaa gaaacctgtg cagctgcctg gcgcgtacaa tgtgaatatt
      781 aaactggaca tcacctctca taatgaagat tatacgatcg tagagcaata tgagcgcgcg
      841 gagggtcgtc attctaccgg tggcatggat gagctgtaca aaggtggcgg tggcagtggc
      901 ggcggcggta gcggtggggg aggatctagc agcaaaaaaa gcggcagcta tagcggcagc
      961 aaaggcagca aacgccgcat tctgtaagga tcccatggta cgcgtgctag aggcatcaaa
     1021 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1081 acgctctcct gagtaggaca aatccgccgc cctagaccta gggatatatt ccgcttcctc
     1141 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     1201 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     1261 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     1321 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     1381 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     1441 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     1501 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     1561 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     1621 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     1681 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     1741 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     1801 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
     1861 tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgttac
     1921 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1981 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     2041 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     2101 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     2161 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     2221 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     2281 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     2341 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     2401 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     2461 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     2521 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     2581 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2641 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     2701 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     2761 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     2821 cgccagatat cgacgtct
//
