LOCUS       sequence-209817-        4587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Elongin B and Elongin C co-expression plasmid for structure 
            determination; Full length Elongin B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ELONGIN BC (XX01TCEB1A-c001)
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        502..520
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    521..545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             943..987
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(1025..1043)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1039..1086
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1307..1966)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1881..1900
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1967..2069)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2461..2478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2595..3140)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2675..2694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3365..4447)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4409..4428
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4448..4525)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(4571..4587,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggacgtgt ttctcatgat ccggcgccac aagaccacca tcttcacgga
      121 cgccaaggag tccagcacgg tgttcgaact gaagcgcatc gtcgagggca tcctcaagcg
      181 gcctcctgac gagcagcggc tgtacaagga tgaccaactc ttggatgatg gcaagacact
      241 gggcgagtgt ggcttcacca gtcaaacagc acggccacag gccccagcca cagtggggct
      301 ggccttccgg gcagatgaca ccttcgaggc cctgtgcatc gagccgtttt ccagcccgcc
      361 agagctgccc gatgtgatga agccccagga ctcgggaagc agtgccaatg aacaagctgt
      421 gcagtagggg aagcttgcgg ccgcataatg cttaagtcga acagaaagta atcgtattgt
      481 acacggccgc ataatcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
      541 attccccatc ttagtatatt agttaagtat aagaaggaga tatacatatg atgtatgtca
      601 aattgatatc atctgatggc catgaattta ttgtaaaaag agaacatgca ttaacatcag
      661 gcacgataaa agccatgttg agtggcccag gtcagtttgc tgagaacgaa accaatgagg
      721 tcaattttag agagatacct tcacatgtgc tatcgaaagt atgcatgtat tttacgtaca
      781 aggttcgcta cactaacagc tccaccgaga ttcctgaatt cccaattgca cctgaaattg
      841 cactggaact gctgatggct gcgaacttct tagattgtta gggatctcaa ttggatatcg
      901 gccggccacg cgatcgctga cgtcggtacc ctcgagtctg gtaaagaaac cgctgctgcg
      961 aaatttgaac gccagcacat ggactcgtct actagcgcag cttaattaac ctaggctgct
     1021 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     1081 tttttgctga aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat
     1141 aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg
     1201 accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg
     1261 cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     1321 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1381 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1441 atttgcccat agtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1501 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     1561 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1621 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1681 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     1741 ccatacggaa ctccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1801 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1861 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1921 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1981 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2041 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     2101 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     2161 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta
     2221 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
     2281 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt
     2341 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     2401 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     2461 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     2521 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     2581 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     2641 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     2701 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     2761 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     2821 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     2881 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     2941 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     3001 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     3061 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     3121 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt
     3181 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     3241 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     3301 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     3361 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     3481 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     3541 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     3601 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc
     3661 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     3721 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     3781 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     3841 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     3901 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     3961 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     4021 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     4081 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     4141 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     4201 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     4261 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     4321 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     4381 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     4441 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
     4501 aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc
     4561 attaggaaat taatacgact cactata
//
