LOCUS       sequence-209821-        6578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Ohman T, 
            Varjosalo M
  TITLE     An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping
            of protein interactions and subcellular localizations.
  JOURNAL   Nat Commun. 2018 Mar 22;9(1):1188. doi: 10.1038/s41467-018-03523-2.
  PUBMED    29568061
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-22"; volume: "9(1):1188. doi"; pages: " 
            10.1038/s41467-018-03523-2"
COMMENT     Sequence Label: MAC_N_MED13
FEATURES             Location/Qualifiers
     source          1..6578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1112..1121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6554..6578)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acaagtttgt acaaaaaagc aggcttcacc atgagtgcct ccttcgtgcc gaacggggcc
       61 agcctggaag attgtcactg taacctcttc tgcctggctg acttgacagg aattaagtgg
      121 aaaaaatatg tatggcaagg cccaacttct gcccctattc tgtttcctgt gacagaagaa
      181 gaccccattt tgagcagttt tagtcgctgc cttaaggcag atgtacttgg tgtttggcgg
      241 cgagatcaaa gacctggaag aagagaattg tggatatttt ggtggggtga agaccccagt
      301 tttgctgacc ttattcacca tgacttatca gaagaagaag atggagtgtg ggagaatgga
      361 ctttcctatg aatgccgtac tctgcttttc aaagcagttc acaatctatt ggaacggtgt
      421 ttaatgaaca ggaattttgt acgtattggc aagtggtttg taaagcctta tgaaaaagat
      481 gaaaaaccta taaataaaag tgaacacttg tcctgctcct tcaccttttt cttgcatgga
      541 gacagcaatg tttgtaccag tgtggaaatt aaccaacatc aacctgtata ccttctcagt
      601 gaagagcata tcacccttgc tcaacagtct aatagcccat ttcaagttat cttatgccca
      661 tttggactaa atggcactct cacaggacag gcattcaaga tgtctgattc agctacaaaa
      721 aaattaattg gtgaatggaa acagttctat cctatctcat gttgcttgaa ggagatgtct
      781 gaagaaaaac aggaagatat ggattgggaa gatgattctt tagctgcagt agaagttctt
      841 gttgctggtg tccgaatgat ctacccagca tgctttgttc tagtccctca gtcagacatt
      901 cctactccta gccctgtggg atccactcac tgttcatctt cttgcttggg tgtccaccaa
      961 gtgcctgctt ccacaagaga tcctgctatg tcttcggtta cgcttacacc acctacgtct
     1021 cctgaggaag tccaaacagt tgatcctcag tctgtccaga agtgggtcaa attttcttca
     1081 gtatctgatg gcttcaactc cgatagtact agccaccatg gtgggaaaat acccagaaaa
     1141 ttagcaaatc atgtggtgga tagagtttgg caagaatgca atatgaacag agcacagaac
     1201 aagaggaagt attctgcttc atcaggtggt ctatgcgaag aagcgacagc tgctaaagtg
     1261 gcatcctggg attttgttga agccacacaa agaacaaatt gcagttgttt gaggcacaaa
     1321 aatctcaagt caagaaatgc tggacaacaa ggacaggcac catctttagg tcagcaacaa
     1381 caaatacttc ctaagcacaa gaccaatgag aagcaagaaa agagtgaaaa gccacagaaa
     1441 cgccccttga ctccttttca ccatcgtgtg tctgttagtg atgatgttgg catggacgca
     1501 gattcagcca gccaaagact tgtgatctct gctccagaca gtcaagtgag attttcaaat
     1561 atccgaacta atgatgtagc aaagactcct cagatgcatg gcaccgaaat ggcaaattca
     1621 cctcaaccac ccccacttag tcctcaccct tgtgatgtgg ttgatgaagg agtgactaaa
     1681 acaccttcaa ctcctcagag tcaacatttt tatcaaatgc caacaccaga tcccttggtt
     1741 ccttctaaac caatggaaga taggatagac agtttgtccc agtctttccc acctcaatat
     1801 caggaagctg tagaacctac agtatatgtt ggtacagcag taaacttgga agaagatgaa
     1861 gccaatatag cctggaagta ttacaagttc ccaaagaaaa aagatgtaga gtttttacca
     1921 cctcaacttc caagtgataa attcaaggat gatccagttg gaccttttgg acaggaaagt
     1981 gtaacatcag ttacagagtt aatggtgcaa tgtaagaaac ctttaaaagt ttctgatgaa
     2041 ttagtgcagc aatatcaaat taaaaaccag tgtctttcag caatagcatc tgatgcagaa
     2101 caagaaccta aaattgatcc atatgcattt gttgaaggag atgaggaatt cctttttcct
     2161 gataaaaaag atagacaaaa tagtgagaga gaagctggaa aaaaacacaa ggtagaagat
     2221 gggacatcta gtgtaacagt gttatcacat gaagaagatg ctatgtcatt atttagtccc
     2281 tctatcaagc aagatgctcc acgccctact agtcatgccc gtcctccatc aacaagtttg
     2341 atttatgact cagacctggc tgtctcttat actgaccttg ataatctctt caattctgat
     2401 gaagatgaac taacacctgg atctaaaaaa tcagcaaatg gatcagatga taaagccagc
     2461 tgcaaggaat caaagacagg aaatctggac ccgttatctt gcataagcac tgcagatctt
     2521 cataaaatgt atcctacacc accatcattg gaacaacata ttatgggatt ttccccaatg
     2581 aatatgaata ataaagaata tggtagtatg gatacaacac ctggaggaac tgttctagaa
     2641 ggaaatagtt ctagtatagg agcgcagttc aaaattgagg ttgatgaggg attctgtagc
     2701 cccaaacctt ctgaaattaa agatttttct tatgtctata agcctgaaaa ttgtcaaatt
     2761 ctagtgggat gttccatgtt tgcacctcta aaaactctac caagccaata tctgccccct
     2821 atcaaattgc cagaagagtg tatttaccgt cagagttgga ctgttggaaa attggaattg
     2881 ctttcttcag ggccttcaat gccattcatc aaagagggtg atggaagtaa tatggatcaa
     2941 gaatatggca ctgcttatac acctcaaact catacttctt ttgggatgcc tcctagcagt
     3001 gcacctccta gtaacagcgg agcaggaatt cttccttctc catccacccc tcggtttcca
     3061 actccaagga ctccaaggac tcctcggact cctcgtggag ctggtggacc tgctagtgct
     3121 caaggttcag tcaaatatga aaattcagac ttgtattcac cagcttctac cccatctaca
     3181 tgcagacccc ttaattctgt tgaacctgca actgtccctt ccatccctga agcacacagt
     3241 ctttatgtaa acctcatcct ttcagaatca gttatgaatt tgtttaaaga ctgtaacttt
     3301 gatagttgtt gcatctgtgt ttgcaacatg aacatcaagg gtgccgatgt tggagtttac
     3361 attccagatc caacgcagga agcacaatat aggtgtacct gtggcttcag tgctgtcatg
     3421 aacagaaaat ttggaaacaa ttcaggatta tttcttgaag atgaactaga tatcatagga
     3481 cgcaatacag actgtggcaa agaagcagaa aaacgttttg aagctctcag ggctacctct
     3541 gctgaacatg ttaatggagg actaaaggaa tctgaaaaat tatctgatga tttgatatta
     3601 ttgctacaag atcagtgcac taatttattt tcaccctttg gagcagcaga ccaagatcct
     3661 tttcctaaaa gtggtgtaat tagcaattgg gtacgtgttg aagagcgtga ctgttgcaat
     3721 gactgctacc ttgcattaga acatgggcgt cagttcatgg ataacatgtc aggaggaaaa
     3781 gttgatgaag cacttgtgaa aagttcatgc ttacacccct ggtccaaaag aaacgatgtg
     3841 agtatgcagt gctcacagga tatacttcga atgctcctct ctcttcagcc agttcttcag
     3901 gatgccattc agaaaaaaag aacagtaaga ccttggggtg ttcagggtcc tctcacttgg
     3961 caacaatttc ataaaatggc tggccgaggc tcttatggaa ctgatgaatc cccagaacca
     4021 ctgccaatcc ccacattttt gttgggttat gattatgatt atctggtgct ttctccattt
     4081 gctcttcctt attgggagag acttatgctg gaaccctatg gatctcaaag agatatagcc
     4141 tatgttgtac tgtgtccaga aaatgaagcc ttgttaaatg gagcaaaaag cttttttaga
     4201 gatcttactg caatatatga gtcctgtcga ttaggtcaac atagacctgt ttctcgactg
     4261 ttaacagatg ggatcatgag agttggatct actgcatcaa agaaactatc agaaaagttg
     4321 gtagcagaat ggttttctca ggcagctgac ggtaacaatg aagcattttc taaactcaag
     4381 ctttatgcac aagtctgcag atatgaccta ggtccttatc ttgcttccct gccattggac
     4441 agctctctac tttcccagcc aaatttagtt gcccctacaa gtcagtcttt gattactcca
     4501 cctcagatga caaatactgg aaatgctaat actccatctg ccaccttagc atctgcagcg
     4561 agcagcacta tgacagtgac ttcaggtgtt gccatatcta cttcagttgc cacagctaat
     4621 tcaactttga ccacagcttc aacttcatct tcatcatcct ccaacttgaa tagtggagta
     4681 tcatcaaata aactaccttc gtttccaccc tttggcagta tgaacagtaa tgctgcagga
     4741 tccatgtcta cacaagcaaa tacagttcag agtggtcagc taggagggca acagacatca
     4801 gctctacaga cagctgggat ttctggagaa tcatcttcac ttcccactca gccgcatcct
     4861 gatgtgtctg aaagcacgat ggatcgggat aaagtgggaa tccccacaga tggtgattca
     4921 catgcagtca cgtatccacc tgcaattgtt gtttatataa ttgatccttt tacatacgaa
     4981 aatacagacg agagcactaa ctcttctagt gtgtggacat tggggctact tcgatgcttt
     5041 ctagaaatgg tccagactct tcctcctcat atcaagagta ctgtttctgt acagattatt
     5101 ccttgtcagt acctgttgca acctgtgaag catgaagata gagaaatcta tccccagcat
     5161 ttaaaatccc tggctttttc ggcctttacc cagtgtcgga ggccacttcc aacatcaacc
     5221 aatgtgaaaa cattgactgg ctttggtcca ggtttagcca tggaaactgc ccttagaagt
     5281 cctgatagac cagagtgtat tcgactttat gcacctcctt ttattctggc tccagtgaag
     5341 gacaaacaga cagagctagg agaaacattt ggagaagctg gacagaaata taatgttctt
     5401 tttgtgggat actgtttatc acatgatcaa aggtggattc ttgcatcttg cacagatcta
     5461 tatggagaac ttttagaaac ttgtatcatt aacatcgatg ttccaaatag ggctcgtcgg
     5521 aaaaaaagtt ctgctagaaa atttggtcta cagaaacttt gggagtggtg cttaggactt
     5581 gtacaaatga gttcattgcc atggagagtt gtaattggtc gtctaggaag gattggtcat
     5641 ggagaattga aagattggag ctgtttgctg agtcgtcgaa acttgcagtc tctaagtaaa
     5701 aggctcaaag acatgtgtag aatgtgtggt atatctgctg cagactcccc tagcattctc
     5761 agtgcttgct tggtggcaat ggagccgcaa ggctcttttg ttattatgcc agattctgtg
     5821 tcaactggtt ctgtatttgg aagaagcacg actctaaata tgcagacatc tcagctaaat
     5881 accccacagg atacatcatg tactcatata cttgtgtttc ctacttctgc ttctgtgcaa
     5941 gtagcttcag ctacttatac cactgaaaat ttggatttag ctttcaatcc caacaatgat
     6001 ggagcagatg gaatgggtat ctttgatttg ttagacacag gagatgatct tgaccctgat
     6061 atcattaata tccttcctgc ttctccaact ggttctcctg tacattctcc aggatctcat
     6121 tacccccatg gaggtgatgc gggcaagggt cagagtactg atcggctact atcaacagaa
     6181 cctcatgagg aagtacctaa tattcttcag caaccattgg cccttggtta ctttgtatca
     6241 actgccaaag caggtccatt acctgactgg ttctggtcag catgtcctca agcacaatat
     6301 cagtgtcccc tttttcttaa ggcctctttg cacctccacg tgccttcagt gcaatctgac
     6361 gagctgcttc acagtaaaca ctcccaccca cttgactcaa atcagacttc agatgtcctc
     6421 aggtttgttt tggaacagta caatgcactc tcctggctaa cctgtgaccc tgcaacccag
     6481 gacagacgct catgtctccc aattcatttt gtggtgctga atcagttata taactttatt
     6541 atgaatatgc tggacccagc tttcttgtac aaagtggt
//
