LOCUS       sequence-209825-       13599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13599)
  AUTHORS   Tuttle JR, Haigler CH, Robertson D
  TITLE     Method: low-cost delivery of the cotton leaf crumple virus-induced 
            gene silencing system.
  JOURNAL   Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.
  PUBMED    22853641
REFERENCE   2  (bases 1 to 13599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2012-08-1"; volume: "8(1):27. doi"; pages: " 
            10.1186/1746-4811-8-27"
COMMENT     Sequence Label: pJRT.Agro.CLCrVB1.3
FEATURES             Location/Qualifiers
     source          1..13599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(33..709)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(76..96)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    900..921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(936..941,942..959,960..966)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    974..990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     979..1001
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     998..1014
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     998..1014
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4525..4542)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4525..4541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4534..4556)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4744..4768
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6069..6698
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7127..8200
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8266..8460
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8696..8718)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8804..8944
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8859..8878
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8959..8976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9130..9718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9210..9229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11004..11798)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12223..12247
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12325..12499
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12540..13565)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
ORIGIN
        1 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
       61 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
      121 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
      181 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
      241 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
      301 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
      361 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
      421 ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga
      481 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
      541 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
      601 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
      661 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
      721 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
      781 gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      841 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      901 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      961 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
     1021 acgaattcga gctccagaac gatctcagtt aggtcatggg aaagctgata ttcatccctc
     1081 tgagactcta tataattaaa tgcgtttggg ggattagcta attgagaatc catatagaga
     1141 aatatggccg cgcagcgacg ccggttgaag aatatgaaca aggaataaga tgaacaagga
     1201 agaagatgaa caagcaataa tttaattaat tattgttaat aagcataaat tatgaaaata
     1261 attttgatgg taatgaaagt attataaata atatttagga gaaaatattg ttaaggaaat
     1321 atctggttat gtttatttgt taaggtatat gggtttttat agaaacccag gagcagctat
     1381 ggtaagaaac ccaggactca aaaaatgcct ctcaacttct ctcatatgtt gcggagtctg
     1441 gagtcccatt tataccatag aaagcattgg gggtatagca tggtaatttc acatcggact
     1501 ccataccgcg gccatctata taatattact gatggccgcg cgaagtcccg gtatgccttt
     1561 tcagccgttg gatctggagc cgtacctttt tcgcgttccc attttgcccc tggagtcccg
     1621 agctatttac gaccccctgc caccgagtcc ccttcgtgga gcgctcttcg tccaacacgt
     1681 gtcccaactt ttgcgcattt ttgactacct gtcgcgcact tgaattaaag ttgccctttt
     1741 attttcttcc aattattttg cggctgagta gtctagataa ttgtgacaac ttagtgagca
     1801 agttgtagga ggaccctatc cgtctgggaa aataattggg ttttcacgtg taccaatggg
     1861 aaataatatg tatagcctat ttaatttaga aaattaaata gttttagtaa ttcctatata
     1921 atagcgttta ctagtctggg taaaattcgt ataggaaaaa atatttatta ttttttaaat
     1981 ttaacctatt gaaaatgtac agtttaaaga gtagacgtgg atcgaattac attcaagcac
     2041 gtggaaactc acggacccca ttattcaaac gacattccac tggtaagcgt tatggaggaa
     2101 gacgactaca gccactgaat gtgaaaagag aagatgaacc cagaatgtcg acccaacgca
     2161 tccacgagaa tcaatatggg tcggaattta caatggccca caacacagcc atttcgacct
     2221 atatctccta tcctaatctg ggcaagactg aacccaatcg aaccaggtcg tacatcaagc
     2281 tgaaacgatt gcgtttcaag ggcacggtga agatcgaacg agttcctact gacgtggcta
     2341 tggatggttc gattcccaac acggaagggg tgttctcatt ggtcatagtc gttgaccgaa
     2401 aacctcattt gactgcatct ggatgcctac atacgtttga cgaactattt ggtgcgataa
     2461 tccacagcca tggaaccctg tctatagttc ccgcgttgaa ggatcgttac tacatacggc
     2521 atgtaattaa acgggtactg tccgtcgaga aagataacgt catggtggat attgaaggat
     2581 ccactgctct gtctaatagg cgttttaatt gttgggccag ttttaaagat cttgatttgg
     2641 attcatgtaa gggtgtttat gacaacataa acaagaatgc cctgttagtt tattactcat
     2701 gggtatcgga tactgtgtct aaagcatcca cttatgtatc gtatgacctt gattatattg
     2761 gataatcaat aataacagga aaatttgaat aagaaaaaat attatttaaa tatttttggt
     2821 tgaggaccat tacaactact cttgatacat tcttggaccg ttgtcctaac cagttcgttt
     2881 aattgggcca tagacatggt aatgttggac tcagccctat gggcccctac tattgatgcc
     2941 gagtcacctg gatccagtac agtggaccct aacctgtgca gctctctgta aggtgtctgt
     3001 tcgttattgg actgtgagtc cgcatcttcg ttaacgggcc ctatggtact cctggtcgcc
     3061 cacgactccc ctggccttat ttctattggg cctggaagcc caaatctgtt catggatgcg
     3121 gacctaatta gtttcctttc ccatttacca tagtctacat gtgagaaatc aacgtcttta
     3181 gcggagaact gtttggaaag gatctttacc gttggcgcct tgaacggaat atcgacggaa
     3241 tgtttggcgg tcgataattt cagcttccct ttgaattttg cgaagtgggt acgctgttga
     3301 acattcgtat cagaaactct gtaatagagt ttccaaggga ttgggtcttt gagagagaag
     3361 aaggaagctg aaaaatagtg gagatctatg ttacacctga tcggaaaagt ccacgacgat
     3421 tgtaatgatt cattatcagt catcctcttg tcgtgaatct ccactatcac tgtaccagtc
     3481 gcgttaattg gcacctgctg cctgtactcg ataacgcaat gatcgatctt catgcaactg
     3541 cgactgagtc tcgccgttaa ttgtgccgcc gttgacggaa actgcagaac gatctcagtt
     3601 aggtcatggg aaagctgata ttcatccctc tgagactcta tataattaaa tgcgtttggg
     3661 ggattagcta attgagaatc catatagaga aatatggccg cgcagcgacg ccggttgaag
     3721 aatatgaaca aggaataaga tgaacaagga agaagatgaa caagcaataa tttaattaat
     3781 tattgttaat aagcataaat tatgaaaata attttgatgg taatgaaagt attataaata
     3841 atatttagga gaaaatattg ttaaggaaat atctggttat gtttatttgt taaggtatat
     3901 gggtttttat agaaacccag gagcagctat ggtaagaaac ccaggactca aaaaatgcct
     3961 ctcaacttct ctcatatgtt gcggagtctg gagtcccatt tataccatag aaagcattgg
     4021 gggtatagca tggtaatttc acatcggact ccataccgcg gccatctata taatattact
     4081 gatggccgcg cgaagtcccg gtatgccttt tcagccgttg gatctggagc cgtacctttt
     4141 tcgcgttccc attttgcccc tggagtcccg agctatttac gaccccctgc caccgagtcc
     4201 ccttcgtgga gcgctcttcg tccaacacgt gtcccaactt ttgcgcattt ttgactacct
     4261 gtcgcgcact tgaattaaag ttgccctttt attttcttcc aattattttg cggctgagta
     4321 gtctagataa ttgtgacaac ttagtgagca agttgtagga ggaccctatc cgtctgggaa
     4381 aataattggg ttttcacgtg taccaatggg aaataatatg tatagcctat ttaatttaga
     4441 aaattaaata gttttagtaa ttcctatata atagcgttta ctagtggatc ccccgggctg
     4501 caggaattcg atatcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     4561 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     4621 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4681 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
     4741 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     4801 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     4861 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     4921 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     4981 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     5041 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     5101 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     5161 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     5221 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     5281 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     5341 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     5401 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     5461 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     5521 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     5581 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     5641 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     5701 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     5761 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     5821 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     5881 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     5941 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     6001 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     6061 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     6121 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     6181 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     6241 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     6301 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     6361 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     6421 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     6481 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     6541 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     6601 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     6661 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     6721 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     6781 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     6841 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     6901 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     6961 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     7021 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     7081 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     7141 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     7201 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     7261 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     7321 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     7381 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     7441 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     7501 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     7561 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     7621 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     7681 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     7741 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     7801 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     7861 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     7921 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     7981 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     8041 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     8101 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     8161 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     8221 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
     8281 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     8341 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     8401 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     8461 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     8521 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     8581 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     8641 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     8701 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     8761 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     8821 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     8881 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     8941 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9541 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9781 gattttggtc atgcattcta gggaaggtgc gaacaaatcc ctgatatgag atcatgtttg
     9841 tcatctggag ccatagaaca gggttcatca tgagtcatca acttaccttc gccgacagtg
     9901 aattcagcag taagcgccgt cagaccagaa aagagatttt cttgtcccgc atggagcaga
     9961 ttctgccatg gcaaaacatg gtggaagtca tcgagccgtt ttaccccaag gctggtaatg
    10021 gccggcgacc ttatccgctg gaaaccatgc tacgcattca ctgcatgcag cattggtaca
    10081 acctgagcga tggcgcgatg gaagatgctc tgtacgaaat cgcctccatg cgtctgtttg
    10141 cccggttatc cctggatagc gccttgccgg accgcaccac catcatgaat ttccgccacc
    10201 tgctggagca gcatcaactg gcccgccaat tgttcaagac catcaatcgc tggctggccg
    10261 aagcaggcgt catgatgact caaggcacct tggtcgatgc caccatcatt gaggcaccca
    10321 gctcgaccaa gaacaaagag cagcaacgcg atccggagat gcatcagacc aagaaaggca
    10381 atcagtggca ctttggcatg aaggcccaca ttggtgtcga tgccaagagt ggcctgaccc
    10441 acagcctggt caccaccgcg gccaacgagc atgacctcaa tcagctgggt aatctgctgc
    10501 atggagagga gcaatttgtc tcagccgatg ccggctacca aggggcgcca cagcgcgagg
    10561 agctggccga ggtggatgtg gactggctga tcgccgagcg ccccggcaag gtaagaacct
    10621 tgaaacagca tccacgcaag aacaaaacgg ccatcaacat cgaatacatg aaagccagca
    10681 tccgggccag ggtggagcac ccatttcgca tcatcaagcg acagttcggc ttcgtgaaag
    10741 ccagatacaa ggggttgctg aaaaacgata accaactggc gatgttattc acgctggcca
    10801 acctgtttcg ggcggaccaa atgatacgtc agtgggagag atctcactaa aaactgggga
    10861 taacgcctta aatggcgaag aaacggtcta aataggctga ttcaaggcat ttacgggaga
    10921 aaaaatcggc tcaaacatga agaaatgaaa tgactgagtc agccgagaag aatttccccg
    10981 cttattcgca ccttccctag gtactaaaac aattcatcca gtaaaatata atattttatt
    11041 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
    11101 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
    11161 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
    11221 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
    11281 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
    11341 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
    11401 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
    11461 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
    11521 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
    11581 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
    11641 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
    11701 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
    11761 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
    11821 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
    11881 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
    11941 tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa
    12001 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
    12061 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
    12121 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
    12181 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
    12241 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
    12301 aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa
    12361 ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat
    12421 atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc
    12481 acacattatt atggagaaac tcgagcttgt cgatcgacag atcccggtcg gcatctactc
    12541 tatttctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct
    12601 acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc
    12661 ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa
    12721 ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg
    12781 agtcgtggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc
    12841 atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg
    12901 aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg
    12961 ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc
    13021 atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc
    13081 cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga
    13141 ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg
    13201 atcgcatcca tagcctccgc gaccggttgt agaacagcgg gcagttcggt ttcaggcagg
    13261 tcttgcaacg tgacaccctg tgaacggcgg gagatgcaat aggtcaggct ctcgctaaac
    13321 tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca
    13381 taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct
    13441 cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag
    13501 acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt
    13561 ttcatatctc attgcccccc ggatctgcga aagctcgag
//
