LOCUS       sequence-209874-        9432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8750..8759
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 ccgccaccgc cgccgccgcc gccgccgcct cctcagcttc ctcagccgcc gccgcaggca
      181 cagccgctgc tgcctcagcc gcagccgccc ccgccgccgc ccccgccgcc acccggcccg
      241 gctgtggctg aggagccgct gcaccgacca aagaaagaac tttcagctac caagaaagac
      301 cgtgtgaatc attgtctgac aatatgtgaa aacatagtgg cacagtctgt cagaaattct
      361 ccagaatttc agaaacttct gggcatcgct atggaacttt ttctgctgtg cagtgatgac
      421 gcagagtcag atgtcaggat ggtggctgac gaatgcctca acaaagttat caaagctttg
      481 atggattcta atcttccaag gttacagctc gagctctata aggaaattaa aaagaatggt
      541 gcccctcgga gtttgcgtgc tgccctgtgg aggtttgctg agctggctca cctggttcgg
      601 cctcagaaat gcaggcctta cctggtgaac cttctgccgt gcctgactcg aacaagcaag
      661 agacccgaag aatcagtcca ggagaccttg gctgcagctg ttcccaaaat tatggcttct
      721 tttggcaatt ttgcaaatga caatgaaatt aaggttttgt taaaggcctt catagcgaac
      781 ctgaagtcaa gctcccccac cattcggcgg acagcggctg gatcagcagt gagcatctgc
      841 cagcactcaa gaaggacaca atatttctat agttggctac taaatgtgct cttaggctta
      901 ctcgttcctg tcgaggatga acactccact ctgctgattc ttggcgtgct gctcaccctg
      961 aggtatttgg tgcccttgct gcagcagcag gtcaaggaca caagcctgaa aggcagcttc
     1021 ggagtgacaa ggaaagaaat ggaagtctct ccttctgcag agcagcttgt ccaggtttat
     1081 gaactgacgt tacatcatac acagcaccaa gaccacaatg ttgtgaccgg agccctggag
     1141 ctgttgcagc agctcttcag aacgcctcca cccgagcttc tgcaaaccct gaccgcagtc
     1201 gggggcattg ggcagctcac cgctgctaag gaggagtctg gtggccgaag ccgtagtggg
     1261 agtattgtgg aacttatagc tggagggggt tcctcatgca gccctgtcct ttcaagaaaa
     1321 caaaaaggca aagtgctctt aggagaagaa gaagccttgg aggatgactc tgaatcgaga
     1381 tcggatgtca gcagctctgc cttaacagcc tcagtgaagg atgagatcag tggagagctg
     1441 gctgcttctt caggggtttc cactccaggg tcagcaggtc atgacatcat cacagaacag
     1501 ccacggtcac agcacacact gcaggcggac tcagtggatc tggccagctg tgacttgaca
     1561 agctctgcca ctgatgggga tgaggaggat atcttgagcc acagctccag ccaggtcagc
     1621 gccgtcccat ctgaccctgc catggacctg aatgatggga cccaggcctc gtcgcccatc
     1681 agcgacagct cccagaccac caccgaaggg cctgattcag ctgttacccc ttcagacagt
     1741 tctgaaattg tgttagacgg taccgacaac cagtatttgg gcctgcagat tggacagccc
     1801 caggatgaag atgaggaagc cacaggtatt cttcctgatg aagcctcgga ggccttcagg
     1861 aactcttcca tggcccttca acaggcacat ttattgaaaa acatgagtca ctgcaggcag
     1921 ccttctgaca gcagtgttga taaatttgtg ttgagagatg aagctactga accgggtgat
     1981 caagaaaaca agccttgccg catcaaaggt gacattggac agtccactga tgatgactct
     2041 gcacctcttg tccattgtgt ccgcctttta tctgcttcgt ttttgctaac agggggaaaa
     2101 aatgtgctgg ttccggacag ggatgtgagg gtcagcgtga aggccctggc cctcagctgt
     2161 gtgggagcag ctgtggccct ccacccggaa tctttcttca gcaaactcta taaagttcct
     2221 cttgacacca cggaataccc tgaggaacag tatgtctcag acatcttgaa ctacatcgat
     2281 catggagacc cacaggttcg aggagccact gccattctct gtgggaccct catctgctcc
     2341 atcctcagca ggtcccgctt ccacgtggga gattggatgg gcaccattag aaccctcaca
     2401 ggaaatacat tttctttggc ggattgcatt cctttgctgc ggaaaacact gaaggatgag
     2461 tcttctgtta cttgcaagtt agcttgtaca gctgtgagga actgtgtcat gagtctctgc
     2521 agcagcagct acagtgagtt aggactgcag ctgatcatcg atgtgctgac tctgaggaac
     2581 agttcctatt ggctggtgag gacagagctt ctggaaaccc ttgcagagat tgacttcagg
     2641 ctggtgagct ttttggaggc aaaagcagaa aacttacaca gaggggctca tcattataca
     2701 gggcttttaa aactgcaaga acgagtgctc aataatgttg tcatccattt gcttggagat
     2761 gaagacccca gggtgcgaca tgttgccgca gcatcactaa ttaggcttgt cccaaagctg
     2821 ttttataaat gtgaccaagg acaagctgat ccagtagtgg ccgtggcaag agatcaaagc
     2881 agtgtttacc tgaaacttct catgcatgag acgcagcctc catctcattt ctccgtcagc
     2941 acaataacca gaatatatag aggctataac ctactaccaa gcataacaga cgtcactatg
     3001 gaaaataacc tttcaagagt tattgcagca gtttctcatg aactaatcac atcaaccacc
     3061 agagcactca catttggatg ctgtgaagct ttgtgtcttc tttccactgc cttcccagtt
     3121 tgcatttgga gtttaggttg gcactgtgga gtgcctccac tgagtgcctc agatgagtct
     3181 aggaagagct gtaccgttgg gatggccaca atgattctga ccctgctctc gtcagcttgg
     3241 ttcccattgg atctctcagc ccatcaagat gctttgattt tggccggaaa cttgcttgca
     3301 gccagtgctc ccaaatctct gagaagttca tgggcctctg aagaagaagc caacccagca
     3361 gccaccaagc aagaggaggt ctggccagcc ctgggggacc gggccctggt gcccatggtg
     3421 gagcagctct tctctcacct gctgaaggtg attaacattt gtgcccacgt cctggatgac
     3481 gtggctcctg gacccgcaat aaaggcagcc ttgccttctc taacaaaccc cccttctcta
     3541 agtcccatcc gacgaaaggg gaaggagaaa gaaccaggag aacaagcatc tgtaccgttg
     3601 agtcccaaga aaggcagtga ggccagtgca gcttctagac aatctgatac ctcaggtcct
     3661 gttacaacaa gtaaatcctc atcactgggg agtttctatc atcttccttc atacctcaaa
     3721 ctgcatgatg tcctgaaagc tacacacgct aactacaagg tcacgctgga tcttcagaac
     3781 agcacggaaa agtttggagg gtttcttcgc tcagccttgg atgttctttc tcagatacta
     3841 gagctggcca cactgcagga cattgggaag tgtgttgaag agatcctagg atacctgaaa
     3901 tcctgcttta gtcgagaacc aatgatggca actgtttgtg ttcaacaatt gttgaagact
     3961 ctctttggca caaacttggc ctcccagttt gatggcttat cttccaaccc cagcaagtca
     4021 caaggccgag cacagcgcct tggctcctcc agtgtgaggc caggcttgta ccactactgc
     4081 ttcatggccc cgtacaccca cttcacccag gccctcgctg acgccagcct gaggaacatg
     4141 gtgcaggcgg agcaggagaa cgacacctcg ggatggtttg atgtcctcca gaaagtgtct
     4201 acccagttga agacaaacct cacgagtgtc acaaagaacc gtgcagataa gaatgctatt
     4261 cataatcaca ttcgtttgtt tgaacctctt gttataaaag ctttaaaaca gtacacgact
     4321 acaacatgtg tgcagttaca gaagcaggtt ttagatttgc tggcgcagct ggttcagtta
     4381 cgggttaatt actgtcttct ggattcagat caggtgttta ttggctttgt attgaaacag
     4441 tttgaataca ttgaagtggg ccagttcagg gaatcagagg caatcattcc aaacatcttt
     4501 ttcttcttgg tattactatc ttatgaacgc tatcattcaa aacagatcat tggaattcct
     4561 aaaatcattc agctctgtga tggcatcatg gccagtggaa ggaaggctgt gacacatgcc
     4621 ataccggctc tgcagcccat agtccacgac ctctttgtat taagaggaac aaataaagct
     4681 gatgcaggaa aagagcttga aacccaaaaa gaggtggtgg tgtcaatgtt actgagactc
     4741 atccagtacc atcaggtgtt ggagatgttc attcttgtcc tgcagcagtg ccacaaggag
     4801 aatgaagaca agtggaagcg actgtctcga cagatagctg acatcatcct cccaatgtta
     4861 gccaaacagc agatgcacat tgactctcat gaagcccttg gagtgttaaa tacattattt
     4921 gagattttgg ccccttcctc cctccgtccg gtagacatgc ttttacggag tatgttcgtc
     4981 actccaaaca caatggcgtc cgtgagcact gttcaactgt ggatatcggg aattctggcc
     5041 attttgaggg ttctgatttc ccagtcaact gaagatattg ttctttctcg tattcaggag
     5101 ctctccttct ctccgtattt aatctcctgt acagtaatta ataggttaag agatggggac
     5161 agtacttcaa cgctagaaga acacagtgaa gggaaacaaa taaagaattt gccagaagaa
     5221 acattttcaa ggtttctatt acaactggtt ggtattcttt tagaagacat tgttacaaaa
     5281 cagctgaagg tggaaatgag tgagcagcaa catactttct attgccagga actaggcaca
     5341 ctgctaatgt gtctgatcca catcttcaag tctggaatgt tccggagaat cacagcagct
     5401 gccactaggc tgttccgcag tgatggctgt ggcggcagtt tctacaccct ggacagcttg
     5461 aacttgcggg ctcgttccat gatcaccacc cacccggccc tggtgctgct ctggtgtcag
     5521 atactgctgc ttgtcaacca caccgactac cgctggtggg cagaagtgca gcagaccccg
     5581 aaaagacaca gtctgtccag cacaaagtta cttagtcccc agatgtctgg agaagaggag
     5641 gattctgact tggcagccaa acttggaatg tgcaatagag aaatagtacg aagaggggct
     5701 ctcattctct tctgtgatta tgtctgtcag aacctccatg actccgagca cttaacgtgg
     5761 ctcattgtaa atcacattca agatctgatc agcctttccc acgagcctcc agtacaggac
     5821 ttcatcagtg ccgttcatcg gaactctgct gccagcggcc tgttcatcca ggcaattcag
     5881 tctcgttgtg aaaacctttc aactccaacc atgctgaaga aaactcttca gtgcttggag
     5941 gggatccatc tcagccagtc gggagctgtg ctcacgctgt atgtggacag gcttctgtgc
     6001 acccctttcc gtgtgctggc tcgcatggtc gacatccttg cttgtcgccg ggtagaaatg
     6061 cttctggctg caaatttaca gagcagcatg gcccagttgc caatggaaga actcaacaga
     6121 atccaggaat accttcagag cagcgggctc gctcagagac accaaaggct ctattccctg
     6181 ctggacaggt ttcgtctctc caccatgcaa gactcactta gtccctctcc tccagtctct
     6241 tcccacccgc tggacgggga tgggcacgtg tcactggaaa cagtgagtcc ggacaaagac
     6301 tggtacgttc atcttgtcaa atcccagtgt tggaccaggt cagattctgc actgctggaa
     6361 ggtgcagagc tggtgaatcg gattcctgct gaagatatga atgccttcat gatgaactcg
     6421 gagttcaacc taagcctgct agctccatgc ttaagcctag ggatgagtga aatttctggt
     6481 ggccagaaga gtgccctttt tgaagcagcc cgtgaggtga ctctggcccg tgtgagcggc
     6541 accgtgcagc agctccctgc tgtccatcat gtcttccagc ccgagctgcc tgcagaaccg
     6601 gcggcctact ggagcaagtt gaatgatctg tttggggatg ctgcactgta tcagtccctg
     6661 cccactctgg cccgggccct ggcacagtac ctggtggtgg tctccaaact gcccagtcat
     6721 ttgcaccttc ctcctgagaa agagaaggac attgtgaaat tcgtggtggc aacccttgag
     6781 gccctgtcct ggcatttgat ccatgagcag atcccgctga gtctggatct ccaggcaggg
     6841 ctggactgct gctgcctggc cctgcagctg cctggcctct ggagcgtggt ctcctccaca
     6901 gagtttgtga cccacgcctg ctccctcatc cactgtgtgc acttcatcct ggaggccgtt
     6961 gcagtgcagc ctggagagca gcttcttagt ccagaaagaa ggacaaatac cccaaaagcc
     7021 atcagcgagg aggaggagga agtagatcca aacacacaga atcctaagta tatcactgca
     7081 gcctgtgaga tggtggcaga aatggtggag tctctgcagt cggtgttggc cttgggtcat
     7141 aaaaggaata gcggcgtgcc ggcgtttctc acgccattgc tcaggaacat catcatcagc
     7201 ctggcccgcc tgccccttgt caacagctac acacgtgtgc ccccactggt gtggaagctt
     7261 ggatggtcac ccaaaccggg aggggatttt ggcacagcat tccctgagat ccccgtggag
     7321 ttcctccagg aaaaggaagt ctttaaggag ttcatctacc gcatcaacac actaggctgg
     7381 accagtcgta ctcagtttga agaaacttgg gccaccctcc ttggtgtcct ggtgacgcag
     7441 cccctcgtga tggagcagga ggagagccca ccagaagaag acacagagag gacccagatc
     7501 aacgtcctgg ccgtgcaggc catcacctca ctggtgctca gtgcaatgac tgtgcctgtg
     7561 gccggcaacc cagctgtaag ctgcttggag cagcagcccc ggaacaagcc tctgaaagct
     7621 ctcgacacca ggtttgggag gaagctgagc attatcagag ggattgtgga gcaagagatt
     7681 caagcaatgg tttcaaagag agagaatatt gccacccatc atttatatca ggcatgggat
     7741 cctgtccctt ctctgtctcc ggctactaca ggtgccctca tcagccacga gaagctgctg
     7801 ctgcagatca accccgagcg ggagctgggg agcatgagct acaaactcgg ccaggtgtcc
     7861 atacactccg tgtggctagg gaacagcatc acacccctga gggaggagga atgggacgag
     7921 gaagaggagg aggaggccga cgcccctgca ccttcgtcac cacccacgtc tccagtcaac
     7981 tccaggaaac accgggctgg agttgacatc cactcctgtt cgcagttttt gcttgagttg
     8041 tacagccgct ggatcctgcc gtccagctca gccaggagga ccccggccat cctgatcagt
     8101 gaggtggtca gatcccttct agtggtctca gacttgttca ccgagcgcaa ccagtttgag
     8161 ctgatgtatg tgacgctgac agaactgcga agggtgcacc cttcagaaga cgagatcctc
     8221 gctcagtacc tggtgcctgc cacctgcaag gcagctgccg tccttgggat ggacaaggcc
     8281 gtggcggagc ctgtcagccg cctgctggag agcacgctca ggagcagcca cctgcccagc
     8341 agggttggag ccctgcacgg catcctctat gtgctggagt gcgacctgct ggacgacact
     8401 gccaagcagc tcatcccggt catcagcgac tatctcctct ccaacctgaa agggatcgcc
     8461 cactgcgtga acattcacag ccagcagcac gtactggtca tgtgtgccac tgcgttttac
     8521 ctcattgaga actatcctct ggacgtaggg ccggaatttt cagcatcaat aatacagatg
     8581 tgtggggtga tgctgtctgg aagtgaggag tccaccccct ccatcattta ccactgtgcc
     8641 ctcagaggcc tggagcgcct cctgctctct gagcagctct cccgcctgga tgcagaatcg
     8701 ctggtcaagc tgagtgtgga cagagtgaac gtgcacagcc cgcaccgggc catggcggct
     8761 ctgggcctga tgctcacctg catgtacaca ggaaaggaga aagtcagtcc gggtagaact
     8821 tcagacccta atcctgcagc ccccgacagc gagtcagtga ttgttgctat ggagcgggta
     8881 tctgttcttt ttgataggat caggaaaggc tttccttgtg aagccagagt ggtggccagg
     8941 atcctgcccc agtttctaga cgacttcttc ccaccccagg acatcatgaa caaagtcatc
     9001 ggagagtttc tgtccaacca gcagccatac ccccagttca tggccaccgt ggtgtataag
     9061 gtgtttcaga ctctgcacag caccgggcag tcgtccatgg tccgggactg ggtcatgctg
     9121 tccctctcca acttcacgca gagggccccg gtcgccatgg ccacgtggag cctctcctgc
     9181 ttctttgtca gcgcgtccac cagcccgtgg gtcgcggcga tcctcccaca tgtcatcagc
     9241 aggatgggca agctggagca ggtggacgtg aaccttttct gcctggtcgc cacagacttc
     9301 tacagacacc agatagagga ggagctcgac cgcagggcct tccagtctgt gcttgaggtg
     9361 gttgcagccc caggaagccc atatcaccgg ctgctgactt gtttacgaaa tgtccacaag
     9421 gtcaccacct gc
//
