LOCUS       sequence-209876-        9600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8918..8927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      241 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaacc gccaccgccg
      301 ccgccgccgc cgccgcctcc tcagcttcct cagccgccgc cgcaggcaca gccgctgctg
      361 cctcagccgc agccgccccc gccgccgccc ccgccgccac ccggcccggc tgtggctgag
      421 gagccgctgc accgaccaaa gaaagaactt tcagctacca agaaagaccg tgtgaatcat
      481 tgtctgacaa tatgtgaaaa catagtggca cagtctgtca gaaattctcc agaatttcag
      541 aaacttctgg gcatcgctat ggaacttttt ctgctgtgca gtgatgacgc agagtcagat
      601 gtcaggatgg tggctgacga atgcctcaac aaagttatca aagctttgat ggattctaat
      661 cttccaaggt tacagctcga gctctataag gaaattaaaa agaatggtgc ccctcggagt
      721 ttgcgtgctg ccctgtggag gtttgctgag ctggctcacc tggttcggcc tcagaaatgc
      781 aggccttacc tggtgaacct tctgccgtgc ctgactcgaa caagcaagag acccgaagaa
      841 tcagtccagg agaccttggc tgcagctgtt cccaaaatta tggcttcttt tggcaatttt
      901 gcaaatgaca atgaaattaa ggttttgtta aaggccttca tagcgaacct gaagtcaagc
      961 tcccccacca ttcggcggac agcggctgga tcagcagtga gcatctgcca gcactcaaga
     1021 aggacacaat atttctatag ttggctacta aatgtgctct taggcttact cgttcctgtc
     1081 gaggatgaac actccactct gctgattctt ggcgtgctgc tcaccctgag gtatttggtg
     1141 cccttgctgc agcagcaggt caaggacaca agcctgaaag gcagcttcgg agtgacaagg
     1201 aaagaaatgg aagtctctcc ttctgcagag cagcttgtcc aggtttatga actgacgtta
     1261 catcatacac agcaccaaga ccacaatgtt gtgaccggag ccctggagct gttgcagcag
     1321 ctcttcagaa cgcctccacc cgagcttctg caaaccctga ccgcagtcgg gggcattggg
     1381 cagctcaccg ctgctaagga ggagtctggt ggccgaagcc gtagtgggag tattgtggaa
     1441 cttatagctg gagggggttc ctcatgcagc cctgtccttt caagaaaaca aaaaggcaaa
     1501 gtgctcttag gagaagaaga agccttggag gatgactctg aatcgagatc ggatgtcagc
     1561 agctctgcct taacagcctc agtgaaggat gagatcagtg gagagctggc tgcttcttca
     1621 ggggtttcca ctccagggtc agcaggtcat gacatcatca cagaacagcc acggtcacag
     1681 cacacactgc aggcggactc agtggatctg gccagctgtg acttgacaag ctctgccact
     1741 gatggggatg aggaggatat cttgagccac agctccagcc aggtcagcgc cgtcccatct
     1801 gaccctgcca tggacctgaa tgatgggacc caggcctcgt cgcccatcag cgacagctcc
     1861 cagaccacca ccgaagggcc tgattcagct gttacccctt cagacagttc tgaaattgtg
     1921 ttagacggta ccgacaacca gtatttgggc ctgcagattg gacagcccca ggatgaagat
     1981 gaggaagcca caggtattct tcctgatgaa gcctcggagg ccttcaggaa ctcttccatg
     2041 gcccttcaac aggcacattt attgaaaaac atgagtcact gcaggcagcc ttctgacagc
     2101 agtgttgata aatttgtgtt gagagatgaa gctactgaac cgggtgatca agaaaacaag
     2161 ccttgccgca tcaaaggtga cattggacag tccactgatg atgactctgc acctcttgtc
     2221 cattgtgtcc gccttttatc tgcttcgttt ttgctaacag ggggaaaaaa tgtgctggtt
     2281 ccggacaggg atgtgagggt cagcgtgaag gccctggccc tcagctgtgt gggagcagct
     2341 gtggccctcc acccggaatc tttcttcagc aaactctata aagttcctct tgacaccacg
     2401 gaataccctg aggaacagta tgtctcagac atcttgaact acatcgatca tggagaccca
     2461 caggttcgag gagccactgc cattctctgt gggaccctca tctgctccat cctcagcagg
     2521 tcccgcttcc acgtgggaga ttggatgggc accattagaa ccctcacagg aaatacattt
     2581 tctttggcgg attgcattcc tttgctgcgg aaaacactga aggatgagtc ttctgttact
     2641 tgcaagttag cttgtacagc tgtgaggaac tgtgtcatga gtctctgcag cagcagctac
     2701 agtgagttag gactgcagct gatcatcgat gtgctgactc tgaggaacag ttcctattgg
     2761 ctggtgagga cagagcttct ggaaaccctt gcagagattg acttcaggct ggtgagcttt
     2821 ttggaggcaa aagcagaaaa cttacacaga ggggctcatc attatacagg gcttttaaaa
     2881 ctgcaagaac gagtgctcaa taatgttgtc atccatttgc ttggagatga agaccccagg
     2941 gtgcgacatg ttgccgcagc atcactaatt aggcttgtcc caaagctgtt ttataaatgt
     3001 gaccaaggac aagctgatcc agtagtggcc gtggcaagag atcaaagcag tgtttacctg
     3061 aaacttctca tgcatgagac gcagcctcca tctcatttct ccgtcagcac aataaccaga
     3121 atatatagag gctataacct actaccaagc ataacagacg tcactatgga aaataacctt
     3181 tcaagagtta ttgcagcagt ttctcatgaa ctaatcacat caaccaccag agcactcaca
     3241 tttggatgct gtgaagcttt gtgtcttctt tccactgcct tcccagtttg catttggagt
     3301 ttaggttggc actgtggagt gcctccactg agtgcctcag atgagtctag gaagagctgt
     3361 accgttggga tggccacaat gattctgacc ctgctctcgt cagcttggtt cccattggat
     3421 ctctcagccc atcaagatgc tttgattttg gccggaaact tgcttgcagc cagtgctccc
     3481 aaatctctga gaagttcatg ggcctctgaa gaagaagcca acccagcagc caccaagcaa
     3541 gaggaggtct ggccagccct gggggaccgg gccctggtgc ccatggtgga gcagctcttc
     3601 tctcacctgc tgaaggtgat taacatttgt gcccacgtcc tggatgacgt ggctcctgga
     3661 cccgcaataa aggcagcctt gccttctcta acaaaccccc cttctctaag tcccatccga
     3721 cgaaagggga aggagaaaga accaggagaa caagcatctg taccgttgag tcccaagaaa
     3781 ggcagtgagg ccagtgcagc ttctagacaa tctgatacct caggtcctgt tacaacaagt
     3841 aaatcctcat cactggggag tttctatcat cttccttcat acctcaaact gcatgatgtc
     3901 ctgaaagcta cacacgctaa ctacaaggtc acgctggatc ttcagaacag cacggaaaag
     3961 tttggagggt ttcttcgctc agccttggat gttctttctc agatactaga gctggccaca
     4021 ctgcaggaca ttgggaagtg tgttgaagag atcctaggat acctgaaatc ctgctttagt
     4081 cgagaaccaa tgatggcaac tgtttgtgtt caacaattgt tgaagactct ctttggcaca
     4141 aacttggcct cccagtttga tggcttatct tccaacccca gcaagtcaca aggccgagca
     4201 cagcgccttg gctcctccag tgtgaggcca ggcttgtacc actactgctt catggccccg
     4261 tacacccact tcacccaggc cctcgctgac gccagcctga ggaacatggt gcaggcggag
     4321 caggagaacg acacctcggg atggtttgat gtcctccaga aagtgtctac ccagttgaag
     4381 acaaacctca cgagtgtcac aaagaaccgt gcagataaga atgctattca taatcacatt
     4441 cgtttgtttg aacctcttgt tataaaagct ttaaaacagt acacgactac aacatgtgtg
     4501 cagttacaga agcaggtttt agatttgctg gcgcagctgg ttcagttacg ggttaattac
     4561 tgtcttctgg attcagatca ggtgtttatt ggctttgtat tgaaacagtt tgaatacatt
     4621 gaagtgggcc agttcaggga atcagaggca atcattccaa acatcttttt cttcttggta
     4681 ttactatctt atgaacgcta tcattcaaaa cagatcattg gaattcctaa aatcattcag
     4741 ctctgtgatg gcatcatggc cagtggaagg aaggctgtga cacatgccat accggctctg
     4801 cagcccatag tccacgacct ctttgtatta agaggaacaa ataaagctga tgcaggaaaa
     4861 gagcttgaaa cccaaaaaga ggtggtggtg tcaatgttac tgagactcat ccagtaccat
     4921 caggtgttgg agatgttcat tcttgtcctg cagcagtgcc acaaggagaa tgaagacaag
     4981 tggaagcgac tgtctcgaca gatagctgac atcatcctcc caatgttagc caaacagcag
     5041 atgcacattg actctcatga agcccttgga gtgttaaata cattatttga gattttggcc
     5101 ccttcctccc tccgtccggt agacatgctt ttacggagta tgttcgtcac tccaaacaca
     5161 atggcgtccg tgagcactgt tcaactgtgg atatcgggaa ttctggccat tttgagggtt
     5221 ctgatttccc agtcaactga agatattgtt ctttctcgta ttcaggagct ctccttctct
     5281 ccgtatttaa tctcctgtac agtaattaat aggttaagag atggggacag tacttcaacg
     5341 ctagaagaac acagtgaagg gaaacaaata aagaatttgc cagaagaaac attttcaagg
     5401 tttctattac aactggttgg tattctttta gaagacattg ttacaaaaca gctgaaggtg
     5461 gaaatgagtg agcagcaaca tactttctat tgccaggaac taggcacact gctaatgtgt
     5521 ctgatccaca tcttcaagtc tggaatgttc cggagaatca cagcagctgc cactaggctg
     5581 ttccgcagtg atggctgtgg cggcagtttc tacaccctgg acagcttgaa cttgcgggct
     5641 cgttccatga tcaccaccca cccggccctg gtgctgctct ggtgtcagat actgctgctt
     5701 gtcaaccaca ccgactaccg ctggtgggca gaagtgcagc agaccccgaa aagacacagt
     5761 ctgtccagca caaagttact tagtccccag atgtctggag aagaggagga ttctgacttg
     5821 gcagccaaac ttggaatgtg caatagagaa atagtacgaa gaggggctct cattctcttc
     5881 tgtgattatg tctgtcagaa cctccatgac tccgagcact taacgtggct cattgtaaat
     5941 cacattcaag atctgatcag cctttcccac gagcctccag tacaggactt catcagtgcc
     6001 gttcatcgga actctgctgc cagcggcctg ttcatccagg caattcagtc tcgttgtgaa
     6061 aacctttcaa ctccaaccat gctgaagaaa actcttcagt gcttggaggg gatccatctc
     6121 agccagtcgg gagctgtgct cacgctgtat gtggacaggc ttctgtgcac ccctttccgt
     6181 gtgctggctc gcatggtcga catccttgct tgtcgccggg tagaaatgct tctggctgca
     6241 aatttacaga gcagcatggc ccagttgcca atggaagaac tcaacagaat ccaggaatac
     6301 cttcagagca gcgggctcgc tcagagacac caaaggctct attccctgct ggacaggttt
     6361 cgtctctcca ccatgcaaga ctcacttagt ccctctcctc cagtctcttc ccacccgctg
     6421 gacggggatg ggcacgtgtc actggaaaca gtgagtccgg acaaagactg gtacgttcat
     6481 cttgtcaaat cccagtgttg gaccaggtca gattctgcac tgctggaagg tgcagagctg
     6541 gtgaatcgga ttcctgctga agatatgaat gccttcatga tgaactcgga gttcaaccta
     6601 agcctgctag ctccatgctt aagcctaggg atgagtgaaa tttctggtgg ccagaagagt
     6661 gccctttttg aagcagcccg tgaggtgact ctggcccgtg tgagcggcac cgtgcagcag
     6721 ctccctgctg tccatcatgt cttccagccc gagctgcctg cagaaccggc ggcctactgg
     6781 agcaagttga atgatctgtt tggggatgct gcactgtatc agtccctgcc cactctggcc
     6841 cgggccctgg cacagtacct ggtggtggtc tccaaactgc ccagtcattt gcaccttcct
     6901 cctgagaaag agaaggacat tgtgaaattc gtggtggcaa cccttgaggc cctgtcctgg
     6961 catttgatcc atgagcagat cccgctgagt ctggatctcc aggcagggct ggactgctgc
     7021 tgcctggccc tgcagctgcc tggcctctgg agcgtggtct cctccacaga gtttgtgacc
     7081 cacgcctgct ccctcatcca ctgtgtgcac ttcatcctgg aggccgttgc agtgcagcct
     7141 ggagagcagc ttcttagtcc agaaagaagg acaaataccc caaaagccat cagcgaggag
     7201 gaggaggaag tagatccaaa cacacagaat cctaagtata tcactgcagc ctgtgagatg
     7261 gtggcagaaa tggtggagtc tctgcagtcg gtgttggcct tgggtcataa aaggaatagc
     7321 ggcgtgccgg cgtttctcac gccattgctc aggaacatca tcatcagcct ggcccgcctg
     7381 ccccttgtca acagctacac acgtgtgccc ccactggtgt ggaagcttgg atggtcaccc
     7441 aaaccgggag gggattttgg cacagcattc cctgagatcc ccgtggagtt cctccaggaa
     7501 aaggaagtct ttaaggagtt catctaccgc atcaacacac taggctggac cagtcgtact
     7561 cagtttgaag aaacttgggc caccctcctt ggtgtcctgg tgacgcagcc cctcgtgatg
     7621 gagcaggagg agagcccacc agaagaagac acagagagga cccagatcaa cgtcctggcc
     7681 gtgcaggcca tcacctcact ggtgctcagt gcaatgactg tgcctgtggc cggcaaccca
     7741 gctgtaagct gcttggagca gcagccccgg aacaagcctc tgaaagctct cgacaccagg
     7801 tttgggagga agctgagcat tatcagaggg attgtggagc aagagattca agcaatggtt
     7861 tcaaagagag agaatattgc cacccatcat ttatatcagg catgggatcc tgtcccttct
     7921 ctgtctccgg ctactacagg tgccctcatc agccacgaga agctgctgct gcagatcaac
     7981 cccgagcggg agctggggag catgagctac aaactcggcc aggtgtccat acactccgtg
     8041 tggctaggga acagcatcac acccctgagg gaggaggaat gggacgagga agaggaggag
     8101 gaggccgacg cccctgcacc ttcgtcacca cccacgtctc cagtcaactc caggaaacac
     8161 cgggctggag ttgacatcca ctcctgttcg cagtttttgc ttgagttgta cagccgctgg
     8221 atcctgccgt ccagctcagc caggaggacc ccggccatcc tgatcagtga ggtggtcaga
     8281 tcccttctag tggtctcaga cttgttcacc gagcgcaacc agtttgagct gatgtatgtg
     8341 acgctgacag aactgcgaag ggtgcaccct tcagaagacg agatcctcgc tcagtacctg
     8401 gtgcctgcca cctgcaaggc agctgccgtc cttgggatgg acaaggccgt ggcggagcct
     8461 gtcagccgcc tgctggagag cacgctcagg agcagccacc tgcccagcag ggttggagcc
     8521 ctgcacggca tcctctatgt gctggagtgc gacctgctgg acgacactgc caagcagctc
     8581 atcccggtca tcagcgacta tctcctctcc aacctgaaag ggatcgccca ctgcgtgaac
     8641 attcacagcc agcagcacgt actggtcatg tgtgccactg cgttttacct cattgagaac
     8701 tatcctctgg acgtagggcc ggaattttca gcatcaataa tacagatgtg tggggtgatg
     8761 ctgtctggaa gtgaggagtc caccccctcc atcatttacc actgtgccct cagaggcctg
     8821 gagcgcctcc tgctctctga gcagctctcc cgcctggatg cagaatcgct ggtcaagctg
     8881 agtgtggaca gagtgaacgt gcacagcccg caccgggcca tggcggctct gggcctgatg
     8941 ctcacctgca tgtacacagg aaaggagaaa gtcagtccgg gtagaacttc agaccctaat
     9001 cctgcagccc ccgacagcga gtcagtgatt gttgctatgg agcgggtatc tgttcttttt
     9061 gataggatca ggaaaggctt tccttgtgaa gccagagtgg tggccaggat cctgccccag
     9121 tttctagacg acttcttccc accccaggac atcatgaaca aagtcatcgg agagtttctg
     9181 tccaaccagc agccataccc ccagttcatg gccaccgtgg tgtataaggt gtttcagact
     9241 ctgcacagca ccgggcagtc gtccatggtc cgggactggg tcatgctgtc cctctccaac
     9301 ttcacgcaga gggccccggt cgccatggcc acgtggagcc tctcctgctt ctttgtcagc
     9361 gcgtccacca gcccgtgggt cgcggcgatc ctcccacatg tcatcagcag gatgggcaag
     9421 ctggagcagg tggacgtgaa ccttttctgc ctggtcgcca cagacttcta cagacaccag
     9481 atagaggagg agctcgaccg cagggccttc cagtctgtgc ttgaggtggt tgcagcccca
     9541 ggaagcccat atcaccggct gctgacttgt ttacgaaatg tccacaaggt caccacctgc
//
