LOCUS       sequence-209878-        9525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8843..8852
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caaccgccac cgccgccgcc gccgccgccg
      241 cctcctcagc ttcctcagcc gccgccgcag gcacagccgc tgctgcctca gccgcagccg
      301 cccccgccgc cgcccccgcc gccacccggc ccggctgtgg ctgaggagcc gctgcaccga
      361 ccaaagaaag aactttcagc taccaagaaa gaccgtgtga atcattgtct gacaatatgt
      421 gaaaacatag tggcacagtc tgtcagaaat tctccagaat ttcagaaact tctgggcatc
      481 gctatggaac tttttctgct gtgcagtgat gacgcagagt cagatgtcag gatggtggct
      541 gacgaatgcc tcaacaaagt tatcaaagct ttgatggatt ctaatcttcc aaggttacag
      601 ctcgagctct ataaggaaat taaaaagaat ggtgcccctc ggagtttgcg tgctgccctg
      661 tggaggtttg ctgagctggc tcacctggtt cggcctcaga aatgcaggcc ttacctggtg
      721 aaccttctgc cgtgcctgac tcgaacaagc aagagacccg aagaatcagt ccaggagacc
      781 ttggctgcag ctgttcccaa aattatggct tcttttggca attttgcaaa tgacaatgaa
      841 attaaggttt tgttaaaggc cttcatagcg aacctgaagt caagctcccc caccattcgg
      901 cggacagcgg ctggatcagc agtgagcatc tgccagcact caagaaggac acaatatttc
      961 tatagttggc tactaaatgt gctcttaggc ttactcgttc ctgtcgagga tgaacactcc
     1021 actctgctga ttcttggcgt gctgctcacc ctgaggtatt tggtgccctt gctgcagcag
     1081 caggtcaagg acacaagcct gaaaggcagc ttcggagtga caaggaaaga aatggaagtc
     1141 tctccttctg cagagcagct tgtccaggtt tatgaactga cgttacatca tacacagcac
     1201 caagaccaca atgttgtgac cggagccctg gagctgttgc agcagctctt cagaacgcct
     1261 ccacccgagc ttctgcaaac cctgaccgca gtcgggggca ttgggcagct caccgctgct
     1321 aaggaggagt ctggtggccg aagccgtagt gggagtattg tggaacttat agctggaggg
     1381 ggttcctcat gcagccctgt cctttcaaga aaacaaaaag gcaaagtgct cttaggagaa
     1441 gaagaagcct tggaggatga ctctgaatcg agatcggatg tcagcagctc tgccttaaca
     1501 gcctcagtga aggatgagat cagtggagag ctggctgctt cttcaggggt ttccactcca
     1561 gggtcagcag gtcatgacat catcacagaa cagccacggt cacagcacac actgcaggcg
     1621 gactcagtgg atctggccag ctgtgacttg acaagctctg ccactgatgg ggatgaggag
     1681 gatatcttga gccacagctc cagccaggtc agcgccgtcc catctgaccc tgccatggac
     1741 ctgaatgatg ggacccaggc ctcgtcgccc atcagcgaca gctcccagac caccaccgaa
     1801 gggcctgatt cagctgttac cccttcagac agttctgaaa ttgtgttaga cggtaccgac
     1861 aaccagtatt tgggcctgca gattggacag ccccaggatg aagatgagga agccacaggt
     1921 attcttcctg atgaagcctc ggaggccttc aggaactctt ccatggccct tcaacaggca
     1981 catttattga aaaacatgag tcactgcagg cagccttctg acagcagtgt tgataaattt
     2041 gtgttgagag atgaagctac tgaaccgggt gatcaagaaa acaagccttg ccgcatcaaa
     2101 ggtgacattg gacagtccac tgatgatgac tctgcacctc ttgtccattg tgtccgcctt
     2161 ttatctgctt cgtttttgct aacaggggga aaaaatgtgc tggttccgga cagggatgtg
     2221 agggtcagcg tgaaggccct ggccctcagc tgtgtgggag cagctgtggc cctccacccg
     2281 gaatctttct tcagcaaact ctataaagtt cctcttgaca ccacggaata ccctgaggaa
     2341 cagtatgtct cagacatctt gaactacatc gatcatggag acccacaggt tcgaggagcc
     2401 actgccattc tctgtgggac cctcatctgc tccatcctca gcaggtcccg cttccacgtg
     2461 ggagattgga tgggcaccat tagaaccctc acaggaaata cattttcttt ggcggattgc
     2521 attcctttgc tgcggaaaac actgaaggat gagtcttctg ttacttgcaa gttagcttgt
     2581 acagctgtga ggaactgtgt catgagtctc tgcagcagca gctacagtga gttaggactg
     2641 cagctgatca tcgatgtgct gactctgagg aacagttcct attggctggt gaggacagag
     2701 cttctggaaa cccttgcaga gattgacttc aggctggtga gctttttgga ggcaaaagca
     2761 gaaaacttac acagaggggc tcatcattat acagggcttt taaaactgca agaacgagtg
     2821 ctcaataatg ttgtcatcca tttgcttgga gatgaagacc ccagggtgcg acatgttgcc
     2881 gcagcatcac taattaggct tgtcccaaag ctgttttata aatgtgacca aggacaagct
     2941 gatccagtag tggccgtggc aagagatcaa agcagtgttt acctgaaact tctcatgcat
     3001 gagacgcagc ctccatctca tttctccgtc agcacaataa ccagaatata tagaggctat
     3061 aacctactac caagcataac agacgtcact atggaaaata acctttcaag agttattgca
     3121 gcagtttctc atgaactaat cacatcaacc accagagcac tcacatttgg atgctgtgaa
     3181 gctttgtgtc ttctttccac tgccttccca gtttgcattt ggagtttagg ttggcactgt
     3241 ggagtgcctc cactgagtgc ctcagatgag tctaggaaga gctgtaccgt tgggatggcc
     3301 acaatgattc tgaccctgct ctcgtcagct tggttcccat tggatctctc agcccatcaa
     3361 gatgctttga ttttggccgg aaacttgctt gcagccagtg ctcccaaatc tctgagaagt
     3421 tcatgggcct ctgaagaaga agccaaccca gcagccacca agcaagagga ggtctggcca
     3481 gccctggggg accgggccct ggtgcccatg gtggagcagc tcttctctca cctgctgaag
     3541 gtgattaaca tttgtgccca cgtcctggat gacgtggctc ctggacccgc aataaaggca
     3601 gccttgcctt ctctaacaaa ccccccttct ctaagtccca tccgacgaaa ggggaaggag
     3661 aaagaaccag gagaacaagc atctgtaccg ttgagtccca agaaaggcag tgaggccagt
     3721 gcagcttcta gacaatctga tacctcaggt cctgttacaa caagtaaatc ctcatcactg
     3781 gggagtttct atcatcttcc ttcatacctc aaactgcatg atgtcctgaa agctacacac
     3841 gctaactaca aggtcacgct ggatcttcag aacagcacgg aaaagtttgg agggtttctt
     3901 cgctcagcct tggatgttct ttctcagata ctagagctgg ccacactgca ggacattggg
     3961 aagtgtgttg aagagatcct aggatacctg aaatcctgct ttagtcgaga accaatgatg
     4021 gcaactgttt gtgttcaaca attgttgaag actctctttg gcacaaactt ggcctcccag
     4081 tttgatggct tatcttccaa ccccagcaag tcacaaggcc gagcacagcg ccttggctcc
     4141 tccagtgtga ggccaggctt gtaccactac tgcttcatgg ccccgtacac ccacttcacc
     4201 caggccctcg ctgacgccag cctgaggaac atggtgcagg cggagcagga gaacgacacc
     4261 tcgggatggt ttgatgtcct ccagaaagtg tctacccagt tgaagacaaa cctcacgagt
     4321 gtcacaaaga accgtgcaga taagaatgct attcataatc acattcgttt gtttgaacct
     4381 cttgttataa aagctttaaa acagtacacg actacaacat gtgtgcagtt acagaagcag
     4441 gttttagatt tgctggcgca gctggttcag ttacgggtta attactgtct tctggattca
     4501 gatcaggtgt ttattggctt tgtattgaaa cagtttgaat acattgaagt gggccagttc
     4561 agggaatcag aggcaatcat tccaaacatc tttttcttct tggtattact atcttatgaa
     4621 cgctatcatt caaaacagat cattggaatt cctaaaatca ttcagctctg tgatggcatc
     4681 atggccagtg gaaggaaggc tgtgacacat gccataccgg ctctgcagcc catagtccac
     4741 gacctctttg tattaagagg aacaaataaa gctgatgcag gaaaagagct tgaaacccaa
     4801 aaagaggtgg tggtgtcaat gttactgaga ctcatccagt accatcaggt gttggagatg
     4861 ttcattcttg tcctgcagca gtgccacaag gagaatgaag acaagtggaa gcgactgtct
     4921 cgacagatag ctgacatcat cctcccaatg ttagccaaac agcagatgca cattgactct
     4981 catgaagccc ttggagtgtt aaatacatta tttgagattt tggccccttc ctccctccgt
     5041 ccggtagaca tgcttttacg gagtatgttc gtcactccaa acacaatggc gtccgtgagc
     5101 actgttcaac tgtggatatc gggaattctg gccattttga gggttctgat ttcccagtca
     5161 actgaagata ttgttctttc tcgtattcag gagctctcct tctctccgta tttaatctcc
     5221 tgtacagtaa ttaataggtt aagagatggg gacagtactt caacgctaga agaacacagt
     5281 gaagggaaac aaataaagaa tttgccagaa gaaacatttt caaggtttct attacaactg
     5341 gttggtattc ttttagaaga cattgttaca aaacagctga aggtggaaat gagtgagcag
     5401 caacatactt tctattgcca ggaactaggc acactgctaa tgtgtctgat ccacatcttc
     5461 aagtctggaa tgttccggag aatcacagca gctgccacta ggctgttccg cagtgatggc
     5521 tgtggcggca gtttctacac cctggacagc ttgaacttgc gggctcgttc catgatcacc
     5581 acccacccgg ccctggtgct gctctggtgt cagatactgc tgcttgtcaa ccacaccgac
     5641 taccgctggt gggcagaagt gcagcagacc ccgaaaagac acagtctgtc cagcacaaag
     5701 ttacttagtc cccagatgtc tggagaagag gaggattctg acttggcagc caaacttgga
     5761 atgtgcaata gagaaatagt acgaagaggg gctctcattc tcttctgtga ttatgtctgt
     5821 cagaacctcc atgactccga gcacttaacg tggctcattg taaatcacat tcaagatctg
     5881 atcagccttt cccacgagcc tccagtacag gacttcatca gtgccgttca tcggaactct
     5941 gctgccagcg gcctgttcat ccaggcaatt cagtctcgtt gtgaaaacct ttcaactcca
     6001 accatgctga agaaaactct tcagtgcttg gaggggatcc atctcagcca gtcgggagct
     6061 gtgctcacgc tgtatgtgga caggcttctg tgcacccctt tccgtgtgct ggctcgcatg
     6121 gtcgacatcc ttgcttgtcg ccgggtagaa atgcttctgg ctgcaaattt acagagcagc
     6181 atggcccagt tgccaatgga agaactcaac agaatccagg aataccttca gagcagcggg
     6241 ctcgctcaga gacaccaaag gctctattcc ctgctggaca ggtttcgtct ctccaccatg
     6301 caagactcac ttagtccctc tcctccagtc tcttcccacc cgctggacgg ggatgggcac
     6361 gtgtcactgg aaacagtgag tccggacaaa gactggtacg ttcatcttgt caaatcccag
     6421 tgttggacca ggtcagattc tgcactgctg gaaggtgcag agctggtgaa tcggattcct
     6481 gctgaagata tgaatgcctt catgatgaac tcggagttca acctaagcct gctagctcca
     6541 tgcttaagcc tagggatgag tgaaatttct ggtggccaga agagtgccct ttttgaagca
     6601 gcccgtgagg tgactctggc ccgtgtgagc ggcaccgtgc agcagctccc tgctgtccat
     6661 catgtcttcc agcccgagct gcctgcagaa ccggcggcct actggagcaa gttgaatgat
     6721 ctgtttgggg atgctgcact gtatcagtcc ctgcccactc tggcccgggc cctggcacag
     6781 tacctggtgg tggtctccaa actgcccagt catttgcacc ttcctcctga gaaagagaag
     6841 gacattgtga aattcgtggt ggcaaccctt gaggccctgt cctggcattt gatccatgag
     6901 cagatcccgc tgagtctgga tctccaggca gggctggact gctgctgcct ggccctgcag
     6961 ctgcctggcc tctggagcgt ggtctcctcc acagagtttg tgacccacgc ctgctccctc
     7021 atccactgtg tgcacttcat cctggaggcc gttgcagtgc agcctggaga gcagcttctt
     7081 agtccagaaa gaaggacaaa taccccaaaa gccatcagcg aggaggagga ggaagtagat
     7141 ccaaacacac agaatcctaa gtatatcact gcagcctgtg agatggtggc agaaatggtg
     7201 gagtctctgc agtcggtgtt ggccttgggt cataaaagga atagcggcgt gccggcgttt
     7261 ctcacgccat tgctcaggaa catcatcatc agcctggccc gcctgcccct tgtcaacagc
     7321 tacacacgtg tgcccccact ggtgtggaag cttggatggt cacccaaacc gggaggggat
     7381 tttggcacag cattccctga gatccccgtg gagttcctcc aggaaaagga agtctttaag
     7441 gagttcatct accgcatcaa cacactaggc tggaccagtc gtactcagtt tgaagaaact
     7501 tgggccaccc tccttggtgt cctggtgacg cagcccctcg tgatggagca ggaggagagc
     7561 ccaccagaag aagacacaga gaggacccag atcaacgtcc tggccgtgca ggccatcacc
     7621 tcactggtgc tcagtgcaat gactgtgcct gtggccggca acccagctgt aagctgcttg
     7681 gagcagcagc cccggaacaa gcctctgaaa gctctcgaca ccaggtttgg gaggaagctg
     7741 agcattatca gagggattgt ggagcaagag attcaagcaa tggtttcaaa gagagagaat
     7801 attgccaccc atcatttata tcaggcatgg gatcctgtcc cttctctgtc tccggctact
     7861 acaggtgccc tcatcagcca cgagaagctg ctgctgcaga tcaaccccga gcgggagctg
     7921 gggagcatga gctacaaact cggccaggtg tccatacact ccgtgtggct agggaacagc
     7981 atcacacccc tgagggagga ggaatgggac gaggaagagg aggaggaggc cgacgcccct
     8041 gcaccttcgt caccacccac gtctccagtc aactccagga aacaccgggc tggagttgac
     8101 atccactcct gttcgcagtt tttgcttgag ttgtacagcc gctggatcct gccgtccagc
     8161 tcagccagga ggaccccggc catcctgatc agtgaggtgg tcagatccct tctagtggtc
     8221 tcagacttgt tcaccgagcg caaccagttt gagctgatgt atgtgacgct gacagaactg
     8281 cgaagggtgc acccttcaga agacgagatc ctcgctcagt acctggtgcc tgccacctgc
     8341 aaggcagctg ccgtccttgg gatggacaag gccgtggcgg agcctgtcag ccgcctgctg
     8401 gagagcacgc tcaggagcag ccacctgccc agcagggttg gagccctgca cggcatcctc
     8461 tatgtgctgg agtgcgacct gctggacgac actgccaagc agctcatccc ggtcatcagc
     8521 gactatctcc tctccaacct gaaagggatc gcccactgcg tgaacattca cagccagcag
     8581 cacgtactgg tcatgtgtgc cactgcgttt tacctcattg agaactatcc tctggacgta
     8641 gggccggaat tttcagcatc aataatacag atgtgtgggg tgatgctgtc tggaagtgag
     8701 gagtccaccc cctccatcat ttaccactgt gccctcagag gcctggagcg cctcctgctc
     8761 tctgagcagc tctcccgcct ggatgcagaa tcgctggtca agctgagtgt ggacagagtg
     8821 aacgtgcaca gcccgcaccg ggccatggcg gctctgggcc tgatgctcac ctgcatgtac
     8881 acaggaaagg agaaagtcag tccgggtaga acttcagacc ctaatcctgc agcccccgac
     8941 agcgagtcag tgattgttgc tatggagcgg gtatctgttc tttttgatag gatcaggaaa
     9001 ggctttcctt gtgaagccag agtggtggcc aggatcctgc cccagtttct agacgacttc
     9061 ttcccacccc aggacatcat gaacaaagtc atcggagagt ttctgtccaa ccagcagcca
     9121 tacccccagt tcatggccac cgtggtgtat aaggtgtttc agactctgca cagcaccggg
     9181 cagtcgtcca tggtccggga ctgggtcatg ctgtccctct ccaacttcac gcagagggcc
     9241 ccggtcgcca tggccacgtg gagcctctcc tgcttctttg tcagcgcgtc caccagcccg
     9301 tgggtcgcgg cgatcctccc acatgtcatc agcaggatgg gcaagctgga gcaggtggac
     9361 gtgaaccttt tctgcctggt cgccacagac ttctacagac accagataga ggaggagctc
     9421 gaccgcaggg ccttccagtc tgtgcttgag gtggttgcag ccccaggaag cccatatcac
     9481 cggctgctga cttgtttacg aaatgtccac aaggtcacca cctgc
//
