LOCUS       sequence-209879-        9582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8900..8909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      241 caacagcaac aacagcagca acagcaacaa ccgccaccgc cgccgccgcc gccgccgcct
      301 cctcagcttc ctcagccgcc gccgcaggca cagccgctgc tgcctcagcc gcagccgccc
      361 ccgccgccgc ccccgccgcc acccggcccg gctgtggctg aggagccgct gcaccgacca
      421 aagaaagaac tttcagctac caagaaagac cgtgtgaatc attgtctgac aatatgtgaa
      481 aacatagtgg cacagtctgt cagaaattct ccagaatttc agaaacttct gggcatcgct
      541 atggaacttt ttctgctgtg cagtgatgac gcagagtcag atgtcaggat ggtggctgac
      601 gaatgcctca acaaagttat caaagctttg atggattcta atcttccaag gttacagctc
      661 gagctctata aggaaattaa aaagaatggt gcccctcgga gtttgcgtgc tgccctgtgg
      721 aggtttgctg agctggctca cctggttcgg cctcagaaat gcaggcctta cctggtgaac
      781 cttctgccgt gcctgactcg aacaagcaag agacccgaag aatcagtcca ggagaccttg
      841 gctgcagctg ttcccaaaat tatggcttct tttggcaatt ttgcaaatga caatgaaatt
      901 aaggttttgt taaaggcctt catagcgaac ctgaagtcaa gctcccccac cattcggcgg
      961 acagcggctg gatcagcagt gagcatctgc cagcactcaa gaaggacaca atatttctat
     1021 agttggctac taaatgtgct cttaggctta ctcgttcctg tcgaggatga acactccact
     1081 ctgctgattc ttggcgtgct gctcaccctg aggtatttgg tgcccttgct gcagcagcag
     1141 gtcaaggaca caagcctgaa aggcagcttc ggagtgacaa ggaaagaaat ggaagtctct
     1201 ccttctgcag agcagcttgt ccaggtttat gaactgacgt tacatcatac acagcaccaa
     1261 gaccacaatg ttgtgaccgg agccctggag ctgttgcagc agctcttcag aacgcctcca
     1321 cccgagcttc tgcaaaccct gaccgcagtc gggggcattg ggcagctcac cgctgctaag
     1381 gaggagtctg gtggccgaag ccgtagtggg agtattgtgg aacttatagc tggagggggt
     1441 tcctcatgca gccctgtcct ttcaagaaaa caaaaaggca aagtgctctt aggagaagaa
     1501 gaagccttgg aggatgactc tgaatcgaga tcggatgtca gcagctctgc cttaacagcc
     1561 tcagtgaagg atgagatcag tggagagctg gctgcttctt caggggtttc cactccaggg
     1621 tcagcaggtc atgacatcat cacagaacag ccacggtcac agcacacact gcaggcggac
     1681 tcagtggatc tggccagctg tgacttgaca agctctgcca ctgatgggga tgaggaggat
     1741 atcttgagcc acagctccag ccaggtcagc gccgtcccat ctgaccctgc catggacctg
     1801 aatgatggga cccaggcctc gtcgcccatc agcgacagct cccagaccac caccgaaggg
     1861 cctgattcag ctgttacccc ttcagacagt tctgaaattg tgttagacgg taccgacaac
     1921 cagtatttgg gcctgcagat tggacagccc caggatgaag atgaggaagc cacaggtatt
     1981 cttcctgatg aagcctcgga ggccttcagg aactcttcca tggcccttca acaggcacat
     2041 ttattgaaaa acatgagtca ctgcaggcag ccttctgaca gcagtgttga taaatttgtg
     2101 ttgagagatg aagctactga accgggtgat caagaaaaca agccttgccg catcaaaggt
     2161 gacattggac agtccactga tgatgactct gcacctcttg tccattgtgt ccgcctttta
     2221 tctgcttcgt ttttgctaac agggggaaaa aatgtgctgg ttccggacag ggatgtgagg
     2281 gtcagcgtga aggccctggc cctcagctgt gtgggagcag ctgtggccct ccacccggaa
     2341 tctttcttca gcaaactcta taaagttcct cttgacacca cggaataccc tgaggaacag
     2401 tatgtctcag acatcttgaa ctacatcgat catggagacc cacaggttcg aggagccact
     2461 gccattctct gtgggaccct catctgctcc atcctcagca ggtcccgctt ccacgtggga
     2521 gattggatgg gcaccattag aaccctcaca ggaaatacat tttctttggc ggattgcatt
     2581 cctttgctgc ggaaaacact gaaggatgag tcttctgtta cttgcaagtt agcttgtaca
     2641 gctgtgagga actgtgtcat gagtctctgc agcagcagct acagtgagtt aggactgcag
     2701 ctgatcatcg atgtgctgac tctgaggaac agttcctatt ggctggtgag gacagagctt
     2761 ctggaaaccc ttgcagagat tgacttcagg ctggtgagct ttttggaggc aaaagcagaa
     2821 aacttacaca gaggggctca tcattataca gggcttttaa aactgcaaga acgagtgctc
     2881 aataatgttg tcatccattt gcttggagat gaagacccca gggtgcgaca tgttgccgca
     2941 gcatcactaa ttaggcttgt cccaaagctg ttttataaat gtgaccaagg acaagctgat
     3001 ccagtagtgg ccgtggcaag agatcaaagc agtgtttacc tgaaacttct catgcatgag
     3061 acgcagcctc catctcattt ctccgtcagc acaataacca gaatatatag aggctataac
     3121 ctactaccaa gcataacaga cgtcactatg gaaaataacc tttcaagagt tattgcagca
     3181 gtttctcatg aactaatcac atcaaccacc agagcactca catttggatg ctgtgaagct
     3241 ttgtgtcttc tttccactgc cttcccagtt tgcatttgga gtttaggttg gcactgtgga
     3301 gtgcctccac tgagtgcctc agatgagtct aggaagagct gtaccgttgg gatggccaca
     3361 atgattctga ccctgctctc gtcagcttgg ttcccattgg atctctcagc ccatcaagat
     3421 gctttgattt tggccggaaa cttgcttgca gccagtgctc ccaaatctct gagaagttca
     3481 tgggcctctg aagaagaagc caacccagca gccaccaagc aagaggaggt ctggccagcc
     3541 ctgggggacc gggccctggt gcccatggtg gagcagctct tctctcacct gctgaaggtg
     3601 attaacattt gtgcccacgt cctggatgac gtggctcctg gacccgcaat aaaggcagcc
     3661 ttgccttctc taacaaaccc cccttctcta agtcccatcc gacgaaaggg gaaggagaaa
     3721 gaaccaggag aacaagcatc tgtaccgttg agtcccaaga aaggcagtga ggccagtgca
     3781 gcttctagac aatctgatac ctcaggtcct gttacaacaa gtaaatcctc atcactgggg
     3841 agtttctatc atcttccttc atacctcaaa ctgcatgatg tcctgaaagc tacacacgct
     3901 aactacaagg tcacgctgga tcttcagaac agcacggaaa agtttggagg gtttcttcgc
     3961 tcagccttgg atgttctttc tcagatacta gagctggcca cactgcagga cattgggaag
     4021 tgtgttgaag agatcctagg atacctgaaa tcctgcttta gtcgagaacc aatgatggca
     4081 actgtttgtg ttcaacaatt gttgaagact ctctttggca caaacttggc ctcccagttt
     4141 gatggcttat cttccaaccc cagcaagtca caaggccgag cacagcgcct tggctcctcc
     4201 agtgtgaggc caggcttgta ccactactgc ttcatggccc cgtacaccca cttcacccag
     4261 gccctcgctg acgccagcct gaggaacatg gtgcaggcgg agcaggagaa cgacacctcg
     4321 ggatggtttg atgtcctcca gaaagtgtct acccagttga agacaaacct cacgagtgtc
     4381 acaaagaacc gtgcagataa gaatgctatt cataatcaca ttcgtttgtt tgaacctctt
     4441 gttataaaag ctttaaaaca gtacacgact acaacatgtg tgcagttaca gaagcaggtt
     4501 ttagatttgc tggcgcagct ggttcagtta cgggttaatt actgtcttct ggattcagat
     4561 caggtgttta ttggctttgt attgaaacag tttgaataca ttgaagtggg ccagttcagg
     4621 gaatcagagg caatcattcc aaacatcttt ttcttcttgg tattactatc ttatgaacgc
     4681 tatcattcaa aacagatcat tggaattcct aaaatcattc agctctgtga tggcatcatg
     4741 gccagtggaa ggaaggctgt gacacatgcc ataccggctc tgcagcccat agtccacgac
     4801 ctctttgtat taagaggaac aaataaagct gatgcaggaa aagagcttga aacccaaaaa
     4861 gaggtggtgg tgtcaatgtt actgagactc atccagtacc atcaggtgtt ggagatgttc
     4921 attcttgtcc tgcagcagtg ccacaaggag aatgaagaca agtggaagcg actgtctcga
     4981 cagatagctg acatcatcct cccaatgtta gccaaacagc agatgcacat tgactctcat
     5041 gaagcccttg gagtgttaaa tacattattt gagattttgg ccccttcctc cctccgtccg
     5101 gtagacatgc ttttacggag tatgttcgtc actccaaaca caatggcgtc cgtgagcact
     5161 gttcaactgt ggatatcggg aattctggcc attttgaggg ttctgatttc ccagtcaact
     5221 gaagatattg ttctttctcg tattcaggag ctctccttct ctccgtattt aatctcctgt
     5281 acagtaatta ataggttaag agatggggac agtacttcaa cgctagaaga acacagtgaa
     5341 gggaaacaaa taaagaattt gccagaagaa acattttcaa ggtttctatt acaactggtt
     5401 ggtattcttt tagaagacat tgttacaaaa cagctgaagg tggaaatgag tgagcagcaa
     5461 catactttct attgccagga actaggcaca ctgctaatgt gtctgatcca catcttcaag
     5521 tctggaatgt tccggagaat cacagcagct gccactaggc tgttccgcag tgatggctgt
     5581 ggcggcagtt tctacaccct ggacagcttg aacttgcggg ctcgttccat gatcaccacc
     5641 cacccggccc tggtgctgct ctggtgtcag atactgctgc ttgtcaacca caccgactac
     5701 cgctggtggg cagaagtgca gcagaccccg aaaagacaca gtctgtccag cacaaagtta
     5761 cttagtcccc agatgtctgg agaagaggag gattctgact tggcagccaa acttggaatg
     5821 tgcaatagag aaatagtacg aagaggggct ctcattctct tctgtgatta tgtctgtcag
     5881 aacctccatg actccgagca cttaacgtgg ctcattgtaa atcacattca agatctgatc
     5941 agcctttccc acgagcctcc agtacaggac ttcatcagtg ccgttcatcg gaactctgct
     6001 gccagcggcc tgttcatcca ggcaattcag tctcgttgtg aaaacctttc aactccaacc
     6061 atgctgaaga aaactcttca gtgcttggag gggatccatc tcagccagtc gggagctgtg
     6121 ctcacgctgt atgtggacag gcttctgtgc acccctttcc gtgtgctggc tcgcatggtc
     6181 gacatccttg cttgtcgccg ggtagaaatg cttctggctg caaatttaca gagcagcatg
     6241 gcccagttgc caatggaaga actcaacaga atccaggaat accttcagag cagcgggctc
     6301 gctcagagac accaaaggct ctattccctg ctggacaggt ttcgtctctc caccatgcaa
     6361 gactcactta gtccctctcc tccagtctct tcccacccgc tggacgggga tgggcacgtg
     6421 tcactggaaa cagtgagtcc ggacaaagac tggtacgttc atcttgtcaa atcccagtgt
     6481 tggaccaggt cagattctgc actgctggaa ggtgcagagc tggtgaatcg gattcctgct
     6541 gaagatatga atgccttcat gatgaactcg gagttcaacc taagcctgct agctccatgc
     6601 ttaagcctag ggatgagtga aatttctggt ggccagaaga gtgccctttt tgaagcagcc
     6661 cgtgaggtga ctctggcccg tgtgagcggc accgtgcagc agctccctgc tgtccatcat
     6721 gtcttccagc ccgagctgcc tgcagaaccg gcggcctact ggagcaagtt gaatgatctg
     6781 tttggggatg ctgcactgta tcagtccctg cccactctgg cccgggccct ggcacagtac
     6841 ctggtggtgg tctccaaact gcccagtcat ttgcaccttc ctcctgagaa agagaaggac
     6901 attgtgaaat tcgtggtggc aacccttgag gccctgtcct ggcatttgat ccatgagcag
     6961 atcccgctga gtctggatct ccaggcaggg ctggactgct gctgcctggc cctgcagctg
     7021 cctggcctct ggagcgtggt ctcctccaca gagtttgtga cccacgcctg ctccctcatc
     7081 cactgtgtgc acttcatcct ggaggccgtt gcagtgcagc ctggagagca gcttcttagt
     7141 ccagaaagaa ggacaaatac cccaaaagcc atcagcgagg aggaggagga agtagatcca
     7201 aacacacaga atcctaagta tatcactgca gcctgtgaga tggtggcaga aatggtggag
     7261 tctctgcagt cggtgttggc cttgggtcat aaaaggaata gcggcgtgcc ggcgtttctc
     7321 acgccattgc tcaggaacat catcatcagc ctggcccgcc tgccccttgt caacagctac
     7381 acacgtgtgc ccccactggt gtggaagctt ggatggtcac ccaaaccggg aggggatttt
     7441 ggcacagcat tccctgagat ccccgtggag ttcctccagg aaaaggaagt ctttaaggag
     7501 ttcatctacc gcatcaacac actaggctgg accagtcgta ctcagtttga agaaacttgg
     7561 gccaccctcc ttggtgtcct ggtgacgcag cccctcgtga tggagcagga ggagagccca
     7621 ccagaagaag acacagagag gacccagatc aacgtcctgg ccgtgcaggc catcacctca
     7681 ctggtgctca gtgcaatgac tgtgcctgtg gccggcaacc cagctgtaag ctgcttggag
     7741 cagcagcccc ggaacaagcc tctgaaagct ctcgacacca ggtttgggag gaagctgagc
     7801 attatcagag ggattgtgga gcaagagatt caagcaatgg tttcaaagag agagaatatt
     7861 gccacccatc atttatatca ggcatgggat cctgtccctt ctctgtctcc ggctactaca
     7921 ggtgccctca tcagccacga gaagctgctg ctgcagatca accccgagcg ggagctgggg
     7981 agcatgagct acaaactcgg ccaggtgtcc atacactccg tgtggctagg gaacagcatc
     8041 acacccctga gggaggagga atgggacgag gaagaggagg aggaggccga cgcccctgca
     8101 ccttcgtcac cacccacgtc tccagtcaac tccaggaaac accgggctgg agttgacatc
     8161 cactcctgtt cgcagttttt gcttgagttg tacagccgct ggatcctgcc gtccagctca
     8221 gccaggagga ccccggccat cctgatcagt gaggtggtca gatcccttct agtggtctca
     8281 gacttgttca ccgagcgcaa ccagtttgag ctgatgtatg tgacgctgac agaactgcga
     8341 agggtgcacc cttcagaaga cgagatcctc gctcagtacc tggtgcctgc cacctgcaag
     8401 gcagctgccg tccttgggat ggacaaggcc gtggcggagc ctgtcagccg cctgctggag
     8461 agcacgctca ggagcagcca cctgcccagc agggttggag ccctgcacgg catcctctat
     8521 gtgctggagt gcgacctgct ggacgacact gccaagcagc tcatcccggt catcagcgac
     8581 tatctcctct ccaacctgaa agggatcgcc cactgcgtga acattcacag ccagcagcac
     8641 gtactggtca tgtgtgccac tgcgttttac ctcattgaga actatcctct ggacgtaggg
     8701 ccggaatttt cagcatcaat aatacagatg tgtggggtga tgctgtctgg aagtgaggag
     8761 tccaccccct ccatcattta ccactgtgcc ctcagaggcc tggagcgcct cctgctctct
     8821 gagcagctct cccgcctgga tgcagaatcg ctggtcaagc tgagtgtgga cagagtgaac
     8881 gtgcacagcc cgcaccgggc catggcggct ctgggcctga tgctcacctg catgtacaca
     8941 ggaaaggaga aagtcagtcc gggtagaact tcagacccta atcctgcagc ccccgacagc
     9001 gagtcagtga ttgttgctat ggagcgggta tctgttcttt ttgataggat caggaaaggc
     9061 tttccttgtg aagccagagt ggtggccagg atcctgcccc agtttctaga cgacttcttc
     9121 ccaccccagg acatcatgaa caaagtcatc ggagagtttc tgtccaacca gcagccatac
     9181 ccccagttca tggccaccgt ggtgtataag gtgtttcaga ctctgcacag caccgggcag
     9241 tcgtccatgg tccgggactg ggtcatgctg tccctctcca acttcacgca gagggccccg
     9301 gtcgccatgg ccacgtggag cctctcctgc ttctttgtca gcgcgtccac cagcccgtgg
     9361 gtcgcggcga tcctcccaca tgtcatcagc aggatgggca agctggagca ggtggacgtg
     9421 aaccttttct gcctggtcgc cacagacttc tacagacacc agatagagga ggagctcgac
     9481 cgcagggcct tccagtctgt gcttgaggtg gttgcagccc caggaagccc atatcaccgg
     9541 ctgctgactt gtttacgaaa tgtccacaag gtcaccacct gc
//
