LOCUS       sequence-209881-        9690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9008..9017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacaacagca gcaacagcaa
      181 caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca gcaacaacag
      241 cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca acagcagcaa
      301 cagcaacaac agcagcaaca gcaacaacag cagcaacagc aacaacagca gcaacagcaa
      361 caacagcagc aacagcaacc gccaccgccg ccgccgccgc cgccgcctcc tcagcttcct
      421 cagccgccgc cgcaggcaca gccgctgctg cctcagccgc agccgccccc gccgccgccc
      481 ccgccgccac ccggcccggc tgtggctgag gagccgctgc accgaccaaa gaaagaactt
      541 tcagctacca agaaagaccg tgtgaatcat tgtctgacaa tatgtgaaaa catagtggca
      601 cagtctgtca gaaattctcc agaatttcag aaacttctgg gcatcgctat ggaacttttt
      661 ctgctgtgca gtgatgacgc agagtcagat gtcaggatgg tggctgacga atgcctcaac
      721 aaagttatca aagctttgat ggattctaat cttccaaggt tacagctcga gctctataag
      781 gaaattaaaa agaatggtgc ccctcggagt ttgcgtgctg ccctgtggag gtttgctgag
      841 ctggctcacc tggttcggcc tcagaaatgc aggccttacc tggtgaacct tctgccgtgc
      901 ctgactcgaa caagcaagag acccgaagaa tcagtccagg agaccttggc tgcagctgtt
      961 cccaaaatta tggcttcttt tggcaatttt gcaaatgaca atgaaattaa ggttttgtta
     1021 aaggccttca tagcgaacct gaagtcaagc tcccccacca ttcggcggac agcggctgga
     1081 tcagcagtga gcatctgcca gcactcaaga aggacacaat atttctatag ttggctacta
     1141 aatgtgctct taggcttact cgttcctgtc gaggatgaac actccactct gctgattctt
     1201 ggcgtgctgc tcaccctgag gtatttggtg cccttgctgc agcagcaggt caaggacaca
     1261 agcctgaaag gcagcttcgg agtgacaagg aaagaaatgg aagtctctcc ttctgcagag
     1321 cagcttgtcc aggtttatga actgacgtta catcatacac agcaccaaga ccacaatgtt
     1381 gtgaccggag ccctggagct gttgcagcag ctcttcagaa cgcctccacc cgagcttctg
     1441 caaaccctga ccgcagtcgg gggcattggg cagctcaccg ctgctaagga ggagtctggt
     1501 ggccgaagcc gtagtgggag tattgtggaa cttatagctg gagggggttc ctcatgcagc
     1561 cctgtccttt caagaaaaca aaaaggcaaa gtgctcttag gagaagaaga agccttggag
     1621 gatgactctg aatcgagatc ggatgtcagc agctctgcct taacagcctc agtgaaggat
     1681 gagatcagtg gagagctggc tgcttcttca ggggtttcca ctccagggtc agcaggtcat
     1741 gacatcatca cagaacagcc acggtcacag cacacactgc aggcggactc agtggatctg
     1801 gccagctgtg acttgacaag ctctgccact gatggggatg aggaggatat cttgagccac
     1861 agctccagcc aggtcagcgc cgtcccatct gaccctgcca tggacctgaa tgatgggacc
     1921 caggcctcgt cgcccatcag cgacagctcc cagaccacca ccgaagggcc tgattcagct
     1981 gttacccctt cagacagttc tgaaattgtg ttagacggta ccgacaacca gtatttgggc
     2041 ctgcagattg gacagcccca ggatgaagat gaggaagcca caggtattct tcctgatgaa
     2101 gcctcggagg ccttcaggaa ctcttccatg gcccttcaac aggcacattt attgaaaaac
     2161 atgagtcact gcaggcagcc ttctgacagc agtgttgata aatttgtgtt gagagatgaa
     2221 gctactgaac cgggtgatca agaaaacaag ccttgccgca tcaaaggtga cattggacag
     2281 tccactgatg atgactctgc acctcttgtc cattgtgtcc gccttttatc tgcttcgttt
     2341 ttgctaacag ggggaaaaaa tgtgctggtt ccggacaggg atgtgagggt cagcgtgaag
     2401 gccctggccc tcagctgtgt gggagcagct gtggccctcc acccggaatc tttcttcagc
     2461 aaactctata aagttcctct tgacaccacg gaataccctg aggaacagta tgtctcagac
     2521 atcttgaact acatcgatca tggagaccca caggttcgag gagccactgc cattctctgt
     2581 gggaccctca tctgctccat cctcagcagg tcccgcttcc acgtgggaga ttggatgggc
     2641 accattagaa ccctcacagg aaatacattt tctttggcgg attgcattcc tttgctgcgg
     2701 aaaacactga aggatgagtc ttctgttact tgcaagttag cttgtacagc tgtgaggaac
     2761 tgtgtcatga gtctctgcag cagcagctac agtgagttag gactgcagct gatcatcgat
     2821 gtgctgactc tgaggaacag ttcctattgg ctggtgagga cagagcttct ggaaaccctt
     2881 gcagagattg acttcaggct ggtgagcttt ttggaggcaa aagcagaaaa cttacacaga
     2941 ggggctcatc attatacagg gcttttaaaa ctgcaagaac gagtgctcaa taatgttgtc
     3001 atccatttgc ttggagatga agaccccagg gtgcgacatg ttgccgcagc atcactaatt
     3061 aggcttgtcc caaagctgtt ttataaatgt gaccaaggac aagctgatcc agtagtggcc
     3121 gtggcaagag atcaaagcag tgtttacctg aaacttctca tgcatgagac gcagcctcca
     3181 tctcatttct ccgtcagcac aataaccaga atatatagag gctataacct actaccaagc
     3241 ataacagacg tcactatgga aaataacctt tcaagagtta ttgcagcagt ttctcatgaa
     3301 ctaatcacat caaccaccag agcactcaca tttggatgct gtgaagcttt gtgtcttctt
     3361 tccactgcct tcccagtttg catttggagt ttaggttggc actgtggagt gcctccactg
     3421 agtgcctcag atgagtctag gaagagctgt accgttggga tggccacaat gattctgacc
     3481 ctgctctcgt cagcttggtt cccattggat ctctcagccc atcaagatgc tttgattttg
     3541 gccggaaact tgcttgcagc cagtgctccc aaatctctga gaagttcatg ggcctctgaa
     3601 gaagaagcca acccagcagc caccaagcaa gaggaggtct ggccagccct gggggaccgg
     3661 gccctggtgc ccatggtgga gcagctcttc tctcacctgc tgaaggtgat taacatttgt
     3721 gcccacgtcc tggatgacgt ggctcctgga cccgcaataa aggcagcctt gccttctcta
     3781 acaaaccccc cttctctaag tcccatccga cgaaagggga aggagaaaga accaggagaa
     3841 caagcatctg taccgttgag tcccaagaaa ggcagtgagg ccagtgcagc ttctagacaa
     3901 tctgatacct caggtcctgt tacaacaagt aaatcctcat cactggggag tttctatcat
     3961 cttccttcat acctcaaact gcatgatgtc ctgaaagcta cacacgctaa ctacaaggtc
     4021 acgctggatc ttcagaacag cacggaaaag tttggagggt ttcttcgctc agccttggat
     4081 gttctttctc agatactaga gctggccaca ctgcaggaca ttgggaagtg tgttgaagag
     4141 atcctaggat acctgaaatc ctgctttagt cgagaaccaa tgatggcaac tgtttgtgtt
     4201 caacaattgt tgaagactct ctttggcaca aacttggcct cccagtttga tggcttatct
     4261 tccaacccca gcaagtcaca aggccgagca cagcgccttg gctcctccag tgtgaggcca
     4321 ggcttgtacc actactgctt catggccccg tacacccact tcacccaggc cctcgctgac
     4381 gccagcctga ggaacatggt gcaggcggag caggagaacg acacctcggg atggtttgat
     4441 gtcctccaga aagtgtctac ccagttgaag acaaacctca cgagtgtcac aaagaaccgt
     4501 gcagataaga atgctattca taatcacatt cgtttgtttg aacctcttgt tataaaagct
     4561 ttaaaacagt acacgactac aacatgtgtg cagttacaga agcaggtttt agatttgctg
     4621 gcgcagctgg ttcagttacg ggttaattac tgtcttctgg attcagatca ggtgtttatt
     4681 ggctttgtat tgaaacagtt tgaatacatt gaagtgggcc agttcaggga atcagaggca
     4741 atcattccaa acatcttttt cttcttggta ttactatctt atgaacgcta tcattcaaaa
     4801 cagatcattg gaattcctaa aatcattcag ctctgtgatg gcatcatggc cagtggaagg
     4861 aaggctgtga cacatgccat accggctctg cagcccatag tccacgacct ctttgtatta
     4921 agaggaacaa ataaagctga tgcaggaaaa gagcttgaaa cccaaaaaga ggtggtggtg
     4981 tcaatgttac tgagactcat ccagtaccat caggtgttgg agatgttcat tcttgtcctg
     5041 cagcagtgcc acaaggagaa tgaagacaag tggaagcgac tgtctcgaca gatagctgac
     5101 atcatcctcc caatgttagc caaacagcag atgcacattg actctcatga agcccttgga
     5161 gtgttaaata cattatttga gattttggcc ccttcctccc tccgtccggt agacatgctt
     5221 ttacggagta tgttcgtcac tccaaacaca atggcgtccg tgagcactgt tcaactgtgg
     5281 atatcgggaa ttctggccat tttgagggtt ctgatttccc agtcaactga agatattgtt
     5341 ctttctcgta ttcaggagct ctccttctct ccgtatttaa tctcctgtac agtaattaat
     5401 aggttaagag atggggacag tacttcaacg ctagaagaac acagtgaagg gaaacaaata
     5461 aagaatttgc cagaagaaac attttcaagg tttctattac aactggttgg tattctttta
     5521 gaagacattg ttacaaaaca gctgaaggtg gaaatgagtg agcagcaaca tactttctat
     5581 tgccaggaac taggcacact gctaatgtgt ctgatccaca tcttcaagtc tggaatgttc
     5641 cggagaatca cagcagctgc cactaggctg ttccgcagtg atggctgtgg cggcagtttc
     5701 tacaccctgg acagcttgaa cttgcgggct cgttccatga tcaccaccca cccggccctg
     5761 gtgctgctct ggtgtcagat actgctgctt gtcaaccaca ccgactaccg ctggtgggca
     5821 gaagtgcagc agaccccgaa aagacacagt ctgtccagca caaagttact tagtccccag
     5881 atgtctggag aagaggagga ttctgacttg gcagccaaac ttggaatgtg caatagagaa
     5941 atagtacgaa gaggggctct cattctcttc tgtgattatg tctgtcagaa cctccatgac
     6001 tccgagcact taacgtggct cattgtaaat cacattcaag atctgatcag cctttcccac
     6061 gagcctccag tacaggactt catcagtgcc gttcatcgga actctgctgc cagcggcctg
     6121 ttcatccagg caattcagtc tcgttgtgaa aacctttcaa ctccaaccat gctgaagaaa
     6181 actcttcagt gcttggaggg gatccatctc agccagtcgg gagctgtgct cacgctgtat
     6241 gtggacaggc ttctgtgcac ccctttccgt gtgctggctc gcatggtcga catccttgct
     6301 tgtcgccggg tagaaatgct tctggctgca aatttacaga gcagcatggc ccagttgcca
     6361 atggaagaac tcaacagaat ccaggaatac cttcagagca gcgggctcgc tcagagacac
     6421 caaaggctct attccctgct ggacaggttt cgtctctcca ccatgcaaga ctcacttagt
     6481 ccctctcctc cagtctcttc ccacccgctg gacggggatg ggcacgtgtc actggaaaca
     6541 gtgagtccgg acaaagactg gtacgttcat cttgtcaaat cccagtgttg gaccaggtca
     6601 gattctgcac tgctggaagg tgcagagctg gtgaatcgga ttcctgctga agatatgaat
     6661 gccttcatga tgaactcgga gttcaaccta agcctgctag ctccatgctt aagcctaggg
     6721 atgagtgaaa tttctggtgg ccagaagagt gccctttttg aagcagcccg tgaggtgact
     6781 ctggcccgtg tgagcggcac cgtgcagcag ctccctgctg tccatcatgt cttccagccc
     6841 gagctgcctg cagaaccggc ggcctactgg agcaagttga atgatctgtt tggggatgct
     6901 gcactgtatc agtccctgcc cactctggcc cgggccctgg cacagtacct ggtggtggtc
     6961 tccaaactgc ccagtcattt gcaccttcct cctgagaaag agaaggacat tgtgaaattc
     7021 gtggtggcaa cccttgaggc cctgtcctgg catttgatcc atgagcagat cccgctgagt
     7081 ctggatctcc aggcagggct ggactgctgc tgcctggccc tgcagctgcc tggcctctgg
     7141 agcgtggtct cctccacaga gtttgtgacc cacgcctgct ccctcatcca ctgtgtgcac
     7201 ttcatcctgg aggccgttgc agtgcagcct ggagagcagc ttcttagtcc agaaagaagg
     7261 acaaataccc caaaagccat cagcgaggag gaggaggaag tagatccaaa cacacagaat
     7321 cctaagtata tcactgcagc ctgtgagatg gtggcagaaa tggtggagtc tctgcagtcg
     7381 gtgttggcct tgggtcataa aaggaatagc ggcgtgccgg cgtttctcac gccattgctc
     7441 aggaacatca tcatcagcct ggcccgcctg ccccttgtca acagctacac acgtgtgccc
     7501 ccactggtgt ggaagcttgg atggtcaccc aaaccgggag gggattttgg cacagcattc
     7561 cctgagatcc ccgtggagtt cctccaggaa aaggaagtct ttaaggagtt catctaccgc
     7621 atcaacacac taggctggac cagtcgtact cagtttgaag aaacttgggc caccctcctt
     7681 ggtgtcctgg tgacgcagcc cctcgtgatg gagcaggagg agagcccacc agaagaagac
     7741 acagagagga cccagatcaa cgtcctggcc gtgcaggcca tcacctcact ggtgctcagt
     7801 gcaatgactg tgcctgtggc cggcaaccca gctgtaagct gcttggagca gcagccccgg
     7861 aacaagcctc tgaaagctct cgacaccagg tttgggagga agctgagcat tatcagaggg
     7921 attgtggagc aagagattca agcaatggtt tcaaagagag agaatattgc cacccatcat
     7981 ttatatcagg catgggatcc tgtcccttct ctgtctccgg ctactacagg tgccctcatc
     8041 agccacgaga agctgctgct gcagatcaac cccgagcggg agctggggag catgagctac
     8101 aaactcggcc aggtgtccat acactccgtg tggctaggga acagcatcac acccctgagg
     8161 gaggaggaat gggacgagga agaggaggag gaggccgacg cccctgcacc ttcgtcacca
     8221 cccacgtctc cagtcaactc caggaaacac cgggctggag ttgacatcca ctcctgttcg
     8281 cagtttttgc ttgagttgta cagccgctgg atcctgccgt ccagctcagc caggaggacc
     8341 ccggccatcc tgatcagtga ggtggtcaga tcccttctag tggtctcaga cttgttcacc
     8401 gagcgcaacc agtttgagct gatgtatgtg acgctgacag aactgcgaag ggtgcaccct
     8461 tcagaagacg agatcctcgc tcagtacctg gtgcctgcca cctgcaaggc agctgccgtc
     8521 cttgggatgg acaaggccgt ggcggagcct gtcagccgcc tgctggagag cacgctcagg
     8581 agcagccacc tgcccagcag ggttggagcc ctgcacggca tcctctatgt gctggagtgc
     8641 gacctgctgg acgacactgc caagcagctc atcccggtca tcagcgacta tctcctctcc
     8701 aacctgaaag ggatcgccca ctgcgtgaac attcacagcc agcagcacgt actggtcatg
     8761 tgtgccactg cgttttacct cattgagaac tatcctctgg acgtagggcc ggaattttca
     8821 gcatcaataa tacagatgtg tggggtgatg ctgtctggaa gtgaggagtc caccccctcc
     8881 atcatttacc actgtgccct cagaggcctg gagcgcctcc tgctctctga gcagctctcc
     8941 cgcctggatg cagaatcgct ggtcaagctg agtgtggaca gagtgaacgt gcacagcccg
     9001 caccgggcca tggcggctct gggcctgatg ctcacctgca tgtacacagg aaaggagaaa
     9061 gtcagtccgg gtagaacttc agaccctaat cctgcagccc ccgacagcga gtcagtgatt
     9121 gttgctatgg agcgggtatc tgttcttttt gataggatca ggaaaggctt tccttgtgaa
     9181 gccagagtgg tggccaggat cctgccccag tttctagacg acttcttccc accccaggac
     9241 atcatgaaca aagtcatcgg agagtttctg tccaaccagc agccataccc ccagttcatg
     9301 gccaccgtgg tgtataaggt gtttcagact ctgcacagca ccgggcagtc gtccatggtc
     9361 cgggactggg tcatgctgtc cctctccaac ttcacgcaga gggccccggt cgccatggcc
     9421 acgtggagcc tctcctgctt ctttgtcagc gcgtccacca gcccgtgggt cgcggcgatc
     9481 ctcccacatg tcatcagcag gatgggcaag ctggagcagg tggacgtgaa ccttttctgc
     9541 ctggtcgcca cagacttcta cagacaccag atagaggagg agctcgaccg cagggccttc
     9601 cagtctgtgc ttgaggtggt tgcagcccca ggaagcccat atcaccggct gctgacttgt
     9661 ttacgaaatg tccacaaggt caccacctgc
//
