LOCUS       sequence-209882-        9798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9116..9125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      241 caacagcaac aacagcagca acagcaacaa caacagcagc aacagcaaca acagcagcaa
      301 cagcaacaac agcagcaaca gcaacaacag cagcaacagc aacaacagca gcaacagcaa
      361 caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca gcaacaacag
      421 cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca acagcagcaa
      481 cagcaaccgc caccgccgcc gccgccgccg ccgcctcctc agcttcctca gccgccgccg
      541 caggcacagc cgctgctgcc tcagccgcag ccgcccccgc cgccgccccc gccgccaccc
      601 ggcccggctg tggctgagga gccgctgcac cgaccaaaga aagaactttc agctaccaag
      661 aaagaccgtg tgaatcattg tctgacaata tgtgaaaaca tagtggcaca gtctgtcaga
      721 aattctccag aatttcagaa acttctgggc atcgctatgg aactttttct gctgtgcagt
      781 gatgacgcag agtcagatgt caggatggtg gctgacgaat gcctcaacaa agttatcaaa
      841 gctttgatgg attctaatct tccaaggtta cagctcgagc tctataagga aattaaaaag
      901 aatggtgccc ctcggagttt gcgtgctgcc ctgtggaggt ttgctgagct ggctcacctg
      961 gttcggcctc agaaatgcag gccttacctg gtgaaccttc tgccgtgcct gactcgaaca
     1021 agcaagagac ccgaagaatc agtccaggag accttggctg cagctgttcc caaaattatg
     1081 gcttcttttg gcaattttgc aaatgacaat gaaattaagg ttttgttaaa ggccttcata
     1141 gcgaacctga agtcaagctc ccccaccatt cggcggacag cggctggatc agcagtgagc
     1201 atctgccagc actcaagaag gacacaatat ttctatagtt ggctactaaa tgtgctctta
     1261 ggcttactcg ttcctgtcga ggatgaacac tccactctgc tgattcttgg cgtgctgctc
     1321 accctgaggt atttggtgcc cttgctgcag cagcaggtca aggacacaag cctgaaaggc
     1381 agcttcggag tgacaaggaa agaaatggaa gtctctcctt ctgcagagca gcttgtccag
     1441 gtttatgaac tgacgttaca tcatacacag caccaagacc acaatgttgt gaccggagcc
     1501 ctggagctgt tgcagcagct cttcagaacg cctccacccg agcttctgca aaccctgacc
     1561 gcagtcgggg gcattgggca gctcaccgct gctaaggagg agtctggtgg ccgaagccgt
     1621 agtgggagta ttgtggaact tatagctgga gggggttcct catgcagccc tgtcctttca
     1681 agaaaacaaa aaggcaaagt gctcttagga gaagaagaag ccttggagga tgactctgaa
     1741 tcgagatcgg atgtcagcag ctctgcctta acagcctcag tgaaggatga gatcagtgga
     1801 gagctggctg cttcttcagg ggtttccact ccagggtcag caggtcatga catcatcaca
     1861 gaacagccac ggtcacagca cacactgcag gcggactcag tggatctggc cagctgtgac
     1921 ttgacaagct ctgccactga tggggatgag gaggatatct tgagccacag ctccagccag
     1981 gtcagcgccg tcccatctga ccctgccatg gacctgaatg atgggaccca ggcctcgtcg
     2041 cccatcagcg acagctccca gaccaccacc gaagggcctg attcagctgt taccccttca
     2101 gacagttctg aaattgtgtt agacggtacc gacaaccagt atttgggcct gcagattgga
     2161 cagccccagg atgaagatga ggaagccaca ggtattcttc ctgatgaagc ctcggaggcc
     2221 ttcaggaact cttccatggc ccttcaacag gcacatttat tgaaaaacat gagtcactgc
     2281 aggcagcctt ctgacagcag tgttgataaa tttgtgttga gagatgaagc tactgaaccg
     2341 ggtgatcaag aaaacaagcc ttgccgcatc aaaggtgaca ttggacagtc cactgatgat
     2401 gactctgcac ctcttgtcca ttgtgtccgc cttttatctg cttcgttttt gctaacaggg
     2461 ggaaaaaatg tgctggttcc ggacagggat gtgagggtca gcgtgaaggc cctggccctc
     2521 agctgtgtgg gagcagctgt ggccctccac ccggaatctt tcttcagcaa actctataaa
     2581 gttcctcttg acaccacgga ataccctgag gaacagtatg tctcagacat cttgaactac
     2641 atcgatcatg gagacccaca ggttcgagga gccactgcca ttctctgtgg gaccctcatc
     2701 tgctccatcc tcagcaggtc ccgcttccac gtgggagatt ggatgggcac cattagaacc
     2761 ctcacaggaa atacattttc tttggcggat tgcattcctt tgctgcggaa aacactgaag
     2821 gatgagtctt ctgttacttg caagttagct tgtacagctg tgaggaactg tgtcatgagt
     2881 ctctgcagca gcagctacag tgagttagga ctgcagctga tcatcgatgt gctgactctg
     2941 aggaacagtt cctattggct ggtgaggaca gagcttctgg aaacccttgc agagattgac
     3001 ttcaggctgg tgagcttttt ggaggcaaaa gcagaaaact tacacagagg ggctcatcat
     3061 tatacagggc ttttaaaact gcaagaacga gtgctcaata atgttgtcat ccatttgctt
     3121 ggagatgaag accccagggt gcgacatgtt gccgcagcat cactaattag gcttgtccca
     3181 aagctgtttt ataaatgtga ccaaggacaa gctgatccag tagtggccgt ggcaagagat
     3241 caaagcagtg tttacctgaa acttctcatg catgagacgc agcctccatc tcatttctcc
     3301 gtcagcacaa taaccagaat atatagaggc tataacctac taccaagcat aacagacgtc
     3361 actatggaaa ataacctttc aagagttatt gcagcagttt ctcatgaact aatcacatca
     3421 accaccagag cactcacatt tggatgctgt gaagctttgt gtcttctttc cactgccttc
     3481 ccagtttgca tttggagttt aggttggcac tgtggagtgc ctccactgag tgcctcagat
     3541 gagtctagga agagctgtac cgttgggatg gccacaatga ttctgaccct gctctcgtca
     3601 gcttggttcc cattggatct ctcagcccat caagatgctt tgattttggc cggaaacttg
     3661 cttgcagcca gtgctcccaa atctctgaga agttcatggg cctctgaaga agaagccaac
     3721 ccagcagcca ccaagcaaga ggaggtctgg ccagccctgg gggaccgggc cctggtgccc
     3781 atggtggagc agctcttctc tcacctgctg aaggtgatta acatttgtgc ccacgtcctg
     3841 gatgacgtgg ctcctggacc cgcaataaag gcagccttgc cttctctaac aaacccccct
     3901 tctctaagtc ccatccgacg aaaggggaag gagaaagaac caggagaaca agcatctgta
     3961 ccgttgagtc ccaagaaagg cagtgaggcc agtgcagctt ctagacaatc tgatacctca
     4021 ggtcctgtta caacaagtaa atcctcatca ctggggagtt tctatcatct tccttcatac
     4081 ctcaaactgc atgatgtcct gaaagctaca cacgctaact acaaggtcac gctggatctt
     4141 cagaacagca cggaaaagtt tggagggttt cttcgctcag ccttggatgt tctttctcag
     4201 atactagagc tggccacact gcaggacatt gggaagtgtg ttgaagagat cctaggatac
     4261 ctgaaatcct gctttagtcg agaaccaatg atggcaactg tttgtgttca acaattgttg
     4321 aagactctct ttggcacaaa cttggcctcc cagtttgatg gcttatcttc caaccccagc
     4381 aagtcacaag gccgagcaca gcgccttggc tcctccagtg tgaggccagg cttgtaccac
     4441 tactgcttca tggccccgta cacccacttc acccaggccc tcgctgacgc cagcctgagg
     4501 aacatggtgc aggcggagca ggagaacgac acctcgggat ggtttgatgt cctccagaaa
     4561 gtgtctaccc agttgaagac aaacctcacg agtgtcacaa agaaccgtgc agataagaat
     4621 gctattcata atcacattcg tttgtttgaa cctcttgtta taaaagcttt aaaacagtac
     4681 acgactacaa catgtgtgca gttacagaag caggttttag atttgctggc gcagctggtt
     4741 cagttacggg ttaattactg tcttctggat tcagatcagg tgtttattgg ctttgtattg
     4801 aaacagtttg aatacattga agtgggccag ttcagggaat cagaggcaat cattccaaac
     4861 atctttttct tcttggtatt actatcttat gaacgctatc attcaaaaca gatcattgga
     4921 attcctaaaa tcattcagct ctgtgatggc atcatggcca gtggaaggaa ggctgtgaca
     4981 catgccatac cggctctgca gcccatagtc cacgacctct ttgtattaag aggaacaaat
     5041 aaagctgatg caggaaaaga gcttgaaacc caaaaagagg tggtggtgtc aatgttactg
     5101 agactcatcc agtaccatca ggtgttggag atgttcattc ttgtcctgca gcagtgccac
     5161 aaggagaatg aagacaagtg gaagcgactg tctcgacaga tagctgacat catcctccca
     5221 atgttagcca aacagcagat gcacattgac tctcatgaag cccttggagt gttaaataca
     5281 ttatttgaga ttttggcccc ttcctccctc cgtccggtag acatgctttt acggagtatg
     5341 ttcgtcactc caaacacaat ggcgtccgtg agcactgttc aactgtggat atcgggaatt
     5401 ctggccattt tgagggttct gatttcccag tcaactgaag atattgttct ttctcgtatt
     5461 caggagctct ccttctctcc gtatttaatc tcctgtacag taattaatag gttaagagat
     5521 ggggacagta cttcaacgct agaagaacac agtgaaggga aacaaataaa gaatttgcca
     5581 gaagaaacat tttcaaggtt tctattacaa ctggttggta ttcttttaga agacattgtt
     5641 acaaaacagc tgaaggtgga aatgagtgag cagcaacata ctttctattg ccaggaacta
     5701 ggcacactgc taatgtgtct gatccacatc ttcaagtctg gaatgttccg gagaatcaca
     5761 gcagctgcca ctaggctgtt ccgcagtgat ggctgtggcg gcagtttcta caccctggac
     5821 agcttgaact tgcgggctcg ttccatgatc accacccacc cggccctggt gctgctctgg
     5881 tgtcagatac tgctgcttgt caaccacacc gactaccgct ggtgggcaga agtgcagcag
     5941 accccgaaaa gacacagtct gtccagcaca aagttactta gtccccagat gtctggagaa
     6001 gaggaggatt ctgacttggc agccaaactt ggaatgtgca atagagaaat agtacgaaga
     6061 ggggctctca ttctcttctg tgattatgtc tgtcagaacc tccatgactc cgagcactta
     6121 acgtggctca ttgtaaatca cattcaagat ctgatcagcc tttcccacga gcctccagta
     6181 caggacttca tcagtgccgt tcatcggaac tctgctgcca gcggcctgtt catccaggca
     6241 attcagtctc gttgtgaaaa cctttcaact ccaaccatgc tgaagaaaac tcttcagtgc
     6301 ttggagggga tccatctcag ccagtcggga gctgtgctca cgctgtatgt ggacaggctt
     6361 ctgtgcaccc ctttccgtgt gctggctcgc atggtcgaca tccttgcttg tcgccgggta
     6421 gaaatgcttc tggctgcaaa tttacagagc agcatggccc agttgccaat ggaagaactc
     6481 aacagaatcc aggaatacct tcagagcagc gggctcgctc agagacacca aaggctctat
     6541 tccctgctgg acaggtttcg tctctccacc atgcaagact cacttagtcc ctctcctcca
     6601 gtctcttccc acccgctgga cggggatggg cacgtgtcac tggaaacagt gagtccggac
     6661 aaagactggt acgttcatct tgtcaaatcc cagtgttgga ccaggtcaga ttctgcactg
     6721 ctggaaggtg cagagctggt gaatcggatt cctgctgaag atatgaatgc cttcatgatg
     6781 aactcggagt tcaacctaag cctgctagct ccatgcttaa gcctagggat gagtgaaatt
     6841 tctggtggcc agaagagtgc cctttttgaa gcagcccgtg aggtgactct ggcccgtgtg
     6901 agcggcaccg tgcagcagct ccctgctgtc catcatgtct tccagcccga gctgcctgca
     6961 gaaccggcgg cctactggag caagttgaat gatctgtttg gggatgctgc actgtatcag
     7021 tccctgccca ctctggcccg ggccctggca cagtacctgg tggtggtctc caaactgccc
     7081 agtcatttgc accttcctcc tgagaaagag aaggacattg tgaaattcgt ggtggcaacc
     7141 cttgaggccc tgtcctggca tttgatccat gagcagatcc cgctgagtct ggatctccag
     7201 gcagggctgg actgctgctg cctggccctg cagctgcctg gcctctggag cgtggtctcc
     7261 tccacagagt ttgtgaccca cgcctgctcc ctcatccact gtgtgcactt catcctggag
     7321 gccgttgcag tgcagcctgg agagcagctt cttagtccag aaagaaggac aaatacccca
     7381 aaagccatca gcgaggagga ggaggaagta gatccaaaca cacagaatcc taagtatatc
     7441 actgcagcct gtgagatggt ggcagaaatg gtggagtctc tgcagtcggt gttggccttg
     7501 ggtcataaaa ggaatagcgg cgtgccggcg tttctcacgc cattgctcag gaacatcatc
     7561 atcagcctgg cccgcctgcc ccttgtcaac agctacacac gtgtgccccc actggtgtgg
     7621 aagcttggat ggtcacccaa accgggaggg gattttggca cagcattccc tgagatcccc
     7681 gtggagttcc tccaggaaaa ggaagtcttt aaggagttca tctaccgcat caacacacta
     7741 ggctggacca gtcgtactca gtttgaagaa acttgggcca ccctccttgg tgtcctggtg
     7801 acgcagcccc tcgtgatgga gcaggaggag agcccaccag aagaagacac agagaggacc
     7861 cagatcaacg tcctggccgt gcaggccatc acctcactgg tgctcagtgc aatgactgtg
     7921 cctgtggccg gcaacccagc tgtaagctgc ttggagcagc agccccggaa caagcctctg
     7981 aaagctctcg acaccaggtt tgggaggaag ctgagcatta tcagagggat tgtggagcaa
     8041 gagattcaag caatggtttc aaagagagag aatattgcca cccatcattt atatcaggca
     8101 tgggatcctg tcccttctct gtctccggct actacaggtg ccctcatcag ccacgagaag
     8161 ctgctgctgc agatcaaccc cgagcgggag ctggggagca tgagctacaa actcggccag
     8221 gtgtccatac actccgtgtg gctagggaac agcatcacac ccctgaggga ggaggaatgg
     8281 gacgaggaag aggaggagga ggccgacgcc cctgcacctt cgtcaccacc cacgtctcca
     8341 gtcaactcca ggaaacaccg ggctggagtt gacatccact cctgttcgca gtttttgctt
     8401 gagttgtaca gccgctggat cctgccgtcc agctcagcca ggaggacccc ggccatcctg
     8461 atcagtgagg tggtcagatc ccttctagtg gtctcagact tgttcaccga gcgcaaccag
     8521 tttgagctga tgtatgtgac gctgacagaa ctgcgaaggg tgcacccttc agaagacgag
     8581 atcctcgctc agtacctggt gcctgccacc tgcaaggcag ctgccgtcct tgggatggac
     8641 aaggccgtgg cggagcctgt cagccgcctg ctggagagca cgctcaggag cagccacctg
     8701 cccagcaggg ttggagccct gcacggcatc ctctatgtgc tggagtgcga cctgctggac
     8761 gacactgcca agcagctcat cccggtcatc agcgactatc tcctctccaa cctgaaaggg
     8821 atcgcccact gcgtgaacat tcacagccag cagcacgtac tggtcatgtg tgccactgcg
     8881 ttttacctca ttgagaacta tcctctggac gtagggccgg aattttcagc atcaataata
     8941 cagatgtgtg gggtgatgct gtctggaagt gaggagtcca ccccctccat catttaccac
     9001 tgtgccctca gaggcctgga gcgcctcctg ctctctgagc agctctcccg cctggatgca
     9061 gaatcgctgg tcaagctgag tgtggacaga gtgaacgtgc acagcccgca ccgggccatg
     9121 gcggctctgg gcctgatgct cacctgcatg tacacaggaa aggagaaagt cagtccgggt
     9181 agaacttcag accctaatcc tgcagccccc gacagcgagt cagtgattgt tgctatggag
     9241 cgggtatctg ttctttttga taggatcagg aaaggctttc cttgtgaagc cagagtggtg
     9301 gccaggatcc tgccccagtt tctagacgac ttcttcccac cccaggacat catgaacaaa
     9361 gtcatcggag agtttctgtc caaccagcag ccataccccc agttcatggc caccgtggtg
     9421 tataaggtgt ttcagactct gcacagcacc gggcagtcgt ccatggtccg ggactgggtc
     9481 atgctgtccc tctccaactt cacgcagagg gccccggtcg ccatggccac gtggagcctc
     9541 tcctgcttct ttgtcagcgc gtccaccagc ccgtgggtcg cggcgatcct cccacatgtc
     9601 atcagcagga tgggcaagct ggagcaggtg gacgtgaacc ttttctgcct ggtcgccaca
     9661 gacttctaca gacaccagat agaggaggag ctcgaccgca gggccttcca gtctgtgctt
     9721 gaggtggttg cagccccagg aagcccatat caccggctgc tgacttgttt acgaaatgtc
     9781 cacaaggtca ccacctgc
//
