LOCUS       sequence-209883-        9408 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9408
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8726..8735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcaaca acagcagcaa cagcagccgc caccgccgcc gccgccgccg
      121 ccgcctcctc agcttcctca gccgccgccg caggcacagc cgctgctgcc tcagccgcag
      181 ccgcccccgc cgccgccccc gccgccaccc ggcccggctg tggctgagga gccgctgcac
      241 cgaccaaaga aagaactttc agctaccaag aaagaccgtg tgaatcattg tctgacaata
      301 tgtgaaaaca tagtggcaca gtctgtcaga aattctccag aatttcagaa acttctgggc
      361 atcgctatgg aactttttct gctgtgcagt gatgacgcag agtcagatgt caggatggtg
      421 gctgacgaat gcctcaacaa agttatcaaa gctttgatgg attctaatct tccaaggtta
      481 cagctcgagc tctataagga aattaaaaag aatggtgccc ctcggagttt gcgtgctgcc
      541 ctgtggaggt ttgctgagct ggctcacctg gttcggcctc agaaatgcag gccttacctg
      601 gtgaaccttc tgccgtgcct gactcgaaca agcaagagac ccgaagaatc agtccaggag
      661 accttggctg cagctgttcc caaaattatg gcttcttttg gcaattttgc aaatgacaat
      721 gaaattaagg ttttgttaaa ggccttcata gcgaacctga agtcaagctc ccccaccatt
      781 cggcggacag cggctggatc agcagtgagc atctgccagc actcaagaag gacacaatat
      841 ttctatagtt ggctactaaa tgtgctctta ggcttactcg ttcctgtcga ggatgaacac
      901 tccactctgc tgattcttgg cgtgctgctc accctgaggt atttggtgcc cttgctgcag
      961 cagcaggtca aggacacaag cctgaaaggc agcttcggag tgacaaggaa agaaatggaa
     1021 gtctctcctt ctgcagagca gcttgtccag gtttatgaac tgacgttaca tcatacacag
     1081 caccaagacc acaatgttgt gaccggagcc ctggagctgt tgcagcagct cttcagaacg
     1141 cctccacccg agcttctgca aaccctgacc gcagtcgggg gcattgggca gctcaccgct
     1201 gctaaggagg agtctggtgg ccgaagccgt agtgggagta ttgtggaact tatagctgga
     1261 gggggttcct catgcagccc tgtcctttca agaaaacaaa aaggcaaagt gctcttagga
     1321 gaagaagaag ccttggagga tgactctgaa tcgagatcgg atgtcagcag ctctgcctta
     1381 acagcctcag tgaaggatga gatcagtgga gagctggctg cttcttcagg ggtttccact
     1441 ccagggtcag caggtcatga catcatcaca gaacagccac ggtcacagca cacactgcag
     1501 gcggactcag tggatctggc cagctgtgac ttgacaagct ctgccactga tggggatgag
     1561 gaggatatct tgagccacag ctccagccag gtcagcgccg tcccatctga ccctgccatg
     1621 gacctgaatg atgggaccca ggcctcgtcg cccatcagcg acagctccca gaccaccacc
     1681 gaagggcctg attcagctgt taccccttca gacagttctg aaattgtgtt agacggtacc
     1741 gacaaccagt atttgggcct gcagattgga cagccccagg atgaagatga ggaagccaca
     1801 ggtattcttc ctgatgaagc ctcggaggcc ttcaggaact cttccatggc ccttcaacag
     1861 gcacatttat tgaaaaacat gagtcactgc aggcagcctt ctgacagcag tgttgataaa
     1921 tttgtgttga gagatgaagc tactgaaccg ggtgatcaag aaaacaagcc ttgccgcatc
     1981 aaaggtgaca ttggacagtc cactgatgat gactctgcac ctcttgtcca ttgtgtccgc
     2041 cttttatctg cttcgttttt gctaacaggg ggaaaaaatg tgctggttcc ggacagggat
     2101 gtgagggtca gcgtgaaggc cctggccctc agctgtgtgg gagcagctgt ggccctccac
     2161 ccggaatctt tcttcagcaa actctataaa gttcctcttg acaccacgga ataccctgag
     2221 gaacagtatg tctcagacat cttgaactac atcgatcatg gagacccaca ggttcgagga
     2281 gccactgcca ttctctgtgg gaccctcatc tgctccatcc tcagcaggtc ccgcttccac
     2341 gtgggagatt ggatgggcac cattagaacc ctcacaggaa atacattttc tttggcggat
     2401 tgcattcctt tgctgcggaa aacactgaag gatgagtctt ctgttacttg caagttagct
     2461 tgtacagctg tgaggaactg tgtcatgagt ctctgcagca gcagctacag tgagttagga
     2521 ctgcagctga tcatcgatgt gctgactctg aggaacagtt cctattggct ggtgaggaca
     2581 gagcttctgg aaacccttgc agagattgac ttcaggctgg tgagcttttt ggaggcaaaa
     2641 gcagaaaact tacacagagg ggctcatcat tatacagggc ttttaaaact gcaagaacga
     2701 gtgctcaata atgttgtcat ccatttgctt ggagatgaag accccagggt gcgacatgtt
     2761 gccgcagcat cactaattag gcttgtccca aagctgtttt ataaatgtga ccaaggacaa
     2821 gctgatccag tagtggccgt ggcaagagat caaagcagtg tttacctgaa acttctcatg
     2881 catgagacgc agcctccatc tcatttctcc gtcagcacaa taaccagaat atatagaggc
     2941 tataacctac taccaagcat aacagacgtc actatggaaa ataacctttc aagagttatt
     3001 gcagcagttt ctcatgaact aatcacatca accaccagag cactcacatt tggatgctgt
     3061 gaagctttgt gtcttctttc cactgccttc ccagtttgca tttggagttt aggttggcac
     3121 tgtggagtgc ctccactgag tgcctcagat gagtctagga agagctgtac cgttgggatg
     3181 gccacaatga ttctgaccct gctctcgtca gcttggttcc cattggatct ctcagcccat
     3241 caagatgctt tgattttggc cggaaacttg cttgcagcca gtgctcccaa atctctgaga
     3301 agttcatggg cctctgaaga agaagccaac ccagcagcca ccaagcaaga ggaggtctgg
     3361 ccagccctgg gggaccgggc cctggtgccc atggtggagc agctcttctc tcacctgctg
     3421 aaggtgatta acatttgtgc ccacgtcctg gatgacgtgg ctcctggacc cgcaataaag
     3481 gcagccttgc cttctctaac aaacccccct tctctaagtc ccatccgacg aaaggggaag
     3541 gagaaagaac caggagaaca agcatctgta ccgttgagtc ccaagaaagg cagtgaggcc
     3601 agtgcagctt ctagacaatc tgatacctca ggtcctgtta caacaagtaa atcctcatca
     3661 ctggggagtt tctatcatct tccttcatac ctcaaactgc atgatgtcct gaaagctaca
     3721 cacgctaact acaaggtcac gctggatctt cagaacagca cggaaaagtt tggagggttt
     3781 cttcgctcag ccttggatgt tctttctcag atactagagc tggccacact gcaggacatt
     3841 gggaagtgtg ttgaagagat cctaggatac ctgaaatcct gctttagtcg agaaccaatg
     3901 atggcaactg tttgtgttca acaattgttg aagactctct ttggcacaaa cttggcctcc
     3961 cagtttgatg gcttatcttc caaccccagc aagtcacaag gccgagcaca gcgccttggc
     4021 tcctccagtg tgaggccagg cttgtaccac tactgcttca tggccccgta cacccacttc
     4081 acccaggccc tcgctgacgc cagcctgagg aacatggtgc aggcggagca ggagaacgac
     4141 acctcgggat ggtttgatgt cctccagaaa gtgtctaccc agttgaagac aaacctcacg
     4201 agtgtcacaa agaaccgtgc agataagaat gctattcata atcacattcg tttgtttgaa
     4261 cctcttgtta taaaagcttt aaaacagtac acgactacaa catgtgtgca gttacagaag
     4321 caggttttag atttgctggc gcagctggtt cagttacggg ttaattactg tcttctggat
     4381 tcagatcagg tgtttattgg ctttgtattg aaacagtttg aatacattga agtgggccag
     4441 ttcagggaat cagaggcaat cattccaaac atctttttct tcttggtatt actatcttat
     4501 gaacgctatc attcaaaaca gatcattgga attcctaaaa tcattcagct ctgtgatggc
     4561 atcatggcca gtggaaggaa ggctgtgaca catgccatac cggctctgca gcccatagtc
     4621 cacgacctct ttgtattaag aggaacaaat aaagctgatg caggaaaaga gcttgaaacc
     4681 caaaaagagg tggtggtgtc aatgttactg agactcatcc agtaccatca ggtgttggag
     4741 atgttcattc ttgtcctgca gcagtgccac aaggagaatg aagacaagtg gaagcgactg
     4801 tctcgacaga tagctgacat catcctccca atgttagcca aacagcagat gcacattgac
     4861 tctcatgaag cccttggagt gttaaataca ttatttgaga ttttggcccc ttcctccctc
     4921 cgtccggtag acatgctttt acggagtatg ttcgtcactc caaacacaat ggcgtccgtg
     4981 agcactgttc aactgtggat atcgggaatt ctggccattt tgagggttct gatttcccag
     5041 tcaactgaag atattgttct ttctcgtatt caggagctct ccttctctcc gtatttaatc
     5101 tcctgtacag taattaatag gttaagagat ggggacagta cttcaacgct agaagaacac
     5161 agtgaaggga aacaaataaa gaatttgcca gaagaaacat tttcaaggtt tctattacaa
     5221 ctggttggta ttcttttaga agacattgtt acaaaacagc tgaaggtgga aatgagtgag
     5281 cagcaacata ctttctattg ccaggaacta ggcacactgc taatgtgtct gatccacatc
     5341 ttcaagtctg gaatgttccg gagaatcaca gcagctgcca ctaggctgtt ccgcagtgat
     5401 ggctgtggcg gcagtttcta caccctggac agcttgaact tgcgggctcg ttccatgatc
     5461 accacccacc cggccctggt gctgctctgg tgtcagatac tgctgcttgt caaccacacc
     5521 gactaccgct ggtgggcaga agtgcagcag accccgaaaa gacacagtct gtccagcaca
     5581 aagttactta gtccccagat gtctggagaa gaggaggatt ctgacttggc agccaaactt
     5641 ggaatgtgca atagagaaat agtacgaaga ggggctctca ttctcttctg tgattatgtc
     5701 tgtcagaacc tccatgactc cgagcactta acgtggctca ttgtaaatca cattcaagat
     5761 ctgatcagcc tttcccacga gcctccagta caggacttca tcagtgccgt tcatcggaac
     5821 tctgctgcca gcggcctgtt catccaggca attcagtctc gttgtgaaaa cctttcaact
     5881 ccaaccatgc tgaagaaaac tcttcagtgc ttggagggga tccatctcag ccagtcggga
     5941 gctgtgctca cgctgtatgt ggacaggctt ctgtgcaccc ctttccgtgt gctggctcgc
     6001 atggtcgaca tccttgcttg tcgccgggta gaaatgcttc tggctgcaaa tttacagagc
     6061 agcatggccc agttgccaat ggaagaactc aacagaatcc aggaatacct tcagagcagc
     6121 gggctcgctc agagacacca aaggctctat tccctgctgg acaggtttcg tctctccacc
     6181 atgcaagact cacttagtcc ctctcctcca gtctcttccc acccgctgga cggggatggg
     6241 cacgtgtcac tggaaacagt gagtccggac aaagactggt acgttcatct tgtcaaatcc
     6301 cagtgttgga ccaggtcaga ttctgcactg ctggaaggtg cagagctggt gaatcggatt
     6361 cctgctgaag atatgaatgc cttcatgatg aactcggagt tcaacctaag cctgctagct
     6421 ccatgcttaa gcctagggat gagtgaaatt tctggtggcc agaagagtgc cctttttgaa
     6481 gcagcccgtg aggtgactct ggcccgtgtg agcggcaccg tgcagcagct ccctgctgtc
     6541 catcatgtct tccagcccga gctgcctgca gaaccggcgg cctactggag caagttgaat
     6601 gatctgtttg gggatgctgc actgtatcag tccctgccca ctctggcccg ggccctggca
     6661 cagtacctgg tggtggtctc caaactgccc agtcatttgc accttcctcc tgagaaagag
     6721 aaggacattg tgaaattcgt ggtggcaacc cttgaggccc tgtcctggca tttgatccat
     6781 gagcagatcc cgctgagtct ggatctccag gcagggctgg actgctgctg cctggccctg
     6841 cagctgcctg gcctctggag cgtggtctcc tccacagagt ttgtgaccca cgcctgctcc
     6901 ctcatccact gtgtgcactt catcctggag gccgttgcag tgcagcctgg agagcagctt
     6961 cttagtccag aaagaaggac aaatacccca aaagccatca gcgaggagga ggaggaagta
     7021 gatccaaaca cacagaatcc taagtatatc actgcagcct gtgagatggt ggcagaaatg
     7081 gtggagtctc tgcagtcggt gttggccttg ggtcataaaa ggaatagcgg cgtgccggcg
     7141 tttctcacgc cattgctcag gaacatcatc atcagcctgg cccgcctgcc ccttgtcaac
     7201 agctacacac gtgtgccccc actggtgtgg aagcttggat ggtcacccaa accgggaggg
     7261 gattttggca cagcattccc tgagatcccc gtggagttcc tccaggaaaa ggaagtcttt
     7321 aaggagttca tctaccgcat caacacacta ggctggacca gtcgtactca gtttgaagaa
     7381 acttgggcca ccctccttgg tgtcctggtg acgcagcccc tcgtgatgga gcaggaggag
     7441 agcccaccag aagaagacac agagaggacc cagatcaacg tcctggccgt gcaggccatc
     7501 acctcactgg tgctcagtgc aatgactgtg cctgtggccg gcaacccagc tgtaagctgc
     7561 ttggagcagc agccccggaa caagcctctg aaagctctcg acaccaggtt tgggaggaag
     7621 ctgagcatta tcagagggat tgtggagcaa gagattcaag caatggtttc aaagagagag
     7681 aatattgcca cccatcattt atatcaggca tgggatcctg tcccttctct gtctccggct
     7741 actacaggtg ccctcatcag ccacgagaag ctgctgctgc agatcaaccc cgagcgggag
     7801 ctggggagca tgagctacaa actcggccag gtgtccatac actccgtgtg gctagggaac
     7861 agcatcacac ccctgaggga ggaggaatgg gacgaggaag aggaggagga ggccgacgcc
     7921 cctgcacctt cgtcaccacc cacgtctcca gtcaactcca ggaaacaccg ggctggagtt
     7981 gacatccact cctgttcgca gtttttgctt gagttgtaca gccgctggat cctgccgtcc
     8041 agctcagcca ggaggacccc ggccatcctg atcagtgagg tggtcagatc ccttctagtg
     8101 gtctcagact tgttcaccga gcgcaaccag tttgagctga tgtatgtgac gctgacagaa
     8161 ctgcgaaggg tgcacccttc agaagacgag atcctcgctc agtacctggt gcctgccacc
     8221 tgcaaggcag ctgccgtcct tgggatggac aaggccgtgg cggagcctgt cagccgcctg
     8281 ctggagagca cgctcaggag cagccacctg cccagcaggg ttggagccct gcacggcatc
     8341 ctctatgtgc tggagtgcga cctgctggac gacactgcca agcagctcat cccggtcatc
     8401 agcgactatc tcctctccaa cctgaaaggg atcgcccact gcgtgaacat tcacagccag
     8461 cagcacgtac tggtcatgtg tgccactgcg ttttacctca ttgagaacta tcctctggac
     8521 gtagggccgg aattttcagc atcaataata cagatgtgtg gggtgatgct gtctggaagt
     8581 gaggagtcca ccccctccat catttaccac tgtgccctca gaggcctgga gcgcctcctg
     8641 ctctctgagc agctctcccg cctggatgca gaatcgctgg tcaagctgag tgtggacaga
     8701 gtgaacgtgc acagcccgca ccgggccatg gcggctctgg gcctgatgct cacctgcatg
     8761 tacacaggaa aggagaaagt cagtccgggt agaacttcag accctaatcc tgcagccccc
     8821 gacagcgagt cagtgattgt tgctatggag cgggtatctg ttctttttga taggatcagg
     8881 aaaggctttc cttgtgaagc cagagtggtg gccaggatcc tgccccagtt tctagacgac
     8941 ttcttcccac cccaggacat catgaacaaa gtcatcggag agtttctgtc caaccagcag
     9001 ccataccccc agttcatggc caccgtggtg tataaggtgt ttcagactct gcacagcacc
     9061 gggcagtcgt ccatggtccg ggactgggtc atgctgtccc tctccaactt cacgcagagg
     9121 gccccggtcg ccatggccac gtggagcctc tcctgcttct ttgtcagcgc gtccaccagc
     9181 ccgtgggtcg cggcgatcct cccacatgtc atcagcagga tgggcaagct ggagcaggtg
     9241 gacgtgaacc ttttctgcct ggtcgccaca gacttctaca gacaccagat agaggaggag
     9301 ctcgaccgca gggccttcca gtctgtgctt gaggtggttg cagccccagg aagcccatat
     9361 caccggctgc tgacttgttt acgaaatgtc cacaaggtca ccacctgc
//
