LOCUS       sequence-209885-        9516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8834..8843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcaaca acagcagcaa
      181 cagcaacaac agcagcaaca gcaaccgcca ccgccgccgc cgccgccgcc gcctcctcag
      241 cttcctcagc cgccgccgca ggcacagccg ctgctgcctc agccgcagcc gcccccgccg
      301 ccgcccccgc cgccacccgg cccggctgtg gctgaggagc cgctgcaccg accaaagaaa
      361 gaactttcag ctaccaagaa agaccgtgtg aatcattgtc tgacaatatg tgaaaacata
      421 gtggcacagt ctgtcagaaa ttctccagaa tttcagaaac ttctgggcat cgctatggaa
      481 ctttttctgc tgtgcagtga tgacgcagag tcagatgtca ggatggtggc tgacgaatgc
      541 ctcaacaaag ttatcaaagc tttgatggat tctaatcttc caaggttaca gctcgagctc
      601 tataaggaaa ttaaaaagaa tggtgcccct cggagtttgc gtgctgccct gtggaggttt
      661 gctgagctgg ctcacctggt tcggcctcag aaatgcaggc cttacctggt gaaccttctg
      721 ccgtgcctga ctcgaacaag caagagaccc gaagaatcag tccaggagac cttggctgca
      781 gctgttccca aaattatggc ttcttttggc aattttgcaa atgacaatga aattaaggtt
      841 ttgttaaagg ccttcatagc gaacctgaag tcaagctccc ccaccattcg gcggacagcg
      901 gctggatcag cagtgagcat ctgccagcac tcaagaagga cacaatattt ctatagttgg
      961 ctactaaatg tgctcttagg cttactcgtt cctgtcgagg atgaacactc cactctgctg
     1021 attcttggcg tgctgctcac cctgaggtat ttggtgccct tgctgcagca gcaggtcaag
     1081 gacacaagcc tgaaaggcag cttcggagtg acaaggaaag aaatggaagt ctctccttct
     1141 gcagagcagc ttgtccaggt ttatgaactg acgttacatc atacacagca ccaagaccac
     1201 aatgttgtga ccggagccct ggagctgttg cagcagctct tcagaacgcc tccacccgag
     1261 cttctgcaaa ccctgaccgc agtcgggggc attgggcagc tcaccgctgc taaggaggag
     1321 tctggtggcc gaagccgtag tgggagtatt gtggaactta tagctggagg gggttcctca
     1381 tgcagccctg tcctttcaag aaaacaaaaa ggcaaagtgc tcttaggaga agaagaagcc
     1441 ttggaggatg actctgaatc gagatcggat gtcagcagct ctgccttaac agcctcagtg
     1501 aaggatgaga tcagtggaga gctggctgct tcttcagggg tttccactcc agggtcagca
     1561 ggtcatgaca tcatcacaga acagccacgg tcacagcaca cactgcaggc ggactcagtg
     1621 gatctggcca gctgtgactt gacaagctct gccactgatg gggatgagga ggatatcttg
     1681 agccacagct ccagccaggt cagcgccgtc ccatctgacc ctgccatgga cctgaatgat
     1741 gggacccagg cctcgtcgcc catcagcgac agctcccaga ccaccaccga agggcctgat
     1801 tcagctgtta ccccttcaga cagttctgaa attgtgttag acggtaccga caaccagtat
     1861 ttgggcctgc agattggaca gccccaggat gaagatgagg aagccacagg tattcttcct
     1921 gatgaagcct cggaggcctt caggaactct tccatggccc ttcaacaggc acatttattg
     1981 aaaaacatga gtcactgcag gcagccttct gacagcagtg ttgataaatt tgtgttgaga
     2041 gatgaagcta ctgaaccggg tgatcaagaa aacaagcctt gccgcatcaa aggtgacatt
     2101 ggacagtcca ctgatgatga ctctgcacct cttgtccatt gtgtccgcct tttatctgct
     2161 tcgtttttgc taacaggggg aaaaaatgtg ctggttccgg acagggatgt gagggtcagc
     2221 gtgaaggccc tggccctcag ctgtgtggga gcagctgtgg ccctccaccc ggaatctttc
     2281 ttcagcaaac tctataaagt tcctcttgac accacggaat accctgagga acagtatgtc
     2341 tcagacatct tgaactacat cgatcatgga gacccacagg ttcgaggagc cactgccatt
     2401 ctctgtggga ccctcatctg ctccatcctc agcaggtccc gcttccacgt gggagattgg
     2461 atgggcacca ttagaaccct cacaggaaat acattttctt tggcggattg cattcctttg
     2521 ctgcggaaaa cactgaagga tgagtcttct gttacttgca agttagcttg tacagctgtg
     2581 aggaactgtg tcatgagtct ctgcagcagc agctacagtg agttaggact gcagctgatc
     2641 atcgatgtgc tgactctgag gaacagttcc tattggctgg tgaggacaga gcttctggaa
     2701 acccttgcag agattgactt caggctggtg agctttttgg aggcaaaagc agaaaactta
     2761 cacagagggg ctcatcatta tacagggctt ttaaaactgc aagaacgagt gctcaataat
     2821 gttgtcatcc atttgcttgg agatgaagac cccagggtgc gacatgttgc cgcagcatca
     2881 ctaattaggc ttgtcccaaa gctgttttat aaatgtgacc aaggacaagc tgatccagta
     2941 gtggccgtgg caagagatca aagcagtgtt tacctgaaac ttctcatgca tgagacgcag
     3001 cctccatctc atttctccgt cagcacaata accagaatat atagaggcta taacctacta
     3061 ccaagcataa cagacgtcac tatggaaaat aacctttcaa gagttattgc agcagtttct
     3121 catgaactaa tcacatcaac caccagagca ctcacatttg gatgctgtga agctttgtgt
     3181 cttctttcca ctgccttccc agtttgcatt tggagtttag gttggcactg tggagtgcct
     3241 ccactgagtg cctcagatga gtctaggaag agctgtaccg ttgggatggc cacaatgatt
     3301 ctgaccctgc tctcgtcagc ttggttccca ttggatctct cagcccatca agatgctttg
     3361 attttggccg gaaacttgct tgcagccagt gctcccaaat ctctgagaag ttcatgggcc
     3421 tctgaagaag aagccaaccc agcagccacc aagcaagagg aggtctggcc agccctgggg
     3481 gaccgggccc tggtgcccat ggtggagcag ctcttctctc acctgctgaa ggtgattaac
     3541 atttgtgccc acgtcctgga tgacgtggct cctggacccg caataaaggc agccttgcct
     3601 tctctaacaa accccccttc tctaagtccc atccgacgaa aggggaagga gaaagaacca
     3661 ggagaacaag catctgtacc gttgagtccc aagaaaggca gtgaggccag tgcagcttct
     3721 agacaatctg atacctcagg tcctgttaca acaagtaaat cctcatcact ggggagtttc
     3781 tatcatcttc cttcatacct caaactgcat gatgtcctga aagctacaca cgctaactac
     3841 aaggtcacgc tggatcttca gaacagcacg gaaaagtttg gagggtttct tcgctcagcc
     3901 ttggatgttc tttctcagat actagagctg gccacactgc aggacattgg gaagtgtgtt
     3961 gaagagatcc taggatacct gaaatcctgc tttagtcgag aaccaatgat ggcaactgtt
     4021 tgtgttcaac aattgttgaa gactctcttt ggcacaaact tggcctccca gtttgatggc
     4081 ttatcttcca accccagcaa gtcacaaggc cgagcacagc gccttggctc ctccagtgtg
     4141 aggccaggct tgtaccacta ctgcttcatg gccccgtaca cccacttcac ccaggccctc
     4201 gctgacgcca gcctgaggaa catggtgcag gcggagcagg agaacgacac ctcgggatgg
     4261 tttgatgtcc tccagaaagt gtctacccag ttgaagacaa acctcacgag tgtcacaaag
     4321 aaccgtgcag ataagaatgc tattcataat cacattcgtt tgtttgaacc tcttgttata
     4381 aaagctttaa aacagtacac gactacaaca tgtgtgcagt tacagaagca ggttttagat
     4441 ttgctggcgc agctggttca gttacgggtt aattactgtc ttctggattc agatcaggtg
     4501 tttattggct ttgtattgaa acagtttgaa tacattgaag tgggccagtt cagggaatca
     4561 gaggcaatca ttccaaacat ctttttcttc ttggtattac tatcttatga acgctatcat
     4621 tcaaaacaga tcattggaat tcctaaaatc attcagctct gtgatggcat catggccagt
     4681 ggaaggaagg ctgtgacaca tgccataccg gctctgcagc ccatagtcca cgacctcttt
     4741 gtattaagag gaacaaataa agctgatgca ggaaaagagc ttgaaaccca aaaagaggtg
     4801 gtggtgtcaa tgttactgag actcatccag taccatcagg tgttggagat gttcattctt
     4861 gtcctgcagc agtgccacaa ggagaatgaa gacaagtgga agcgactgtc tcgacagata
     4921 gctgacatca tcctcccaat gttagccaaa cagcagatgc acattgactc tcatgaagcc
     4981 cttggagtgt taaatacatt atttgagatt ttggcccctt cctccctccg tccggtagac
     5041 atgcttttac ggagtatgtt cgtcactcca aacacaatgg cgtccgtgag cactgttcaa
     5101 ctgtggatat cgggaattct ggccattttg agggttctga tttcccagtc aactgaagat
     5161 attgttcttt ctcgtattca ggagctctcc ttctctccgt atttaatctc ctgtacagta
     5221 attaataggt taagagatgg ggacagtact tcaacgctag aagaacacag tgaagggaaa
     5281 caaataaaga atttgccaga agaaacattt tcaaggtttc tattacaact ggttggtatt
     5341 cttttagaag acattgttac aaaacagctg aaggtggaaa tgagtgagca gcaacatact
     5401 ttctattgcc aggaactagg cacactgcta atgtgtctga tccacatctt caagtctgga
     5461 atgttccgga gaatcacagc agctgccact aggctgttcc gcagtgatgg ctgtggcggc
     5521 agtttctaca ccctggacag cttgaacttg cgggctcgtt ccatgatcac cacccacccg
     5581 gccctggtgc tgctctggtg tcagatactg ctgcttgtca accacaccga ctaccgctgg
     5641 tgggcagaag tgcagcagac cccgaaaaga cacagtctgt ccagcacaaa gttacttagt
     5701 ccccagatgt ctggagaaga ggaggattct gacttggcag ccaaacttgg aatgtgcaat
     5761 agagaaatag tacgaagagg ggctctcatt ctcttctgtg attatgtctg tcagaacctc
     5821 catgactccg agcacttaac gtggctcatt gtaaatcaca ttcaagatct gatcagcctt
     5881 tcccacgagc ctccagtaca ggacttcatc agtgccgttc atcggaactc tgctgccagc
     5941 ggcctgttca tccaggcaat tcagtctcgt tgtgaaaacc tttcaactcc aaccatgctg
     6001 aagaaaactc ttcagtgctt ggaggggatc catctcagcc agtcgggagc tgtgctcacg
     6061 ctgtatgtgg acaggcttct gtgcacccct ttccgtgtgc tggctcgcat ggtcgacatc
     6121 cttgcttgtc gccgggtaga aatgcttctg gctgcaaatt tacagagcag catggcccag
     6181 ttgccaatgg aagaactcaa cagaatccag gaataccttc agagcagcgg gctcgctcag
     6241 agacaccaaa ggctctattc cctgctggac aggtttcgtc tctccaccat gcaagactca
     6301 cttagtccct ctcctccagt ctcttcccac ccgctggacg gggatgggca cgtgtcactg
     6361 gaaacagtga gtccggacaa agactggtac gttcatcttg tcaaatccca gtgttggacc
     6421 aggtcagatt ctgcactgct ggaaggtgca gagctggtga atcggattcc tgctgaagat
     6481 atgaatgcct tcatgatgaa ctcggagttc aacctaagcc tgctagctcc atgcttaagc
     6541 ctagggatga gtgaaatttc tggtggccag aagagtgccc tttttgaagc agcccgtgag
     6601 gtgactctgg cccgtgtgag cggcaccgtg cagcagctcc ctgctgtcca tcatgtcttc
     6661 cagcccgagc tgcctgcaga accggcggcc tactggagca agttgaatga tctgtttggg
     6721 gatgctgcac tgtatcagtc cctgcccact ctggcccggg ccctggcaca gtacctggtg
     6781 gtggtctcca aactgcccag tcatttgcac cttcctcctg agaaagagaa ggacattgtg
     6841 aaattcgtgg tggcaaccct tgaggccctg tcctggcatt tgatccatga gcagatcccg
     6901 ctgagtctgg atctccaggc agggctggac tgctgctgcc tggccctgca gctgcctggc
     6961 ctctggagcg tggtctcctc cacagagttt gtgacccacg cctgctccct catccactgt
     7021 gtgcacttca tcctggaggc cgttgcagtg cagcctggag agcagcttct tagtccagaa
     7081 agaaggacaa ataccccaaa agccatcagc gaggaggagg aggaagtaga tccaaacaca
     7141 cagaatccta agtatatcac tgcagcctgt gagatggtgg cagaaatggt ggagtctctg
     7201 cagtcggtgt tggccttggg tcataaaagg aatagcggcg tgccggcgtt tctcacgcca
     7261 ttgctcagga acatcatcat cagcctggcc cgcctgcccc ttgtcaacag ctacacacgt
     7321 gtgcccccac tggtgtggaa gcttggatgg tcacccaaac cgggagggga ttttggcaca
     7381 gcattccctg agatccccgt ggagttcctc caggaaaagg aagtctttaa ggagttcatc
     7441 taccgcatca acacactagg ctggaccagt cgtactcagt ttgaagaaac ttgggccacc
     7501 ctccttggtg tcctggtgac gcagcccctc gtgatggagc aggaggagag cccaccagaa
     7561 gaagacacag agaggaccca gatcaacgtc ctggccgtgc aggccatcac ctcactggtg
     7621 ctcagtgcaa tgactgtgcc tgtggccggc aacccagctg taagctgctt ggagcagcag
     7681 ccccggaaca agcctctgaa agctctcgac accaggtttg ggaggaagct gagcattatc
     7741 agagggattg tggagcaaga gattcaagca atggtttcaa agagagagaa tattgccacc
     7801 catcatttat atcaggcatg ggatcctgtc ccttctctgt ctccggctac tacaggtgcc
     7861 ctcatcagcc acgagaagct gctgctgcag atcaaccccg agcgggagct ggggagcatg
     7921 agctacaaac tcggccaggt gtccatacac tccgtgtggc tagggaacag catcacaccc
     7981 ctgagggagg aggaatggga cgaggaagag gaggaggagg ccgacgcccc tgcaccttcg
     8041 tcaccaccca cgtctccagt caactccagg aaacaccggg ctggagttga catccactcc
     8101 tgttcgcagt ttttgcttga gttgtacagc cgctggatcc tgccgtccag ctcagccagg
     8161 aggaccccgg ccatcctgat cagtgaggtg gtcagatccc ttctagtggt ctcagacttg
     8221 ttcaccgagc gcaaccagtt tgagctgatg tatgtgacgc tgacagaact gcgaagggtg
     8281 cacccttcag aagacgagat cctcgctcag tacctggtgc ctgccacctg caaggcagct
     8341 gccgtccttg ggatggacaa ggccgtggcg gagcctgtca gccgcctgct ggagagcacg
     8401 ctcaggagca gccacctgcc cagcagggtt ggagccctgc acggcatcct ctatgtgctg
     8461 gagtgcgacc tgctggacga cactgccaag cagctcatcc cggtcatcag cgactatctc
     8521 ctctccaacc tgaaagggat cgcccactgc gtgaacattc acagccagca gcacgtactg
     8581 gtcatgtgtg ccactgcgtt ttacctcatt gagaactatc ctctggacgt agggccggaa
     8641 ttttcagcat caataataca gatgtgtggg gtgatgctgt ctggaagtga ggagtccacc
     8701 ccctccatca tttaccactg tgccctcaga ggcctggagc gcctcctgct ctctgagcag
     8761 ctctcccgcc tggatgcaga atcgctggtc aagctgagtg tggacagagt gaacgtgcac
     8821 agcccgcacc gggccatggc ggctctgggc ctgatgctca cctgcatgta cacaggaaag
     8881 gagaaagtca gtccgggtag aacttcagac cctaatcctg cagcccccga cagcgagtca
     8941 gtgattgttg ctatggagcg ggtatctgtt ctttttgata ggatcaggaa aggctttcct
     9001 tgtgaagcca gagtggtggc caggatcctg ccccagtttc tagacgactt cttcccaccc
     9061 caggacatca tgaacaaagt catcggagag tttctgtcca accagcagcc atacccccag
     9121 ttcatggcca ccgtggtgta taaggtgttt cagactctgc acagcaccgg gcagtcgtcc
     9181 atggtccggg actgggtcat gctgtccctc tccaacttca cgcagagggc cccggtcgcc
     9241 atggccacgt ggagcctctc ctgcttcttt gtcagcgcgt ccaccagccc gtgggtcgcg
     9301 gcgatcctcc cacatgtcat cagcaggatg ggcaagctgg agcaggtgga cgtgaacctt
     9361 ttctgcctgg tcgccacaga cttctacaga caccagatag aggaggagct cgaccgcagg
     9421 gccttccagt ctgtgcttga ggtggttgca gccccaggaa gcccatatca ccggctgctg
     9481 acttgtttac gaaatgtcca caaggtcacc acctgc
//
