LOCUS       sequence-209887-        9618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8936..8945
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      241 caacagcaac aacagcagca acagcaacaa caacagcagc aacagcaaca acagcagcaa
      301 cagcaaccgc caccgccgcc gccgccgccg ccgcctcctc agcttcctca gccgccgccg
      361 caggcacagc cgctgctgcc tcagccgcag ccgcccccgc cgccgccccc gccgccaccc
      421 ggcccggctg tggctgagga gccgctgcac cgaccaaaga aagaactttc agctaccaag
      481 aaagaccgtg tgaatcattg tctgacaata tgtgaaaaca tagtggcaca gtctgtcaga
      541 aattctccag aatttcagaa acttctgggc atcgctatgg aactttttct gctgtgcagt
      601 gatgacgcag agtcagatgt caggatggtg gctgacgaat gcctcaacaa agttatcaaa
      661 gctttgatgg attctaatct tccaaggtta cagctcgagc tctataagga aattaaaaag
      721 aatggtgccc ctcggagttt gcgtgctgcc ctgtggaggt ttgctgagct ggctcacctg
      781 gttcggcctc agaaatgcag gccttacctg gtgaaccttc tgccgtgcct gactcgaaca
      841 agcaagagac ccgaagaatc agtccaggag accttggctg cagctgttcc caaaattatg
      901 gcttcttttg gcaattttgc aaatgacaat gaaattaagg ttttgttaaa ggccttcata
      961 gcgaacctga agtcaagctc ccccaccatt cggcggacag cggctggatc agcagtgagc
     1021 atctgccagc actcaagaag gacacaatat ttctatagtt ggctactaaa tgtgctctta
     1081 ggcttactcg ttcctgtcga ggatgaacac tccactctgc tgattcttgg cgtgctgctc
     1141 accctgaggt atttggtgcc cttgctgcag cagcaggtca aggacacaag cctgaaaggc
     1201 agcttcggag tgacaaggaa agaaatggaa gtctctcctt ctgcagagca gcttgtccag
     1261 gtttatgaac tgacgttaca tcatacacag caccaagacc acaatgttgt gaccggagcc
     1321 ctggagctgt tgcagcagct cttcagaacg cctccacccg agcttctgca aaccctgacc
     1381 gcagtcgggg gcattgggca gctcaccgct gctaaggagg agtctggtgg ccgaagccgt
     1441 agtgggagta ttgtggaact tatagctgga gggggttcct catgcagccc tgtcctttca
     1501 agaaaacaaa aaggcaaagt gctcttagga gaagaagaag ccttggagga tgactctgaa
     1561 tcgagatcgg atgtcagcag ctctgcctta acagcctcag tgaaggatga gatcagtgga
     1621 gagctggctg cttcttcagg ggtttccact ccagggtcag caggtcatga catcatcaca
     1681 gaacagccac ggtcacagca cacactgcag gcggactcag tggatctggc cagctgtgac
     1741 ttgacaagct ctgccactga tggggatgag gaggatatct tgagccacag ctccagccag
     1801 gtcagcgccg tcccatctga ccctgccatg gacctgaatg atgggaccca ggcctcgtcg
     1861 cccatcagcg acagctccca gaccaccacc gaagggcctg attcagctgt taccccttca
     1921 gacagttctg aaattgtgtt agacggtacc gacaaccagt atttgggcct gcagattgga
     1981 cagccccagg atgaagatga ggaagccaca ggtattcttc ctgatgaagc ctcggaggcc
     2041 ttcaggaact cttccatggc ccttcaacag gcacatttat tgaaaaacat gagtcactgc
     2101 aggcagcctt ctgacagcag tgttgataaa tttgtgttga gagatgaagc tactgaaccg
     2161 ggtgatcaag aaaacaagcc ttgccgcatc aaaggtgaca ttggacagtc cactgatgat
     2221 gactctgcac ctcttgtcca ttgtgtccgc cttttatctg cttcgttttt gctaacaggg
     2281 ggaaaaaatg tgctggttcc ggacagggat gtgagggtca gcgtgaaggc cctggccctc
     2341 agctgtgtgg gagcagctgt ggccctccac ccggaatctt tcttcagcaa actctataaa
     2401 gttcctcttg acaccacgga ataccctgag gaacagtatg tctcagacat cttgaactac
     2461 atcgatcatg gagacccaca ggttcgagga gccactgcca ttctctgtgg gaccctcatc
     2521 tgctccatcc tcagcaggtc ccgcttccac gtgggagatt ggatgggcac cattagaacc
     2581 ctcacaggaa atacattttc tttggcggat tgcattcctt tgctgcggaa aacactgaag
     2641 gatgagtctt ctgttacttg caagttagct tgtacagctg tgaggaactg tgtcatgagt
     2701 ctctgcagca gcagctacag tgagttagga ctgcagctga tcatcgatgt gctgactctg
     2761 aggaacagtt cctattggct ggtgaggaca gagcttctgg aaacccttgc agagattgac
     2821 ttcaggctgg tgagcttttt ggaggcaaaa gcagaaaact tacacagagg ggctcatcat
     2881 tatacagggc ttttaaaact gcaagaacga gtgctcaata atgttgtcat ccatttgctt
     2941 ggagatgaag accccagggt gcgacatgtt gccgcagcat cactaattag gcttgtccca
     3001 aagctgtttt ataaatgtga ccaaggacaa gctgatccag tagtggccgt ggcaagagat
     3061 caaagcagtg tttacctgaa acttctcatg catgagacgc agcctccatc tcatttctcc
     3121 gtcagcacaa taaccagaat atatagaggc tataacctac taccaagcat aacagacgtc
     3181 actatggaaa ataacctttc aagagttatt gcagcagttt ctcatgaact aatcacatca
     3241 accaccagag cactcacatt tggatgctgt gaagctttgt gtcttctttc cactgccttc
     3301 ccagtttgca tttggagttt aggttggcac tgtggagtgc ctccactgag tgcctcagat
     3361 gagtctagga agagctgtac cgttgggatg gccacaatga ttctgaccct gctctcgtca
     3421 gcttggttcc cattggatct ctcagcccat caagatgctt tgattttggc cggaaacttg
     3481 cttgcagcca gtgctcccaa atctctgaga agttcatggg cctctgaaga agaagccaac
     3541 ccagcagcca ccaagcaaga ggaggtctgg ccagccctgg gggaccgggc cctggtgccc
     3601 atggtggagc agctcttctc tcacctgctg aaggtgatta acatttgtgc ccacgtcctg
     3661 gatgacgtgg ctcctggacc cgcaataaag gcagccttgc cttctctaac aaacccccct
     3721 tctctaagtc ccatccgacg aaaggggaag gagaaagaac caggagaaca agcatctgta
     3781 ccgttgagtc ccaagaaagg cagtgaggcc agtgcagctt ctagacaatc tgatacctca
     3841 ggtcctgtta caacaagtaa atcctcatca ctggggagtt tctatcatct tccttcatac
     3901 ctcaaactgc atgatgtcct gaaagctaca cacgctaact acaaggtcac gctggatctt
     3961 cagaacagca cggaaaagtt tggagggttt cttcgctcag ccttggatgt tctttctcag
     4021 atactagagc tggccacact gcaggacatt gggaagtgtg ttgaagagat cctaggatac
     4081 ctgaaatcct gctttagtcg agaaccaatg atggcaactg tttgtgttca acaattgttg
     4141 aagactctct ttggcacaaa cttggcctcc cagtttgatg gcttatcttc caaccccagc
     4201 aagtcacaag gccgagcaca gcgccttggc tcctccagtg tgaggccagg cttgtaccac
     4261 tactgcttca tggccccgta cacccacttc acccaggccc tcgctgacgc cagcctgagg
     4321 aacatggtgc aggcggagca ggagaacgac acctcgggat ggtttgatgt cctccagaaa
     4381 gtgtctaccc agttgaagac aaacctcacg agtgtcacaa agaaccgtgc agataagaat
     4441 gctattcata atcacattcg tttgtttgaa cctcttgtta taaaagcttt aaaacagtac
     4501 acgactacaa catgtgtgca gttacagaag caggttttag atttgctggc gcagctggtt
     4561 cagttacggg ttaattactg tcttctggat tcagatcagg tgtttattgg ctttgtattg
     4621 aaacagtttg aatacattga agtgggccag ttcagggaat cagaggcaat cattccaaac
     4681 atctttttct tcttggtatt actatcttat gaacgctatc attcaaaaca gatcattgga
     4741 attcctaaaa tcattcagct ctgtgatggc atcatggcca gtggaaggaa ggctgtgaca
     4801 catgccatac cggctctgca gcccatagtc cacgacctct ttgtattaag aggaacaaat
     4861 aaagctgatg caggaaaaga gcttgaaacc caaaaagagg tggtggtgtc aatgttactg
     4921 agactcatcc agtaccatca ggtgttggag atgttcattc ttgtcctgca gcagtgccac
     4981 aaggagaatg aagacaagtg gaagcgactg tctcgacaga tagctgacat catcctccca
     5041 atgttagcca aacagcagat gcacattgac tctcatgaag cccttggagt gttaaataca
     5101 ttatttgaga ttttggcccc ttcctccctc cgtccggtag acatgctttt acggagtatg
     5161 ttcgtcactc caaacacaat ggcgtccgtg agcactgttc aactgtggat atcgggaatt
     5221 ctggccattt tgagggttct gatttcccag tcaactgaag atattgttct ttctcgtatt
     5281 caggagctct ccttctctcc gtatttaatc tcctgtacag taattaatag gttaagagat
     5341 ggggacagta cttcaacgct agaagaacac agtgaaggga aacaaataaa gaatttgcca
     5401 gaagaaacat tttcaaggtt tctattacaa ctggttggta ttcttttaga agacattgtt
     5461 acaaaacagc tgaaggtgga aatgagtgag cagcaacata ctttctattg ccaggaacta
     5521 ggcacactgc taatgtgtct gatccacatc ttcaagtctg gaatgttccg gagaatcaca
     5581 gcagctgcca ctaggctgtt ccgcagtgat ggctgtggcg gcagtttcta caccctggac
     5641 agcttgaact tgcgggctcg ttccatgatc accacccacc cggccctggt gctgctctgg
     5701 tgtcagatac tgctgcttgt caaccacacc gactaccgct ggtgggcaga agtgcagcag
     5761 accccgaaaa gacacagtct gtccagcaca aagttactta gtccccagat gtctggagaa
     5821 gaggaggatt ctgacttggc agccaaactt ggaatgtgca atagagaaat agtacgaaga
     5881 ggggctctca ttctcttctg tgattatgtc tgtcagaacc tccatgactc cgagcactta
     5941 acgtggctca ttgtaaatca cattcaagat ctgatcagcc tttcccacga gcctccagta
     6001 caggacttca tcagtgccgt tcatcggaac tctgctgcca gcggcctgtt catccaggca
     6061 attcagtctc gttgtgaaaa cctttcaact ccaaccatgc tgaagaaaac tcttcagtgc
     6121 ttggagggga tccatctcag ccagtcggga gctgtgctca cgctgtatgt ggacaggctt
     6181 ctgtgcaccc ctttccgtgt gctggctcgc atggtcgaca tccttgcttg tcgccgggta
     6241 gaaatgcttc tggctgcaaa tttacagagc agcatggccc agttgccaat ggaagaactc
     6301 aacagaatcc aggaatacct tcagagcagc gggctcgctc agagacacca aaggctctat
     6361 tccctgctgg acaggtttcg tctctccacc atgcaagact cacttagtcc ctctcctcca
     6421 gtctcttccc acccgctgga cggggatggg cacgtgtcac tggaaacagt gagtccggac
     6481 aaagactggt acgttcatct tgtcaaatcc cagtgttgga ccaggtcaga ttctgcactg
     6541 ctggaaggtg cagagctggt gaatcggatt cctgctgaag atatgaatgc cttcatgatg
     6601 aactcggagt tcaacctaag cctgctagct ccatgcttaa gcctagggat gagtgaaatt
     6661 tctggtggcc agaagagtgc cctttttgaa gcagcccgtg aggtgactct ggcccgtgtg
     6721 agcggcaccg tgcagcagct ccctgctgtc catcatgtct tccagcccga gctgcctgca
     6781 gaaccggcgg cctactggag caagttgaat gatctgtttg gggatgctgc actgtatcag
     6841 tccctgccca ctctggcccg ggccctggca cagtacctgg tggtggtctc caaactgccc
     6901 agtcatttgc accttcctcc tgagaaagag aaggacattg tgaaattcgt ggtggcaacc
     6961 cttgaggccc tgtcctggca tttgatccat gagcagatcc cgctgagtct ggatctccag
     7021 gcagggctgg actgctgctg cctggccctg cagctgcctg gcctctggag cgtggtctcc
     7081 tccacagagt ttgtgaccca cgcctgctcc ctcatccact gtgtgcactt catcctggag
     7141 gccgttgcag tgcagcctgg agagcagctt cttagtccag aaagaaggac aaatacccca
     7201 aaagccatca gcgaggagga ggaggaagta gatccaaaca cacagaatcc taagtatatc
     7261 actgcagcct gtgagatggt ggcagaaatg gtggagtctc tgcagtcggt gttggccttg
     7321 ggtcataaaa ggaatagcgg cgtgccggcg tttctcacgc cattgctcag gaacatcatc
     7381 atcagcctgg cccgcctgcc ccttgtcaac agctacacac gtgtgccccc actggtgtgg
     7441 aagcttggat ggtcacccaa accgggaggg gattttggca cagcattccc tgagatcccc
     7501 gtggagttcc tccaggaaaa ggaagtcttt aaggagttca tctaccgcat caacacacta
     7561 ggctggacca gtcgtactca gtttgaagaa acttgggcca ccctccttgg tgtcctggtg
     7621 acgcagcccc tcgtgatgga gcaggaggag agcccaccag aagaagacac agagaggacc
     7681 cagatcaacg tcctggccgt gcaggccatc acctcactgg tgctcagtgc aatgactgtg
     7741 cctgtggccg gcaacccagc tgtaagctgc ttggagcagc agccccggaa caagcctctg
     7801 aaagctctcg acaccaggtt tgggaggaag ctgagcatta tcagagggat tgtggagcaa
     7861 gagattcaag caatggtttc aaagagagag aatattgcca cccatcattt atatcaggca
     7921 tgggatcctg tcccttctct gtctccggct actacaggtg ccctcatcag ccacgagaag
     7981 ctgctgctgc agatcaaccc cgagcgggag ctggggagca tgagctacaa actcggccag
     8041 gtgtccatac actccgtgtg gctagggaac agcatcacac ccctgaggga ggaggaatgg
     8101 gacgaggaag aggaggagga ggccgacgcc cctgcacctt cgtcaccacc cacgtctcca
     8161 gtcaactcca ggaaacaccg ggctggagtt gacatccact cctgttcgca gtttttgctt
     8221 gagttgtaca gccgctggat cctgccgtcc agctcagcca ggaggacccc ggccatcctg
     8281 atcagtgagg tggtcagatc ccttctagtg gtctcagact tgttcaccga gcgcaaccag
     8341 tttgagctga tgtatgtgac gctgacagaa ctgcgaaggg tgcacccttc agaagacgag
     8401 atcctcgctc agtacctggt gcctgccacc tgcaaggcag ctgccgtcct tgggatggac
     8461 aaggccgtgg cggagcctgt cagccgcctg ctggagagca cgctcaggag cagccacctg
     8521 cccagcaggg ttggagccct gcacggcatc ctctatgtgc tggagtgcga cctgctggac
     8581 gacactgcca agcagctcat cccggtcatc agcgactatc tcctctccaa cctgaaaggg
     8641 atcgcccact gcgtgaacat tcacagccag cagcacgtac tggtcatgtg tgccactgcg
     8701 ttttacctca ttgagaacta tcctctggac gtagggccgg aattttcagc atcaataata
     8761 cagatgtgtg gggtgatgct gtctggaagt gaggagtcca ccccctccat catttaccac
     8821 tgtgccctca gaggcctgga gcgcctcctg ctctctgagc agctctcccg cctggatgca
     8881 gaatcgctgg tcaagctgag tgtggacaga gtgaacgtgc acagcccgca ccgggccatg
     8941 gcggctctgg gcctgatgct cacctgcatg tacacaggaa aggagaaagt cagtccgggt
     9001 agaacttcag accctaatcc tgcagccccc gacagcgagt cagtgattgt tgctatggag
     9061 cgggtatctg ttctttttga taggatcagg aaaggctttc cttgtgaagc cagagtggtg
     9121 gccaggatcc tgccccagtt tctagacgac ttcttcccac cccaggacat catgaacaaa
     9181 gtcatcggag agtttctgtc caaccagcag ccataccccc agttcatggc caccgtggtg
     9241 tataaggtgt ttcagactct gcacagcacc gggcagtcgt ccatggtccg ggactgggtc
     9301 atgctgtccc tctccaactt cacgcagagg gccccggtcg ccatggccac gtggagcctc
     9361 tcctgcttct ttgtcagcgc gtccaccagc ccgtgggtcg cggcgatcct cccacatgtc
     9421 atcagcagga tgggcaagct ggagcaggtg gacgtgaacc ttttctgcct ggtcgccaca
     9481 gacttctaca gacaccagat agaggaggag ctcgaccgca gggccttcca gtctgtgctt
     9541 gaggtggttg cagccccagg aagcccatat caccggctgc tgacttgttt acgaaatgtc
     9601 cacaaggtca ccacctgc
//
