LOCUS       sequence-209888-        9597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8915..8924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      241 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaaccgcc accgccgccg
      301 ccgccgccgc cgcctcctca gcttcctcag ccgccgccgc aggcacagcc cctgctgcct
      361 cagccgcagc cgcccccgcc gccgcccccg ccgccacccg gcccggctgt ggctgaggag
      421 ccgctgcacc gaccaaagaa agaactttca gctaccaaga aagaccgtgt gaatcattgt
      481 ctgacaatat gtgaaaacat agtggcacag tctgtcagaa attctccaga atttcagaaa
      541 cttctgggca tcgctatgga actttttctg ctgtgcagtg atgacgcaga gtcagatgtc
      601 aggatggtgg ctgacgaatg cctcaacaaa gttatcaaag ctttgatgga ttctaatctt
      661 ccaaggttac agctcgagct ctataaggaa attaaaaaga atggtgcccc tcggagtttg
      721 cgtgctgccc tgtggaggtt tgctgagctg gctcacctgg ttcggcctca gaaatgcagg
      781 ccttacctgg tgaaccttct gccgtgcctg actcgaacaa gcaagagacc cgaagaatca
      841 gtccaggaga ccttggctgc agctgttccc aaaattatgg cttcttttgg caattttgca
      901 aatgacaatg aaattaaggt tttgttaaag gccttcatag cgaacctgaa gtcaagctcc
      961 cccaccattc ggcggacagc ggctggatca gcagtgagca tctgccagca ctcaagaagg
     1021 acacaatatt tctatagttg gctactaaat gtgctcttag gcttactcgt tcctgtcgag
     1081 gatgaacact ccactctgct gattcttggc gtgctgctca ccctgaggta tttggtgccc
     1141 ttgctgcagc agcaggtcaa ggacacaagc ctgaaaggca gcttcggagt gacaaggaaa
     1201 gaaatggaag tctctccttc tgcagagcag cttgtccagg tttatgaact gacgttacat
     1261 catacacagc accaagacca caatgttgtg accggagccc tggagctgtt gcagcagctc
     1321 ttcagaacgc ctccacccga gcttctgcaa accctgaccg cagtcggggg cattgggcag
     1381 ctcaccgctg ctaaggagga gtctggtggc cgaagccgta gtgggagtat tgtggaactt
     1441 atagctggag ggggttcctc atgcagccct gtcctttcaa gaaaacaaaa aggcaaagtg
     1501 ctcttaggag aagaagaagc cttggaggat gactctgaat cgagatcgga tgtcagcagc
     1561 tctgccttaa cagcctcagt gaaggatgag atcagtggag agctggctgc ttcttcaggg
     1621 gtttccactc cagggtcagc aggtcatgac atcatcacag aacagccacg gtcacagcac
     1681 acactgcagg cggactcagt ggatctggcc agctgtgact tgacaagctc tgccactgat
     1741 ggggatgagg aggatatctt gagccacagc tccagccagg tcagcgccgt cccatctgac
     1801 cctgccatgg acctgaatga tgggacccag gcctcgtcgc ccatcagcga cagctcccag
     1861 accaccaccg aagggcctga ttcagctgtt accccttcag acagttctga aattgtgtta
     1921 gacggtaccg acaaccagta tttgggcctg cagattggac agccccagga tgaagatgag
     1981 gaagccacag gtattcttcc tgatgaagcc tcggaggcct tcaggaactc ttccatggcc
     2041 cttcaacagg cacatttatt gaaaaacatg agtcactgca ggcagccttc tgacagcagt
     2101 gttgataaat ttgtgttgag agatgaagct actgaaccgg gtgatcaaga aaacaagcct
     2161 tgccgcatca aaggtgacat tggacagtcc actgatgatg actctgcacc tcttgtccat
     2221 tgtgtccgcc ttttatctgc ttcgtttttg ctaacagggg gaaaaaatgt gctggttccg
     2281 gacagggatg tgagggtcag cgtgaaggcc ctggccctca gctgtgtggg agcagctgtg
     2341 gccctccacc cggaatcttt cttcagcaaa ctctataaag ttcctcttga caccacggaa
     2401 taccctgagg aacagtatgt ctcagacatc ttgaactaca tcgatcatgg agacccacag
     2461 gttcgaggag ccactgccat tctctgtggg accctcatct gctccatcct cagcaggtcc
     2521 cgcttccacg tgggagattg gatgggcacc attagaaccc tcacaggaaa tacattttct
     2581 ttggcggatt gcattccttt gctgcggaaa acactgaagg atgagtcttc tgttacttgc
     2641 aagttagctt gtacagctgt gaggaactgt gtcatgagtc tctgcagcag cagctacagt
     2701 gagttaggac tgcagctgat catcgatgtg ctgactctga ggaacagttc ctattggctg
     2761 gtgaggacag agcttctgga aacccttgca gagattgact tcaggctggt gagctttttg
     2821 gaggcaaaag cagaaaactt acacagaggg gctcatcatt atacagggct tttaaaactg
     2881 caagaacgag tgctcaataa tgttgtcatc catttgcttg gagatgaaga ccccagggtg
     2941 cgacatgttg ccgcagcatc actaattagg cttgtcccaa agctgtttta taaatgtgac
     3001 caaggacaag ctgatccagt agtggccgtg gcaagagatc aaagcagtgt ttacctgaaa
     3061 cttctcatgc atgagacgca gcctccatct catttctccg tcagcacaat aaccagaata
     3121 tatagaggct ataacctact accaagcata acagacgtca ctatggaaaa taacctttca
     3181 agagttattg cagcagtttc tcatgaacta atcacatcaa ccaccagagc actcacattt
     3241 ggatgctgtg aagctttgtg tcttctttcc actgccttcc cagtttgcat ttggagttta
     3301 ggttggcact gtggagtgcc tccactgagt gcctcagatg agtctaggaa gagctgtacc
     3361 gttgggatgg ccacaatgat tctgaccctg ctctcgtcag cttggttccc attggatctc
     3421 tcagcccatc aagatgcttt gattttggcc ggaaacttgc ttgcagccag tgctcccaaa
     3481 tctctgagaa gttcatgggc ctctgaagaa gaagccaacc cagcagccac caagcaagag
     3541 gaggtctggc cagccctggg ggaccgggcc ctggtgccca tggtggagca gctcttctct
     3601 cacctgctga aggtgattaa catttgtgcc cacgtcctgg atgacgtggc tcctggaccc
     3661 gcaataaagg cagccttgcc ttctctaaca aacccccctt ctctaagtcc catccgacga
     3721 aaggggaagg agaaagaacc aggagaacaa gcatctgtac cgttgagtcc caagaaaggc
     3781 agtgaggcca gtgcagcttc tagacaatct gatacctcag gtcctgttac aacaagtaaa
     3841 tcctcatcac tggggagttt ctatcatctt ccttcatacc tcaaactgca tgatgtcctg
     3901 aaagctacac acgctaacta caaggtcacg ctggatcttc agaacagcac ggaaaagttt
     3961 ggagggtttc ttcgctcagc cttggatgtt ctttctcaga tactagagct ggccacactg
     4021 caggacattg ggaagtgtgt tgaagagatc ctaggatacc tgaaatcctg ctttagtcga
     4081 gaaccaatga tggcaactgt ttgtgttcaa caattgttga agactctctt tggcacaaac
     4141 ttggcctccc agtttgatgg cttatcttcc aaccccagca agtcacaagg ccgagcacag
     4201 cgccttggct cctccagtgt gaggccaggc ttgtaccact actgcttcat ggccccgtac
     4261 acccacttca cccaggccct cgctgacgcc agcctgagga acatggtgca ggcggagcag
     4321 gagaacgaca cctcgggatg gtttgatgtc ctccagaaag tgtctaccca gttgaagaca
     4381 aacctcacga gtgtcacaaa gaaccgtgca gataagaatg ctattcataa tcacattcgt
     4441 ttgtttgaac ctcttgttat aaaagcttta aaacagtaca cgactacaac atgtgtgcag
     4501 ttacagaagc aggttttaga tttgctggcg cagctggttc agttacgggt taattactgt
     4561 cttctggatt cagatcaggt gtttattggc tttgtattga aacagtttga atacattgaa
     4621 gtgggccagt tcagggaatc agaggcaatc attccaaaca tctttttctt cttggtatta
     4681 ctatcttatg aacgctatca ttcaaaacag atcattggaa ttcctaaaat cattcagctc
     4741 tgtgatggca tcatggccag tggaaggaag gctgtgacac atgccatacc ggctctgcag
     4801 cccatagtcc acgacctctt tgtattaaga ggaacaaata aagctgatgc aggaaaagag
     4861 cttgaaaccc aaaaagaggt ggtggtgtca atgttactga gactcatcca gtaccatcag
     4921 gtgttggaga tgttcattct tgtcctgcag cagtgccaca aggagaatga agacaagtgg
     4981 aagcgactgt ctcgacagat agctgacatc atcctcccaa tgttagccaa acagcagatg
     5041 cacattgact ctcatgaagc ccttggagtg ttaaatacat tatttgagat tttggcccct
     5101 tcctccctcc gtccggtaga catgctttta cggagtatgt tcgtcactcc aaacacaatg
     5161 gcgtccgtga gcactgttca actgtggata tcgggaattc tggccatttt gagggttctg
     5221 atttcccagt caactgaaga tattgttctt tctcgtattc aggagctctc cttctctccg
     5281 tatttaatct cctgtacagt aattaatagg ttaagagatg gggacagtac ttcaacgcta
     5341 gaagaacaca gtgaagggaa acaaataaag aatttgccag aagaaacatt ttcaaggttt
     5401 ctattacaac tggttggtat tcttttagaa gacattgtta caaaacagct gaaggtggaa
     5461 atgagtgagc agcaacatac tttctattgc caggaactag gcacactgct aatgtgtctg
     5521 atccacatct tcaagtctgg aatgttccgg agaatcacag cagctgccac taggctgttc
     5581 cgcagtgatg gctgtggcgg cagtttctac accctggaca gcttgaactt gcgggctcgt
     5641 tccatgatca ccacccaccc ggccctggtg ctgctctggt gtcagatact gctgcttgtc
     5701 aaccacaccg actaccgctg gtgggcagaa gtgcagcaga ccccgaaaag acacagtctg
     5761 tccagcacaa agttacttag tccccagatg tctggagaag aggaggattc tgacttggca
     5821 gccaaacttg gaatgtgcaa tagagaaata gtacgaagag gggctctcat tctcttctgt
     5881 gattatgtct gtcagaacct ccatgactcc gagcacttaa cgtggctcat tgtaaatcac
     5941 attcaagatc tgatcagcct ttcccacgag cctccagtac aggacttcat cagtgccgtt
     6001 catcggaact ctgctgccag cggcctgttc atccaggcaa ttcagtctcg ttgtgaaaac
     6061 ctttcaactc caaccatgct gaagaaaact cttcagtgct tggaggggat ccatctcagc
     6121 cagtcgggag ctgtgctcac gctgtatgtg gacaggcttc tgtgcacccc tttccgtgtg
     6181 ctggctcgca tggtcgacat ccttgcttgt cgccgggtag aaatgcttct ggctgcaaat
     6241 ttacagagca gcatggccca gttgccaatg gaagaactca acagaatcca ggaatacctt
     6301 cagagcagcg ggctcgctca gagacaccaa aggctctatt ccctgctgga caggtttcgt
     6361 ctctccacca tgcaagactc acttagtccc tctcctccag tctcttccca cccgctggac
     6421 ggggatgggc acgtgtcact ggaaacagtg agtccggaca aagactggta cgttcatctt
     6481 gtcaaatccc agtgttggac caggtcagat tctgcactgc tggaaggtgc agagctggtg
     6541 aatcggattc ctgctgaaga tatgaatgcc ttcatgatga actcggagtt caacctaagc
     6601 ctgctagctc catgcttaag cctagggatg agtgaaattt ctggtggcca gaagagtgcc
     6661 ctttttgaag cagcccgtga ggtgactctg gcccgtgtga gcggcaccgt gcagcagctc
     6721 cctgctgtcc atcatgtctt ccagcccgag ctgcctgcag aaccggcggc ctactggagc
     6781 aagttgaatg atctgtttgg ggatgctgca ctgtatcagt ccctgcccac tctggcccgg
     6841 gccctggcac agtacctggt ggtggtctcc aaactgccca gtcatttgca ccttcctcct
     6901 gagaaagaga aggacattgt gaaattcgtg gtggcaaccc ttgaggccct gtcctggcat
     6961 ttgatccatg agcagatccc gctgagtctg gatctccagg cagggctgga ctgctgctgc
     7021 ctggccctgc agctgcctgg cctctggagc gtggtctcct ccacagagtt tgtgacccac
     7081 gcctgctccc tcatccactg tgtgcacttc atcctggagg ccgttgcagt gcagcctgga
     7141 gagcagcttc ttagtccaga aagaaggaca aataccccaa aagccatcag cgaggaggag
     7201 gaggaagtag atccaaacac acagaatcct aagtatatca ctgcagcctg tgagatggtg
     7261 gcagaaatgg tggagtctct gcagtcggtg ttggccttgg gtcataaaag gaatagcggc
     7321 gtgccggcgt ttctcacgcc attgctcagg aacatcatca tcagcctggc ccgcctgccc
     7381 cttgtcaaca gctacacacg tgtgccccca ctggtgtgga agcttggatg gtcacccaaa
     7441 ccgggagggg attttggcac agcattccct gagatccccg tggagttcct ccaggaaaag
     7501 gaagtcttta aggagttcat ctaccgcatc aacacactag gctggaccag tcgtactcag
     7561 tttgaagaaa cttgggccac cctccttggt gtcctggtga cgcagcccct cgtgatggag
     7621 caggaggaga gcccaccaga agaagacaca gagaggaccc agatcaacgt cctggccgtg
     7681 caggccatca cctcactggt gctcagtgca atgactgtgc ctgtggccgg caacccagct
     7741 gtaagctgct tggagcagca gccccggaac aagcctctga aagctctcga caccaggttt
     7801 gggaggaagc tgagcattat cagagggatt gtggagcaag agattcaagc aatggtttca
     7861 aagagagaga atattgccac ccatcattta tatcaggcat gggatcctgt cccttctctg
     7921 tctccggcta ctacaggtgc cctcatcagc cacgagaagc tgctgctgca gatcaacccc
     7981 gagcgggagc tggggagcat gagctacaaa ctcggccagg tgtccataca ctccgtgtgg
     8041 ctagggaaca gcatcacacc cctgagggag gaggaatggg acgaggaaga ggaggaggag
     8101 gccgacgccc ctgcaccttc gtcaccaccc acgtctccag tcaactccag gaaacaccgg
     8161 gctggagttg acatccactc ctgttcgcag tttttgcttg agttgtacag ccgctggatc
     8221 ctgccgtcca gctcagccag gaggaccccg gccatcctga tcagtgaggt ggtcagatcc
     8281 cttctagtgg tctcagactt gttcaccgag cgcaaccagt ttgagctgat gtatgtgacg
     8341 ctgacagaac tgcgaagggt gcacccttca gaagacgaga tcctcgctca gtacctggtg
     8401 cctgccacct gcaaggcagc tgccgtcctt gggatggaca aggccgtggc ggagcctgtc
     8461 agccgcctgc tggagagcac gctcaggagc agccacctgc ccagcagggt tggagccctg
     8521 cacggcatcc tctatgtgct ggagtgcgac ctgctggacg acactgccaa gcagctcatc
     8581 ccggtcatca gcgactatct cctctccaac ctgaaaggga tcgcccactg cgtgaacatt
     8641 cacagccagc agcacgtact ggtcatgtgt gccactgcgt tttacctcat tgagaactat
     8701 cctctggacg tagggccgga attttcagca tcaataatac agatgtgtgg ggtgatgctg
     8761 tctggaagtg aggagtccac cccctccatc atttaccact gtgccctcag aggcctggag
     8821 cgcctcctgc tctctgagca gctctcccgc ctggatgcag aatcgctggt caagctgagt
     8881 gtggacagag tgaacgtgca cagcccgcac cgggccatgg cggctctggg cctgatgctc
     8941 acctgcatgt acacaggaaa ggagaaagtc agtccgggta gaacttcaga ccctaatcct
     9001 gcagcccccg acagcgagtc agtgattgtt gctatggagc gggtatctgt tctttttgat
     9061 aggatcagga aaggctttcc ttgtgaagcc agagtggtgg ccaggatcct gccccagttt
     9121 ctagacgact tcttcccacc ccaggacatc atgaacaaag tcatcggaga gtttctgtcc
     9181 aaccagcagc cataccccca gttcatggcc accgtggtgt ataaggtgtt tcagactctg
     9241 cacagcaccg ggcagtcgtc catggtccgg gactgggtca tgctgtccct ctccaacttc
     9301 acgcagaggg ccccggtcgc catggccacg tggagcctct cctgcttctt tgtcagcgcg
     9361 tccaccagcc cgtgggtcgc ggcgatcctc ccacatgtca tcagcaggat gggcaagctg
     9421 gagcaggtgg acgtgaacct tttctgcctg gtcgccacag acttctacag acaccagata
     9481 gaggaggagc tcgaccgcag ggccttccag tctgtgcttg aggtggttgc agccccagga
     9541 agcccatatc accggctgct gacttgttta cgaaatgtcc acaaggtcac cacctgc
//
