LOCUS       sequence-209889-        9780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9098..9107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      241 caacagcaac aacagcagca acagcaacaa caacagcagc aacagcaaca acagcagcaa
      301 cagcaacagc agcagcaaca gcaacaacag cagcaacagc aacaacagca gcaacagcaa
      361 caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca gcaacaacag
      421 cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaacc gccaccgccg
      481 ccgccgccgc cgccgcctcc tcagcttcct cagccgccgc cgcaggcaca gccgctgctg
      541 cctcagccgc agccgccccc gccgccgccc ccgccgccac ccggcccggc tgtggctgag
      601 gagccgctgc accgaccaaa gaaagaactt tcagctacca agaaagaccg tgtgaatcat
      661 tgtctgacaa tatgtgaaaa catagtggca cagtctgtca gaaattctcc agaatttcag
      721 aaacttctgg gcatcgctat ggaacttttt ctgctgtgca gtgatgacgc agagtcagat
      781 gtcaggatgg tggctgacga atgcctcaac aaagttatca aagctttgat ggattctaat
      841 cttccaaggt tacagctcga gctctataag gaaattaaaa agaatggtgc ccctcggagt
      901 ttgcgtgctg ccctgtggag gtttgctgag ctggctcacc tggttcggcc tcagaaatgc
      961 aggccttacc tggtgaacct tctgccgtgc ctgactcgaa caagcaagag acccgaagaa
     1021 tcagtccagg agaccttggc tgcagctgtt cccaaaatta tggcttcttt tggcaatttt
     1081 gcaaatgaca atgaaattaa ggttttgtta aaggccttca tagcgaacct gaagtcaagc
     1141 tcccccacca ttcggcggac agcggctgga tcagcagtga gcatctgcca gcactcaaga
     1201 aggacacaat atttctatag ttggctacta aatgtgctct taggcttact cgttcctgtc
     1261 gaggatgaac actccactct gctgattctt ggcgtgctgc tcaccctgag gtatttggtg
     1321 cccttgctgc agcagcaggt caaggacaca agcctgaaag gcagcttcgg agtgacaagg
     1381 aaagaaatgg aagtctctcc ttctgcagag cagcttgtcc aggtttatga actgacgtta
     1441 catcatacac agcaccaaga ccacaatgtt gtgaccggag ccctggagct gttgcagcag
     1501 ctcttcagaa cgcctccacc cgagcttctg caaaccctga ccgcagtcgg gggcattggg
     1561 cagctcaccg ctgctaagga ggagtctggt ggccgaagcc gtagtgggag tattgtggaa
     1621 cttatagctg gagggggttc ctcatgcagc cctgtccttt caagaaaaca aaaaggcaaa
     1681 gtgctcttag gagaagaaga agccttggag gatgactctg aatcgagatc ggatgtcagc
     1741 agctctgcct taacagcctc agtgaaggat gagatcagtg gagagctggc tgcttcttca
     1801 ggggtttcca ctccagggtc agcaggtcat gacatcatca cagaacagcc acggtcacag
     1861 cacacactgc aggcggactc agtggatctg gccagctgtg acttgacaag ctctgccact
     1921 gatggggatg aggaggatat cttgagccac agctccagcc aggtcagcgc cgtcccatct
     1981 gaccctgcca tggacctgaa tgatgggacc caggcctcgt cgcccatcag cgacagctcc
     2041 cagaccacca ccgaagggcc tgattcagct gttacccctt cagacagttc tgaaattgtg
     2101 ttagacggta ccgacaacca gtatttgggc ctgcagattg gacagcccca ggatgaagat
     2161 gaggaagcca caggtattct tcctgatgaa gcctcggagg ccttcaggaa ctcttccatg
     2221 gcccttcaac aggcacattt attgaaaaac atgagtcact gcaggcagcc ttctgacagc
     2281 agtgttgata aatttgtgtt gagagatgaa gctactgaac cgggtgatca agaaaacaag
     2341 ccttgccgca tcaaaggtga cattggacag tccactgatg atgactctgc acctcttgtc
     2401 cattgtgtcc gccttttatc tgcttcgttt ttgctaacag ggggaaaaaa tgtgctggtt
     2461 ccggacaggg atgtgagggt cagcgtgaag gccctggccc tcagctgtgt gggagcagct
     2521 gtggccctcc acccggaatc tttcttcagc aaactctata aagttcctct tgacaccacg
     2581 gaataccctg aggaacagta tgtctcagac atcttgaact acatcgatca tggagaccca
     2641 caggttcgag gagccactgc cattctctgt gggaccctca tctgctccat cctcagcagg
     2701 tcccgcttcc acgtgggaga ttggatgggc accattagaa ccctcacagg aaatacattt
     2761 tctttggcgg attgcattcc tttgctgcgg aaaacactga aggatgagtc ttctgttact
     2821 tgcaagttag cttgtacagc tgtgaggaac tgtgtcatga gtctctgcag cagcagctac
     2881 agtgagttag gactgcagct gatcatcgat gtgctgactc tgaggaacag ttcctattgg
     2941 ctggtgagga cagagcttct ggaaaccctt gcagagattg acttcaggct ggtgagcttt
     3001 ttggaggcaa aagcagaaaa cttacacaga ggggctcatc attatacagg gcttttaaaa
     3061 ctgcaagaac gagtgctcaa taatgttgtc atccatttgc ttggagatga agaccccagg
     3121 gtgcgacatg ttgccgcagc atcactaatt aggcttgtcc caaagctgtt ttataaatgt
     3181 gaccaaggac aagctgatcc agtagtggcc gtggcaagag atcaaagcag tgtttacctg
     3241 aaacttctca tgcatgagac gcagcctcca tctcatttct ccgtcagcac aataaccaga
     3301 atatatagag gctataacct actaccaagc ataacagacg tcactatgga aaataacctt
     3361 tcaagagtta ttgcagcagt ttctcatgaa ctaatcacat caaccaccag agcactcaca
     3421 tttggatgct gtgaagcttt gtgtcttctt tccactgcct tcccagtttg catttggagt
     3481 ttaggttggc actgtggagt gcctccactg agtgcctcag atgagtctag gaagagctgt
     3541 accgttggga tggccacaat gattctgacc ctgctctcgt cagcttggtt cccattggat
     3601 ctctcagccc atcaagatgc tttgattttg gccggaaact tgcttgcagc cagtgctccc
     3661 aaatctctga gaagttcatg ggcctctgaa gaagaagcca acccagcagc caccaagcaa
     3721 gaggaggtct ggccagccct gggggaccgg gccctggtgc ccatggtgga gcagctcttc
     3781 tctcacctgc tgaaggtgat taacatttgt gcccacgtcc tggatgacgt ggctcctgga
     3841 cccgcaataa aggcagcctt gccttctcta acaaaccccc cttctctaag tcccatccga
     3901 cgaaagggga aggagaaaga accaggagaa caagcatctg taccgttgag tcccaagaaa
     3961 ggcagtgagg ccagtgcagc ttctagacaa tctgatacct caggtcctgt tacaacaagt
     4021 aaatcctcat cactggggag tttctatcat cttccttcat acctcaaact gcatgatgtc
     4081 ctgaaagcta cacacgctaa ctacaaggtc acgctggatc ttcagaacag cacggaaaag
     4141 tttggagggt ttcttcgctc agccttggat gttctttctc agatactaga gctggccaca
     4201 ctgcaggaca ttgggaagtg tgttgaagag atcctaggat acctgaaatc ctgctttagt
     4261 cgagaaccaa tgatggcaac tgtttgtgtt caacaattgt tgaagactct ctttggcaca
     4321 aacttggcct cccagtttga tggcttatct tccaacccca gcaagtcaca aggccgagca
     4381 cagcgccttg gctcctccag tgtgaggcca ggcttgtacc actactgctt catggccccg
     4441 tacacccact tcacccaggc cctcgctgac gccagcctga ggaacatggt gcaggcggag
     4501 caggagaacg acacctcggg atggtttgat gtcctccaga aagtgtctac ccagttgaag
     4561 acaaacctca cgagtgtcac aaagaaccgt gcagataaga atgctattca taatcacatt
     4621 cgtttgtttg aacctcttgt tataaaagct ttaaaacagt acacgactac aacatgtgtg
     4681 cagttacaga agcaggtttt agatttgctg gcgcagctgg ttcagttacg ggttaattac
     4741 tgtcttctgg attcagatca ggtgtttatt ggctttgtat tgaaacagtt tgaatacatt
     4801 gaagtgggcc agttcaggga atcagaggca atcattccaa acatcttttt cttcttggta
     4861 ttactatctt atgaacgcta tcattcaaaa cagatcattg gaattcctaa aatcattcag
     4921 ctctgtgatg gcatcatggc cagtggaagg aaggctgtga cacatgccat accggctctg
     4981 cagcccatag tccacgacct ctttgtatta agaggaacaa ataaagctga tgcaggaaaa
     5041 gagcttgaaa cccaaaaaga ggtggtggtg tcaatgttac tgagactcat ccagtaccat
     5101 caggtgttgg agatgttcat tcttgtcctg cagcagtgcc acaaggagaa tgaagacaag
     5161 tggaagcgac tgtctcgaca gatagctgac atcatcctcc caatgttagc caaacagcag
     5221 atgcacattg actctcatga agcccttgga gtgttaaata cattatttga gattttggcc
     5281 ccttcctccc tccgtccggt agacatgctt ttacggagta tgttcgtcac tccaaacaca
     5341 atggcgtccg tgagcactgt tcaactgtgg atatcgggaa ttctggccat tttgagggtt
     5401 ctgatttccc agtcaactga agatattgtt ctttctcgta ttcaggagct ctccttctct
     5461 ccgtatttaa tctcctgtac agtaattaat aggttaagag atggggacag tacttcaacg
     5521 ctagaagaac acagtgaagg gaaacaaata aagaatttgc cagaagaaac attttcaagg
     5581 tttctattac aactggttgg tattctttta gaagacattg ttacaaaaca gctgaaggtg
     5641 gaaatgagtg agcagcaaca tactttctat tgccaggaac taggcacact gctaatgtgt
     5701 ctgatccaca tcttcaagtc tggaatgttc cggagaatca cagcagctgc cactaggctg
     5761 ttccgcagtg atggctgtgg cggcagtttc tacaccctgg acagcttgaa cttgcgggct
     5821 cgttccatga tcaccaccca cccggccctg gtgctgctct ggtgtcagat actgctgctt
     5881 gtcaaccaca ccgactaccg ctggtgggca gaagtgcagc agaccccgaa aagacacagt
     5941 ctgtccagca caaagttact tagtccccag atgtctggag aagaggagga ttctgacttg
     6001 gcagccaaac ttggaatgtg caatagagaa atagtacgaa gaggggctct cattctcttc
     6061 tgtgattatg tctgtcagaa cctccatgac tccgagcact taacgtggct cattgtaaat
     6121 cacattcaag atctgatcag cctttcccac gagcctccag tacaggactt catcagtgcc
     6181 gttcatcgga actctgctgc cagcggcctg ttcatccagg caattcagtc tcgttgtgaa
     6241 aacctttcaa ctccaaccat gctgaagaaa actcttcagt gcttggaggg gatccatctc
     6301 agccagtcgg gagctgtgct cacgctgtat gtggacaggc ttctgtgcac ccctttccgt
     6361 gtgctggctc gcatggtcga catccttgct tgtcgccggg tagaaatgct tctggctgca
     6421 aatttacaga gcagcatggc ccagttgcca atggaagaac tcaacagaat ccaggaatac
     6481 cttcagagca gcgggctcgc tcagagacac caaaggctct attccctgct ggacaggttt
     6541 cgtctctcca ccatgcaaga ctcacttagt ccctctcctc cagtctcttc ccacccgctg
     6601 gacggggatg ggcacgtgtc actggaaaca gtgagtccgg acaaagactg gtacgttcat
     6661 cttgtcaaat cccagtgttg gaccaggtca gattctgcac tgctggaagg tgcagagctg
     6721 gtgaatcgga ttcctgctga agatatgaat gccttcatga tgaactcgga gttcaaccta
     6781 agcctgctag ctccatgctt aagcctaggg atgagtgaaa tttctggtgg ccagaagagt
     6841 gccctttttg aagcagcccg tgaggtgact ctggcccgtg tgagcggcac cgtgcagcag
     6901 ctccctgctg tccatcatgt cttccagccc gagctgcctg cagaaccggc ggcctactgg
     6961 agcaagttga atgatctgtt tggggatgct gcactgtatc agtccctgcc cactctggcc
     7021 cgggccctgg cacagtacct ggtggtggtc tccaaactgc ccagtcattt gcaccttcct
     7081 cctgagaaag agaaggacat tgtgaaattc gtggtggcaa cccttgaggc cctgtcctgg
     7141 catttgatcc atgagcagat cccgctgagt ctggatctcc aggcagggct ggactgctgc
     7201 tgcctggccc tgcagctgcc tggcctctgg agcgtggtct cctccacaga gtttgtgacc
     7261 cacgcctgct ccctcatcca ctgtgtgcac ttcatcctgg aggccgttgc agtgcagcct
     7321 ggagagcagc ttcttagtcc agaaagaagg acaaataccc caaaagccat cagcgaggag
     7381 gaggaggaag tagatccaaa cacacagaat cctaagtata tcactgcagc ctgtgagatg
     7441 gtggcagaaa tggtggagtc tctgcagtcg gtgttggcct tgggtcataa aaggaatagc
     7501 ggcgtgccgg cgtttctcac gccattgctc aggaacatca tcatcagcct ggcccgcctg
     7561 ccccttgtca acagctacac acgtgtgccc ccactggtgt ggaagcttgg atggtcaccc
     7621 aaaccgggag gggattttgg cacagcattc cctgagatcc ccgtggagtt cctccaggaa
     7681 aaggaagtct ttaaggagtt catctaccgc atcaacacac taggctggac cagtcgtact
     7741 cagtttgaag aaacttgggc caccctcctt ggtgtcctgg tgacgcagcc cctcgtgatg
     7801 gagcaggagg agagcccacc agaagaagac acagagagga cccagatcaa cgtcctggcc
     7861 gtgcaggcca tcacctcact ggtgctcagt gcaatgactg tgcctgtggc cggcaaccca
     7921 gctgtaagct gcttggagca gcagccccgg aacaagcctc tgaaagctct cgacaccagg
     7981 tttgggagga agctgagcat tatcagaggg attgtggagc aagagattca agcaatggtt
     8041 tcaaagagag agaatattgc cacccatcat ttatatcagg catgggatcc tgtcccttct
     8101 ctgtctccgg ctactacagg tgccctcatc agccacgaga agctgctgct gcagatcaac
     8161 cccgagcggg agctggggag catgagctac aaactcggcc aggtgtccat acactccgtg
     8221 tggctaggga acagcatcac acccctgagg gaggaggaat gggacgagga agaggaggag
     8281 gaggccgacg cccctgcacc ttcgtcacca cccacgtctc cagtcaactc caggaaacac
     8341 cgggctggag ttgacatcca ctcctgttcg cagtttttgc ttgagttgta cagccgctgg
     8401 atcctgccgt ccagctcagc caggaggacc ccggccatcc tgatcagtga ggtggtcaga
     8461 tcccttctag tggtctcaga cttgttcacc gagcgcaacc agtttgagct gatgtatgtg
     8521 acgctgacag aactgcgaag ggtgcaccct tcagaagacg agatcctcgc tcagtacctg
     8581 gtgcctgcca cctgcaaggc agctgccgtc cttgggatgg acaaggccgt ggcggagcct
     8641 gtcagccgcc tgctggagag cacgctcagg agcagccacc tgcccagcag ggttggagcc
     8701 ctgcacggca tcctctatgt gctggagtgc gacctgctgg acgacactgc caagcagctc
     8761 atcccggtca tcagcgacta tctcctctcc aacctgaaag ggatcgccca ctgcgtgaac
     8821 attcacagcc agcagcacgt actggtcatg tgtgccactg cgttttacct cattgagaac
     8881 tatcctctgg acgtagggcc ggaattttca gcatcaataa tacagatgtg tggggtgatg
     8941 ctgtctggaa gtgaggagtc caccccctcc atcatttacc actgtgccct cagaggcctg
     9001 gagcgcctcc tgctctctga gcagctctcc cgcctggatg cagaatcgct ggtcaagctg
     9061 agtgtggaca gagtgaacgt gcacagcccg caccgggcca tggcggctct gggcctgatg
     9121 ctcacctgca tgtacacagg aaaggagaaa gtcagtccgg gtagaacttc agaccctaat
     9181 cctgcagccc ccgacagcga gtcagtgatt gttgctatgg agcgggtatc tgttcttttt
     9241 gataggatca ggaaaggctt tccttgtgaa gccagagtgg tggccaggat cctgccccag
     9301 tttctagacg acttcttccc accccaggac atcatgaaca aagtcatcgg agagtttctg
     9361 tccaaccagc agccataccc ccagttcatg gccaccgtgg tgtataaggt gtttcagact
     9421 ctgcacagca ccgggcagtc gtccatggtc cgggactggg tcatgctgtc cctctccaac
     9481 ttcacgcaga gggccccggt cgccatggcc acgtggagcc tctcctgctt ctttgtcagc
     9541 gcgtccacca gcccgtgggt cgcggcgatc ctcccacatg tcatcagcag gatgggcaag
     9601 ctggagcagg tggacgtgaa ccttttctgc ctggtcgcca cagacttcta cagacaccag
     9661 atagaggagg agctcgaccg cagggccttc cagtctgtgc ttgaggtggt tgcagcccca
     9721 ggaagcccat atcaccggct gctgacttgt ttacgaaatg tccacaaggt caccacctgc
//
