LOCUS       sequence-209890-        9789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9107..9116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      241 caacagcaac aacagcagca acagcaacaa caacagcagc aacagcaaca acagcagcaa
      301 cagcaacaac agcagcaaca gcaacaacag cagcaacagc aacaacagca gcaacagcaa
      361 caacagcagc aacagcaaca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      421 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaaccg
      481 ccaccgccgc cgccgccgcc gccgcctcct cagcttcctc agccgccgcc gcaggcacag
      541 ccgctgctgc ctcagccgca gccgcccccg ccgccgcccc cgccgccacc cggcccggct
      601 gtggctgagg agccgctgca ccgaccaaag aaagaacttt cagctaccaa gaaagaccgt
      661 gtgaatcatt gtctgacaat atgtgaaaac atagtggcac agtctgtcag aaattctcca
      721 gaatttcaga aacttctggg catcgctatg gaactttttc tgctgtgcag tgatgacgca
      781 gagtcagatg tcaggatggt ggctgacgaa tgcctcaaca aagttatcaa agctttgatg
      841 gattctaatc ttccaaggtt acagctcgag ctctataagg aaattaaaaa gaatggtgcc
      901 cctcggagtt tgcgtgctgc cctgtggagg tttgctgagc tggctcacct ggttcggcct
      961 cagaaatgca ggccttacct ggtgaacctt ctgccgtgcc tgactcgaac aagcaagaga
     1021 cccgaagaat cagtccagga gaccttggct gcagctgttc ccaaaattat ggcttctttt
     1081 ggcaattttg caaatgacaa tgaaattaag gttttgttaa aggccttcat agcgaacctg
     1141 aagtcaagct cccccaccat tcggcggaca gcggctggat cagcagtgag catctgccag
     1201 cactcaagaa ggacacaata tttctatagt tggctactaa atgtgctctt aggcttactc
     1261 gttcctgtcg aggatgaaca ctccactctg ctgattcttg gcgtgctgct caccctgagg
     1321 tatttggtgc ccttgctgca gcagcaggtc aaggacacaa gcctgaaagg cagcttcgga
     1381 gtgacaagga aagaaatgga agtctctcct tctgcagagc agcttgtcca ggtttatgaa
     1441 ctgacgttac atcatacaca gcaccaagac cacaatgttg tgaccggagc cctggagctg
     1501 ttgcagcagc tcttcagaac gcctccaccc gagcttctgc aaaccctgac cgcagtcggg
     1561 ggcattgggc agctcaccgc tgctaaggag gagtctggtg gccgaagccg tagtgggagt
     1621 attgtggaac ttatagctgg agggggttcc tcatgcagcc ctgtcctttc aagaaaacaa
     1681 aaaggcaaag tgctcttagg agaagaagaa gccttggagg atgactctga atcgagatcg
     1741 gatgtcagca gctctgcctt aacagcctca gtgaaggatg agatcagtgg agagctggct
     1801 gcttcttcag gggtttccac tccagggtca gcaggtcatg acatcatcac agaacagcca
     1861 cggtcacagc acacactgca ggcggactca gtggatctgg ccagctgtga cttgacaagc
     1921 tctgccactg atggggatga ggaggatatc ttgagccaca gctccagcca ggtcagcgcc
     1981 gtcccatctg accctgccat ggacctgaat gatgggaccc aggcctcgtc gcccatcagc
     2041 gacagctccc agaccaccac cgaagggcct gattcagctg ttaccccttc agacagttct
     2101 gaaattgtgt tagacggtac cgacaaccag tatttgggcc tgcagattgg acagccccag
     2161 gatgaagatg aggaagccac aggtattctt cctgatgaag cctcggaggc cttcaggaac
     2221 tcttccatgg cccttcaaca ggcacattta ttgaaaaaca tgagtcactg caggcagcct
     2281 tctgacagca gtgttgataa atttgtgttg agagatgaag ctactgaacc gggtgatcaa
     2341 gaaaacaagc cttgccgcat caaaggtgac attggacagt ccactgatga tgactctgca
     2401 cctcttgtcc attgtgtccg ccttttatct gcttcgtttt tgctaacagg gggaaaaaat
     2461 gtgctggttc cggacaggga tgtgagggtc agcgtgaagg ccctggccct cagctgtgtg
     2521 ggagcagctg tggccctcca cccggaatct ttcttcagca aactctataa agttcctctt
     2581 gacaccacgg aataccctga ggaacagtat gtctcagaca tcttgaacta catcgatcat
     2641 ggagacccac aggttcgagg agccactgcc attctctgtg ggaccctcat ctgctccatc
     2701 ctcagcaggt cccgcttcca cgtgggagat tggatgggca ccattagaac cctcacagga
     2761 aatacatttt ctttggcgga ttgcattcct ttgctgcgga aaacactgaa ggatgagtct
     2821 tctgttactt gcaagttagc ttgtacagct gtgaggaact gtgtcatgag tctctgcagc
     2881 agcagctaca gtgagttagg actgcagctg atcatcgatg tgctgactct gaggaacagt
     2941 tcctattggc tggtgaggac agagcttctg gaaacccttg cagagattga cttcaggctg
     3001 gtgagctttt tggaggcaaa agcagaaaac ttacacagag gggctcatca ttatacaggg
     3061 cttttaaaac tgcaagaacg agtgctcaat aatgttgtca tccatttgct tggagatgaa
     3121 gaccccaggg tgcgacatgt tgccgcagca tcactaatta ggcttgtccc aaagctgttt
     3181 tataaatgtg accaaggaca agctgatcca gtagtggccg tggcaagaga tcaaagcagt
     3241 gtttacctga aacttctcat gcatgagacg cagcctccat ctcatttctc cgtcagcaca
     3301 ataaccagaa tatatagagg ctataaccta ctaccaagca taacagacgt cactatggaa
     3361 aataaccttt caagagttat tgcagcagtt tctcatgaac taatcacatc aaccaccaga
     3421 gcactcacat ttggatgctg tgaagctttg tgtcttcttt ccactgcctt cccagtttgc
     3481 atttggagtt taggttggca ctgtggagtg cctccactga gtgcctcaga tgagtctagg
     3541 aagagctgta ccgttgggat ggccacaatg attctgaccc tgctctcgtc agcttggttc
     3601 ccattggatc tctcagccca tcaagatgct ttgattttgg ccggaaactt gcttgcagcc
     3661 agtgctccca aatctctgag aagttcatgg gcctctgaag aagaagccaa cccagcagcc
     3721 accaagcaag aggaggtctg gccagccctg ggggaccggg ccctggtgcc catggtggag
     3781 cagctcttct ctcacctgct gaaggtgatt aacatttgtg cccacgtcct ggatgacgtg
     3841 gctcctggac ccgcaataaa ggcagccttg ccttctctaa caaacccccc ttctctaagt
     3901 cccatccgac gaaaggggaa ggagaaagaa ccaggagaac aagcatctgt accgttgagt
     3961 cccaagaaag gcagtgaggc cagtgcagct tctagacaat ctgatacctc aggtcctgtt
     4021 acaacaagta aatcctcatc actggggagt ttctatcatc ttccttcata cctcaaactg
     4081 catgatgtcc tgaaagctac acacgctaac tacaaggtca cgctggatct tcagaacagc
     4141 acggaaaagt ttggagggtt tcttcgctca gccttggatg ttctttctca gatactagag
     4201 ctggccacac tgcaggacat tgggaagtgt gttgaagaga tcctaggata cctgaaatcc
     4261 tgctttagtc gagaaccaat gatggcaact gtttgtgttc aacaattgtt gaagactctc
     4321 tttggcacaa acttggcctc ccagtttgat ggcttatctt ccaaccccag caagtcacaa
     4381 ggccgagcac agcgccttgg ctcctccagt gtgaggccag gcttgtacca ctactgcttc
     4441 atggccccgt acacccactt cacccaggcc ctcgctgacg ccagcctgag gaacatggtg
     4501 caggcggagc aggagaacga cacctcggga tggtttgatg tcctccagaa agtgtctacc
     4561 cagttgaaga caaacctcac gagtgtcaca aagaaccgtg cagataagaa tgctattcat
     4621 aatcacattc gtttgtttga acctcttgtt ataaaagctt taaaacagta cacgactaca
     4681 acatgtgtgc agttacagaa gcaggtttta gatttgctgg cgcagctggt tcagttacgg
     4741 gttaattact gtcttctgga ttcagatcag gtgtttattg gctttgtatt gaaacagttt
     4801 gaatacattg aagtgggcca gttcagggaa tcagaggcaa tcattccaaa catctttttc
     4861 ttcttggtat tactatctta tgaacgctat cattcaaaac agatcattgg aattcctaaa
     4921 atcattcagc tctgtgatgg catcatggcc agtggaagga aggctgtgac acatgccata
     4981 ccggctctgc agcccatagt ccacgacctc tttgtattaa gaggaacaaa taaagctgat
     5041 gcaggaaaag agcttgaaac ccaaaaagag gtggtggtgt caatgttact gagactcatc
     5101 cagtaccatc aggtgttgga gatgttcatt cttgtcctgc agcagtgcca caaggagaat
     5161 gaagacaagt ggaagcgact gtctcgacag atagctgaca tcatcctccc aatgttagcc
     5221 aaacagcaga tgcacattga ctctcatgaa gcccttggag tgttaaatac attatttgag
     5281 attttggccc cttcctccct ccgtccggta gacatgcttt tacggagtat gttcgtcact
     5341 ccaaacacaa tggcgtccgt gagcactgtt caactgtgga tatcgggaat tctggccatt
     5401 ttgagggttc tgatttccca gtcaactgaa gatattgttc tttctcgtat tcaggagctc
     5461 tccttctctc cgtatttaat ctcctgtaca gtaattaata ggttaagaga tggggacagt
     5521 acttcaacgc tagaagaaca cagtgaaggg aaacaaataa agaatttgcc agaagaaaca
     5581 ttttcaaggt ttctattaca actggttggt attcttttag aagacattgt tacaaaacag
     5641 ctgaaggtgg aaatgagtga gcagcaacat actttctatt gccaggaact aggcacactg
     5701 ctaatgtgtc tgatccacat cttcaagtct ggaatgttcc ggagaatcac agcagctgcc
     5761 actaggctgt tccgcagtga tggctgtggc ggcagtttct acaccctgga cagcttgaac
     5821 ttgcgggctc gttccatgat caccacccac ccggccctgg tgctgctctg gtgtcagata
     5881 ctgctgcttg tcaaccacac cgactaccgc tggtgggcag aagtgcagca gaccccgaaa
     5941 agacacagtc tgtccagcac aaagttactt agtccccaga tgtctggaga agaggaggat
     6001 tctgacttgg cagccaaact tggaatgtgc aatagagaaa tagtacgaag aggggctctc
     6061 attctcttct gtgattatgt ctgtcagaac ctccatgact ccgagcactt aacgtggctc
     6121 attgtaaatc acattcaaga tctgatcagc ctttcccacg agcctccagt acaggacttc
     6181 atcagtgccg ttcatcggaa ctctgctgcc agcggcctgt tcatccaggc aattcagtct
     6241 cgttgtgaaa acctttcaac tccaaccatg ctgaagaaaa ctcttcagtg cttggagggg
     6301 atccatctca gccagtcggg agctgtgctc acgctgtatg tggacaggct tctgtgcacc
     6361 cctttccgtg tgctggctcg catggtcgac atccttgctt gtcgccgggt agaaatgctt
     6421 ctggctgcaa atttacagag cagcatggcc cagttgccaa tggaagaact caacagaatc
     6481 caggaatacc ttcagagcag cgggctcgct cagagacacc aaaggctcta ttccctgctg
     6541 gacaggtttc gtctctccac catgcaagac tcacttagtc cctctcctcc agtctcttcc
     6601 cacccgctgg acggggatgg gcacgtgtca ctggaaacag tgagtccgga caaagactgg
     6661 tacgttcatc ttgtcaaatc ccagtgttgg accaggtcag attctgcact gctggaaggt
     6721 gcagagctgg tgaatcggat tcctgctgaa gatatgaatg ccttcatgat gaactcggag
     6781 ttcaacctaa gcctgctagc tccatgctta agcctaggga tgagtgaaat ttctggtggc
     6841 cagaagagtg ccctttttga agcagcccgt gaggtgactc tggcccgtgt gagcggcacc
     6901 gtgcagcagc tccctgctgt ccatcatgtc ttccagcccg agctgcctgc agaaccggcg
     6961 gcctactgga gcaagttgaa tgatctgttt ggggatgctg cactgtatca gtccctgccc
     7021 actctggccc gggccctggc acagtacctg gtggtggtct ccaaactgcc cagtcatttg
     7081 caccttcctc ctgagaaaga gaaggacatt gtgaaattcg tggtggcaac ccttgaggcc
     7141 ctgtcctggc atttgatcca tgagcagatc ccgctgagtc tggatctcca ggcagggctg
     7201 gactgctgct gcctggccct gcagctgcct ggcctctgga gcgtggtctc ctccacagag
     7261 tttgtgaccc acgcctgctc cctcatccac tgtgtgcact tcatcctgga ggccgttgca
     7321 gtgcagcctg gagagcagct tcttagtcca gaaagaagga caaatacccc aaaagccatc
     7381 agcgaggagg aggaggaagt agatccaaac acacagaatc ctaagtatat cactgcagcc
     7441 tgtgagatgg tggcagaaat ggtggagtct ctgcagtcgg tgttggcctt gggtcataaa
     7501 aggaatagcg gcgtgccggc gtttctcacg ccattgctca ggaacatcat catcagcctg
     7561 gcccgcctgc cccttgtcaa cagctacaca cgtgtgcccc cactggtgtg gaagcttgga
     7621 tggtcaccca aaccgggagg ggattttggc acagcattcc ctgagatccc cgtggagttc
     7681 ctccaggaaa aggaagtctt taaggagttc atctaccgca tcaacacact aggctggacc
     7741 agtcgtactc agtttgaaga aacttgggcc accctccttg gtgtcctggt gacgcagccc
     7801 ctcgtgatgg agcaggagga gagcccacca gaagaagaca cagagaggac ccagatcaac
     7861 gtcctggccg tgcaggccat cacctcactg gtgctcagtg caatgactgt gcctgtggcc
     7921 ggcaacccag ctgtaagctg cttggagcag cagccccgga acaagcctct gaaagctctc
     7981 gacaccaggt ttgggaggaa gctgagcatt atcagaggga ttgtggagca agagattcaa
     8041 gcaatggttt caaagagaga gaatattgcc acccatcatt tatatcaggc atgggatcct
     8101 gtcccttctc tgtctccggc tactacaggt gccctcatca gccacgagaa gctgctgctg
     8161 cagatcaacc ccgagcggga gctggggagc atgagctaca aactcggcca ggtgtccata
     8221 cactccgtgt ggctagggaa cagcatcaca cccctgaggg aggaggaatg ggacgaggaa
     8281 gaggaggagg aggccgacgc ccctgcacct tcgtcaccac ccacgtctcc agtcaactcc
     8341 aggaaacacc gggctggagt tgacatccac tcctgttcgc agtttttgct tgagttgtac
     8401 agccgctgga tcctgccgtc cagctcagcc aggaggaccc cggccatcct gatcagtgag
     8461 gtggtcagat cccttctagt ggtctcagac ttgttcaccg agcgcaacca gtttgagctg
     8521 atgtatgtga cgctgacaga actgcgaagg gtgcaccctt cagaagacga gatcctcgct
     8581 cagtacctgg tgcctgccac ctgcaaggca gctgccgtcc ttgggatgga caaggccgtg
     8641 gcggagcctg tcagccgcct gctggagagc acgctcagga gcagccacct gcccagcagg
     8701 gttggagccc tgcacggcat cctctatgtg ctggagtgcg acctgctgga cgacactgcc
     8761 aagcagctca tcccggtcat cagcgactat ctcctctcca acctgaaagg gatcgcccac
     8821 tgcgtgaaca ttcacagcca gcagcacgta ctggtcatgt gtgccactgc gttttacctc
     8881 attgagaact atcctctgga cgtagggccg gaattttcag catcaataat acagatgtgt
     8941 ggggtgatgc tgtctggaag tgaggagtcc accccctcca tcatttacca ctgtgccctc
     9001 agaggcctgg agcgcctcct gctctctgag cagctctccc gcctggatgc agaatcgctg
     9061 gtcaagctga gtgtggacag agtgaacgtg cacagcccgc accgggccat ggcggctctg
     9121 ggcctgatgc tcacctgcat gtacacagga aaggagaaag tcagtccggg tagaacttca
     9181 gaccctaatc ctgcagcccc cgacagcgag tcagtgattg ttgctatgga gcgggtatct
     9241 gttctttttg ataggatcag gaaaggcttt ccttgtgaag ccagagtggt ggccaggatc
     9301 ctgccccagt ttctagacga cttcttccca ccccaggaca tcatgaacaa agtcatcgga
     9361 gagtttctgt ccaaccagca gccatacccc cagttcatgg ccaccgtggt gtataaggtg
     9421 tttcagactc tgcacagcac cgggcagtcg tccatggtcc gggactgggt catgctgtcc
     9481 ctctccaact tcacgcagag ggccccggtc gccatggcca cgtggagcct ctcctgcttc
     9541 tttgtcagcg cgtccaccag cccgtgggtc gcggcgatcc tcccacatgt catcagcagg
     9601 atgggcaagc tggagcaggt ggacgtgaac cttttctgcc tggtcgccac agacttctac
     9661 agacaccaga tagaggagga gctcgaccgc agggccttcc agtctgtgct tgaggtggtt
     9721 gcagccccag gaagcccata tcaccggctg ctgacttgtt tacgaaatgt ccacaaggtc
     9781 accacctgc
//
