LOCUS       sequence-209892-        9420 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8738..8747
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaacc gccaccgccg
      121 ccgccgccgc cgccgcctcc tcagcttcct cagccgccgc cgcaggcaca gccgctgctg
      181 cctcagccgc agccgccccc gccgccgccc ccgccgccac ccggcccggc tgtggctgag
      241 gagccgctgc accgaccaaa gaaagaactt tcagctacca agaaagaccg tgtgaatcat
      301 tgtctgacaa tatgtgaaaa catagtggca cagtctgtca gaaattctcc agaatttcag
      361 aaacttctgg gcatcgctat ggaacttttt ctgctgtgca gtgatgacgc agagtcagat
      421 gtcaggatgg tggctgacga atgcctcaac aaagttatca aagctttgat ggattctaat
      481 cttccaaggt tacagctcga gctctataag gaaattaaaa agaatggtgc ccctcggagt
      541 ttgcgtgctg ccctgtggag gtttgctgag ctggctcacc tggttcggcc tcagaaatgc
      601 aggccttacc tggtgaacct tctgccgtgc ctgactcgaa caagcaagag acccgaagaa
      661 tcagtccagg agaccttggc tgcagctgtt cccaaaatta tggcttcttt tggcaatttt
      721 gcaaatgaca atgaaattaa ggttttgtta aaggccttca tagcgaacct gaagtcaagc
      781 tcccccacca ttcggcggac agcggctgga tcagcagtga gcatctgcca gcactcaaga
      841 aggacacaat atttctatag ttggctacta aatgtgctct taggcttact cgttcctgtc
      901 gaggatgaac actccactct gctgattctt ggcgtgctgc tcaccctgag gtatttggtg
      961 cccttgctgc agcagcaggt caaggacaca agcctgaaag gcagcttcgg agtgacaagg
     1021 aaagaaatgg aagtctctcc ttctgcagag cagcttgtcc aggtttatga actgacgtta
     1081 catcatacac agcaccaaga ccacaatgtt gtgaccggag ccctggagct gttgcagcag
     1141 ctcttcagaa cgcctccacc cgagcttctg caaaccctga ccgcagtcgg gggcattggg
     1201 cagctcaccg ctgctaagga ggagtctggt ggccgaagcc gtagtgggag tattgtggaa
     1261 cttatagctg gagggggttc ctcatgcagc cctgtccttt caagaaaaca aaaaggcaaa
     1321 gtgctcttag gagaagaaga agccttggag gatgactctg aatcgagatc ggatgtcagc
     1381 agctctgcct taacagcctc agtgaaggat gagatcagtg gagagctggc tgcttcttca
     1441 ggggtttcca ctccagggtc agcaggtcat gacatcatca cagaacagcc acggtcacag
     1501 cacacactgc aggcggactc agtggatctg gccagctgtg acttgacaag ctctgccact
     1561 gatggggatg aggaggatat cttgagccac agctccagcc aggtcagcgc cgtcccatct
     1621 gaccctgcca tggacctgaa tgatgggacc caggcctcgt cgcccatcag cgacagctcc
     1681 cagaccacca ccgaagggcc tgattcagct gttacccctt cagacagttc tgaaattgtg
     1741 ttagacggta ccgacaacca gtatttgggc ctgcagattg gacagcccca ggatgaagat
     1801 gaggaagcca caggtattct tcctgatgaa gcctcggagg ccttcaggaa ctcttccatg
     1861 gcccttcaac aggcacattt attgaaaaac atgagtcact gcaggcagcc ttctgacagc
     1921 agtgttgata aatttgtgtt gagagatgaa gctactgaac cgggtgatca agaaaacaag
     1981 ccttgccgca tcaaaggtga cattggacag tccactgatg atgactctgc acctcttgtc
     2041 cattgtgtcc gccttttatc tgcttcgttt ttgctaacag ggggaaaaaa tgtgctggtt
     2101 ccggacaggg atgtgagggt cagcgtgaag gccctggccc tcagctgtgt gggagcagct
     2161 gtggccctcc acccggaatc tttcttcagc aaactctata aagttcctct tgacaccacg
     2221 gaataccctg aggaacagta tgtctcagac atcttgaact acatcgatca tggagaccca
     2281 caggttcgag gagccactgc cattctctgt gggaccctca tctgctccat cctcagcagg
     2341 tcccgcttcc acgtgggaga ttggatgggc accattagaa ccctcacagg aaatacattt
     2401 tctttggcgg attgcattcc tttgctgcgg aaaacactga aggatgagtc ttctgttact
     2461 tgcaagttag cttgtacagc tgtgaggaac tgtgtcatga gtctctgcag cagcagctac
     2521 agtgagttag gactgcagct gatcatcgat gtgctgactc tgaggaacag ttcctattgg
     2581 ctggtgagga cagagcttct ggaaaccctt gcagagattg acttcaggct ggtgagcttt
     2641 ttggaggcaa aagcagaaaa cttacacaga ggggctcatc attatacagg gcttttaaaa
     2701 ctgcaagaac gagtgctcaa taatgttgtc atccatttgc ttggagatga agaccccagg
     2761 gtgcgacatg ttgccgcagc atcactaatt aggcttgtcc caaagctgtt ttataaatgt
     2821 gaccaaggac aagctgatcc agtagtggcc gtggcaagag atcaaagcag tgtttacctg
     2881 aaacttctca tgcatgagac gcagcctcca tctcatttct ccgtcagcac aataaccaga
     2941 atatatagag gctataacct actaccaagc ataacagacg tcactatgga aaataacctt
     3001 tcaagagtta ttgcagcagt ttctcatgaa ctaatcacat caaccaccag agcactcaca
     3061 tttggatgct gtgaagcttt gtgtcttctt tccactgcct tcccagtttg catttggagt
     3121 ttaggttggc actgtggagt gcctccactg agtgcctcag atgagtctag gaagagctgt
     3181 accgttggga tggccacaat gattctgacc ctgctctcgt cagcttggtt cccattggat
     3241 ctctcagccc atcaagatgc tttgattttg gccggaaact tgcttgcagc cagtgctccc
     3301 aaatctctga gaagttcatg ggcctctgaa gaagaagcca acccagcagc caccaagcaa
     3361 gaggaggtct ggccagccct gggggaccgg gccctggtgc ccatggtgga gcagctcttc
     3421 tctcacctgc tgaaggtgat taacatttgt gcccacgtcc tggatgacgt ggctcctgga
     3481 cccgcaataa aggcagcctt gccttctcta acaaaccccc cttctctaag tcccatccga
     3541 cgaaagggga aggagaaaga accaggagaa caagcatctg taccgttgag tcccaagaaa
     3601 ggcagtgagg ccagtgcagc ttctagacaa tctgatacct caggtcctgt tacaacaagt
     3661 aaatcctcat cactggggag tttctatcat cttccttcat acctcaaact gcatgatgtc
     3721 ctgaaagcta cacacgctaa ctacaaggtc acgctggatc ttcagaacag cacggaaaag
     3781 tttggagggt ttcttcgctc agccttggat gttctttctc agatactaga gctggccaca
     3841 ctgcaggaca ttgggaagtg tgttgaagag atcctaggat acctgaaatc ctgctttagt
     3901 cgagaaccaa tgatggcaac tgtttgtgtt caacaattgt tgaagactct ctttggcaca
     3961 aacttggcct cccagtttga tggcttatct tccaacccca gcaagtcaca aggccgagca
     4021 cagcgccttg gctcctccag tgtgaggcca ggcttgtacc actactgctt catggccccg
     4081 tacacccact tcacccaggc cctcgctgac gccagcctga ggaacatggt gcaggcggag
     4141 caggagaacg acacctcggg atggtttgat gtcctccaga aagtgtctac ccagttgaag
     4201 acaaacctca cgagtgtcac aaagaaccgt gcagataaga atgctattca taatcacatt
     4261 cgtttgtttg aacctcttgt tataaaagct ttaaaacagt acacgactac aacatgtgtg
     4321 cagttacaga agcaggtttt agatttgctg gcgcagctgg ttcagttacg ggttaattac
     4381 tgtcttctgg attcagatca ggtgtttatt ggctttgtat tgaaacagtt tgaatacatt
     4441 gaagtgggcc agttcaggga atcagaggca atcattccaa acatcttttt cttcttggta
     4501 ttactatctt atgaacgcta tcattcaaaa cagatcattg gaattcctaa aatcattcag
     4561 ctctgtgatg gcatcatggc cagtggaagg aaggctgtga cacatgccat accggctctg
     4621 cagcccatag tccacgacct ctttgtatta agaggaacaa ataaagctga tgcaggaaaa
     4681 gagcttgaaa cccaaaaaga ggtggtggtg tcaatgttac tgagactcat ccagtaccat
     4741 caggtgttgg agatgttcat tcttgtcctg cagcagtgcc acaaggagaa tgaagacaag
     4801 tggaagcgac tgtctcgaca gatagctgac atcatcctcc caatgttagc caaacagcag
     4861 atgcacattg actctcatga agcccttgga gtgttaaata cattatttga gattttggcc
     4921 ccttcctccc tccgtccggt agacatgctt ttacggagta tgttcgtcac tccaaacaca
     4981 atggcgtccg tgagcactgt tcaactgtgg atatcgggaa ttctggccat tttgagggtt
     5041 ctgatttccc agtcaactga agatattgtt ctttctcgta ttcaggagct ctccttctct
     5101 ccgtatttaa tctcctgtac agtaattaat aggttaagag atggggacag tacttcaacg
     5161 ctagaagaac acagtgaagg gaaacaaata aagaatttgc cagaagaaac attttcaagg
     5221 tttctattac aactggttgg tattctttta gaagacattg ttacaaaaca gctgaaggtg
     5281 gaaatgagtg agcagcaaca tactttctat tgccaggaac taggcacact gctaatgtgt
     5341 ctgatccaca tcttcaagtc tggaatgttc cggagaatca cagcagctgc cactaggctg
     5401 ttccgcagtg atggctgtgg cggcagtttc tacaccctgg acagcttgaa cttgcgggct
     5461 cgttccatga tcaccaccca cccggccctg gtgctgctct ggtgtcagat actgctgctt
     5521 gtcaaccaca ccgactaccg ctggtgggca gaagtgcagc agaccccgaa aagacacagt
     5581 ctgtccagca caaagttact tagtccccag atgtctggag aagaggagga ttctgacttg
     5641 gcagccaaac ttggaatgtg caatagagaa atagtacgaa gaggggctct cattctcttc
     5701 tgtgattatg tctgtcagaa cctccatgac tccgagcact taacgtggct cattgtaaat
     5761 cacattcaag atctgatcag cctttcccac gagcctccag tacaggactt catcagtgcc
     5821 gttcatcgga actctgctgc cagcggcctg ttcatccagg caattcagtc tcgttgtgaa
     5881 aacctttcaa ctccaaccat gctgaagaaa actcttcagt gcttggaggg gatccatctc
     5941 agccagtcgg gagctgtgct cacgctgtat gtggacaggc ttctgtgcac ccctttccgt
     6001 gtgctggctc gcatggtcga catccttgct tgtcgccggg tagaaatgct tctggctgca
     6061 aatttacaga gcagcatggc ccagttgcca atggaagaac tcaacagaat ccaggaatac
     6121 cttcagagca gcgggctcgc tcagagacac caaaggctct attccctgct ggacaggttt
     6181 cgtctctcca ccatgcaaga ctcacttagt ccctctcctc cagtctcttc ccacccgctg
     6241 gacggggatg ggcacgtgtc actggaaaca gtgagtccgg acaaagactg gtacgttcat
     6301 cttgtcaaat cccagtgttg gaccaggtca gattctgcac tgctggaagg tgcagagctg
     6361 gtgaatcgga ttcctgctga agatatgaat gccttcatga tgaactcgga gttcaaccta
     6421 agcctgctag ctccatgctt aagcctaggg atgagtgaaa tttctggtgg ccagaagagt
     6481 gccctttttg aagcagcccg tgaggtgact ctggcccgtg tgagcggcac cgtgcagcag
     6541 ctccctgctg tccatcatgt cttccagccc gagctgcctg cagaaccggc ggcctactgg
     6601 agcaagttga atgatctgtt tggggatgct gcactgtatc agtccctgcc cactctggcc
     6661 cgggccctgg cacagtacct ggtggtggtc tccaaactgc ccagtcattt gcaccttcct
     6721 cctgagaaag agaaggacat tgtgaaattc gtggtggcaa cccttgaggc cctgtcctgg
     6781 catttgatcc atgagcagat cccgctgagt ctggatctcc aggcagggct ggactgctgc
     6841 tgcctggccc tgcagctgcc tggcctctgg agcgtggtct cctccacaga gtttgtgacc
     6901 cacgcctgct ccctcatcca ctgtgtgcac ttcatcctgg aggccgttgc agtgcagcct
     6961 ggagagcagc ttcttagtcc agaaagaagg acaaataccc caaaagccat cagcgaggag
     7021 gaggaggaag tagatccaaa cacacagaat cctaagtata tcactgcagc ctgtgagatg
     7081 gtggcagaaa tggtggagtc tctgcagtcg gtgttggcct tgggtcataa aaggaatagc
     7141 ggcgtgccgg cgtttctcac gccattgctc aggaacatca tcatcagcct ggcccgcctg
     7201 ccccttgtca acagctacac acgtgtgccc ccactggtgt ggaagcttgg atggtcaccc
     7261 aaaccgggag gggattttgg cacagcattc cctgagatcc ccgtggagtt cctccaggaa
     7321 aaggaagtct ttaaggagtt catctaccgc atcaacacac taggctggac cagtcgtact
     7381 cagtttgaag aaacttgggc caccctcctt ggtgtcctgg tgacgcagcc cctcgtgatg
     7441 gagcaggagg agagcccacc agaagaagac acagagagga cccagatcaa cgtcctggcc
     7501 gtgcaggcca tcacctcact ggtgctcagt gcaatgactg tgcctgtggc cggcaaccca
     7561 gctgtaagct gcttggagca gcagccccgg aacaagcctc tgaaagctct cgacaccagg
     7621 tttgggagga agctgagcat tatcagaggg attgtggagc aagagattca agcaatggtt
     7681 tcaaagagag agaatattgc cacccatcat ttatatcagg catgggatcc tgtcccttct
     7741 ctgtctccgg ctactacagg tgccctcatc agccacgaga agctgctgct gcagatcaac
     7801 cccgagcggg agctggggag catgagctac aaactcggcc aggtgtccat acactccgtg
     7861 tggctaggga acagcatcac acccctgagg gaggaggaat gggacgagga agaggaggag
     7921 gaggccgacg cccctgcacc ttcgtcacca cccacgtctc cagtcaactc caggaaacac
     7981 cgggctggag ttgacatcca ctcctgttcg cagtttttgc ttgagttgta cagccgctgg
     8041 atcctgccgt ccagctcagc caggaggacc ccggccatcc tgatcagtga ggtggtcaga
     8101 tcccttctag tggtctcaga cttgttcacc gagcgcaacc agtttgagct gatgtatgtg
     8161 acgctgacag aactgcgaag ggtgcaccct tcagaagacg agatcctcgc tcagtacctg
     8221 gtgcctgcca cctgcaaggc agctgccgtc cttgggatgg acaaggccgt ggcggagcct
     8281 gtcagccgcc tgctggagag cacgctcagg agcagccacc tgcccagcag ggttggagcc
     8341 ctgcacggca tcctctatgt gctggagtgc gacctgctgg acgacactgc caagcagctc
     8401 atcccggtca tcagcgacta tctcctctcc aacctgaaag ggatcgccca ctgcgtgaac
     8461 attcacagcc agcagcacgt actggtcatg tgtgccactg cgttttacct cattgagaac
     8521 tatcctctgg acgtagggcc ggaattttca gcatcaataa tacagatgtg tggggtgatg
     8581 ctgtctggaa gtgaggagtc caccccctcc atcatttacc actgtgccct cagaggcctg
     8641 gagcgcctcc tgctctctga gcagctctcc cgcctggatg cagaatcgct ggtcaagctg
     8701 agtgtggaca gagtgaacgt gcacagcccg caccgggcca tggcggctct gggcctgatg
     8761 ctcacctgca tgtacacagg aaaggagaaa gtcagtccgg gtagaacttc agaccctaat
     8821 cctgcagccc ccgacagcga gtcagtgatt gttgctatgg agcgggtatc tgttcttttt
     8881 gataggatca ggaaaggctt tccttgtgaa gccagagtgg tggccaggat cctgccccag
     8941 tttctagacg acttcttccc accccaggac atcatgaaca aagtcatcgg agagtttctg
     9001 tccaaccagc agccataccc ccagttcatg gccaccgtgg tgtataaggt gtttcagact
     9061 ctgcacagca ccgggcagtc gtccatggtc cgggactggg tcatgctgtc cctctccaac
     9121 ttcacgcaga gggccccggt cgccatggcc acgtggagcc tctcctgctt ctttgtcagc
     9181 gcgtccacca gcccgtgggt cgcggcgatc ctcccacatg tcatcagcag gatgggcaag
     9241 ctggagcagg tggacgtgaa ccttttctgc ctggtcgcca cagacttcta cagacaccag
     9301 atagaggagg agctcgaccg cagggccttc cagtctgtgc ttgaggtggt tgcagcccca
     9361 ggaagcccat atcaccggct gctgacttgt ttacgaaatg tccacaaggt caccacctgc
//
