LOCUS       sequence-209893-        9435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8753..8762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caaccgccac cgccgccgcc gccgccgccg cctcctcagc ttcctcagcc gccgccgcag
      181 gcacagccgc tgctgcctca gccgcagccg cccccgccgc cgcccccgcc gccacccggc
      241 ccggctgtgg ctgaggagcc gctgcaccga ccaaagaaag aactttcagc taccaagaaa
      301 gaccgtgtga atcattgtct gacaatatgt gaaaacatag tggcacagtc tgtcagaaat
      361 tctccagaat ttcagaaact tctgggcatc gctatggaac tttttctgct gtgcagtgat
      421 gacgcagagt cagatgtcag gatggtggct gacgaatgcc tcaacaaagt tatcaaagct
      481 ttgatggatt ctaatcttcc aaggttacag ctcgagctct ataaggaaat taaaaagaat
      541 ggtgcccctc ggagtttgcg tgctgccctg tggaggtttg ctgagctggc tcacctggtt
      601 cggcctcaga aatgcaggcc ttacctggtg aaccttctgc cgtgcctgac tcgaacaagc
      661 aagagacccg aagaatcagt ccaggagacc ttggctgcag ctgttcccaa aattatggct
      721 tcttttggca attttgcaaa tgacaatgaa attaaggttt tgttaaaggc cttcatagcg
      781 aacctgaagt caagctcccc caccattcgg cggacagcgg ctggatcagc agtgagcatc
      841 tgccagcact caagaaggac acaatatttc tatagttggc tactaaatgt gctcttaggc
      901 ttactcgttc ctgtcgagga tgaacactcc actctgctga ttcttggcgt gctgctcacc
      961 ctgaggtatt tggtgccctt gctgcagcag caggtcaagg acacaagcct gaaaggcagc
     1021 ttcggagtga caaggaaaga aatggaagtc tctccttctg cagagcagct tgtccaggtt
     1081 tatgaactga cgttacatca tacacagcac caagaccaca atgttgtgac cggagccctg
     1141 gagctgttgc agcagctctt cagaacgcct ccacccgagc ttctgcaaac cctgaccgca
     1201 gtcgggggca ttgggcagct caccgctgct aaggaggagt ctggtggccg aagccgtagt
     1261 gggagtattg tggaacttat agctggaggg ggttcctcat gcagccctgt cctttcaaga
     1321 aaacaaaaag gcaaagtgct cttaggagaa gaagaagcct tggaggatga ctctgaatcg
     1381 agatcggatg tcagcagctc tgccttaaca gcctcagtga aggatgagat cagtggagag
     1441 ctggctgctt cttcaggggt ttccactcca gggtcagcag gtcatgacat catcacagaa
     1501 cagccacggt cacagcacac actgcaggcg gactcagtgg atctggccag ctgtgacttg
     1561 acaagctctg ccactgatgg ggatgaggag gatatcttga gccacagctc cagccaggtc
     1621 agcgccgtcc catctgaccc tgccatggac ctgaatgatg ggacccaggc ctcgtcgccc
     1681 atcagcgaca gctcccagac caccaccgaa gggcctgatt cagctgttac cccttcagac
     1741 agttctgaaa ttgtgttaga cggtaccgac aaccagtatt tgggcctgca gattggacag
     1801 ccccaggatg aagatgagga agccacaggt attcttcctg atgaagcctc ggaggccttc
     1861 aggaactctt ccatggccct tcaacaggca catttattga aaaacatgag tcactgcagg
     1921 cagccttctg acagcagtgt tgataaattt gtgttgagag atgaagctac tgaaccgggt
     1981 gatcaagaaa acaagccttg ccgcatcaaa ggtgacattg gacagtccac tgatgatgac
     2041 tctgcacctc ttgtccattg tgtccgcctt ttatctgctt cgtttttgct aacaggggga
     2101 aaaaatgtgc tggttccgga cagggatgtg agggtcagcg tgaaggccct ggccctcagc
     2161 tgtgtgggag cagctgtggc cctccacccg gaatctttct tcagcaaact ctataaagtt
     2221 cctcttgaca ccacggaata ccctgaggaa cagtatgtct cagacatctt gaactacatc
     2281 gatcatggag acccacaggt tcgaggagcc actgccattc tctgtgggac cctcatctgc
     2341 tccatcctca gcaggtcccg cttccacgtg ggagattgga tgggcaccat tagaaccctc
     2401 acaggaaata cattttcttt ggcggattgc attcctttgc tgcggaaaac actgaaggat
     2461 gagtcttctg ttacttgcaa gttagcttgt acagctgtga ggaactgtgt catgagtctc
     2521 tgcagcagca gctacagtga gttaggactg cagctgatca tcgatgtgct gactctgagg
     2581 aacagttcct attggctggt gaggacagag cttctggaaa cccttgcaga gattgacttc
     2641 aggctggtga gctttttgga ggcaaaagca gaaaacttac acagaggggc tcatcattat
     2701 acagggcttt taaaactgca agaacgagtg ctcaataatg ttgtcatcca tttgcttgga
     2761 gatgaagacc ccagggtgcg acatgttgcc gcagcatcac taattaggct tgtcccaaag
     2821 ctgttttata aatgtgacca aggacaagct gatccagtag tggccgtggc aagagatcaa
     2881 agcagtgttt acctgaaact tctcatgcat gagacgcagc ctccatctca tttctccgtc
     2941 agcacaataa ccagaatata tagaggctat aacctactac caagcataac agacgtcact
     3001 atggaaaata acctttcaag agttattgca gcagtttctc atgaactaat cacatcaacc
     3061 accagagcac tcacatttgg atgctgtgaa gctttgtgtc ttctttccac tgccttccca
     3121 gtttgcattt ggagtttagg ttggcactgt ggagtgcctc cactgagtgc ctcagatgag
     3181 tctaggaaga gctgtaccgt tgggatggcc acaatgattc tgaccctgct ctcgtcagct
     3241 tggttcccat tggatctctc agcccatcaa gatgctttga ttttggccgg aaacttgctt
     3301 gcagccagtg ctcccaaatc tctgagaagt tcatgggcct ctgaagaaga agccaaccca
     3361 gcagccacca agcaagagga ggtctggcca gccctggggg accgggccct ggtgcccatg
     3421 gtggagcagc tcttctctca cctgctgaag gtgattaaca tttgtgccca cgtcctggat
     3481 gacgtggctc ctggacccgc aataaaggca gccttgcctt ctctaacaaa ccccccttct
     3541 ctaagtccca tccgacgaaa ggggaaggag aaagaaccag gagaacaagc atctgtaccg
     3601 ttgagtccca agaaaggcag tgaggccagt gcagcttcta gacaatctga tacctcaggt
     3661 cctgttacaa caagtaaatc ctcatcactg gggagtttct atcatcttcc ttcatacctc
     3721 aaactgcatg atgtcctgaa agctacacac gctaactaca aggtcacgct ggatcttcag
     3781 aacagcacgg aaaagtttgg agggtttctt cgctcagcct tggatgttct ttctcagata
     3841 ctagagctgg ccacactgca ggacattggg aagtgtgttg aagagatcct aggatacctg
     3901 aaatcctgct ttagtcgaga accaatgatg gcaactgttt gtgttcaaca attgttgaag
     3961 actctctttg gcacaaactt ggcctcccag tttgatggct tatcttccaa ccccagcaag
     4021 tcacaaggcc gagcacagcg ccttggctcc tccagtgtga ggccaggctt gtaccactac
     4081 tgcttcatgg ccccgtacac ccacttcacc caggccctcg ctgacgccag cctgaggaac
     4141 atggtgcagg cggagcagga gaacgacacc tcgggatggt ttgatgtcct ccagaaagtg
     4201 tctacccagt tgaagacaaa cctcacgagt gtcacaaaga accgtgcaga taagaatgct
     4261 attcataatc acattcgttt gtttgaacct cttgttataa aagctttaaa acagtacacg
     4321 actacaacat gtgtgcagtt acagaagcag gttttagatt tgctggcgca gctggttcag
     4381 ttacgggtta attactgtct tctggattca gatcaggtgt ttattggctt tgtattgaaa
     4441 cagtttgaat acattgaagt gggccagttc agggaatcag aggcaatcat tccaaacatc
     4501 tttttcttct tggtattact atcttatgaa cgctatcatt caaaacagat cattggaatt
     4561 cctaaaatca ttcagctctg tgatggcatc atggccagtg gaaggaaggc tgtgacacat
     4621 gccataccgg ctctgcagcc catagtccac gacctctttg tattaagagg aacaaataaa
     4681 gctgatgcag gaaaagagct tgaaacccaa aaagaggtgg tggtgtcaat gttactgaga
     4741 ctcatccagt accatcaggt gttggagatg ttcattcttg tcctgcagca gtgccacaag
     4801 gagaatgaag acaagtggaa gcgactgtct cgacagatag ctgacatcat cctcccaatg
     4861 ttagccaaac agcagatgca cattgactct catgaagccc ttggagtgtt aaatacatta
     4921 tttgagattt tggccccttc ctccctccgt ccggtagaca tgcttttacg gagtatgttc
     4981 gtcactccaa acacaatggc gtccgtgagc actgttcaac tgtggatatc gggaattctg
     5041 gccattttga gggttctgat ttcccagtca actgaagata ttgttctttc tcgtattcag
     5101 gagctctcct tctctccgta tttaatctcc tgtacagtaa ttaataggtt aagagatggg
     5161 gacagtactt caacgctaga agaacacagt gaagggaaac aaataaagaa tttgccagaa
     5221 gaaacatttt caaggtttct attacaactg gttggtattc ttttagaaga cattgttaca
     5281 aaacagctga aggtggaaat gagtgagcag caacatactt tctattgcca ggaactaggc
     5341 acactgctaa tgtgtctgat ccacatcttc aagtctggaa tgttccggag aatcacagca
     5401 gctgccacta ggctgttccg cagtgatggc tgtggcggca gtttctacac cctggacagc
     5461 ttgaacttgc gggctcgttc catgatcacc acccacccgg ccctggtgct gctctggtgt
     5521 cagatactgc tgcttgtcaa ccacaccgac taccgctggt gggcagaagt gcagcagacc
     5581 ccgaaaagac acagtctgtc cagcacaaag ttacttagtc cccagatgtc tggagaagag
     5641 gaggattctg acttggcagc caaacttgga atgtgcaata gagaaatagt acgaagaggg
     5701 gctctcattc tcttctgtga ttatgtctgt cagaacctcc atgactccga gcacttaacg
     5761 tggctcattg taaatcacat tcaagatctg atcagccttt cccacgagcc tccagtacag
     5821 gacttcatca gtgccgttca tcggaactct gctgccagcg gcctgttcat ccaggcaatt
     5881 cagtctcgtt gtgaaaacct ttcaactcca accatgctga agaaaactct tcagtgcttg
     5941 gaggggatcc atctcagcca gtcgggagct gtgctcacgc tgtatgtgga caggcttctg
     6001 tgcacccctt tccgtgtgct ggctcgcatg gtcgacatcc ttgcttgtcg ccgggtagaa
     6061 atgcttctgg ctgcaaattt acagagcagc atggcccagt tgccaatgga agaactcaac
     6121 agaatccagg aataccttca gagcagcggg ctcgctcaga gacaccaaag gctctattcc
     6181 ctgctggaca ggtttcgtct ctccaccatg caagactcac ttagtccctc tcctccagtc
     6241 tcttcccacc cgctggacgg ggatgggcac gtgtcactgg aaacagtgag tccggacaaa
     6301 gactggtacg ttcatcttgt caaatcccag tgttggacca ggtcagattc tgcactgctg
     6361 gaaggtgcag agctggtgaa tcggattcct gctgaagata tgaatgcctt catgatgaac
     6421 tcggagttca acctaagcct gctagctcca tgcttaagcc tagggatgag tgaaatttct
     6481 ggtggccaga agagtgccct ttttgaagca gcccgtgagg tgactctggc ccgtgtgagc
     6541 ggcaccgtgc agcagctccc tgctgtccat catgtcttcc agcccgagct gcctgcagaa
     6601 ccggcggcct actggagcaa gttgaatgat ctgtttgggg atgctgcact gtatcagtcc
     6661 ctgcccactc tggcccgggc cctggcacag tacctggtgg tggtctccaa actgcccagt
     6721 catttgcacc ttcctcctga gaaagagaag gacattgtga aattcgtggt ggcaaccctt
     6781 gaggccctgt cctggcattt gatccatgag cagatcccgc tgagtctgga tctccaggca
     6841 gggctggact gctgctgcct ggccctgcag ctgcctggcc tctggagcgt ggtctcctcc
     6901 acagagtttg tgacccacgc ctgctccctc atccactgtg tgcacttcat cctggaggcc
     6961 gttgcagtgc agcctggaga gcagcttctt agtccagaaa gaaggacaaa taccccaaaa
     7021 gccatcagcg aggaggagga ggaagtagat ccaaacacac agaatcctaa gtatatcact
     7081 gcagcctgtg agatggtggc agaaatggtg gagtctctgc agtcggtgtt ggccttgggt
     7141 cataaaagga atagcggcgt gccggcgttt ctcacgccat tgctcaggaa catcatcatc
     7201 agcctggccc gcctgcccct tgtcaacagc tacacacgtg tgcccccact ggtgtggaag
     7261 cttggatggt cacccaaacc gggaggggat tttggcacag cattccctga gatccccgtg
     7321 gagttcctcc aggaaaagga agtctttaag gagttcatct accgcatcaa cacactaggc
     7381 tggaccagtc gtactcagtt tgaagaaact tgggccaccc tccttggtgt cctggtgacg
     7441 cagcccctcg tgatggagca ggaggagagc ccaccagaag aagacacaga gaggacccag
     7501 atcaacgtcc tggccgtgca ggccatcacc tcactggtgc tcagtgcaat gactgtgcct
     7561 gtggccggca acccagctgt aagctgcttg gagcagcagc cccggaacaa gcctctgaaa
     7621 gctctcgaca ccaggtttgg gaggaagctg agcattatca gagggattgt ggagcaagag
     7681 attcaagcaa tggtttcaaa gagagagaat attgccaccc atcatttata tcaggcatgg
     7741 gatcctgtcc cttctctgtc tccggctact acaggtgccc tcatcagcca cgagaagctg
     7801 ctgctgcaga tcaaccccga gcgggagctg gggagcatga gctacaaact cggccaggtg
     7861 tccatacact ccgtgtggct agggaacagc atcacacccc tgagggagga ggaatgggac
     7921 gaggaagagg aggaggaggc cgacgcccct gcaccttcgt caccacccac gtctccagtc
     7981 aactccagga aacaccgggc tggagttgac atccactcct gttcgcagtt tttgcttgag
     8041 ttgtacagcc gctggatcct gccgtccagc tcagccagga ggaccccggc catcctgatc
     8101 agtgaggtgg tcagatccct tctagtggtc tcagacttgt tcaccgagcg caaccagttt
     8161 gagctgatgt atgtgacgct gacagaactg cgaagggtgc acccttcaga agacgagatc
     8221 ctcgctcagt acctggtgcc tgccacctgc aaggcagctg ccgtccttgg gatggacaag
     8281 gccgtggcgg agcctgtcag ccgcctgctg gagagcacgc tcaggagcag ccacctgccc
     8341 agcagggttg gagccctgca cggcatcctc tatgtgctgg agtgcgacct gctggacgac
     8401 actgccaagc agctcatccc ggtcatcagc gactatctcc tctccaacct gaaagggatc
     8461 gcccactgcg tgaacattca cagccagcag cacgtactgg tcatgtgtgc cactgcgttt
     8521 tacctcattg agaactatcc tctggacgta gggccggaat tttcagcatc aataatacag
     8581 atgtgtgggg tgatgctgtc tggaagtgag gagtccaccc cctccatcat ttaccactgt
     8641 gccctcagag gcctggagcg cctcctgctc tctgagcagc tctcccgcct ggatgcagaa
     8701 tcgctggtca agctgagtgt ggacagagtg aacgtgcaca gcccgcaccg ggccatggcg
     8761 gctctgggcc tgatgctcac ctgcatgtac acaggaaagg agaaagtcag tccgggtaga
     8821 acttcagacc ctaatcctgc agcccccgac agcgagtcag tgattgttgc tatggagcgg
     8881 gtatctgttc tttttgatag gatcaggaaa ggctttcctt gtgaagccag agtggtggcc
     8941 aggatcctgc cccagtttct agacgacttc ttcccacccc aggacatcat gaacaaagtc
     9001 atcggagagt ttctgtccaa ccagcagcca tacccccagt tcatggccac cgtggtgtat
     9061 aaggtgtttc agactctgca cagcaccggg cagtcgtcca tggtccggga ctgggtcatg
     9121 ctgtccctct ccaacttcac gcagagggcc ccggtcgcca tggccacgtg gagcctctcc
     9181 tgcttctttg tcagcgcgtc caccagcccg tgggtcgcgg cgatcctccc acatgtcatc
     9241 agcaggatgg gcaagctgga gcaggtggac gtgaaccttt tctgcctggt cgccacagac
     9301 ttctacagac accagataga ggaggagctc gaccgcaggg ccttccagtc tgtgcttgag
     9361 gtggttgcag ccccaggaag cccatatcac cggctgctga cttgtttacg aaatgtccac
     9421 aaggtcacca cctgc
//
