LOCUS       sequence-209894-        9438 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8756..8765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaaccgc caccgccgcc gccgccgccg ccgcctcctc agcttcctca gccgccgccg
      181 caggcacagc cgctgctgcc tcagccgcag ccgcccccgc cgccgccccc gccgccaccc
      241 ggcccggctg tggctgagga gccgctgcac cgaccaaaga aagaactttc agctaccaag
      301 aaagaccgtg tgaatcattg tctgacaata tgtgaaaaca tagtggcaca gtctgtcaga
      361 aattctccag aatttcagaa acttctgggc atcgctatgg aactttttct gctgtgcagt
      421 gatgacgcag agtcagatgt caggatggtg gctgacgaat gcctcaacaa agttatcaaa
      481 gctttgatgg attctaatct tccaaggtta cagctcgagc tctataagga aattaaaaag
      541 aatggtgccc ctcggagttt gcgtgctgcc ctgtggaggt ttgctgagct ggctcacctg
      601 gttcggcctc agaaatgcag gccttacctg gtgaaccttc tgccgtgcct gactcgaaca
      661 agcaagagac ccgaagaatc agtccaggag accttggctg cagctgttcc caaaattatg
      721 gcttcttttg gcaattttgc aaatgacaat gaaattaagg ttttgttaaa ggccttcata
      781 gcgaacctga agtcaagctc ccccaccatt cggcggacag cggctggatc agcagtgagc
      841 atctgccagc actcaagaag gacacaatat ttctatagtt ggctactaaa tgtgctctta
      901 ggcttactcg ttcctgtcga ggatgaacac tccactctgc tgattcttgg cgtgctgctc
      961 accctgaggt atttggtgcc cttgctgcag cagcaggtca aggacacaag cctgaaaggc
     1021 agcttcggag tgacaaggaa agaaatggaa gtctctcctt ctgcagagca gcttgtccag
     1081 gtttatgaac tgacgttaca tcatacacag caccaagacc acaatgttgt gaccggagcc
     1141 ctggagctgt tgcagcagct cttcagaacg cctccacccg agcttctgca aaccctgacc
     1201 gcagtcgggg gcattgggca gctcaccgct gctaaggagg agtctggtgg ccgaagccgt
     1261 agtgggagta ttgtggaact tatagctgga gggggttcct catgcagccc tgtcctttca
     1321 agaaaacaaa aaggcaaagt gctcttagga gaagaagaag ccttggagga tgactctgaa
     1381 tcgagatcgg atgtcagcag ctctgcctta acagcctcag tgaaggatga gatcagtgga
     1441 gagctggctg cttcttcagg ggtttccact ccagggtcag caggtcatga catcatcaca
     1501 gaacagccac ggtcacagca cacactgcag gcggactcag tggatctggc cagctgtgac
     1561 ttgacaagct ctgccactga tggggatgag gaggatatct tgagccacag ctccagccag
     1621 gtcagcgccg tcccatctga ccctgccatg gacctgaatg atgggaccca ggcctcgtcg
     1681 cccatcagcg acagctccca gaccaccacc gaagggcctg attcagctgt taccccttca
     1741 gacagttctg aaattgtgtt agacggtacc gacaaccagt atttgggcct gcagattgga
     1801 cagccccagg atgaagatga ggaagccaca ggtattcttc ctgatgaagc ctcggaggcc
     1861 ttcaggaact cttccatggc ccttcaacag gcacatttat tgaaaaacat gagtcactgc
     1921 aggcagcctt ctgacagcag tgttgataaa tttgtgttga gagatgaagc tactgaaccg
     1981 ggtgatcaag aaaacaagcc ttgccgcatc aaaggtgaca ttggacagtc cactgatgat
     2041 gactctgcac ctcttgtcca ttgtgtccgc cttttatctg cttcgttttt gctaacaggg
     2101 ggaaaaaatg tgctggttcc ggacagggat gtgagggtca gcgtgaaggc cctggccctc
     2161 agctgtgtgg gagcagctgt ggccctccac ccggaatctt tcttcagcaa actctataaa
     2221 gttcctcttg acaccacgga ataccctgag gaacagtatg tctcagacat cttgaactac
     2281 atcgatcatg gagacccaca ggttcgagga gccactgcca ttctctgtgg gaccctcatc
     2341 tgctccatcc tcagcaggtc ccgcttccac gtgggagatt ggatgggcac cattagaacc
     2401 ctcacaggaa atacattttc tttggcggat tgcattcctt tgctgcggaa aacactgaag
     2461 gatgagtctt ctgttacttg caagttagct tgtacagctg tgaggaactg tgtcatgagt
     2521 ctctgcagca gcagctacag tgagttagga ctgcagctga tcatcgatgt gctgactctg
     2581 aggaacagtt cctattggct ggtgaggaca gagcttctgg aaacccttgc agagattgac
     2641 ttcaggctgg tgagcttttt ggaggcaaaa gcagaaaact tacacagagg ggctcatcat
     2701 tatacagggc ttttaaaact gcaagaacga gtgctcaata atgttgtcat ccatttgctt
     2761 ggagatgaag accccagggt gcgacatgtt gccgcagcat cactaattag gcttgtccca
     2821 aagctgtttt ataaatgtga ccaaggacaa gctgatccag tagtggccgt ggcaagagat
     2881 caaagcagtg tttacctgaa acttctcatg catgagacgc agcctccatc tcatttctcc
     2941 gtcagcacaa taaccagaat atatagaggc tataacctac taccaagcat aacagacgtc
     3001 actatggaaa ataacctttc aagagttatt gcagcagttt ctcatgaact aatcacatca
     3061 accaccagag cactcacatt tggatgctgt gaagctttgt gtcttctttc cactgccttc
     3121 ccagtttgca tttggagttt aggttggcac tgtggagtgc ctccactgag tgcctcagat
     3181 gagtctagga agagctgtac cgttgggatg gccacaatga ttctgaccct gctctcgtca
     3241 gcttggttcc cattggatct ctcagcccat caagatgctt tgattttggc cggaaacttg
     3301 cttgcagcca gtgctcccaa atctctgaga agttcatggg cctctgaaga agaagccaac
     3361 ccagcagcca ccaagcaaga ggaggtctgg ccagccctgg gggaccgggc cctggtgccc
     3421 atggtggagc agctcttctc tcacctgctg aaggtgatta acatttgtgc ccacgtcctg
     3481 gatgacgtgg ctcctggacc cgcaataaag gcagccttgc cttctctaac aaacccccct
     3541 tctctaagtc ccatccgacg aaaggggaag gagaaagaac caggagaaca agcatctgta
     3601 ccgttgagtc ccaagaaagg cagtgaggcc agtgcagctt ctagacaatc tgatacctca
     3661 ggtcctgtta caacaagtaa atcctcatca ctggggagtt tctatcatct tccttcatac
     3721 ctcaaactgc atgatgtcct gaaagctaca cacgctaact acaaggtcac gctggatctt
     3781 cagaacagca cggaaaagtt tggagggttt cttcgctcag ccttggatgt tctttctcag
     3841 atactagagc tggccacact gcaggacatt gggaagtgtg ttgaagagat cctaggatac
     3901 ctgaaatcct gctttagtcg agaaccaatg atggcaactg tttgtgttca acaattgttg
     3961 aagactctct ttggcacaaa cttggcctcc cagtttgatg gcttatcttc caaccccagc
     4021 aagtcacaag gccgagcaca gcgccttggc tcctccagtg tgaggccagg cttgtaccac
     4081 tactgcttca tggccccgta cacccacttc acccaggccc tcgctgacgc cagcctgagg
     4141 aacatggtgc aggcggagca ggagaacgac acctcgggat ggtttgatgt cctccagaaa
     4201 gtgtctaccc agttgaagac aaacctcacg agtgtcacaa agaaccgtgc agataagaat
     4261 gctattcata atcacattcg tttgtttgaa cctcttgtta taaaagcttt aaaacagtac
     4321 acgactacaa catgtgtgca gttacagaag caggttttag atttgctggc gcagctggtt
     4381 cagttacggg ttaattactg tcttctggat tcagatcagg tgtttattgg ctttgtattg
     4441 aaacagtttg aatacattga agtgggccag ttcagggaat cagaggcaat cattccaaac
     4501 atctttttct tcttggtatt actatcttat gaacgctatc attcaaaaca gatcattgga
     4561 attcctaaaa tcattcagct ctgtgatggc atcatggcca gtggaaggaa ggctgtgaca
     4621 catgccatac cggctctgca gcccatagtc cacgacctct ttgtattaag aggaacaaat
     4681 aaagctgatg caggaaaaga gcttgaaacc caaaaagagg tggtggtgtc aatgttactg
     4741 agactcatcc agtaccatca ggtgttggag atgttcattc ttgtcctgca gcagtgccac
     4801 aaggagaatg aagacaagtg gaagcgactg tctcgacaga tagctgacat catcctccca
     4861 atgttagcca aacagcagat gcacattgac tctcatgaag cccttggagt gttaaataca
     4921 ttatttgaga ttttggcccc ttcctccctc cgtccggtag acatgctttt acggagtatg
     4981 ttcgtcactc caaacacaat ggcgtccgtg agcactgttc aactgtggat atcgggaatt
     5041 ctggccattt tgagggttct gatttcccag tcaactgaag atattgttct ttctcgtatt
     5101 caggagctct ccttctctcc gtatttaatc tcctgtacag taattaatag gttaagagat
     5161 ggggacagta cttcaacgct agaagaacac agtgaaggga aacaaataaa gaatttgcca
     5221 gaagaaacat tttcaaggtt tctattacaa ctggttggta ttcttttaga agacattgtt
     5281 acaaaacagc tgaaggtgga aatgagtgag cagcaacata ctttctattg ccaggaacta
     5341 ggcacactgc taatgtgtct gatccacatc ttcaagtctg gaatgttccg gagaatcaca
     5401 gcagctgcca ctaggctgtt ccgcagtgat ggctgtggcg gcagtttcta caccctggac
     5461 agcttgaact tgcgggctcg ttccatgatc accacccacc cggccctggt gctgctctgg
     5521 tgtcagatac tgctgcttgt caaccacacc gactaccgct ggtgggcaga agtgcagcag
     5581 accccgaaaa gacacagtct gtccagcaca aagttactta gtccccagat gtctggagaa
     5641 gaggaggatt ctgacttggc agccaaactt ggaatgtgca atagagaaat agtacgaaga
     5701 ggggctctca ttctcttctg tgattatgtc tgtcagaacc tccatgactc cgagcactta
     5761 acgtggctca ttgtaaatca cattcaagat ctgatcagcc tttcccacga gcctccagta
     5821 caggacttca tcagtgccgt tcatcggaac tctgctgcca gcggcctgtt catccaggca
     5881 attcagtctc gttgtgaaaa cctttcaact ccaaccatgc tgaagaaaac tcttcagtgc
     5941 ttggagggga tccatctcag ccagtcggga gctgtgctca cgctgtatgt ggacaggctt
     6001 ctgtgcaccc ctttccgtgt gctggctcgc atggtcgaca tccttgcttg tcgccgggta
     6061 gaaatgcttc tggctgcaaa tttacagagc agcatggccc agttgccaat ggaagaactc
     6121 aacagaatcc aggaatacct tcagagcagc gggctcgctc agagacacca aaggctctat
     6181 tccctgctgg acaggtttcg tctctccacc atgcaagact cacttagtcc ctctcctcca
     6241 gtctcttccc acccgctgga cggggatggg cacgtgtcac tggaaacagt gagtccggac
     6301 aaagactggt acgttcatct tgtcaaatcc cagtgttgga ccaggtcaga ttctgcactg
     6361 ctggaaggtg cagagctggt gaatcggatt cctgctgaag atatgaatgc cttcatgatg
     6421 aactcggagt tcaacctaag cctgctagct ccatgcttaa gcctagggat gagtgaaatt
     6481 tctggtggcc agaagagtgc cctttttgaa gcagcccgtg aggtgactct ggcccgtgtg
     6541 agcggcaccg tgcagcagct ccctgctgtc catcatgtct tccagcccga gctgcctgca
     6601 gaaccggcgg cctactggag caagttgaat gatctgtttg gggatgctgc actgtatcag
     6661 tccctgccca ctctggcccg ggccctggca cagtacctgg tggtggtctc caaactgccc
     6721 agtcatttgc accttcctcc tgagaaagag aaggacattg tgaaattcgt ggtggcaacc
     6781 cttgaggccc tgtcctggca tttgatccat gagcagatcc cgctgagtct ggatctccag
     6841 gcagggctgg actgctgctg cctggccctg cagctgcctg gcctctggag cgtggtctcc
     6901 tccacagagt ttgtgaccca cgcctgctcc ctcatccact gtgtgcactt catcctggag
     6961 gccgttgcag tgcagcctgg agagcagctt cttagtccag aaagaaggac aaatacccca
     7021 aaagccatca gcgaggagga ggaggaagta gatccaaaca cacagaatcc taagtatatc
     7081 actgcagcct gtgagatggt ggcagaaatg gtggagtctc tgcagtcggt gttggccttg
     7141 ggtcataaaa ggaatagcgg cgtgccggcg tttctcacgc cattgctcag gaacatcatc
     7201 atcagcctgg cccgcctgcc ccttgtcaac agctacacac gtgtgccccc actggtgtgg
     7261 aagcttggat ggtcacccaa accgggaggg gattttggca cagcattccc tgagatcccc
     7321 gtggagttcc tccaggaaaa ggaagtcttt aaggagttca tctaccgcat caacacacta
     7381 ggctggacca gtcgtactca gtttgaagaa acttgggcca ccctccttgg tgtcctggtg
     7441 acgcagcccc tcgtgatgga gcaggaggag agcccaccag aagaagacac agagaggacc
     7501 cagatcaacg tcctggccgt gcaggccatc acctcactgg tgctcagtgc aatgactgtg
     7561 cctgtggccg gcaacccagc tgtaagctgc ttggagcagc agccccggaa caagcctctg
     7621 aaagctctcg acaccaggtt tgggaggaag ctgagcatta tcagagggat tgtggagcaa
     7681 gagattcaag caatggtttc aaagagagag aatattgcca cccatcattt atatcaggca
     7741 tgggatcctg tcccttctct gtctccggct actacaggtg ccctcatcag ccacgagaag
     7801 ctgctgctgc agatcaaccc cgagcgggag ctggggagca tgagctacaa actcggccag
     7861 gtgtccatac actccgtgtg gctagggaac agcatcacac ccctgaggga ggaggaatgg
     7921 gacgaggaag aggaggagga ggccgacgcc cctgcacctt cgtcaccacc cacgtctcca
     7981 gtcaactcca ggaaacaccg ggctggagtt gacatccact cctgttcgca gtttttgctt
     8041 gagttgtaca gccgctggat cctgccgtcc agctcagcca ggaggacccc ggccatcctg
     8101 atcagtgagg tggtcagatc ccttctagtg gtctcagact tgttcaccga gcgcaaccag
     8161 tttgagctga tgtatgtgac gctgacagaa ctgcgaaggg tgcacccttc agaagacgag
     8221 atcctcgctc agtacctggt gcctgccacc tgcaaggcag ctgccgtcct tgggatggac
     8281 aaggccgtgg cggagcctgt cagccgcctg ctggagagca cgctcaggag cagccacctg
     8341 cccagcaggg ttggagccct gcacggcatc ctctatgtgc tggagtgcga cctgctggac
     8401 gacactgcca agcagctcat cccggtcatc agcgactatc tcctctccaa cctgaaaggg
     8461 atcgcccact gcgtgaacat tcacagccag cagcacgtac tggtcatgtg tgccactgcg
     8521 ttttacctca ttgagaacta tcctctggac gtagggccgg aattttcagc atcaataata
     8581 cagatgtgtg gggtgatgct gtctggaagt gaggagtcca ccccctccat catttaccac
     8641 tgtgccctca gaggcctgga gcgcctcctg ctctctgagc agctctcccg cctggatgca
     8701 gaatcgctgg tcaagctgag tgtggacaga gtgaacgtgc acagcccgca ccgggccatg
     8761 gcggctctgg gcctgatgct cacctgcatg tacacaggaa aggagaaagt cagtccgggt
     8821 agaacttcag accctaatcc tgcagccccc gacagcgagt cagtgattgt tgctatggag
     8881 cgggtatctg ttctttttga taggatcagg aaaggctttc cttgtgaagc cagagtggtg
     8941 gccaggatcc tgccccagtt tctagacgac ttcttcccac cccaggacat catgaacaaa
     9001 gtcatcggag agtttctgtc caaccagcag ccataccccc agttcatggc caccgtggtg
     9061 tataaggtgt ttcagactct gcacagcacc gggcagtcgt ccatggtccg ggactgggtc
     9121 atgctgtccc tctccaactt cacgcagagg gccccggtcg ccatggccac gtggagcctc
     9181 tcctgcttct ttgtcagcgc gtccaccagc ccgtgggtcg cggcgatcct cccacatgtc
     9241 atcagcagga tgggcaagct ggagcaggtg gacgtgaacc ttttctgcct ggtcgccaca
     9301 gacttctaca gacaccagat agaggaggag ctcgaccgca gggccttcca gtctgtgctt
     9361 gaggtggttg cagccccagg aagcccatat caccggctgc tgacttgttt acgaaatgtc
     9421 cacaaggtca ccacctgc
//
