LOCUS       sequence-209896-        9471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8789..8798
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac cgccaccgcc gccgccgccg
      181 ccgccgcctc ctcagcttcc tcagccgccg ccgcaggcac agccgctgct gcctcagccg
      241 cagccgcccc cgccgccgcc cccgccgcca cccggcccgg ctgtggctga ggagccgctg
      301 caccgaccaa agaaagaact ttcagctacc aagaaagacc gtgtgaatca ttgtctgaca
      361 atatgtgaaa acatagtggc acagtctgtc agaaattctc cagaatttca gaaacttctg
      421 ggcatcgcta tggaactttt tctgctgtgc agtgatgacg cagagtcaga tgtcaggatg
      481 gtggctgacg aatgcctcaa caaagttatc aaagctttga tggattctaa tcttccaagg
      541 ttacagctcg agctctataa ggaaattaaa aagaatggtg cccctcggag tttgcgtgct
      601 gccctgtgga ggtttgctga gctggctcac ctggttcggc ctcagaaatg caggccttac
      661 ctggtgaacc ttctgccgtg cctgactcga acaagcaaga gacccgaaga atcagtccag
      721 gagaccttgg ctgcagctgt tcccaaaatt atggcttctt ttggcaattt tgcaaatgac
      781 aatgaaatta aggttttgtt aaaggccttc atagcgaacc tgaagtcaag ctcccccacc
      841 attcggcgga cagcggctgg atcagcagtg agcatctgcc agcactcaag aaggacacaa
      901 tatttctata gttggctact aaatgtgctc ttaggcttac tcgttcctgt cgaggatgaa
      961 cactccactc tgctgattct tggcgtgctg ctcaccctga ggtatttggt gcccttgctg
     1021 cagcagcagg tcaaggacac aagcctgaaa ggcagcttcg gagtgacaag gaaagaaatg
     1081 gaagtctctc cttctgcaga gcagcttgtc caggtttatg aactgacgtt acatcataca
     1141 cagcaccaag accacaatgt tgtgaccgga gccctggagc tgttgcagca gctcttcaga
     1201 acgcctccac ccgagcttct gcaaaccctg accgcagtcg ggggcattgg gcagctcacc
     1261 gctgctaagg aggagtctgg tggccgaagc cgtagtggga gtattgtgga acttatagct
     1321 ggagggggtt cctcatgcag ccctgtcctt tcaagaaaac aaaaaggcaa agtgctctta
     1381 ggagaagaag aagccttgga ggatgactct gaatcgagat cggatgtcag cagctctgcc
     1441 ttaacagcct cagtgaagga tgagatcagt ggagagctgg ctgcttcttc aggggtttcc
     1501 actccagggt cagcaggtca tgacatcatc acagaacagc cacggtcaca gcacacactg
     1561 caggcggact cagtggatct ggccagctgt gacttgacaa gctctgccac tgatggggat
     1621 gaggaggata tcttgagcca cagctccagc caggtcagcg ccgtcccatc tgaccctgcc
     1681 atggacctga atgatgggac ccaggcctcg tcgcccatca gcgacagctc ccagaccacc
     1741 accgaagggc ctgattcagc tgttacccct tcagacagtt ctgaaattgt gttagacggt
     1801 accgacaacc agtatttggg cctgcagatt ggacagcccc aggatgaaga tgaggaagcc
     1861 acaggtattc ttcctgatga agcctcggag gccttcagga actcttccat ggcccttcaa
     1921 caggcacatt tattgaaaaa catgagtcac tgcaggcagc cttctgacag cagtgttgat
     1981 aaatttgtgt tgagagatga agctactgaa ccgggtgatc aagaaaacaa gccttgccgc
     2041 atcaaaggtg acattggaca gtccactgat gatgactctg cacctcttgt ccattgtgtc
     2101 cgccttttat ctgcttcgtt tttgctaaca gggggaaaaa atgtgctggt tccggacagg
     2161 gatgtgaggg tcagcgtgaa ggccctggcc ctcagctgtg tgggagcagc tgtggccctc
     2221 cacccggaat ctttcttcag caaactctat aaagttcctc ttgacaccac ggaataccct
     2281 gaggaacagt atgtctcaga catcttgaac tacatcgatc atggagaccc acaggttcga
     2341 ggagccactg ccattctctg tgggaccctc atctgctcca tcctcagcag gtcccgcttc
     2401 cacgtgggag attggatggg caccattaga accctcacag gaaatacatt ttctttggcg
     2461 gattgcattc ctttgctgcg gaaaacactg aaggatgagt cttctgttac ttgcaagtta
     2521 gcttgtacag ctgtgaggaa ctgtgtcatg agtctctgca gcagcagcta cagtgagtta
     2581 ggactgcagc tgatcatcga tgtgctgact ctgaggaaca gttcctattg gctggtgagg
     2641 acagagcttc tggaaaccct tgcagagatt gacttcaggc tggtgagctt tttggaggca
     2701 aaagcagaaa acttacacag aggggctcat cattatacag ggcttttaaa actgcaagaa
     2761 cgagtgctca ataatgttgt catccatttg cttggagatg aagaccccag ggtgcgacat
     2821 gttgccgcag catcactaat taggcttgtc ccaaagctgt tttataaatg tgaccaagga
     2881 caagctgatc cagtagtggc cgtggcaaga gatcaaagca gtgtttacct gaaacttctc
     2941 atgcatgaga cgcagcctcc atctcatttc tccgtcagca caataaccag aatatataga
     3001 ggctataacc tactaccaag cataacagac gtcactatgg aaaataacct ttcaagagtt
     3061 attgcagcag tttctcatga actaatcaca tcaaccacca gagcactcac atttggatgc
     3121 tgtgaagctt tgtgtcttct ttccactgcc ttcccagttt gcatttggag tttaggttgg
     3181 cactgtggag tgcctccact gagtgcctca gatgagtcta ggaagagctg taccgttggg
     3241 atggccacaa tgattctgac cctgctctcg tcagcttggt tcccattgga tctctcagcc
     3301 catcaagatg ctttgatttt ggccggaaac ttgcttgcag ccagtgctcc caaatctctg
     3361 agaagttcat gggcctctga agaagaagcc aacccagcag ccaccaagca agaggaggtc
     3421 tggccagccc tgggggaccg ggccctggtg cccatggtgg agcagctctt ctctcacctg
     3481 ctgaaggtga ttaacatttg tgcccacgtc ctggatgacg tggctcctgg acccgcaata
     3541 aaggcagcct tgccttctct aacaaacccc ccttctctaa gtcccatccg acgaaagggg
     3601 aaggagaaag aaccaggaga acaagcatct gtaccgttga gtcccaagaa aggcagtgag
     3661 gccagtgcag cttctagaca atctgatacc tcaggtcctg ttacaacaag taaatcctca
     3721 tcactgggga gtttctatca tcttccttca tacctcaaac tgcatgatgt cctgaaagct
     3781 acacacgcta actacaaggt cacgctggat cttcagaaca gcacggaaaa gtttggaggg
     3841 tttcttcgct cagccttgga tgttctttct cagatactag agctggccac actgcaggac
     3901 attgggaagt gtgttgaaga gatcctagga tacctgaaat cctgctttag tcgagaacca
     3961 atgatggcaa ctgtttgtgt tcaacaattg ttgaagactc tctttggcac aaacttggcc
     4021 tcccagtttg atggcttatc ttccaacccc agcaagtcac aaggccgagc acagcgcctt
     4081 ggctcctcca gtgtgaggcc aggcttgtac cactactgct tcatggcccc gtacacccac
     4141 ttcacccagg ccctcgctga cgccagcctg aggaacatgg tgcaggcgga gcaggagaac
     4201 gacacctcgg gatggtttga tgtcctccag aaagtgtcta cccagttgaa gacaaacctc
     4261 acgagtgtca caaagaaccg tgcagataag aatgctattc ataatcacat tcgtttgttt
     4321 gaacctcttg ttataaaagc tttaaaacag tacacgacta caacatgtgt gcagttacag
     4381 aagcaggttt tagatttgct ggcgcagctg gttcagttac gggttaatta ctgtcttctg
     4441 gattcagatc aggtgtttat tggctttgta ttgaaacagt ttgaatacat tgaagtgggc
     4501 cagttcaggg aatcagaggc aatcattcca aacatctttt tcttcttggt attactatct
     4561 tatgaacgct atcattcaaa acagatcatt ggaattccta aaatcattca gctctgtgat
     4621 ggcatcatgg ccagtggaag gaaggctgtg acacatgcca taccggctct gcagcccata
     4681 gtccacgacc tctttgtatt aagaggaaca aataaagctg atgcaggaaa agagcttgaa
     4741 acccaaaaag aggtggtggt gtcaatgtta ctgagactca tccagtacca tcaggtgttg
     4801 gagatgttca ttcttgtcct gcagcagtgc cacaaggaga atgaagacaa gtggaagcga
     4861 ctgtctcgac agatagctga catcatcctc ccaatgttag ccaaacagca gatgcacatt
     4921 gactctcatg aagcccttgg agtgttaaat acattatttg agattttggc cccttcctcc
     4981 ctccgtccgg tagacatgct tttacggagt atgttcgtca ctccaaacac aatggcgtcc
     5041 gtgagcactg ttcaactgtg gatatcggga attctggcca ttttgagggt tctgatttcc
     5101 cagtcaactg aagatattgt tctttctcgt attcaggagc tctccttctc tccgtattta
     5161 atctcctgta cagtaattaa taggttaaga gatggggaca gtacttcaac gctagaagaa
     5221 cacagtgaag ggaaacaaat aaagaatttg ccagaagaaa cattttcaag gtttctatta
     5281 caactggttg gtattctttt agaagacatt gttacaaaac agctgaaggt ggaaatgagt
     5341 gagcagcaac atactttcta ttgccaggaa ctaggcacac tgctaatgtg tctgatccac
     5401 atcttcaagt ctggaatgtt ccggagaatc acagcagctg ccactaggct gttccgcagt
     5461 gatggctgtg gcggcagttt ctacaccctg gacagcttga acttgcgggc tcgttccatg
     5521 atcaccaccc acccggccct ggtgctgctc tggtgtcaga tactgctgct tgtcaaccac
     5581 accgactacc gctggtgggc agaagtgcag cagaccccga aaagacacag tctgtccagc
     5641 acaaagttac ttagtcccca gatgtctgga gaagaggagg attctgactt ggcagccaaa
     5701 cttggaatgt gcaatagaga aatagtacga agaggggctc tcattctctt ctgtgattat
     5761 gtctgtcaga acctccatga ctccgagcac ttaacgtggc tcattgtaaa tcacattcaa
     5821 gatctgatca gcctttccca cgagcctcca gtacaggact tcatcagtgc cgttcatcgg
     5881 aactctgctg ccagcggcct gttcatccag gcaattcagt ctcgttgtga aaacctttca
     5941 actccaacca tgctgaagaa aactcttcag tgcttggagg ggatccatct cagccagtcg
     6001 ggagctgtgc tcacgctgta tgtggacagg cttctgtgca cccctttccg tgtgctggct
     6061 cgcatggtcg acatccttgc ttgtcgccgg gtagaaatgc ttctggctgc aaatttacag
     6121 agcagcatgg cccagttgcc aatggaagaa ctcaacagaa tccaggaata ccttcagagc
     6181 agcgggctcg ctcagagaca ccaaaggctc tattccctgc tggacaggtt tcgtctctcc
     6241 accatgcaag actcacttag tccctctcct ccagtctctt cccacccgct ggacggggat
     6301 gggcacgtgt cactggaaac agtgagtccg gacaaagact ggtacgttca tcttgtcaaa
     6361 tcccagtgtt ggaccaggtc agattctgca ctgctggaag gtgcagagct ggtgaatcgg
     6421 attcctgctg aagatatgaa tgccttcatg atgaactcgg agttcaacct aagcctgcta
     6481 gctccatgct taagcctagg gatgagtgaa atttctggtg gccagaagag tgcccttttt
     6541 gaagcagccc gtgaggtgac tctggcccgt gtgagcggca ccgtgcagca gctccctgct
     6601 gtccatcatg tcttccagcc cgagctgcct gcagaaccgg cggcctactg gagcaagttg
     6661 aatgatctgt ttggggatgc tgcactgtat cagtccctgc ccactctggc ccgggccctg
     6721 gcacagtacc tggtggtggt ctccaaactg cccagtcatt tgcaccttcc tcctgagaaa
     6781 gagaaggaca ttgtgaaatt cgtggtggca acccttgagg ccctgtcctg gcatttgatc
     6841 catgagcaga tcccgctgag tctggatctc caggcagggc tggactgctg ctgcctggcc
     6901 ctgcagctgc ctggcctctg gagcgtggtc tcctccacag agtttgtgac ccacgcctgc
     6961 tccctcatcc actgtgtgca cttcatcctg gaggccgttg cagtgcagcc tggagagcag
     7021 cttcttagtc cagaaagaag gacaaatacc ccaaaagcca tcagcgagga ggaggaggaa
     7081 gtagatccaa acacacagaa tcctaagtat atcactgcag cctgtgagat ggtggcagaa
     7141 atggtggagt ctctgcagtc ggtgttggcc ttgggtcata aaaggaatag cggcgtgccg
     7201 gcgtttctca cgccattgct caggaacatc atcatcagcc tggcccgcct gccccttgtc
     7261 aacagctaca cacgtgtgcc cccactggtg tggaagcttg gatggtcacc caaaccggga
     7321 ggggattttg gcacagcatt ccctgagatc cccgtggagt tcctccagga aaaggaagtc
     7381 tttaaggagt tcatctaccg catcaacaca ctaggctgga ccagtcgtac tcagtttgaa
     7441 gaaacttggg ccaccctcct tggtgtcctg gtgacgcagc ccctcgtgat ggagcaggag
     7501 gagagcccac cagaagaaga cacagagagg acccagatca acgtcctggc cgtgcaggcc
     7561 atcacctcac tggtgctcag tgcaatgact gtgcctgtgg ccggcaaccc agctgtaagc
     7621 tgcttggagc agcagccccg gaacaagcct ctgaaagctc tcgacaccag gtttgggagg
     7681 aagctgagca ttatcagagg gattgtggag caagagattc aagcaatggt ttcaaagaga
     7741 gagaatattg ccacccatca tttatatcag gcatgggatc ctgtcccttc tctgtctccg
     7801 gctactacag gtgccctcat cagccacgag aagctgctgc tgcagatcaa ccccgagcgg
     7861 gagctgggga gcatgagcta caaactcggc caggtgtcca tacactccgt gtggctaggg
     7921 aacagcatca cacccctgag ggaggaggaa tgggacgagg aagaggagga ggaggccgac
     7981 gcccctgcac cttcgtcacc acccacgtct ccagtcaact ccaggaaaca ccgggctgga
     8041 gttgacatcc actcctgttc gcagtttttg cttgagttgt acagccgctg gatcctgccg
     8101 tccagctcag ccaggaggac cccggccatc ctgatcagtg aggtggtcag atcccttcta
     8161 gtggtctcag acttgttcac cgagcgcaac cagtttgagc tgatgtatgt gacgctgaca
     8221 gaactgcgaa gggtgcaccc ttcagaagac gagatcctcg ctcagtacct ggtgcctgcc
     8281 acctgcaagg cagctgccgt ccttgggatg gacaaggccg tggcggagcc tgtcagccgc
     8341 ctgctggaga gcacgctcag gagcagccac ctgcccagca gggttggagc cctgcacggc
     8401 atcctctatg tgctggagtg cgacctgctg gacgacactg ccaagcagct catcccggtc
     8461 atcagcgact atctcctctc caacctgaaa gggatcgccc actgcgtgaa cattcacagc
     8521 cagcagcacg tactggtcat gtgtgccact gcgttttacc tcattgagaa ctatcctctg
     8581 gacgtagggc cggaattttc agcatcaata atacagatgt gtggggtgat gctgtctgga
     8641 agtgaggagt ccaccccctc catcatttac cactgtgccc tcagaggcct ggagcgcctc
     8701 ctgctctctg agcagctctc ccgcctggat gcagaatcgc tggtcaagct gagtgtggac
     8761 agagtgaacg tgcacagccc gcaccgggcc atggcggctc tgggcctgat gctcacctgc
     8821 atgtacacag gaaaggagaa agtcagtccg ggtagaactt cagaccctaa tcctgcagcc
     8881 cccgacagcg agtcagtgat tgttgctatg gagcgggtat ctgttctttt tgataggatc
     8941 aggaaaggct ttccttgtga agccagagtg gtggccagga tcctgcccca gtttctagac
     9001 gacttcttcc caccccagga catcatgaac aaagtcatcg gagagtttct gtccaaccag
     9061 cagccatacc cccagttcat ggccaccgtg gtgtataagg tgtttcagac tctgcacagc
     9121 accgggcagt cgtccatggt ccgggactgg gtcatgctgt ccctctccaa cttcacgcag
     9181 agggccccgg tcgccatggc cacgtggagc ctctcctgct tctttgtcag cgcgtccacc
     9241 agcccgtggg tcgcggcgat cctcccacat gtcatcagca ggatgggcaa gctggagcag
     9301 gtggacgtga accttttctg cctggtcgcc acagacttct acagacacca gatagaggag
     9361 gagctcgacc gcagggcctt ccagtctgtg cttgaggtgg ttgcagcccc aggaagccca
     9421 tatcaccggc tgctgacttg tttacgaaat gtccacaagg tcaccacctg c
//
