LOCUS       sequence-209897-        9489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8807..8816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaaccg
      181 ccaccgccgc cgccgccgcc gccgcctcct cagcttcctc agccgccgcc gcaggcacag
      241 ccgctgctgc ctcagccgca gccgcccccg ccgccgcccc cgccgccacc cggcccggct
      301 gtggctgagg agccgctgca ccgaccaaag aaagaacttt cagctaccaa gaaagaccgt
      361 gtgaatcatt gtctgacaat atgtgaaaac atagtggcac agtctgtcag aaattctcca
      421 gaatttcaga aacttctggg catcgctatg gaactttttc tgctgtgcag tgatgacgca
      481 gagtcagatg tcaggatggt ggctgacgaa tgcctcaaca aagttatcaa agctttgatg
      541 gattctaatc ttccaaggtt acagctcgag ctctataagg aaattaaaaa gaatggtgcc
      601 cctcggagtt tgcgtgctgc cctgtggagg tttgctgagc tggctcacct ggttcggcct
      661 cagaaatgca ggccttacct ggtgaacctt ctgccgtgcc tgactcgaac aagcaagaga
      721 cccgaagaat cagtccagga gaccttggct gcagctgttc ccaaaattat ggcttctttt
      781 ggcaattttg caaatgacaa tgaaattaag gttttgttaa aggccttcat agcgaacctg
      841 aagtcaagct cccccaccat tcggcggaca gcggctggat cagcagtgag catctgccag
      901 cactcaagaa ggacacaata tttctatagt tggctactaa atgtgctctt aggcttactc
      961 gttcctgtcg aggatgaaca ctccactctg ctgattcttg gcgtgctgct caccctgagg
     1021 tatttggtgc ccttgctgca gcagcaggtc aaggacacaa gcctgaaagg cagcttcgga
     1081 gtgacaagga aagaaatgga agtctctcct tctgcagagc agcttgtcca ggtttatgaa
     1141 ctgacgttac atcatacaca gcaccaagac cacaatgttg tgaccggagc cctggagctg
     1201 ttgcagcagc tcttcagaac gcctccaccc gagcttctgc aaaccctgac cgcagtcggg
     1261 ggcattgggc agctcaccgc tgctaaggag gagtctggtg gccgaagccg tagtgggagt
     1321 attgtggaac ttatagctgg agggggttcc tcatgcagcc ctgtcctttc aagaaaacaa
     1381 aaaggcaaag tgctcttagg agaagaagaa gccttggagg atgactctga atcgagatcg
     1441 gatgtcagca gctctgcctt aacagcctca gtgaaggatg agatcagtgg agagctggct
     1501 gcttcttcag gggtttccac tccagggtca gcaggtcatg acatcatcac agaacagcca
     1561 cggtcacagc acacactgca ggcggactca gtggatctgg ccagctgtga cttgacaagc
     1621 tctgccactg atggggatga ggaggatatc ttgagccaca gctccagcca ggtcagcgcc
     1681 gtcccatctg accctgccat ggacctgaat gatgggaccc aggcctcgtc gcccatcagc
     1741 gacagctccc agaccaccac cgaagggcct gattcagctg ttaccccttc agacagttct
     1801 gaaattgtgt tagacggtac cgacaaccag tatttgggcc tgcagattgg acagccccag
     1861 gatgaagatg aggaagccac aggtattctt cctgatgaag cctcggaggc cttcaggaac
     1921 tcttccatgg cccttcaaca ggcacattta ttgaaaaaca tgagtcactg caggcagcct
     1981 tctgacagca gtgttgataa atttgtgttg agagatgaag ctactgaacc gggtgatcaa
     2041 gaaaacaagc cttgccgcat caaaggtgac attggacagt ccactgatga tgactctgca
     2101 cctcttgtcc attgtgtccg ccttttatct gcttcgtttt tgctaacagg gggaaaaaat
     2161 gtgctggttc cggacaggga tgtgagggtc agcgtgaagg ccctggccct cagctgtgtg
     2221 ggagcagctg tggccctcca cccggaatct ttcttcagca aactctataa agttcctctt
     2281 gacaccacgg aataccctga ggaacagtat gtctcagaca tcttgaacta catcgatcat
     2341 ggagacccac aggttcgagg agccactgcc attctctgtg ggaccctcat ctgctccatc
     2401 ctcagcaggt cccgcttcca cgtgggagat tggatgggca ccattagaac cctcacagga
     2461 aatacatttt ctttggcgga ttgcattcct ttgctgcgga aaacactgaa ggatgagtct
     2521 tctgttactt gcaagttagc ttgtacagct gtgaggaact gtgtcatgag tctctgcagc
     2581 agcagctaca gtgagttagg actgcagctg atcatcgatg tgctgactct gaggaacagt
     2641 tcctattggc tggtgaggac agagcttctg gaaacccttg cagagattga cttcaggctg
     2701 gtgagctttt tggaggcaaa agcagaaaac ttacacagag gggctcatca ttatacaggg
     2761 cttttaaaac tgcaagaacg agtgctcaat aatgttgtca tccatttgct tggagatgaa
     2821 gaccccaggg tgcgacatgt tgccgcagca tcactaatta ggcttgtccc aaagctgttt
     2881 tataaatgtg accaaggaca agctgatcca gtagtggccg tggcaagaga tcaaagcagt
     2941 gtttacctga aacttctcat gcatgagacg cagcctccat ctcatttctc cgtcagcaca
     3001 ataaccagaa tatatagagg ctataaccta ctaccaagca taacagacgt cactatggaa
     3061 aataaccttt caagagttat tgcagcagtt tctcatgaac taatcacatc aaccaccaga
     3121 gcactcacat ttggatgctg tgaagctttg tgtcttcttt ccactgcctt cccagtttgc
     3181 atttggagtt taggttggca ctgtggagtg cctccactga gtgcctcaga tgagtctagg
     3241 aagagctgta ccgttgggat ggccacaatg attctgaccc tgctctcgtc agcttggttc
     3301 ccattggatc tctcagccca tcaagatgct ttgattttgg ccggaaactt gcttgcagcc
     3361 agtgctccca aatctctgag aagttcatgg gcctctgaag aagaagccaa cccagcagcc
     3421 accaagcaag aggaggtctg gccagccctg ggggaccggg ccctggtgcc catggtggag
     3481 cagctcttct ctcacctgct gaaggtgatt aacatttgtg cccacgtcct ggatgacgtg
     3541 gctcctggac ccgcaataaa ggcagccttg ccttctctaa caaacccccc ttctctaagt
     3601 cccatccgac gaaaggggaa ggagaaagaa ccaggagaac aagcatctgt accgttgagt
     3661 cccaagaaag gcagtgaggc cagtgcagct tctagacaat ctgatacctc aggtcctgtt
     3721 acaacaagta aatcctcatc actggggagt ttctatcatc ttccttcata cctcaaactg
     3781 catgatgtcc tgaaagctac acacgctaac tacaaggtca cgctggatct tcagaacagc
     3841 acggaaaagt ttggagggtt tcttcgctca gccttggatg ttctttctca gatactagag
     3901 ctggccacac tgcaggacat tgggaagtgt gttgaagaga tcctaggata cctgaaatcc
     3961 tgctttagtc gagaaccaat gatggcaact gtttgtgttc aacaattgtt gaagactctc
     4021 tttggcacaa acttggcctc ccagtttgat ggcttatctt ccaaccccag caagtcacaa
     4081 ggccgagcac agcgccttgg ctcctccagt gtgaggccag gcttgtacca ctactgcttc
     4141 atggccccgt acacccactt cacccaggcc ctcgctgacg ccagcctgag gaacatggtg
     4201 caggcggagc aggagaacga cacctcggga tggtttgatg tcctccagaa agtgtctacc
     4261 cagttgaaga caaacctcac gagtgtcaca aagaaccgtg cagataagaa tgctattcat
     4321 aatcacattc gtttgtttga acctcttgtt ataaaagctt taaaacagta cacgactaca
     4381 acatgtgtgc agttacagaa gcaggtttta gatttgctgg cgcagctggt tcagttacgg
     4441 gttaattact gtcttctgga ttcagatcag gtgtttattg gctttgtatt gaaacagttt
     4501 gaatacattg aagtgggcca gttcagggaa tcagaggcaa tcattccaaa catctttttc
     4561 ttcttggtat tactatctta tgaacgctat cattcaaaac agatcattgg aattcctaaa
     4621 atcattcagc tctgtgatgg catcatggcc agtggaagga aggctgtgac acatgccata
     4681 ccggctctgc agcccatagt ccacgacctc tttgtattaa gaggaacaaa taaagctgat
     4741 gcaggaaaag agcttgaaac ccaaaaagag gtggtggtgt caatgttact gagactcatc
     4801 cagtaccatc aggtgttgga gatgttcatt cttgtcctgc agcagtgcca caaggagaat
     4861 gaagacaagt ggaagcgact gtctcgacag atagctgaca tcatcctccc aatgttagcc
     4921 aaacagcaga tgcacattga ctctcatgaa gcccttggag tgttaaatac attatttgag
     4981 attttggccc cttcctccct ccgtccggta gacatgcttt tacggagtat gttcgtcact
     5041 ccaaacacaa tggcgtccgt gagcactgtt caactgtgga tatcgggaat tctggccatt
     5101 ttgagggttc tgatttccca gtcaactgaa gatattgttc tttctcgtat tcaggagctc
     5161 tccttctctc cgtatttaat ctcctgtaca gtaattaata ggttaagaga tggggacagt
     5221 acttcaacgc tagaagaaca cagtgaaggg aaacaaataa agaatttgcc agaagaaaca
     5281 ttttcaaggt ttctattaca actggttggt attcttttag aagacattgt tacaaaacag
     5341 ctgaaggtgg aaatgagtga gcagcaacat actttctatt gccaggaact aggcacactg
     5401 ctaatgtgtc tgatccacat cttcaagtct ggaatgttcc ggagaatcac agcagctgcc
     5461 actaggctgt tccgcagtga tggctgtggc ggcagtttct acaccctgga cagcttgaac
     5521 ttgcgggctc gttccatgat caccacccac ccggccctgg tgctgctctg gtgtcagata
     5581 ctgctgcttg tcaaccacac cgactaccgc tggtgggcag aagtgcagca gaccccgaaa
     5641 agacacagtc tgtccagcac aaagttactt agtccccaga tgtctggaga agaggaggat
     5701 tctgacttgg cagccaaact tggaatgtgc aatagagaaa tagtacgaag aggggctctc
     5761 attctcttct gtgattatgt ctgtcagaac ctccatgact ccgagcactt aacgtggctc
     5821 attgtaaatc acattcaaga tctgatcagc ctttcccacg agcctccagt acaggacttc
     5881 atcagtgccg ttcatcggaa ctctgctgcc agcggcctgt tcatccaggc aattcagtct
     5941 cgttgtgaaa acctttcaac tccaaccatg ctgaagaaaa ctcttcagtg cttggagggg
     6001 atccatctca gccagtcggg agctgtgctc acgctgtatg tggacaggct tctgtgcacc
     6061 cctttccgtg tgctggctcg catggtcgac atccttgctt gtcgccgggt agaaatgctt
     6121 ctggctgcaa atttacagag cagcatggcc cagttgccaa tggaagaact caacagaatc
     6181 caggaatacc ttcagagcag cgggctcgct cagagacacc aaaggctcta ttccctgctg
     6241 gacaggtttc gtctctccac catgcaagac tcacttagtc cctctcctcc agtctcttcc
     6301 cacccgctgg acggggatgg gcacgtgtca ctggaaacag tgagtccgga caaagactgg
     6361 tacgttcatc ttgtcaaatc ccagtgttgg accaggtcag attctgcact gctggaaggt
     6421 gcagagctgg tgaatcggat tcctgctgaa gatatgaatg ccttcatgat gaactcggag
     6481 ttcaacctaa gcctgctagc tccatgctta agcctaggga tgagtgaaat ttctggtggc
     6541 cagaagagtg ccctttttga agcagcccgt gaggtgactc tggcccgtgt gagcggcacc
     6601 gtgcagcagc tccctgctgt ccatcatgtc ttccagcccg agctgcctgc agaaccggcg
     6661 gcctactgga gcaagttgaa tgatctgttt ggggatgctg cactgtatca gtccctgccc
     6721 actctggccc gggccctggc acagtacctg gtggtggtct ccaaactgcc cagtcatttg
     6781 caccttcctc ctgagaaaga gaaggacatt gtgaaattcg tggtggcaac ccttgaggcc
     6841 ctgtcctggc atttgatcca tgagcagatc ccgctgagtc tggatctcca ggcagggctg
     6901 gactgctgct gcctggccct gcagctgcct ggcctctgga gcgtggtctc ctccacagag
     6961 tttgtgaccc acgcctgctc cctcatccac tgtgtgcact tcatcctgga ggccgttgca
     7021 gtgcagcctg gagagcagct tcttagtcca gaaagaagga caaatacccc aaaagccatc
     7081 agcgaggagg aggaggaagt agatccaaac acacagaatc ctaagtatat cactgcagcc
     7141 tgtgagatgg tggcagaaat ggtggagtct ctgcagtcgg tgttggcctt gggtcataaa
     7201 aggaatagcg gcgtgccggc gtttctcacg ccattgctca ggaacatcat catcagcctg
     7261 gcccgcctgc cccttgtcaa cagctacaca cgtgtgcccc cactggtgtg gaagcttgga
     7321 tggtcaccca aaccgggagg ggattttggc acagcattcc ctgagatccc cgtggagttc
     7381 ctccaggaaa aggaagtctt taaggagttc atctaccgca tcaacacact aggctggacc
     7441 agtcgtactc agtttgaaga aacttgggcc accctccttg gtgtcctggt gacgcagccc
     7501 ctcgtgatgg agcaggagga gagcccacca gaagaagaca cagagaggac ccagatcaac
     7561 gtcctggccg tgcaggccat cacctcactg gtgctcagtg caatgactgt gcctgtggcc
     7621 ggcaacccag ctgtaagctg cttggagcag cagccccgga acaagcctct gaaagctctc
     7681 gacaccaggt ttgggaggaa gctgagcatt atcagaggga ttgtggagca agagattcaa
     7741 gcaatggttt caaagagaga gaatattgcc acccatcatt tatatcaggc atgggatcct
     7801 gtcccttctc tgtctccggc tactacaggt gccctcatca gccacgagaa gctgctgctg
     7861 cagatcaacc ccgagcggga gctggggagc atgagctaca aactcggcca ggtgtccata
     7921 cactccgtgt ggctagggaa cagcatcaca cccctgaggg aggaggaatg ggacgaggaa
     7981 gaggaggagg aggccgacgc ccctgcacct tcgtcaccac ccacgtctcc agtcaactcc
     8041 aggaaacacc gggctggagt tgacatccac tcctgttcgc agtttttgct tgagttgtac
     8101 agccgctgga tcctgccgtc cagctcagcc aggaggaccc cggccatcct gatcagtgag
     8161 gtggtcagat cccttctagt ggtctcagac ttgttcaccg agcgcaacca gtttgagctg
     8221 atgtatgtga cgctgacaga actgcgaagg gtgcaccctt cagaagacga gatcctcgct
     8281 cagtacctgg tgcctgccac ctgcaaggca gctgccgtcc ttgggatgga caaggccgtg
     8341 gcggagcctg tcagccgcct gctggagagc acgctcagga gcagccacct gcccagcagg
     8401 gttggagccc tgcacggcat cctctatgtg ctggagtgcg acctgctgga cgacactgcc
     8461 aagcagctca tcccggtcat cagcgactat ctcctctcca acctgaaagg gatcgcccac
     8521 tgcgtgaaca ttcacagcca gcagcacgta ctggtcatgt gtgccactgc gttttacctc
     8581 attgagaact atcctctgga cgtagggccg gaattttcag catcaataat acagatgtgt
     8641 ggggtgatgc tgtctggaag tgaggagtcc accccctcca tcatttacca ctgtgccctc
     8701 agaggcctgg agcgcctcct gctctctgag cagctctccc gcctggatgc agaatcgctg
     8761 gtcaagctga gtgtggacag agtgaacgtg cacagcccgc accgggccat ggcggctctg
     8821 ggcctgatgc tcacctgcat gtacacagga aaggagaaag tcagtccggg tagaacttca
     8881 gaccctaatc ctgcagcccc cgacagcgag tcagtgattg ttgctatgga gcgggtatct
     8941 gttctttttg ataggatcag gaaaggcttt ccttgtgaag ccagagtggt ggccaggatc
     9001 ctgccccagt ttctagacga cttcttccca ccccaggaca tcatgaacaa agtcatcgga
     9061 gagtttctgt ccaaccagca gccatacccc cagttcatgg ccaccgtggt gtataaggtg
     9121 tttcagactc tgcacagcac cgggcagtcg tccatggtcc gggactgggt catgctgtcc
     9181 ctctccaact tcacgcagag ggccccggtc gccatggcca cgtggagcct ctcctgcttc
     9241 tttgtcagcg cgtccaccag cccgtgggtc gcggcgatcc tcccacatgt catcagcagg
     9301 atgggcaagc tggagcaggt ggacgtgaac cttttctgcc tggtcgccac agacttctac
     9361 agacaccaga tagaggagga gctcgaccgc agggccttcc agtctgtgct tgaggtggtt
     9421 gcagccccag gaagcccata tcaccggctg ctgacttgtt tacgaaatgt ccacaaggtc
     9481 accacctgc
//
