LOCUS       sequence-209898-        9507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8825..8834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaaccgcc accgccgccg ccgccgccgc cgcctcctca gcttcctcag
      241 ccgccgccgc aggcacagcc gctgctgcct cagccgcagc cgcccccgcc gccgcccccg
      301 ccgccacccg gcccggctgt ggctgaggag ccgctgcacc gaccaaagaa agaactttca
      361 gctaccaaga aagaccgtgt gaatcattgt ctgacaatat gtgaaaacat agtggcacag
      421 tctgtcagaa attctccaga atttcagaaa cttctgggca tcgctatgga actttttctg
      481 ctgtgcagtg atgacgcaga gtcagatgtc aggatggtgg ctgacgaatg cctcaacaaa
      541 gttatcaaag ctttgatgga ttctaatctt ccaaggttac agctcgagct ctataaggaa
      601 attaaaaaga atggtgcccc tcggagtttg cgtgctgccc tgtggaggtt tgctgagctg
      661 gctcacctgg ttcggcctca gaaatgcagg ccttacctgg tgaaccttct gccgtgcctg
      721 actcgaacaa gcaagagacc cgaagaatca gtccaggaga ccttggctgc agctgttccc
      781 aaaattatgg cttcttttgg caattttgca aatgacaatg aaattaaggt tttgttaaag
      841 gccttcatag cgaacctgaa gtcaagctcc cccaccattc ggcggacagc ggctggatca
      901 gcagtgagca tctgccagca ctcaagaagg acacaatatt tctatagttg gctactaaat
      961 gtgctcttag gcttactcgt tcctgtcgag gatgaacact ccactctgct gattcttggc
     1021 gtgctgctca ccctgaggta tttggtgccc ttgctgcagc agcaggtcaa ggacacaagc
     1081 ctgaaaggca gcttcggagt gacaaggaaa gaaatggaag tctctccttc tgcagagcag
     1141 cttgtccagg tttatgaact gacgttacat catacacagc accaagacca caatgttgtg
     1201 accggagccc tggagctgtt gcagcagctc ttcagaacgc ctccacccga gcttctgcaa
     1261 accctgaccg cagtcggggg cattgggcag ctcaccgctg ctaaggagga gtctggtggc
     1321 cgaagccgta gtgggagtat tgtggaactt atagctggag ggggttcctc atgcagccct
     1381 gtcctttcaa gaaaacaaaa aggcaaagtg ctcttaggag aagaagaagc cttggaggat
     1441 gactctgaat cgagatcgga tgtcagcagc tctgccttaa cagcctcagt gaaggatgag
     1501 atcagtggag agctggctgc ttcttcaggg gtttccactc cagggtcagc aggtcatgac
     1561 atcatcacag aacagccacg gtcacagcac acactgcagg cggactcagt ggatctggcc
     1621 agctgtgact tgacaagctc tgccactgat ggggatgagg aggatatctt gagccacagc
     1681 tccagccagg tcagcgccgt cccatctgac cctgccatgg acctgaatga tgggacccag
     1741 gcctcgtcgc ccatcagcga cagctcccag accaccaccg aagggcctga ttcagctgtt
     1801 accccttcag acagttctga aattgtgtta gacggtaccg acaaccagta tttgggcctg
     1861 cagattggac agccccagga tgaagatgag gaagccacag gtattcttcc tgatgaagcc
     1921 tcggaggcct tcaggaactc ttccatggcc cttcaacagg cacatttatt gaaaaacatg
     1981 agtcactgca ggcagccttc tgacagcagt gttgataaat ttgtgttgag agatgaagct
     2041 actgaaccgg gtgatcaaga aaacaagcct tgccgcatca aaggtgacat tggacagtcc
     2101 actgatgatg actctgcacc tcttgtccat tgtgtccgcc ttttatctgc ttcgtttttg
     2161 ctaacagggg gaaaaaatgt gctggttccg gacagggatg tgagggtcag cgtgaaggcc
     2221 ctggccctca gctgtgtggg agcagctgtg gccctccacc cggaatcttt cttcagcaaa
     2281 ctctataaag ttcctcttga caccacggaa taccctgagg aacagtatgt ctcagacatc
     2341 ttgaactaca tcgatcatgg agacccacag gttcgaggag ccactgccat tctctgtggg
     2401 accctcatct gctccatcct cagcaggtcc cgcttccacg tgggagattg gatgggcacc
     2461 attagaaccc tcacaggaaa tacattttct ttggcggatt gcattccttt gctgcggaaa
     2521 acactgaagg atgagtcttc tgttacttgc aagttagctt gtacagctgt gaggaactgt
     2581 gtcatgagtc tctgcagcag cagctacagt gagttaggac tgcagctgat catcgatgtg
     2641 ctgactctga ggaacagttc ctattggctg gtgaggacag agcttctgga aacccttgca
     2701 gagattgact tcaggctggt gagctttttg gaggcaaaag cagaaaactt acacagaggg
     2761 gctcatcatt atacagggct tttaaaactg caagaacgag tgctcaataa tgttgtcatc
     2821 catttgcttg gagatgaaga ccccagggtg cgacatgttg ccgcagcatc actaattagg
     2881 cttgtcccaa agctgtttta taaatgtgac caaggacaag ctgatccagt agtggccgtg
     2941 gcaagagatc aaagcagtgt ttacctgaaa cttctcatgc atgagacgca gcctccatct
     3001 catttctccg tcagcacaat aaccagaata tatagaggct ataacctact accaagcata
     3061 acagacgtca ctatggaaaa taacctttca agagttattg cagcagtttc tcatgaacta
     3121 atcacatcaa ccaccagagc actcacattt ggatgctgtg aagctttgtg tcttctttcc
     3181 actgccttcc cagtttgcat ttggagttta ggttggcact gtggagtgcc tccactgagt
     3241 gcctcagatg agtctaggaa gagctgtacc gttgggatgg ccacaatgat tctgaccctg
     3301 ctctcgtcag cttggttccc attggatctc tcagcccatc aagatgcttt gattttggcc
     3361 ggaaacttgc ttgcagccag tgctcccaaa tctctgagaa gttcatgggc ctctgaagaa
     3421 gaagccaacc cagcagccac caagcaagag gaggtctggc cagccctggg ggaccgggcc
     3481 ctggtgccca tggtggagca gctcttctct cacctgctga aggtgattaa catttgtgcc
     3541 cacgtcctgg atgacgtggc tcctggaccc gcaataaagg cagccttgcc ttctctaaca
     3601 aacccccctt ctctaagtcc catccgacga aaggggaagg agaaagaacc aggagaacaa
     3661 gcatctgtac cgttgagtcc caagaaaggc agtgaggcca gtgcagcttc tagacaatct
     3721 gatacctcag gtcctgttac aacaagtaaa tcctcatcac tggggagttt ctatcatctt
     3781 ccttcatacc tcaaactgca tgatgtcctg aaagctacac acgctaacta caaggtcacg
     3841 ctggatcttc agaacagcac ggaaaagttt ggagggtttc ttcgctcagc cttggatgtt
     3901 ctttctcaga tactagagct ggccacactg caggacattg ggaagtgtgt tgaagagatc
     3961 ctaggatacc tgaaatcctg ctttagtcga gaaccaatga tggcaactgt ttgtgttcaa
     4021 caattgttga agactctctt tggcacaaac ttggcctccc agtttgatgg cttatcttcc
     4081 aaccccagca agtcacaagg ccgagcacag cgccttggct cctccagtgt gaggccaggc
     4141 ttgtaccact actgcttcat ggccccgtac acccacttca cccaggccct cgctgacgcc
     4201 agcctgagga acatggtgca ggcggagcag gagaacgaca cctcgggatg gtttgatgtc
     4261 ctccagaaag tgtctaccca gttgaagaca aacctcacga gtgtcacaaa gaaccgtgca
     4321 gataagaatg ctattcataa tcacattcgt ttgtttgaac ctcttgttat aaaagcttta
     4381 aaacagtaca cgactacaac atgtgtgcag ttacagaagc aggttttaga tttgctggcg
     4441 cagctggttc agttacgggt taattactgt cttctggatt cagatcaggt gtttattggc
     4501 tttgtattga aacagtttga atacattgaa gtgggccagt tcagggaatc agaggcaatc
     4561 attccaaaca tctttttctt cttggtatta ctatcttatg aacgctatca ttcaaaacag
     4621 atcattggaa ttcctaaaat cattcagctc tgtgatggca tcatggccag tggaaggaag
     4681 gctgtgacac atgccatacc ggctctgcag cccatagtcc acgacctctt tgtattaaga
     4741 ggaacaaata aagctgatgc aggaaaagag cttgaaaccc aaaaagaggt ggtggtgtca
     4801 atgttactga gactcatcca gtaccatcag gtgttggaga tgttcattct tgtcctgcag
     4861 cagtgccaca aggagaatga agacaagtgg aagcgactgt ctcgacagat agctgacatc
     4921 atcctcccaa tgttagccaa acagcagatg cacattgact ctcatgaagc ccttggagtg
     4981 ttaaatacat tatttgagat tttggcccct tcctccctcc gtccggtaga catgctttta
     5041 cggagtatgt tcgtcactcc aaacacaatg gcgtccgtga gcactgttca actgtggata
     5101 tcgggaattc tggccatttt gagggttctg atttcccagt caactgaaga tattgttctt
     5161 tctcgtattc aggagctctc cttctctccg tatttaatct cctgtacagt aattaatagg
     5221 ttaagagatg gggacagtac ttcaacgcta gaagaacaca gtgaagggaa acaaataaag
     5281 aatttgccag aagaaacatt ttcaaggttt ctattacaac tggttggtat tcttttagaa
     5341 gacattgtta caaaacagct gaaggtggaa atgagtgagc agcaacatac tttctattgc
     5401 caggaactag gcacactgct aatgtgtctg atccacatct tcaagtctgg aatgttccgg
     5461 agaatcacag cagctgccac taggctgttc cgcagtgatg gctgtggcgg cagtttctac
     5521 accctggaca gcttgaactt gcgggctcgt tccatgatca ccacccaccc ggccctggtg
     5581 ctgctctggt gtcagatact gctgcttgtc aaccacaccg actaccgctg gtgggcagaa
     5641 gtgcagcaga ccccgaaaag acacagtctg tccagcacaa agttacttag tccccagatg
     5701 tctggagaag aggaggattc tgacttggca gccaaacttg gaatgtgcaa tagagaaata
     5761 gtacgaagag gggctctcat tctcttctgt gattatgtct gtcagaacct ccatgactcc
     5821 gagcacttaa cgtggctcat tgtaaatcac attcaagatc tgatcagcct ttcccacgag
     5881 cctccagtac aggacttcat cagtgccgtt catcggaact ctgctgccag cggcctgttc
     5941 atccaggcaa ttcagtctcg ttgtgaaaac ctttcaactc caaccatgct gaagaaaact
     6001 cttcagtgct tggaggggat ccatctcagc cagtcgggag ctgtgctcac gctgtatgtg
     6061 gacaggcttc tgtgcacccc tttccgtgtg ctggctcgca tggtcgacat ccttgcttgt
     6121 cgccgggtag aaatgcttct ggctgcaaat ttacagagca gcatggccca gttgccaatg
     6181 gaagaactca acagaatcca ggaatacctt cagagcagcg ggctcgctca gagacaccaa
     6241 aggctctatt ccctgctgga caggtttcgt ctctccacca tgcaagactc acttagtccc
     6301 tctcctccag tctcttccca cccgctggac ggggatgggc acgtgtcact ggaaacagtg
     6361 agtccggaca aagactggta cgttcatctt gtcaaatccc agtgttggac caggtcagat
     6421 tctgcactgc tggaaggtgc agagctggtg aatcggattc ctgctgaaga tatgaatgcc
     6481 ttcatgatga actcggagtt caacctaagc ctgctagctc catgcttaag cctagggatg
     6541 agtgaaattt ctggtggcca gaagagtgcc ctttttgaag cagcccgtga ggtgactctg
     6601 gcccgtgtga gcggcaccgt gcagcagctc cctgctgtcc atcatgtctt ccagcccgag
     6661 ctgcctgcag aaccggcggc ctactggagc aagttgaatg atctgtttgg ggatgctgca
     6721 ctgtatcagt ccctgcccac tctggcccgg gccctggcac agtacctggt ggtggtctcc
     6781 aaactgccca gtcatttgca ccttcctcct gagaaagaga aggacattgt gaaattcgtg
     6841 gtggcaaccc ttgaggccct gtcctggcat ttgatccatg agcagatccc gctgagtctg
     6901 gatctccagg cagggctgga ctgctgctgc ctggccctgc agctgcctgg cctctggagc
     6961 gtggtctcct ccacagagtt tgtgacccac gcctgctccc tcatccactg tgtgcacttc
     7021 atcctggagg ccgttgcagt gcagcctgga gagcagcttc ttagtccaga aagaaggaca
     7081 aataccccaa aagccatcag cgaggaggag gaggaagtag atccaaacac acagaatcct
     7141 aagtatatca ctgcagcctg tgagatggtg gcagaaatgg tggagtctct gcagtcggtg
     7201 ttggccttgg gtcataaaag gaatagcggc gtgccggcgt ttctcacgcc attgctcagg
     7261 aacatcatca tcagcctggc ccgcctgccc cttgtcaaca gctacacacg tgtgccccca
     7321 ctggtgtgga agcttggatg gtcacccaaa ccgggagggg attttggcac agcattccct
     7381 gagatccccg tggagttcct ccaggaaaag gaagtcttta aggagttcat ctaccgcatc
     7441 aacacactag gctggaccag tcgtactcag tttgaagaaa cttgggccac cctccttggt
     7501 gtcctggtga cgcagcccct cgtgatggag caggaggaga gcccaccaga agaagacaca
     7561 gagaggaccc agatcaacgt cctggccgtg caggccatca cctcactggt gctcagtgca
     7621 atgactgtgc ctgtggccgg caacccagct gtaagctgct tggagcagca gccccggaac
     7681 aagcctctga aagctctcga caccaggttt gggaggaagc tgagcattat cagagggatt
     7741 gtggagcaag agattcaagc aatggtttca aagagagaga atattgccac ccatcattta
     7801 tatcaggcat gggatcctgt cccttctctg tctccggcta ctacaggtgc cctcatcagc
     7861 cacgagaagc tgctgctgca gatcaacccc gagcgggagc tggggagcat gagctacaaa
     7921 ctcggccagg tgtccataca ctccgtgtgg ctagggaaca gcatcacacc cctgagggag
     7981 gaggaatggg acgaggaaga ggaggaggag gccgacgccc ctgcaccttc gtcaccaccc
     8041 acgtctccag tcaactccag gaaacaccgg gctggagttg acatccactc ctgttcgcag
     8101 tttttgcttg agttgtacag ccgctggatc ctgccgtcca gctcagccag gaggaccccg
     8161 gccatcctga tcagtgaggt ggtcagatcc cttctagtgg tctcagactt gttcaccgag
     8221 cgcaaccagt ttgagctgat gtatgtgacg ctgacagaac tgcgaagggt gcacccttca
     8281 gaagacgaga tcctcgctca gtacctggtg cctgccacct gcaaggcagc tgccgtcctt
     8341 gggatggaca aggccgtggc ggagcctgtc agccgcctgc tggagagcac gctcaggagc
     8401 agccacctgc ccagcagggt tggagccctg cacggcatcc tctatgtgct ggagtgcgac
     8461 ctgctggacg acactgccaa gcagctcatc ccggtcatca gcgactatct cctctccaac
     8521 ctgaaaggga tcgcccactg cgtgaacatt cacagccagc agcacgtact ggtcatgtgt
     8581 gccactgcgt tttacctcat tgagaactat cctctggacg tagggccgga attttcagca
     8641 tcaataatac agatgtgtgg ggtgatgctg tctggaagtg aggagtccac cccctccatc
     8701 atttaccact gtgccctcag aggcctggag cgcctcctgc tctctgagca gctctcccgc
     8761 ctggatgcag aatcgctggt caagctgagt gtggacagag tgaacgtgca cagcccgcac
     8821 cgggccatgg cggctctggg cctgatgctc acctgcatgt acacaggaaa ggagaaagtc
     8881 agtccgggta gaacttcaga ccctaatcct gcagcccccg acagcgagtc agtgattgtt
     8941 gctatggagc gggtatctgt tctttttgat aggatcagga aaggctttcc ttgtgaagcc
     9001 agagtggtgg ccaggatcct gccccagttt ctagacgact tcttcccacc ccaggacatc
     9061 atgaacaaag tcatcggaga gtttctgtcc aaccagcagc cataccccca gttcatggcc
     9121 accgtggtgt ataaggtgtt tcagactctg cacagcaccg ggcagtcgtc catggtccgg
     9181 gactgggtca tgctgtccct ctccaacttc acgcagaggg ccccggtcgc catggccacg
     9241 tggagcctct cctgcttctt tgtcagcgcg tccaccagcc cgtgggtcgc ggcgatcctc
     9301 ccacatgtca tcagcaggat gggcaagctg gagcaggtgg acgtgaacct tttctgcctg
     9361 gtcgccacag acttctacag acaccagata gaggaggagc tcgaccgcag ggccttccag
     9421 tctgtgcttg aggtggttgc agccccagga agcccatatc accggctgct gacttgttta
     9481 cgaaatgtcc acaaggtcac cacctgc
//
