LOCUS       sequence-209899-        9519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8837..8846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcaa caacagcagc aacagcaaca acagcagcaa
      181 cagcagcaac agcagcaaca gcaacaaccg ccaccgccgc cgccgccgcc gccgcctcct
      241 cagcttcctc agccgccgcc gcaggcacag ccgctgctgc ctcagccgca gccgcccccg
      301 ccgccgcccc cgccgccacc cggcccggct gtggctgagg agccgctgca ccgaccaaag
      361 aaagaacttt cagctaccaa gaaagaccgt gtgaatcatt gtctgacaat atgtgaaaac
      421 atagtggcac agtctgtcag aaattctcca gaatttcaga aacttctggg catcgctatg
      481 gaactttttc tgctgtgcag tgatgacgca gagtcagatg tcaggatggt ggctgacgaa
      541 tgcctcaaca aagttatcaa agctttgatg gattctaatc ttccaaggtt acagctcgag
      601 ctctataagg aaattaaaaa gaatggtgcc cctcggagtt tgcgtgctgc cctgtggagg
      661 tttgctgagc tggctcacct ggttcggcct cagaaatgca ggccttacct ggtgaacctt
      721 ctgccgtgcc tgactcgaac aagcaagaga cccgaagaat cagtccagga gaccttggct
      781 gcagctgttc ccaaaattat ggcttctttt ggcaattttg caaatgacaa tgaaattaag
      841 gttttgttaa aggccttcat agcgaacctg aagtcaagct cccccaccat tcggcggaca
      901 gcggctggat cagcagtgag catctgccag cactcaagaa ggacacaata tttctatagt
      961 tggctactaa atgtgctctt aggcttactc gttcctgtcg aggatgaaca ctccactctg
     1021 ctgattcttg gcgtgctgct caccctgagg tatttggtgc ccttgctgca gcagcaggtc
     1081 aaggacacaa gcctgaaagg cagcttcgga gtgacaagga aagaaatgga agtctctcct
     1141 tctgcagagc agcttgtcca ggtttatgaa ctgacgttac atcatacaca gcaccaagac
     1201 cacaatgttg tgaccggagc cctggagctg ttgcagcagc tcttcagaac gcctccaccc
     1261 gagcttctgc aaaccctgac cgcagtcggg ggcattgggc agctcaccgc tgctaaggag
     1321 gagtctggtg gccgaagccg tagtgggagt attgtggaac ttatagctgg agggggttcc
     1381 tcatgcagcc ctgtcctttc aagaaaacaa aaaggcaaag tgctcttagg agaagaagaa
     1441 gccttggagg atgactctga atcgagatcg gatgtcagca gctctgcctt aacagcctca
     1501 gtgaaggatg agatcagtgg agagctggct gcttcttcag gggtttccac tccagggtca
     1561 gcaggtcatg acatcatcac agaacagcca cggtcacagc acacactgca ggcggactca
     1621 gtggatctgg ccagctgtga cttgacaagc tctgccactg atggggatga ggaggatatc
     1681 ttgagccaca gctccagcca ggtcagcgcc gtcccatctg accctgccat ggacctgaat
     1741 gatgggaccc aggcctcgtc gcccatcagc gacagctccc agaccaccac cgaagggcct
     1801 gattcagctg ttaccccttc agacagttct gaaattgtgt tagacggtac cgacaaccag
     1861 tatttgggcc tgcagattgg acagccccag gatgaagatg aggaagccac aggtattctt
     1921 cctgatgaag cctcggaggc cttcaggaac tcttccatgg cccttcaaca ggcacattta
     1981 ttgaaaaaca tgagtcactg caggcagcct tctgacagca gtgttgataa atttgtgttg
     2041 agagatgaag ctactgaacc gggtgatcaa gaaaacaagc cttgccgcat caaaggtgac
     2101 attggacagt ccactgatga tgactctgca cctcttgtcc attgtgtccg ccttttatct
     2161 gcttcgtttt tgctaacagg gggaaaaaat gtgctggttc cggacaggga tgtgagggtc
     2221 agcgtgaagg ccctggccct cagctgtgtg ggagcagctg tggccctcca cccggaatct
     2281 ttcttcagca aactctataa agttcctctt gacaccacgg aataccctga ggaacagtat
     2341 gtctcagaca tcttgaacta catcgatcat ggagacccac aggttcgagg agccactgcc
     2401 attctctgtg ggaccctcat ctgctccatc ctcagcaggt cccgcttcca cgtgggagat
     2461 tggatgggca ccattagaac cctcacagga aatacatttt ctttggcgga ttgcattcct
     2521 ttgctgcgga aaacactgaa ggatgagtct tctgttactt gcaagttagc ttgtacagct
     2581 gtgaggaact gtgtcatgag tctctgcagc agcagctaca gtgagttagg actgcagctg
     2641 atcatcgatg tgctgactct gaggaacagt tcctattggc tggtgaggac agagcttctg
     2701 gaaacccttg cagagattga cttcaggctg gtgagctttt tggaggcaaa agcagaaaac
     2761 ttacacagag gggctcatca ttatacaggg cttttaaaac tgcaagaacg agtgctcaat
     2821 aatgttgtca tccatttgct tggagatgaa gaccccaggg tgcgacatgt tgccgcagca
     2881 tcactaatta ggcttgtccc aaagctgttt tataaatgtg accaaggaca agctgatcca
     2941 gtagtggccg tggcaagaga tcaaagcagt gtttacctga aacttctcat gcatgagacg
     3001 cagcctccat ctcatttctc cgtcagcaca ataaccagaa tatatagagg ctataaccta
     3061 ctaccaagca taacagacgt cactatggaa aataaccttt caagagttat tgcagcagtt
     3121 tctcatgaac taatcacatc aaccaccaga gcactcacat ttggatgctg tgaagctttg
     3181 tgtcttcttt ccactgcctt cccagtttgc atttggagtt taggttggca ctgtggagtg
     3241 cctccactga gtgcctcaga tgagtctagg aagagctgta ccgttgggat ggccacaatg
     3301 attctgaccc tgctctcgtc agcttggttc ccattggatc tctcagccca tcaagatgct
     3361 ttgattttgg ccggaaactt gcttgcagcc agtgctccca aatctctgag aagttcatgg
     3421 gcctctgaag aagaagccaa cccagcagcc accaagcaag aggaggtctg gccagccctg
     3481 ggggaccggg ccctggtgcc catggtggag cagctcttct ctcacctgct gaaggtgatt
     3541 aacatttgtg cccacgtcct ggatgacgtg gctcctggac ccgcaataaa ggcagccttg
     3601 ccttctctaa caaacccccc ttctctaagt cccatccgac gaaaggggaa ggagaaagaa
     3661 ccaggagaac aagcatctgt accgttgagt cccaagaaag gcagtgaggc cagtgcagct
     3721 tctagacaat ctgatacctc aggtcctgtt acaacaagta aatcctcatc actggggagt
     3781 ttctatcatc ttccttcata cctcaaactg catgatgtcc tgaaagctac acacgctaac
     3841 tacaaggtca cgctggatct tcagaacagc acggaaaagt ttggagggtt tcttcgctca
     3901 gccttggatg ttctttctca gatactagag ctggccacac tgcaggacat tgggaagtgt
     3961 gttgaagaga tcctaggata cctgaaatcc tgctttagtc gagaaccaat gatggcaact
     4021 gtttgtgttc aacaattgtt gaagactctc tttggcacaa acttggcctc ccagtttgat
     4081 ggcttatctt ccaaccccag caagtcacaa ggccgagcac agcgccttgg ctcctccagt
     4141 gtgaggccag gcttgtacca ctactgcttc atggccccgt acacccactt cacccaggcc
     4201 ctcgctgacg ccagcctgag gaacatggtg caggcggagc aggagaacga cacctcggga
     4261 tggtttgatg tcctccagaa agtgtctacc cagttgaaga caaacctcac gagtgtcaca
     4321 aagaaccgtg cagataagaa tgctattcat aatcacattc gtttgtttga acctcttgtt
     4381 ataaaagctt taaaacagta cacgactaca acatgtgtgc agttacagaa gcaggtttta
     4441 gatttgctgg cgcagctggt tcagttacgg gttaattact gtcttctgga ttcagatcag
     4501 gtgtttattg gctttgtatt gaaacagttt gaatacattg aagtgggcca gttcagggaa
     4561 tcagaggcaa tcattccaaa catctttttc ttcttggtat tactatctta tgaacgctat
     4621 cattcaaaac agatcattgg aattcctaaa atcattcagc tctgtgatgg catcatggcc
     4681 agtggaagga aggctgtgac acatgccata ccggctctgc agcccatagt ccacgacctc
     4741 tttgtattaa gaggaacaaa taaagctgat gcaggaaaag agcttgaaac ccaaaaagag
     4801 gtggtggtgt caatgttact gagactcatc cagtaccatc aggtgttgga gatgttcatt
     4861 cttgtcctgc agcagtgcca caaggagaat gaagacaagt ggaagcgact gtctcgacag
     4921 atagctgaca tcatcctccc aatgttagcc aaacagcaga tgcacattga ctctcatgaa
     4981 gcccttggag tgttaaatac attatttgag attttggccc cttcctccct ccgtccggta
     5041 gacatgcttt tacggagtat gttcgtcact ccaaacacaa tggcgtccgt gagcactgtt
     5101 caactgtgga tatcgggaat tctggccatt ttgagggttc tgatttccca gtcaactgaa
     5161 gatattgttc tttctcgtat tcaggagctc tccttctctc cgtatttaat ctcctgtaca
     5221 gtaattaata ggttaagaga tggggacagt acttcaacgc tagaagaaca cagtgaaggg
     5281 aaacaaataa agaatttgcc agaagaaaca ttttcaaggt ttctattaca actggttggt
     5341 attcttttag aagacattgt tacaaaacag ctgaaggtgg aaatgagtga gcagcaacat
     5401 actttctatt gccaggaact aggcacactg ctaatgtgtc tgatccacat cttcaagtct
     5461 ggaatgttcc ggagaatcac agcagctgcc actaggctgt tccgcagtga tggctgtggc
     5521 ggcagtttct acaccctgga cagcttgaac ttgcgggctc gttccatgat caccacccac
     5581 ccggccctgg tgctgctctg gtgtcagata ctgctgcttg tcaaccacac cgactaccgc
     5641 tggtgggcag aagtgcagca gaccccgaaa agacacagtc tgtccagcac aaagttactt
     5701 agtccccaga tgtctggaga agaggaggat tctgacttgg cagccaaact tggaatgtgc
     5761 aatagagaaa tagtacgaag aggggctctc attctcttct gtgattatgt ctgtcagaac
     5821 ctccatgact ccgagcactt aacgtggctc attgtaaatc acattcaaga tctgatcagc
     5881 ctttcccacg agcctccagt acaggacttc atcagtgccg ttcatcggaa ctctgctgcc
     5941 agcggcctgt tcatccaggc aattcagtct cgttgtgaaa acctttcaac tccaaccatg
     6001 ctgaagaaaa ctcttcagtg cttggagggg atccatctca gccagtcggg agctgtgctc
     6061 acgctgtatg tggacaggct tctgtgcacc cctttccgtg tgctggctcg catggtcgac
     6121 atccttgctt gtcgccgggt agaaatgctt ctggctgcaa atttacagag cagcatggcc
     6181 cagttgccaa tggaagaact caacagaatc caggaatacc ttcagagcag cgggctcgct
     6241 cagagacacc aaaggctcta ttccctgctg gacaggtttc gtctctccac catgcaagac
     6301 tcacttagtc cctctcctcc agtctcttcc cacccgctgg acggggatgg gcacgtgtca
     6361 ctggaaacag tgagtccgga caaagactgg tacgttcatc ttgtcaaatc ccagtgttgg
     6421 accaggtcag attctgcact gctggaaggt gcagagctgg tgaatcggat tcctgctgaa
     6481 gatatgaatg ccttcatgat gaactcggag ttcaacctaa gcctgctagc tccatgctta
     6541 agcctaggga tgagtgaaat ttctggtggc cagaagagtg ccctttttga agcagcccgt
     6601 gaggtgactc tggcccgtgt gagcggcacc gtgcagcagc tccctgctgt ccatcatgtc
     6661 ttccagcccg agctgcctgc agaaccggcg gcctactgga gcaagttgaa tgatctgttt
     6721 ggggatgctg cactgtatca gtccctgccc actctggccc gggccctggc acagtacctg
     6781 gtggtggtct ccaaactgcc cagtcatttg caccttcctc ctgagaaaga gaaggacatt
     6841 gtgaaattcg tggtggcaac ccttgaggcc ctgtcctggc atttgatcca tgagcagatc
     6901 ccgctgagtc tggatctcca ggcagggctg gactgctgct gcctggccct gcagctgcct
     6961 ggcctctgga gcgtggtctc ctccacagag tttgtgaccc acgcctgctc cctcatccac
     7021 tgtgtgcact tcatcctgga ggccgttgca gtgcagcctg gagagcagct tcttagtcca
     7081 gaaagaagga caaatacccc aaaagccatc agcgaggagg aggaggaagt agatccaaac
     7141 acacagaatc ctaagtatat cactgcagcc tgtgagatgg tggcagaaat ggtggagtct
     7201 ctgcagtcgg tgttggcctt gggtcataaa aggaatagcg gcgtgccggc gtttctcacg
     7261 ccattgctca ggaacatcat catcagcctg gcccgcctgc cccttgtcaa cagctacaca
     7321 cgtgtgcccc cactggtgtg gaagcttgga tggtcaccca aaccgggagg ggattttggc
     7381 acagcattcc ctgagatccc cgtggagttc ctccaggaaa aggaagtctt taaggagttc
     7441 atctaccgca tcaacacact aggctggacc agtcgtactc agtttgaaga aacttgggcc
     7501 accctccttg gtgtcctggt gacgcagccc ctcgtgatgg agcaggagga gagcccacca
     7561 gaagaagaca cagagaggac ccagatcaac gtcctggccg tgcaggccat cacctcactg
     7621 gtgctcagtg caatgactgt gcctgtggcc ggcaacccag ctgtaagctg cttggagcag
     7681 cagccccgga acaagcctct gaaagctctc gacaccaggt ttgggaggaa gctgagcatt
     7741 atcagaggga ttgtggagca agagattcaa gcaatggttt caaagagaga gaatattgcc
     7801 acccatcatt tatatcaggc atgggatcct gtcccttctc tgtctccggc tactacaggt
     7861 gccctcatca gccacgagaa gctgctgctg cagatcaacc ccgagcggga gctggggagc
     7921 atgagctaca aactcggcca ggtgtccata cactccgtgt ggctagggaa cagcatcaca
     7981 cccctgaggg aggaggaatg ggacgaggaa gaggaggagg aggccgacgc ccctgcacct
     8041 tcgtcaccac ccacgtctcc agtcaactcc aggaaacacc gggctggagt tgacatccac
     8101 tcctgttcgc agtttttgct tgagttgtac agccgctgga tcctgccgtc cagctcagcc
     8161 aggaggaccc cggccatcct gatcagtgag gtggtcagat cccttctagt ggtctcagac
     8221 ttgttcaccg agcgcaacca gtttgagctg atgtatgtga cgctgacaga actgcgaagg
     8281 gtgcaccctt cagaagacga gatcctcgct cagtacctgg tgcctgccac ctgcaaggca
     8341 gctgccgtcc ttgggatgga caaggccgtg gcggagcctg tcagccgcct gctggagagc
     8401 acgctcagga gcagccacct gcccagcagg gttggagccc tgcacggcat cctctatgtg
     8461 ctggagtgcg acctgctgga cgacactgcc aagcagctca tcccggtcat cagcgactat
     8521 ctcctctcca acctgaaagg gatcgcccac tgcgtgaaca ttcacagcca gcagcacgta
     8581 ctggtcatgt gtgccactgc gttttacctc attgagaact atcctctgga cgtagggccg
     8641 gaattttcag catcaataat acagatgtgt ggggtgatgc tgtctggaag tgaggagtcc
     8701 accccctcca tcatttacca ctgtgccctc agaggcctgg agcgcctcct gctctctgag
     8761 cagctctccc gcctggatgc agaatcgctg gtcaagctga gtgtggacag agtgaacgtg
     8821 cacagcccgc accgggccat ggcggctctg ggcctgatgc tcacctgcat gtacacagga
     8881 aaggagaaag tcagtccggg tagaacttca gaccctaatc ctgcagcccc cgacagcgag
     8941 tcagtgattg ttgctatgga gcgggtatct gttctttttg ataggatcag gaaaggcttt
     9001 ccttgtgaag ccagagtggt ggccaggatc ctgccccagt ttctagacga cttcttccca
     9061 ccccaggaca tcatgaacaa agtcatcgga gagtttctgt ccaaccagca gccatacccc
     9121 cagttcatgg ccaccgtggt gtataaggtg tttcagactc tgcacagcac cgggcagtcg
     9181 tccatggtcc gggactgggt catgctgtcc ctctccaact tcacgcagag ggccccggtc
     9241 gccatggcca cgtggagcct ctcctgcttc tttgtcagcg cgtccaccag cccgtgggtc
     9301 gcggcgatcc tcccacatgt catcagcagg atgggcaagc tggagcaggt ggacgtgaac
     9361 cttttctgcc tggtcgccac agacttctac agacaccaga tagaggagga gctcgaccgc
     9421 agggccttcc agtctgtgct tgaggtggtt gcagccccag gaagcccata tcaccggctg
     9481 ctgacttgtt tacgaaatgt ccacaaggtc accacctgc
//
