LOCUS       sequence-209900-        9543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8861..8870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca accgccaccg
      241 ccgccgccgc cgccgccgcc tcctcagctt cctcagccgc cgccgcaggc acagccgctg
      301 ctgcctcagc cgcagccgcc cccgccgccg cccccgccgc cacccggccc ggctgtggct
      361 gaggagccgc tgcaccgacc aaagaaagaa ctttcagcta ccaagaaaga ccgtgtgaat
      421 cattgtctga caatatgtga aaacatagtg gcacagtctg tcagaaattc tccagaattt
      481 cagaaacttc tgggcatcgc tatggaactt tttctgctgt gcagtgatga cgcagagtca
      541 gatgtcagga tggtggctga cgaatgcctc aacaaagtta tcaaagcttt gatggattct
      601 aatcttccaa ggttacagct cgagctctat aaggaaatta aaaagaatgg tgcccctcgg
      661 agtttgcgtg ctgccctgtg gaggtttgct gagctggctc acctggttcg gcctcagaaa
      721 tgcaggcctt acctggtgaa ccttctgccg tgcctgactc gaacaagcaa gagacccgaa
      781 gaatcagtcc aggagacctt ggctgcagct gttcccaaaa ttatggcttc ttttggcaat
      841 tttgcaaatg acaatgaaat taaggttttg ttaaaggcct tcatagcgaa cctgaagtca
      901 agctccccca ccattcggcg gacagcggct ggatcagcag tgagcatctg ccagcactca
      961 agaaggacac aatatttcta tagttggcta ctaaatgtgc tcttaggctt actcgttcct
     1021 gtcgaggatg aacactccac tctgctgatt cttggcgtgc tgctcaccct gaggtatttg
     1081 gtgcccttgc tgcagcagca ggtcaaggac acaagcctga aaggcagctt cggagtgaca
     1141 aggaaagaaa tggaagtctc tccttctgca gagcagcttg tccaggttta tgaactgacg
     1201 ttacatcata cacagcacca agaccacaat gttgtgaccg gagccctgga gctgttgcag
     1261 cagctcttca gaacgcctcc acccgagctt ctgcaaaccc tgaccgcagt cgggggcatt
     1321 gggcagctca ccgctgctaa ggaggagtct ggtggccgaa gccgtagtgg gagtattgtg
     1381 gaacttatag ctggaggggg ttcctcatgc agccctgtcc tttcaagaaa acaaaaaggc
     1441 aaagtgctct taggagaaga agaagccttg gaggatgact ctgaatcgag atcggatgtc
     1501 agcagctctg ccttaacagc ctcagtgaag gatgagatca gtggagagct ggctgcttct
     1561 tcaggggttt ccactccagg gtcagcaggt catgacatca tcacagaaca gccacggtca
     1621 cagcacacac tgcaggcgga ctcagtggat ctggccagct gtgacttgac aagctctgcc
     1681 actgatgggg atgaggagga tatcttgagc cacagctcca gccaggtcag cgccgtccca
     1741 tctgaccctg ccatggacct gaatgatggg acccaggcct cgtcgcccat cagcgacagc
     1801 tcccagacca ccaccgaagg gcctgattca gctgttaccc cttcagacag ttctgaaatt
     1861 gtgttagacg gtaccgacaa ccagtatttg ggcctgcaga ttggacagcc ccaggatgaa
     1921 gatgaggaag ccacaggtat tcttcctgat gaagcctcgg aggccttcag gaactcttcc
     1981 atggcccttc aacaggcaca tttattgaaa aacatgagtc actgcaggca gccttctgac
     2041 agcagtgttg ataaatttgt gttgagagat gaagctactg aaccgggtga tcaagaaaac
     2101 aagccttgcc gcatcaaagg tgacattgga cagtccactg atgatgactc tgcacctctt
     2161 gtccattgtg tccgcctttt atctgcttcg tttttgctaa cagggggaaa aaatgtgctg
     2221 gttccggaca gggatgtgag ggtcagcgtg aaggccctgg ccctcagctg tgtgggagca
     2281 gctgtggccc tccacccgga atctttcttc agcaaactct ataaagttcc tcttgacacc
     2341 acggaatacc ctgaggaaca gtatgtctca gacatcttga actacatcga tcatggagac
     2401 ccacaggttc gaggagccac tgccattctc tgtgggaccc tcatctgctc catcctcagc
     2461 aggtcccgct tccacgtggg agattggatg ggcaccatta gaaccctcac aggaaataca
     2521 ttttctttgg cggattgcat tcctttgctg cggaaaacac tgaaggatga gtcttctgtt
     2581 acttgcaagt tagcttgtac agctgtgagg aactgtgtca tgagtctctg cagcagcagc
     2641 tacagtgagt taggactgca gctgatcatc gatgtgctga ctctgaggaa cagttcctat
     2701 tggctggtga ggacagagct tctggaaacc cttgcagaga ttgacttcag gctggtgagc
     2761 tttttggagg caaaagcaga aaacttacac agaggggctc atcattatac agggctttta
     2821 aaactgcaag aacgagtgct caataatgtt gtcatccatt tgcttggaga tgaagacccc
     2881 agggtgcgac atgttgccgc agcatcacta attaggcttg tcccaaagct gttttataaa
     2941 tgtgaccaag gacaagctga tccagtagtg gccgtggcaa gagatcaaag cagtgtttac
     3001 ctgaaacttc tcatgcatga gacgcagcct ccatctcatt tctccgtcag cacaataacc
     3061 agaatatata gaggctataa cctactacca agcataacag acgtcactat ggaaaataac
     3121 ctttcaagag ttattgcagc agtttctcat gaactaatca catcaaccac cagagcactc
     3181 acatttggat gctgtgaagc tttgtgtctt ctttccactg ccttcccagt ttgcatttgg
     3241 agtttaggtt ggcactgtgg agtgcctcca ctgagtgcct cagatgagtc taggaagagc
     3301 tgtaccgttg ggatggccac aatgattctg accctgctct cgtcagcttg gttcccattg
     3361 gatctctcag cccatcaaga tgctttgatt ttggccggaa acttgcttgc agccagtgct
     3421 cccaaatctc tgagaagttc atgggcctct gaagaagaag ccaacccagc agccaccaag
     3481 caagaggagg tctggccagc cctgggggac cgggccctgg tgcccatggt ggagcagctc
     3541 ttctctcacc tgctgaaggt gattaacatt tgtgcccacg tcctggatga cgtggctcct
     3601 ggacccgcaa taaaggcagc cttgccttct ctaacaaacc ccccttctct aagtcccatc
     3661 cgacgaaagg ggaaggagaa agaaccagga gaacaagcat ctgtaccgtt gagtcccaag
     3721 aaaggcagtg aggccagtgc agcttctaga caatctgata cctcaggtcc tgttacaaca
     3781 agtaaatcct catcactggg gagtttctat catcttcctt catacctcaa actgcatgat
     3841 gtcctgaaag ctacacacgc taactacaag gtcacgctgg atcttcagaa cagcacggaa
     3901 aagtttggag ggtttcttcg ctcagccttg gatgttcttt ctcagatact agagctggcc
     3961 acactgcagg acattgggaa gtgtgttgaa gagatcctag gatacctgaa atcctgcttt
     4021 agtcgagaac caatgatggc aactgtttgt gttcaacaat tgttgaagac tctctttggc
     4081 acaaacttgg cctcccagtt tgatggctta tcttccaacc ccagcaagtc acaaggccga
     4141 gcacagcgcc ttggctcctc cagtgtgagg ccaggcttgt accactactg cttcatggcc
     4201 ccgtacaccc acttcaccca ggccctcgct gacgccagcc tgaggaacat ggtgcaggcg
     4261 gagcaggaga acgacacctc gggatggttt gatgtcctcc agaaagtgtc tacccagttg
     4321 aagacaaacc tcacgagtgt cacaaagaac cgtgcagata agaatgctat tcataatcac
     4381 attcgtttgt ttgaacctct tgttataaaa gctttaaaac agtacacgac tacaacatgt
     4441 gtgcagttac agaagcaggt tttagatttg ctggcgcagc tggttcagtt acgggttaat
     4501 tactgtcttc tggattcaga tcaggtgttt attggctttg tattgaaaca gtttgaatac
     4561 attgaagtgg gccagttcag ggaatcagag gcaatcattc caaacatctt tttcttcttg
     4621 gtattactat cttatgaacg ctatcattca aaacagatca ttggaattcc taaaatcatt
     4681 cagctctgtg atggcatcat ggccagtgga aggaaggctg tgacacatgc cataccggct
     4741 ctgcagccca tagtccacga cctctttgta ttaagaggaa caaataaagc tgatgcagga
     4801 aaagagcttg aaacccaaaa agaggtggtg gtgtcaatgt tactgagact catccagtac
     4861 catcaggtgt tggagatgtt cattcttgtc ctgcagcagt gccacaagga gaatgaagac
     4921 aagtggaagc gactgtctcg acagatagct gacatcatcc tcccaatgtt agccaaacag
     4981 cagatgcaca ttgactctca tgaagccctt ggagtgttaa atacattatt tgagattttg
     5041 gccccttcct ccctccgtcc ggtagacatg cttttacgga gtatgttcgt cactccaaac
     5101 acaatggcgt ccgtgagcac tgttcaactg tggatatcgg gaattctggc cattttgagg
     5161 gttctgattt cccagtcaac tgaagatatt gttctttctc gtattcagga gctctccttc
     5221 tctccgtatt taatctcctg tacagtaatt aataggttaa gagatgggga cagtacttca
     5281 acgctagaag aacacagtga agggaaacaa ataaagaatt tgccagaaga aacattttca
     5341 aggtttctat tacaactggt tggtattctt ttagaagaca ttgttacaaa acagctgaag
     5401 gtggaaatga gtgagcagca acatactttc tattgccagg aactaggcac actgctaatg
     5461 tgtctgatcc acatcttcaa gtctggaatg ttccggagaa tcacagcagc tgccactagg
     5521 ctgttccgca gtgatggctg tggcggcagt ttctacaccc tggacagctt gaacttgcgg
     5581 gctcgttcca tgatcaccac ccacccggcc ctggtgctgc tctggtgtca gatactgctg
     5641 cttgtcaacc acaccgacta ccgctggtgg gcagaagtgc agcagacccc gaaaagacac
     5701 agtctgtcca gcacaaagtt acttagtccc cagatgtctg gagaagagga ggattctgac
     5761 ttggcagcca aacttggaat gtgcaataga gaaatagtac gaagaggggc tctcattctc
     5821 ttctgtgatt atgtctgtca gaacctccat gactccgagc acttaacgtg gctcattgta
     5881 aatcacattc aagatctgat cagcctttcc cacgagcctc cagtacagga cttcatcagt
     5941 gccgttcatc ggaactctgc tgccagcggc ctgttcatcc aggcaattca gtctcgttgt
     6001 gaaaaccttt caactccaac catgctgaag aaaactcttc agtgcttgga ggggatccat
     6061 ctcagccagt cgggagctgt gctcacgctg tatgtggaca ggcttctgtg cacccctttc
     6121 cgtgtgctgg ctcgcatggt cgacatcctt gcttgtcgcc gggtagaaat gcttctggct
     6181 gcaaatttac agagcagcat ggcccagttg ccaatggaag aactcaacag aatccaggaa
     6241 taccttcaga gcagcgggct cgctcagaga caccaaaggc tctattccct gctggacagg
     6301 tttcgtctct ccaccatgca agactcactt agtccctctc ctccagtctc ttcccacccg
     6361 ctggacgggg atgggcacgt gtcactggaa acagtgagtc cggacaaaga ctggtacgtt
     6421 catcttgtca aatcccagtg ttggaccagg tcagattctg cactgctgga aggtgcagag
     6481 ctggtgaatc ggattcctgc tgaagatatg aatgccttca tgatgaactc ggagttcaac
     6541 ctaagcctgc tagctccatg cttaagccta gggatgagtg aaatttctgg tggccagaag
     6601 agtgcccttt ttgaagcagc ccgtgaggtg actctggccc gtgtgagcgg caccgtgcag
     6661 cagctccctg ctgtccatca tgtcttccag cccgagctgc ctgcagaacc ggcggcctac
     6721 tggagcaagt tgaatgatct gtttggggat gctgcactgt atcagtccct gcccactctg
     6781 gcccgggccc tggcacagta cctggtggtg gtctccaaac tgcccagtca tttgcacctt
     6841 cctcctgaga aagagaagga cattgtgaaa ttcgtggtgg caacccttga ggccctgtcc
     6901 tggcatttga tccatgagca gatcccgctg agtctggatc tccaggcagg gctggactgc
     6961 tgctgcctgg ccctgcagct gcctggcctc tggagcgtgg tctcctccac agagtttgtg
     7021 acccacgcct gctccctcat ccactgtgtg cacttcatcc tggaggccgt tgcagtgcag
     7081 cctggagagc agcttcttag tccagaaaga aggacaaata ccccaaaagc catcagcgag
     7141 gaggaggagg aagtagatcc aaacacacag aatcctaagt atatcactgc agcctgtgag
     7201 atggtggcag aaatggtgga gtctctgcag tcggtgttgg ccttgggtca taaaaggaat
     7261 agcggcgtgc cggcgtttct cacgccattg ctcaggaaca tcatcatcag cctggcccgc
     7321 ctgccccttg tcaacagcta cacacgtgtg cccccactgg tgtggaagct tggatggtca
     7381 cccaaaccgg gaggggattt tggcacagca ttccctgaga tccccgtgga gttcctccag
     7441 gaaaaggaag tctttaagga gttcatctac cgcatcaaca cactaggctg gaccagtcgt
     7501 actcagtttg aagaaacttg ggccaccctc cttggtgtcc tggtgacgca gcccctcgtg
     7561 atggagcagg aggagagccc accagaagaa gacacagaga ggacccagat caacgtcctg
     7621 gccgtgcagg ccatcacctc actggtgctc agtgcaatga ctgtgcctgt ggccggcaac
     7681 ccagctgtaa gctgcttgga gcagcagccc cggaacaagc ctctgaaagc tctcgacacc
     7741 aggtttggga ggaagctgag cattatcaga gggattgtgg agcaagagat tcaagcaatg
     7801 gtttcaaaga gagagaatat tgccacccat catttatatc aggcatggga tcctgtccct
     7861 tctctgtctc cggctactac aggtgccctc atcagccacg agaagctgct gctgcagatc
     7921 aaccccgagc gggagctggg gagcatgagc tacaaactcg gccaggtgtc catacactcc
     7981 gtgtggctag ggaacagcat cacacccctg agggaggagg aatgggacga ggaagaggag
     8041 gaggaggccg acgcccctgc accttcgtca ccacccacgt ctccagtcaa ctccaggaaa
     8101 caccgggctg gagttgacat ccactcctgt tcgcagtttt tgcttgagtt gtacagccgc
     8161 tggatcctgc cgtccagctc agccaggagg accccggcca tcctgatcag tgaggtggtc
     8221 agatcccttc tagtggtctc agacttgttc accgagcgca accagtttga gctgatgtat
     8281 gtgacgctga cagaactgcg aagggtgcac ccttcagaag acgagatcct cgctcagtac
     8341 ctggtgcctg ccacctgcaa ggcagctgcc gtccttggga tggacaaggc cgtggcggag
     8401 cctgtcagcc gcctgctgga gagcacgctc aggagcagcc acctgcccag cagggttgga
     8461 gccctgcacg gcatcctcta tgtgctggag tgcgacctgc tggacgacac tgccaagcag
     8521 ctcatcccgg tcatcagcga ctatctcctc tccaacctga aagggatcgc ccactgcgtg
     8581 aacattcaca gccagcagca cgtactggtc atgtgtgcca ctgcgtttta cctcattgag
     8641 aactatcctc tggacgtagg gccggaattt tcagcatcaa taatacagat gtgtggggtg
     8701 atgctgtctg gaagtgagga gtccaccccc tccatcattt accactgtgc cctcagaggc
     8761 ctggagcgcc tcctgctctc tgagcagctc tcccgcctgg atgcagaatc gctggtcaag
     8821 ctgagtgtgg acagagtgaa cgtgcacagc ccgcaccggg ccatggcggc tctgggcctg
     8881 atgctcacct gcatgtacac aggaaaggag aaagtcagtc cgggtagaac ttcagaccct
     8941 aatcctgcag cccccgacag cgagtcagtg attgttgcta tggagcgggt atctgttctt
     9001 tttgatagga tcaggaaagg ctttccttgt gaagccagag tggtggccag gatcctgccc
     9061 cagtttctag acgacttctt cccaccccag gacatcatga acaaagtcat cggagagttt
     9121 ctgtccaacc agcagccata cccccagttc atggccaccg tggtgtataa ggtgtttcag
     9181 actctgcaca gcaccgggca gtcgtccatg gtccgggact gggtcatgct gtccctctcc
     9241 aacttcacgc agagggcccc ggtcgccatg gccacgtgga gcctctcctg cttctttgtc
     9301 agcgcgtcca ccagcccgtg ggtcgcggcg atcctcccac atgtcatcag caggatgggc
     9361 aagctggagc aggtggacgt gaaccttttc tgcctggtcg ccacagactt ctacagacac
     9421 cagatagagg aggagctcga ccgcagggcc ttccagtctg tgcttgaggt ggttgcagcc
     9481 ccaggaagcc catatcaccg gctgctgact tgtttacgaa atgtccacaa ggtcaccacc
     9541 tgc
//
