LOCUS       sequence-209901-        9561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8879..8888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa
      181 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      241 caacagcaac cgccaccgcc gccgccgccg ccgccgcctc ctcagcttcc tcagccgccg
      301 ccgcaggcac agccgctgct gcctcagccg cagccgcccc cgccgccgcc cccgccgcca
      361 cccggcccgg ctgtggctga ggagccgctg caccgaccaa agaaagaact ttcagctacc
      421 aagaaagacc gtgtgaatca ttgtctgaca atatgtgaaa acatagtggc acagtctgtc
      481 agaaattctc cagaatttca gaaacttctg ggcatcgcta tggaactttt tctgctgtgc
      541 agtgatgacg cagagtcaga tgtcaggatg gtggctgacg aatgcctcaa caaagttatc
      601 aaagctttga tggattctaa tcttccaagg ttacagctcg agctctataa ggaaattaaa
      661 aagaatggtg cccctcggag tttgcgtgct gccctgtgga ggtttgctga gctggctcac
      721 ctggttcggc ctcagaaatg caggccttac ctggtgaacc ttctgccgtg cctgactcga
      781 acaagcaaga gacccgaaga atcagtccag gagaccttgg ctgcagctgt tcccaaaatt
      841 atggcttctt ttggcaattt tgcaaatgac aatgaaatta aggttttgtt aaaggccttc
      901 atagcgaacc tgaagtcaag ctcccccacc attcggcgga cagcggctgg atcagcagtg
      961 agcatctgcc agcactcaag aaggacacaa tatttctata gttggctact aaatgtgctc
     1021 ttaggcttac tcgttcctgt cgaggatgaa cactccactc tgctgattct tggcgtgctg
     1081 ctcaccctga ggtatttggt gcccttgctg cagcagcagg tcaaggacac aagcctgaaa
     1141 ggcagcttcg gagtgacaag gaaagaaatg gaagtctctc cttctgcaga gcagcttgtc
     1201 caggtttatg aactgacgtt acatcataca cagcaccaag accacaatgt tgtgaccgga
     1261 gccctggagc tgttgcagca gctcttcaga acgcctccac ccgagcttct gcaaaccctg
     1321 accgcagtcg ggggcattgg gcagctcacc gctgctaagg aggagtctgg tggccgaagc
     1381 cgtagtggga gtattgtgga acttatagct ggagggggtt cctcatgcag ccctgtcctt
     1441 tcaagaaaac aaaaaggcaa agtgctctta ggagaagaag aagccttgga ggatgactct
     1501 gaatcgagat cggatgtcag cagctctgcc ttaacagcct cagtgaagga tgagatcagt
     1561 ggagagctgg ctgcttcttc aggggtttcc actccagggt cagcaggtca tgacatcatc
     1621 acagaacagc cacggtcaca gcacacactg caggcggact cagtggatct ggccagctgt
     1681 gacttgacaa gctctgccac tgatggggat gaggaggata tcttgagcca cagctccagc
     1741 caggtcagcg ccgtcccatc tgaccctgcc atggacctga atgatgggac ccaggcctcg
     1801 tcgcccatca gcgacagctc ccagaccacc accgaagggc ctgattcagc tgttacccct
     1861 tcagacagtt ctgaaattgt gttagacggt accgacaacc agtatttggg cctgcagatt
     1921 ggacagcccc aggatgaaga tgaggaagcc acaggtattc ttcctgatga agcctcggag
     1981 gccttcagga actcttccat ggcccttcaa caggcacatt tattgaaaaa catgagtcac
     2041 tgcaggcagc cttctgacag cagtgttgat aaatttgtgt tgagagatga agctactgaa
     2101 ccgggtgatc aagaaaacaa gccttgccgc atcaaaggtg acattggaca gtccactgat
     2161 gatgactctg cacctcttgt ccattgtgtc cgccttttat ctgcttcgtt tttgctaaca
     2221 gggggaaaaa atgtgctggt tccggacagg gatgtgaggg tcagcgtgaa ggccctggcc
     2281 ctcagctgtg tgggagcagc tgtggccctc cacccggaat ctttcttcag caaactctat
     2341 aaagttcctc ttgacaccac ggaataccct gaggaacagt atgtctcaga catcttgaac
     2401 tacatcgatc atggagaccc acaggttcga ggagccactg ccattctctg tgggaccctc
     2461 atctgctcca tcctcagcag gtcccgcttc cacgtgggag attggatggg caccattaga
     2521 accctcacag gaaatacatt ttctttggcg gattgcattc ctttgctgcg gaaaacactg
     2581 aaggatgagt cttctgttac ttgcaagtta gcttgtacag ctgtgaggaa ctgtgtcatg
     2641 agtctctgca gcagcagcta cagtgagtta ggactgcagc tgatcatcga tgtgctgact
     2701 ctgaggaaca gttcctattg gctggtgagg acagagcttc tggaaaccct tgcagagatt
     2761 gacttcaggc tggtgagctt tttggaggca aaagcagaaa acttacacag aggggctcat
     2821 cattatacag ggcttttaaa actgcaagaa cgagtgctca ataatgttgt catccatttg
     2881 cttggagatg aagaccccag ggtgcgacat gttgccgcag catcactaat taggcttgtc
     2941 ccaaagctgt tttataaatg tgaccaagga caagctgatc cagtagtggc cgtggcaaga
     3001 gatcaaagca gtgtttacct gaaacttctc atgcatgaga cgcagcctcc atctcatttc
     3061 tccgtcagca caataaccag aatatataga ggctataacc tactaccaag cataacagac
     3121 gtcactatgg aaaataacct ttcaagagtt attgcagcag tttctcatga actaatcaca
     3181 tcaaccacca gagcactcac atttggatgc tgtgaagctt tgtgtcttct ttccactgcc
     3241 ttcccagttt gcatttggag tttaggttgg cactgtggag tgcctccact gagtgcctca
     3301 gatgagtcta ggaagagctg taccgttggg atggccacaa tgattctgac cctgctctcg
     3361 tcagcttggt tcccattgga tctctcagcc catcaagatg ctttgatttt ggccggaaac
     3421 ttgcttgcag ccagtgctcc caaatctctg agaagttcat gggcctctga agaagaagcc
     3481 aacccagcag ccaccaagca agaggaggtc tggccagccc tgggggaccg ggccctggtg
     3541 cccatggtgg agcagctctt ctctcacctg ctgaaggtga ttaacatttg tgcccacgtc
     3601 ctggatgacg tggctcctgg acccgcaata aaggcagcct tgccttctct aacaaacccc
     3661 ccttctctaa gtcccatccg acgaaagggg aaggagaaag aaccaggaga acaagcatct
     3721 gtaccgttga gtcccaagaa aggcagtgag gccagtgcag cttctagaca atctgatacc
     3781 tcaggtcctg ttacaacaag taaatcctca tcactgggga gtttctatca tcttccttca
     3841 tacctcaaac tgcatgatgt cctgaaagct acacacgcta actacaaggt cacgctggat
     3901 cttcagaaca gcacggaaaa gtttggaggg tttcttcgct cagccttgga tgttctttct
     3961 cagatactag agctggccac actgcaggac attgggaagt gtgttgaaga gatcctagga
     4021 tacctgaaat cctgctttag tcgagaacca atgatggcaa ctgtttgtgt tcaacaattg
     4081 ttgaagactc tctttggcac aaacttggcc tcccagtttg atggcttatc ttccaacccc
     4141 agcaagtcac aaggccgagc acagcgcctt ggctcctcca gtgtgaggcc aggcttgtac
     4201 cactactgct tcatggcccc gtacacccac ttcacccagg ccctcgctga cgccagcctg
     4261 aggaacatgg tgcaggcgga gcaggagaac gacacctcgg gatggtttga tgtcctccag
     4321 aaagtgtcta cccagttgaa gacaaacctc acgagtgtca caaagaaccg tgcagataag
     4381 aatgctattc ataatcacat tcgtttgttt gaacctcttg ttataaaagc tttaaaacag
     4441 tacacgacta caacatgtgt gcagttacag aagcaggttt tagatttgct ggcgcagctg
     4501 gttcagttac gggttaatta ctgtcttctg gattcagatc aggtgtttat tggctttgta
     4561 ttgaaacagt ttgaatacat tgaagtgggc cagttcaggg aatcagaggc aatcattcca
     4621 aacatctttt tcttcttggt attactatct tatgaacgct atcattcaaa acagatcatt
     4681 ggaattccta aaatcattca gctctgtgat ggcatcatgg ccagtggaag gaaggctgtg
     4741 acacatgcca taccggctct gcagcccata gtccacgacc tctttgtatt aagaggaaca
     4801 aataaagctg atgcaggaaa agagcttgaa acccaaaaag aggtggtggt gtcaatgtta
     4861 ctgagactca tccagtacca tcaggtgttg gagatgttca ttcttgtcct gcagcagtgc
     4921 cacaaggaga atgaagacaa gtggaagcga ctgtctcgac agatagctga catcatcctc
     4981 ccaatgttag ccaaacagca gatgcacatt gactctcatg aagcccttgg agtgttaaat
     5041 acattatttg agattttggc cccttcctcc ctccgtccgg tagacatgct tttacggagt
     5101 atgttcgtca ctccaaacac aatggcgtcc gtgagcactg ttcaactgtg gatatcggga
     5161 attctggcca ttttgagggt tctgatttcc cagtcaactg aagatattgt tctttctcgt
     5221 attcaggagc tctccttctc tccgtattta atctcctgta cagtaattaa taggttaaga
     5281 gatggggaca gtacttcaac gctagaagaa cacagtgaag ggaaacaaat aaagaatttg
     5341 ccagaagaaa cattttcaag gtttctatta caactggttg gtattctttt agaagacatt
     5401 gttacaaaac agctgaaggt ggaaatgagt gagcagcaac atactttcta ttgccaggaa
     5461 ctaggcacac tgctaatgtg tctgatccac atcttcaagt ctggaatgtt ccggagaatc
     5521 acagcagctg ccactaggct gttccgcagt gatggctgtg gcggcagttt ctacaccctg
     5581 gacagcttga acttgcgggc tcgttccatg atcaccaccc acccggccct ggtgctgctc
     5641 tggtgtcaga tactgctgct tgtcaaccac accgactacc gctggtgggc agaagtgcag
     5701 cagaccccga aaagacacag tctgtccagc acaaagttac ttagtcccca gatgtctgga
     5761 gaagaggagg attctgactt ggcagccaaa cttggaatgt gcaatagaga aatagtacga
     5821 agaggggctc tcattctctt ctgtgattat gtctgtcaga acctccatga ctccgagcac
     5881 ttaacgtggc tcattgtaaa tcacattcaa gatctgatca gcctttccca cgagcctcca
     5941 gtacaggact tcatcagtgc cgttcatcgg aactctgctg ccagcggcct gttcatccag
     6001 gcaattcagt ctcgttgtga aaacctttca actccaacca tgctgaagaa aactcttcag
     6061 tgcttggagg ggatccatct cagccagtcg ggagctgtgc tcacgctgta tgtggacagg
     6121 cttctgtgca cccctttccg tgtgctggct cgcatggtcg acatccttgc ttgtcgccgg
     6181 gtagaaatgc ttctggctgc aaatttacag agcagcatgg cccagttgcc aatggaagaa
     6241 ctcaacagaa tccaggaata ccttcagagc agcgggctcg ctcagagaca ccaaaggctc
     6301 tattccctgc tggacaggtt tcgtctctcc accatgcaag actcacttag tccctctcct
     6361 ccagtctctt cccacccgct ggacggggat gggcacgtgt cactggaaac agtgagtccg
     6421 gacaaagact ggtacgttca tcttgtcaaa tcccagtgtt ggaccaggtc agattctgca
     6481 ctgctggaag gtgcagagct ggtgaatcgg attcctgctg aagatatgaa tgccttcatg
     6541 atgaactcgg agttcaacct aagcctgcta gctccatgct taagcctagg gatgagtgaa
     6601 atttctggtg gccagaagag tgcccttttt gaagcagccc gtgaggtgac tctggcccgt
     6661 gtgagcggca ccgtgcagca gctccctgct gtccatcatg tcttccagcc cgagctgcct
     6721 gcagaaccgg cggcctactg gagcaagttg aatgatctgt ttggggatgc tgcactgtat
     6781 cagtccctgc ccactctggc ccgggccctg gcacagtacc tggtggtggt ctccaaactg
     6841 cccagtcatt tgcaccttcc tcctgagaaa gagaaggaca ttgtgaaatt cgtggtggca
     6901 acccttgagg ccctgtcctg gcatttgatc catgagcaga tcccgctgag tctggatctc
     6961 caggcagggc tggactgctg ctgcctggcc ctgcagctgc ctggcctctg gagcgtggtc
     7021 tcctccacag agtttgtgac ccacgcctgc tccctcatcc actgtgtgca cttcatcctg
     7081 gaggccgttg cagtgcagcc tggagagcag cttcttagtc cagaaagaag gacaaatacc
     7141 ccaaaagcca tcagcgagga ggaggaggaa gtagatccaa acacacagaa tcctaagtat
     7201 atcactgcag cctgtgagat ggtggcagaa atggtggagt ctctgcagtc ggtgttggcc
     7261 ttgggtcata aaaggaatag cggcgtgccg gcgtttctca cgccattgct caggaacatc
     7321 atcatcagcc tggcccgcct gccccttgtc aacagctaca cacgtgtgcc cccactggtg
     7381 tggaagcttg gatggtcacc caaaccggga ggggattttg gcacagcatt ccctgagatc
     7441 cccgtggagt tcctccagga aaaggaagtc tttaaggagt tcatctaccg catcaacaca
     7501 ctaggctgga ccagtcgtac tcagtttgaa gaaacttggg ccaccctcct tggtgtcctg
     7561 gtgacgcagc ccctcgtgat ggagcaggag gagagcccac cagaagaaga cacagagagg
     7621 acccagatca acgtcctggc cgtgcaggcc atcacctcac tggtgctcag tgcaatgact
     7681 gtgcctgtgg ccggcaaccc agctgtaagc tgcttggagc agcagccccg gaacaagcct
     7741 ctgaaagctc tcgacaccag gtttgggagg aagctgagca ttatcagagg gattgtggag
     7801 caagagattc aagcaatggt ttcaaagaga gagaatattg ccacccatca tttatatcag
     7861 gcatgggatc ctgtcccttc tctgtctccg gctactacag gtgccctcat cagccacgag
     7921 aagctgctgc tgcagatcaa ccccgagcgg gagctgggga gcatgagcta caaactcggc
     7981 caggtgtcca tacactccgt gtggctaggg aacagcatca cacccctgag ggaggaggaa
     8041 tgggacgagg aagaggagga ggaggccgac gcccctgcac cttcgtcacc acccacgtct
     8101 ccagtcaact ccaggaaaca ccgggctgga gttgacatcc actcctgttc gcagtttttg
     8161 cttgagttgt acagccgctg gatcctgccg tccagctcag ccaggaggac cccggccatc
     8221 ctgatcagtg aggtggtcag atcccttcta gtggtctcag acttgttcac cgagcgcaac
     8281 cagtttgagc tgatgtatgt gacgctgaca gaactgcgaa gggtgcaccc ttcagaagac
     8341 gagatcctcg ctcagtacct ggtgcctgcc acctgcaagg cagctgccgt ccttgggatg
     8401 gacaaggccg tggcggagcc tgtcagccgc ctgctggaga gcacgctcag gagcagccac
     8461 ctgcccagca gggttggagc cctgcacggc atcctctatg tgctggagtg cgacctgctg
     8521 gacgacactg ccaagcagct catcccggtc atcagcgact atctcctctc caacctgaaa
     8581 gggatcgccc actgcgtgaa cattcacagc cagcagcacg tactggtcat gtgtgccact
     8641 gcgttttacc tcattgagaa ctatcctctg gacgtagggc cggaattttc agcatcaata
     8701 atacagatgt gtggggtgat gctgtctgga agtgaggagt ccaccccctc catcatttac
     8761 cactgtgccc tcagaggcct ggagcgcctc ctgctctctg agcagctctc ccgcctggat
     8821 gcagaatcgc tggtcaagct gagtgtggac agagtgaacg tgcacagccc gcaccgggcc
     8881 atggcggctc tgggcctgat gctcacctgc atgtacacag gaaaggagaa agtcagtccg
     8941 ggtagaactt cagaccctaa tcctgcagcc cccgacagcg agtcagtgat tgttgctatg
     9001 gagcgggtat ctgttctttt tgataggatc aggaaaggct ttccttgtga agccagagtg
     9061 gtggccagga tcctgcccca gtttctagac gacttcttcc caccccagga catcatgaac
     9121 aaagtcatcg gagagtttct gtccaaccag cagccatacc cccagttcat ggccaccgtg
     9181 gtgtataagg tgtttcagac tctgcacagc accgggcagt cgtccatggt ccgggactgg
     9241 gtcatgctgt ccctctccaa cttcacgcag agggccccgg tcgccatggc cacgtggagc
     9301 ctctcctgct tctttgtcag cgcgtccacc agcccgtggg tcgcggcgat cctcccacat
     9361 gtcatcagca ggatgggcaa gctggagcag gtggacgtga accttttctg cctggtcgcc
     9421 acagacttct acagacacca gatagaggag gagctcgacc gcagggcctt ccagtctgtg
     9481 cttgaggtgg ttgcagcccc aggaagccca tatcaccggc tgctgacttg tttacgaaat
     9541 gtccacaagg tcaccacctg c
//
