LOCUS       sequence-209902-       15902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intermediate vector containing HTT gene, except for polyQ region. 
            Used to clone various polyQ lengths into HTT sequence with N-term 
            FLAG tag. Baculovirus transfer vector for insect and mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15902)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N entry vector
FEATURES             Location/Qualifiers
     source          1..15902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     399..420
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        584..688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(689..757,758..1549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(907..926)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1720..2308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2209..2228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2462..2479
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4161..4209
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4234..4253
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4315..4694
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4695..4898
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4849..4869
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4945..5054
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             5089..5112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      13654..13663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14358..14387
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14388..14411
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14502..14522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14920..14931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15156..15290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15193..15212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15247..15266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(15300..15332)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(15550..15641)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(15608..15625)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     mobile_element  15655..15820
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagca gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtaccg catgctggat ctgtacgggc cagatatacg cgttgacatt
     4321 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4381 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     4441 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     4501 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
     4561 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     4621 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     4681 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     4741 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     4801 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4861 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
     4921 ctgcttactg gcttatcgaa tacggacctt taattcaacc caacacaata tattatagtt
     4981 aaataagaat tattatcaaa tcatttgtat attaattaaa atactatact gtaaattaca
     5041 ttttatttac aatcactcga cggatcctta agaaggagat atactatgga ctacaaagac
     5101 gatgacgaca agatggcgac cctggaaaag cgctgacctt agtcgctaac ctgcaggagc
     5161 cgctgcaccg accaaagaaa gaactttcag ctaccaagaa agaccgtgtg aatcattgtc
     5221 tgacaatatg tgaaaacata gtggcacagt ctgtcagaaa ttctccagaa tttcagaaac
     5281 ttctgggcat cgctatggaa ctttttctgc tgtgcagtga tgacgcagag tcagatgtca
     5341 ggatggtggc tgacgaatgc ctcaacaaag ttatcaaagc tttgatggat tctaatcttc
     5401 caaggttaca gctcgagctc tataaggaaa ttaaaaagaa tggtgcccct cggagtttgc
     5461 gtgctgccct gtggaggttt gctgagctgg ctcacctggt tcggcctcag aaatgcaggc
     5521 cttacctggt gaaccttctg ccgtgcctga ctcgaacaag caagagaccc gaagaatcag
     5581 tccaggagac cttggctgca gctgttccca aaattatggc ttcttttggc aattttgcaa
     5641 atgacaatga aattaaggtt ttgttaaagg ccttcatagc gaacctgaag tcaagctccc
     5701 ccaccattcg gcggacagcg gctggatcag cagtgagcat ctgccagcac tcaagaagga
     5761 cacaatattt ctatagttgg ctactaaatg tgctcttagg cttactcgtt cctgtcgagg
     5821 atgaacactc cactctgctg attcttggcg tgctgctcac cctgaggtat ttggtgccct
     5881 tgctgcagca gcaggtcaag gacacaagcc tgaaaggcag cttcggagtg acaaggaaag
     5941 aaatggaagt ctctccttct gcagagcagc ttgtccaggt ttatgaactg acgttacatc
     6001 atacacagca ccaagaccac aatgttgtga ccggagccct ggagctgttg cagcagctct
     6061 tcagaacgcc tccacccgag cttctgcaaa ccctgaccgc agtcgggggc attgggcagc
     6121 tcaccgctgc taaggaggag tctggtggcc gaagccgtag tgggagtatt gtggaactta
     6181 tagctggagg gggttcctca tgcagccctg tcctttcaag aaaacaaaaa ggcaaagtgc
     6241 tcttaggaga agaagaagcc ttggaggatg actctgaatc gagatcggat gtcagcagct
     6301 ctgccttaac agcctcagtg aaggatgaga tcagtggaga gctggctgct tcttcagggg
     6361 tttccactcc agggtcagca ggtcatgaca tcatcacaga acagccacgg tcacagcaca
     6421 cactgcaggc ggactcagtg gatctggcca gctgtgactt gacaagctct gccactgatg
     6481 gggatgagga ggatatcttg agccacagct ccagccaggt cagcgccgtc ccatctgacc
     6541 ctgccatgga cctgaatgat gggacccagg cctcgtcgcc catcagcgac agctcccaga
     6601 ccaccaccga agggcctgat tcagctgtta ccccttcaga cagttctgaa attgtgttag
     6661 acggtaccga caaccagtat ttgggcctgc agattggaca gccccaggat gaagatgagg
     6721 aagccacagg tattcttcct gatgaagcct cggaggcctt caggaactct tccatggccc
     6781 ttcaacaggc acatttattg aaaaacatga gtcactgcag gcagccttct gacagcagtg
     6841 ttgataaatt tgtgttgaga gatgaagcta ctgaaccggg tgatcaagaa aacaagcctt
     6901 gccgcatcaa aggtgacatt ggacagtcca ctgatgatga ctctgcacct cttgtccatt
     6961 gtgtccgcct tttatctgct tcgtttttgc taacaggggg aaaaaatgtg ctggttccgg
     7021 acagggatgt gagggtcagc gtgaaggccc tggccctcag ctgtgtggga gcagctgtgg
     7081 ccctccaccc ggaatctttc ttcagcaaac tctataaagt tcctcttgac accacggaat
     7141 accctgagga acagtatgtc tcagacatct tgaactacat cgatcatgga gacccacagg
     7201 ttcgaggagc cactgccatt ctctgtggga ccctcatctg ctccatcctc agcaggtccc
     7261 gcttccacgt gggagattgg atgggcacca ttagaaccct cacaggaaat acattttctt
     7321 tggcggattg cattcctttg ctgcggaaaa cactgaagga tgagtcttct gttacttgca
     7381 agttagcttg tacagctgtg aggaactgtg tcatgagtct ctgcagcagc agctacagtg
     7441 agttaggact gcagctgatc atcgatgtgc tgactctgag gaacagttcc tattggctgg
     7501 tgaggacaga gcttctggaa acccttgcag agattgactt caggctggtg agctttttgg
     7561 aggcaaaagc agaaaactta cacagagggg ctcatcatta tacagggctt ttaaaactgc
     7621 aagaacgagt gctcaataat gttgtcatcc atttgcttgg agatgaagac cccagggtgc
     7681 gacatgttgc cgcagcatca ctaattaggc ttgtcccaaa gctgttttat aaatgtgacc
     7741 aaggacaagc tgatccagta gtggccgtgg caagagatca aagcagtgtt tacctgaaac
     7801 ttctcatgca tgagacgcag cctccatctc atttctccgt cagcacaata accagaatat
     7861 atagaggcta taacctacta ccaagcataa cagacgtcac tatggaaaat aacctttcaa
     7921 gagttattgc agcagtttct catgaactaa tcacatcaac caccagagca ctcacatttg
     7981 gatgctgtga agctttgtgt cttctttcca ctgccttccc agtttgcatt tggagtttag
     8041 gttggcactg tggagtgcct ccactgagtg cctcagatga gtctaggaag agctgtaccg
     8101 ttgggatggc cacaatgatt ctgaccctgc tctcgtcagc ttggttccca ttggatctct
     8161 cagcccatca agatgctttg attttggccg gaaacttgct tgcagccagt gctcccaaat
     8221 ctctgagaag ttcatgggcc tctgaagaag aagccaaccc agcagccacc aagcaagagg
     8281 aggtctggcc agccctgggg gaccgggccc tggtgcccat ggtggagcag ctcttctctc
     8341 acctgctgaa ggtgattaac atttgtgccc acgtcctgga tgacgtggct cctggacccg
     8401 caataaaggc agccttgcct tctctaacaa accccccttc tctaagtccc atccgacgaa
     8461 aggggaagga gaaagaacca ggagaacaag catctgtacc gttgagtccc aagaaaggca
     8521 gtgaggccag tgcagcttct agacaatctg atacctcagg tcctgttaca acaagtaaat
     8581 cctcatcact ggggagtttc tatcatcttc cttcatacct caaactgcat gatgtcctga
     8641 aagctacaca cgctaactac aaggtcacgc tggatcttca gaacagcacg gaaaagtttg
     8701 gagggtttct tcgctcagcc ttggatgttc tttctcagat actagagctg gccacactgc
     8761 aggacattgg gaagtgtgtt gaagagatcc taggatacct gaaatcctgc tttagtcgag
     8821 aaccaatgat ggcaactgtt tgtgttcaac aattgttgaa gactctcttt ggcacaaact
     8881 tggcctccca gtttgatggc ttatcttcca accccagcaa gtcacaaggc cgagcacagc
     8941 gccttggctc ctccagtgtg aggccaggct tgtaccacta ctgcttcatg gccccgtaca
     9001 cccacttcac ccaggccctc gctgacgcca gcctgaggaa catggtgcag gcggagcagg
     9061 agaacgacac ctcgggatgg tttgatgtcc tccagaaagt gtctacccag ttgaagacaa
     9121 acctcacgag tgtcacaaag aaccgtgcag ataagaatgc tattcataat cacattcgtt
     9181 tgtttgaacc tcttgttata aaagctttaa aacagtacac gactacaaca tgtgtgcagt
     9241 tacagaagca ggttttagat ttgctggcgc agctggttca gttacgggtt aattactgtc
     9301 ttctggattc agatcaggtg tttattggct ttgtattgaa acagtttgaa tacattgaag
     9361 tgggccagtt cagggaatca gaggcaatca ttccaaacat ctttttcttc ttggtattac
     9421 tatcttatga acgctatcat tcaaaacaga tcattggaat tcctaaaatc attcagctct
     9481 gtgatggcat catggccagt ggaaggaagg ctgtgacaca tgccataccg gctctgcagc
     9541 ccatagtcca cgacctcttt gtattaagag gaacaaataa agctgatgca ggaaaagagc
     9601 ttgaaaccca aaaagaggtg gtggtgtcaa tgttactgag actcatccag taccatcagg
     9661 tgttggagat gttcattctt gtcctgcagc agtgccacaa ggagaatgaa gacaagtgga
     9721 agcgactgtc tcgacagata gctgacatca tcctcccaat gttagccaaa cagcagatgc
     9781 acattgactc tcatgaagcc cttggagtgt taaatacatt atttgagatt ttggcccctt
     9841 cctccctccg tccggtagac atgcttttac ggagtatgtt cgtcactcca aacacaatgg
     9901 cgtccgtgag cactgttcaa ctgtggatat cgggaattct ggccattttg agggttctga
     9961 tttcccagtc aactgaagat attgttcttt ctcgtattca ggagctctcc ttctctccgt
    10021 atttaatctc ctgtacagta attaataggt taagagatgg ggacagtact tcaacgctag
    10081 aagaacacag tgaagggaaa caaataaaga atttgccaga agaaacattt tcaaggtttc
    10141 tattacaact ggttggtatt cttttagaag acattgttac aaaacagctg aaggtggaaa
    10201 tgagtgagca gcaacatact ttctattgcc aggaactagg cacactgcta atgtgtctga
    10261 tccacatctt caagtctgga atgttccgga gaatcacagc agctgccact aggctgttcc
    10321 gcagtgatgg ctgtggcggc agtttctaca ccctggacag cttgaacttg cgggctcgtt
    10381 ccatgatcac cacccacccg gccctggtgc tgctctggtg tcagatactg ctgcttgtca
    10441 accacaccga ctaccgctgg tgggcagaag tgcagcagac cccgaaaaga cacagtctgt
    10501 ccagcacaaa gttacttagt ccccagatgt ctggagaaga ggaggattct gacttggcag
    10561 ccaaacttgg aatgtgcaat agagaaatag tacgaagagg ggctctcatt ctcttctgtg
    10621 attatgtctg tcagaacctc catgactccg agcacttaac gtggctcatt gtaaatcaca
    10681 ttcaagatct gatcagcctt tcccacgagc ctccagtaca ggacttcatc agtgccgttc
    10741 atcggaactc tgctgccagc ggcctgttca tccaggcaat tcagtctcgt tgtgaaaacc
    10801 tttcaactcc aaccatgctg aagaaaactc ttcagtgctt ggaggggatc catctcagcc
    10861 agtcgggagc tgtgctcacg ctgtatgtgg acaggcttct gtgcacccct ttccgtgtgc
    10921 tggctcgcat ggtcgacatc cttgcttgtc gccgggtaga aatgcttctg gctgcaaatt
    10981 tacagagcag catggcccag ttgccaatgg aagaactcaa cagaatccag gaataccttc
    11041 agagcagcgg gctcgctcag agacaccaaa ggctctattc cctgctggac aggtttcgtc
    11101 tctccaccat gcaagactca cttagtccct ctcctccagt ctcttcccac ccgctggacg
    11161 gggatgggca cgtgtcactg gaaacagtga gtccggacaa agactggtac gttcatcttg
    11221 tcaaatccca gtgttggacc aggtcagatt ctgcactgct ggaaggtgca gagctggtga
    11281 atcggattcc tgctgaagat atgaatgcct tcatgatgaa ctcggagttc aacctaagcc
    11341 tgctagctcc atgcttaagc ctagggatga gtgaaatttc tggtggccag aagagtgccc
    11401 tttttgaagc agcccgtgag gtgactctgg cccgtgtgag cggcaccgtg cagcagctcc
    11461 ctgctgtcca tcatgtcttc cagcccgagc tgcctgcaga accggcggcc tactggagca
    11521 agttgaatga tctgtttggg gatgctgcac tgtatcagtc cctgcccact ctggcccggg
    11581 ccctggcaca gtacctggtg gtggtctcca aactgcccag tcatttgcac cttcctcctg
    11641 agaaagagaa ggacattgtg aaattcgtgg tggcaaccct tgaggccctg tcctggcatt
    11701 tgatccatga gcagatcccg ctgagtctgg atctccaggc agggctggac tgctgctgcc
    11761 tggccctgca gctgcctggc ctctggagcg tggtctcctc cacagagttt gtgacccacg
    11821 cctgctccct catccactgt gtgcacttca tcctggaggc cgttgcagtg cagcctggag
    11881 agcagcttct tagtccagaa agaaggacaa ataccccaaa agccatcagc gaggaggagg
    11941 aggaagtaga tccaaacaca cagaatccta agtatatcac tgcagcctgt gagatggtgg
    12001 cagaaatggt ggagtctctg cagtcggtgt tggccttggg tcataaaagg aatagcggcg
    12061 tgccggcgtt tctcacgcca ttgctcagga acatcatcat cagcctggcc cgcctgcccc
    12121 ttgtcaacag ctacacacgt gtgcccccac tggtgtggaa gcttggatgg tcacccaaac
    12181 cgggagggga ttttggcaca gcattccctg agatccccgt ggagttcctc caggaaaagg
    12241 aagtctttaa ggagttcatc taccgcatca acacactagg ctggaccagt cgtactcagt
    12301 ttgaagaaac ttgggccacc ctccttggtg tcctggtgac gcagcccctc gtgatggagc
    12361 aggaggagag cccaccagaa gaagacacag agaggaccca gatcaacgtc ctggccgtgc
    12421 aggccatcac ctcactggtg ctcagtgcaa tgactgtgcc tgtggccggc aacccagctg
    12481 taagctgctt ggagcagcag ccccggaaca agcctctgaa agctctcgac accaggtttg
    12541 ggaggaagct gagcattatc agagggattg tggagcaaga gattcaagca atggtttcaa
    12601 agagagagaa tattgccacc catcatttat atcaggcatg ggatcctgtc ccttctctgt
    12661 ctccggctac tacaggtgcc ctcatcagcc acgagaagct gctgctgcag atcaaccccg
    12721 agcgggagct ggggagcatg agctacaaac tcggccaggt gtccatacac tccgtgtggc
    12781 tagggaacag catcacaccc ctgagggagg aggaatggga cgaggaagag gaggaggagg
    12841 ccgacgcccc tgcaccttcg tcaccaccca cgtctccagt caactccagg aaacaccggg
    12901 ctggagttga catccactcc tgttcgcagt ttttgcttga gttgtacagc cgctggatcc
    12961 tgccgtccag ctcagccagg aggaccccgg ccatcctgat cagtgaggtg gtcagatccc
    13021 ttctagtggt ctcagacttg ttcaccgagc gcaaccagtt tgagctgatg tatgtgacgc
    13081 tgacagaact gcgaagggtg cacccttcag aagacgagat cctcgctcag tacctggtgc
    13141 ctgccacctg caaggcagct gccgtccttg ggatggacaa ggccgtggcg gagcctgtca
    13201 gccgcctgct ggagagcacg ctcaggagca gccacctgcc cagcagggtt ggagccctgc
    13261 acggcatcct ctatgtgctg gagtgcgacc tgctggacga cactgccaag cagctcatcc
    13321 cggtcatcag cgactatctc ctctccaacc tgaaagggat cgcccactgc gtgaacattc
    13381 acagccagca gcacgtactg gtcatgtgtg ccactgcgtt ttacctcatt gagaactatc
    13441 ctctggacgt agggccggaa ttttcagcat caataataca gatgtgtggg gtgatgctgt
    13501 ctggaagtga ggagtccacc ccctccatca tttaccactg tgccctcaga ggcctggagc
    13561 gcctcctgct ctctgagcag ctctcccgcc tggatgcaga atcgctggtc aagctgagtg
    13621 tggacagagt gaacgtgcac agcccgcacc gggccatggc ggctctgggc ctgatgctca
    13681 cctgcatgta cacaggaaag gagaaagtca gtccgggtag aacttcagac cctaatcctg
    13741 cagcccccga cagcgagtca gtgattgttg ctatggagcg ggtatctgtt ctttttgata
    13801 ggatcaggaa aggctttcct tgtgaagcca gagtggtggc caggatcctg ccccagtttc
    13861 tagacgactt cttcccaccc caggacatca tgaacaaagt catcggagag tttctgtcca
    13921 accagcagcc atacccccag ttcatggcca ccgtggtgta taaggtgttt cagactctgc
    13981 acagcaccgg gcagtcgtcc atggtccggg actgggtcat gctgtccctc tccaacttca
    14041 cgcagagggc cccggtcgcc atggccacgt ggagcctctc ctgcttcttt gtcagcgcgt
    14101 ccaccagccc gtgggtcgcg gcgatcctcc cacatgtcat cagcaggatg ggcaagctgg
    14161 agcaggtgga cgtgaacctt ttctgcctgg tcgccacaga cttctacaga caccagatag
    14221 aggaggagct cgaccgcagg gccttccagt ctgtgcttga ggtggttgca gccccaggaa
    14281 gcccatatca ccggctgctg acttgtttac gaaatgtcca caaggtcacc acctgctaaa
    14341 acctctactt ccaatcgcac catcatcacc atcaccatca ccaccatgat tacaaggatg
    14401 acgacgataa gtgaggcggc cgccaagctt gatatcaagc ttatcgataa tcaacctctg
    14461 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    14521 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    14581 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    14641 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    14701 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    14761 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    14821 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    14881 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    14941 cttccttccc gcgggcgccc ccctcctcac ggcgagcgaa gcttgtcgag aagtactaga
    15001 ggatctctag agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atcataatca
    15061 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    15121 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    15181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    15241 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcatcac
    15301 tttccaagtc ggttcatctc tatgtctgta taaatctgtc ttttcttggt gtgctttaat
    15361 ttaatgcaaa gatggatacc aactcggaga accaagaata gtccaatgat taaccctatg
    15421 ataaagaaaa aagaggcaat agagcttttc caactactga accaaccttc tacaagctcg
    15481 attggatttt tggatagccc agtatcaccc atcgcgcccg atggtgggac ggtatgaata
    15541 atccggaata tttataggtt tttttattac aaaactgtta cgaaaacagt aaaatactta
    15601 tttatttgcg agatggttat cattttaatt atctccatga tcctaggaga tccgaaccag
    15661 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
    15721 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
    15781 ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg
    15841 ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact
    15901 tt
//
