LOCUS       sequence-209903-       15911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intermediate vector containing HTT gene, except for polyQ region. 
            Used to clone various polyQ lengths into HTT sequence with C-term 
            FLAG tag. Baculovirus transfer vector for insect and mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15911)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C entry vector
FEATURES             Location/Qualifiers
     source          1..15911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     399..420
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        584..688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(689..757,758..1549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(907..926)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1720..2308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2209..2228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2462..2479
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4161..4209
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4234..4253
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4315..4694
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4695..4898
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4849..4869
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4945..5054
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      13627..13636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14322..14345
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14367..14396
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14397..14420
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14511..14531)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14929..14940)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15165..15299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15202..15221)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15256..15275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(15309..15341)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(15559..15650)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(15617..15634)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     mobile_element  15664..15829
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagca gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtaccg catgctggat ctgtacgggc cagatatacg cgttgacatt
     4321 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4381 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     4441 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     4501 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
     4561 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     4621 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     4681 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     4741 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     4801 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4861 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
     4921 ctgcttactg gcttatcgaa tacggacctt taattcaacc caacacaata tattatagtt
     4981 aaataagaat tattatcaaa tcatttgtat attaattaaa atactatact gtaaattaca
     5041 ttttatttac aatcactcga cggatcctta agaaggagat atactatggc gaccctggaa
     5101 aagcgctgac cttagtcgct aacctgcagg agccgctgca ccgaccaaag aaagaacttt
     5161 cagctaccaa gaaagaccgt gtgaatcatt gtctgacaat atgtgaaaac atagtggcac
     5221 agtctgtcag aaattctcca gaatttcaga aacttctggg catcgctatg gaactttttc
     5281 tgctgtgcag tgatgacgca gagtcagatg tcaggatggt ggctgacgaa tgcctcaaca
     5341 aagttatcaa agctttgatg gattctaatc ttccaaggtt acagctcgag ctctataagg
     5401 aaattaaaaa gaatggtgcc cctcggagtt tgcgtgctgc cctgtggagg tttgctgagc
     5461 tggctcacct ggttcggcct cagaaatgca ggccttacct ggtgaacctt ctgccgtgcc
     5521 tgactcgaac aagcaagaga cccgaagaat cagtccagga gaccttggct gcagctgttc
     5581 ccaaaattat ggcttctttt ggcaattttg caaatgacaa tgaaattaag gttttgttaa
     5641 aggccttcat agcgaacctg aagtcaagct cccccaccat tcggcggaca gcggctggat
     5701 cagcagtgag catctgccag cactcaagaa ggacacaata tttctatagt tggctactaa
     5761 atgtgctctt aggcttactc gttcctgtcg aggatgaaca ctccactctg ctgattcttg
     5821 gcgtgctgct caccctgagg tatttggtgc ccttgctgca gcagcaggtc aaggacacaa
     5881 gcctgaaagg cagcttcgga gtgacaagga aagaaatgga agtctctcct tctgcagagc
     5941 agcttgtcca ggtttatgaa ctgacgttac atcatacaca gcaccaagac cacaatgttg
     6001 tgaccggagc cctggagctg ttgcagcagc tcttcagaac gcctccaccc gagcttctgc
     6061 aaaccctgac cgcagtcggg ggcattgggc agctcaccgc tgctaaggag gagtctggtg
     6121 gccgaagccg tagtgggagt attgtggaac ttatagctgg agggggttcc tcatgcagcc
     6181 ctgtcctttc aagaaaacaa aaaggcaaag tgctcttagg agaagaagaa gccttggagg
     6241 atgactctga atcgagatcg gatgtcagca gctctgcctt aacagcctca gtgaaggatg
     6301 agatcagtgg agagctggct gcttcttcag gggtttccac tccagggtca gcaggtcatg
     6361 acatcatcac agaacagcca cggtcacagc acacactgca ggcggactca gtggatctgg
     6421 ccagctgtga cttgacaagc tctgccactg atggggatga ggaggatatc ttgagccaca
     6481 gctccagcca ggtcagcgcc gtcccatctg accctgccat ggacctgaat gatgggaccc
     6541 aggcctcgtc gcccatcagc gacagctccc agaccaccac cgaagggcct gattcagctg
     6601 ttaccccttc agacagttct gaaattgtgt tagacggtac cgacaaccag tatttgggcc
     6661 tgcagattgg acagccccag gatgaagatg aggaagccac aggtattctt cctgatgaag
     6721 cctcggaggc cttcaggaac tcttccatgg cccttcaaca ggcacattta ttgaaaaaca
     6781 tgagtcactg caggcagcct tctgacagca gtgttgataa atttgtgttg agagatgaag
     6841 ctactgaacc gggtgatcaa gaaaacaagc cttgccgcat caaaggtgac attggacagt
     6901 ccactgatga tgactctgca cctcttgtcc attgtgtccg ccttttatct gcttcgtttt
     6961 tgctaacagg gggaaaaaat gtgctggttc cggacaggga tgtgagggtc agcgtgaagg
     7021 ccctggccct cagctgtgtg ggagcagctg tggccctcca cccggaatct ttcttcagca
     7081 aactctataa agttcctctt gacaccacgg aataccctga ggaacagtat gtctcagaca
     7141 tcttgaacta catcgatcat ggagacccac aggttcgagg agccactgcc attctctgtg
     7201 ggaccctcat ctgctccatc ctcagcaggt cccgcttcca cgtgggagat tggatgggca
     7261 ccattagaac cctcacagga aatacatttt ctttggcgga ttgcattcct ttgctgcgga
     7321 aaacactgaa ggatgagtct tctgttactt gcaagttagc ttgtacagct gtgaggaact
     7381 gtgtcatgag tctctgcagc agcagctaca gtgagttagg actgcagctg atcatcgatg
     7441 tgctgactct gaggaacagt tcctattggc tggtgaggac agagcttctg gaaacccttg
     7501 cagagattga cttcaggctg gtgagctttt tggaggcaaa agcagaaaac ttacacagag
     7561 gggctcatca ttatacaggg cttttaaaac tgcaagaacg agtgctcaat aatgttgtca
     7621 tccatttgct tggagatgaa gaccccaggg tgcgacatgt tgccgcagca tcactaatta
     7681 ggcttgtccc aaagctgttt tataaatgtg accaaggaca agctgatcca gtagtggccg
     7741 tggcaagaga tcaaagcagt gtttacctga aacttctcat gcatgagacg cagcctccat
     7801 ctcatttctc cgtcagcaca ataaccagaa tatatagagg ctataaccta ctaccaagca
     7861 taacagacgt cactatggaa aataaccttt caagagttat tgcagcagtt tctcatgaac
     7921 taatcacatc aaccaccaga gcactcacat ttggatgctg tgaagctttg tgtcttcttt
     7981 ccactgcctt cccagtttgc atttggagtt taggttggca ctgtggagtg cctccactga
     8041 gtgcctcaga tgagtctagg aagagctgta ccgttgggat ggccacaatg attctgaccc
     8101 tgctctcgtc agcttggttc ccattggatc tctcagccca tcaagatgct ttgattttgg
     8161 ccggaaactt gcttgcagcc agtgctccca aatctctgag aagttcatgg gcctctgaag
     8221 aagaagccaa cccagcagcc accaagcaag aggaggtctg gccagccctg ggggaccggg
     8281 ccctggtgcc catggtggag cagctcttct ctcacctgct gaaggtgatt aacatttgtg
     8341 cccacgtcct ggatgacgtg gctcctggac ccgcaataaa ggcagccttg ccttctctaa
     8401 caaacccccc ttctctaagt cccatccgac gaaaggggaa ggagaaagaa ccaggagaac
     8461 aagcatctgt accgttgagt cccaagaaag gcagtgaggc cagtgcagct tctagacaat
     8521 ctgatacctc aggtcctgtt acaacaagta aatcctcatc actggggagt ttctatcatc
     8581 ttccttcata cctcaaactg catgatgtcc tgaaagctac acacgctaac tacaaggtca
     8641 cgctggatct tcagaacagc acggaaaagt ttggagggtt tcttcgctca gccttggatg
     8701 ttctttctca gatactagag ctggccacac tgcaggacat tgggaagtgt gttgaagaga
     8761 tcctaggata cctgaaatcc tgctttagtc gagaaccaat gatggcaact gtttgtgttc
     8821 aacaattgtt gaagactctc tttggcacaa acttggcctc ccagtttgat ggcttatctt
     8881 ccaaccccag caagtcacaa ggccgagcac agcgccttgg ctcctccagt gtgaggccag
     8941 gcttgtacca ctactgcttc atggccccgt acacccactt cacccaggcc ctcgctgacg
     9001 ccagcctgag gaacatggtg caggcggagc aggagaacga cacctcggga tggtttgatg
     9061 tcctccagaa agtgtctacc cagttgaaga caaacctcac gagtgtcaca aagaaccgtg
     9121 cagataagaa tgctattcat aatcacattc gtttgtttga acctcttgtt ataaaagctt
     9181 taaaacagta cacgactaca acatgtgtgc agttacagaa gcaggtttta gatttgctgg
     9241 cgcagctggt tcagttacgg gttaattact gtcttctgga ttcagatcag gtgtttattg
     9301 gctttgtatt gaaacagttt gaatacattg aagtgggcca gttcagggaa tcagaggcaa
     9361 tcattccaaa catctttttc ttcttggtat tactatctta tgaacgctat cattcaaaac
     9421 agatcattgg aattcctaaa atcattcagc tctgtgatgg catcatggcc agtggaagga
     9481 aggctgtgac acatgccata ccggctctgc agcccatagt ccacgacctc tttgtattaa
     9541 gaggaacaaa taaagctgat gcaggaaaag agcttgaaac ccaaaaagag gtggtggtgt
     9601 caatgttact gagactcatc cagtaccatc aggtgttgga gatgttcatt cttgtcctgc
     9661 agcagtgcca caaggagaat gaagacaagt ggaagcgact gtctcgacag atagctgaca
     9721 tcatcctccc aatgttagcc aaacagcaga tgcacattga ctctcatgaa gcccttggag
     9781 tgttaaatac attatttgag attttggccc cttcctccct ccgtccggta gacatgcttt
     9841 tacggagtat gttcgtcact ccaaacacaa tggcgtccgt gagcactgtt caactgtgga
     9901 tatcgggaat tctggccatt ttgagggttc tgatttccca gtcaactgaa gatattgttc
     9961 tttctcgtat tcaggagctc tccttctctc cgtatttaat ctcctgtaca gtaattaata
    10021 ggttaagaga tggggacagt acttcaacgc tagaagaaca cagtgaaggg aaacaaataa
    10081 agaatttgcc agaagaaaca ttttcaaggt ttctattaca actggttggt attcttttag
    10141 aagacattgt tacaaaacag ctgaaggtgg aaatgagtga gcagcaacat actttctatt
    10201 gccaggaact aggcacactg ctaatgtgtc tgatccacat cttcaagtct ggaatgttcc
    10261 ggagaatcac agcagctgcc actaggctgt tccgcagtga tggctgtggc ggcagtttct
    10321 acaccctgga cagcttgaac ttgcgggctc gttccatgat caccacccac ccggccctgg
    10381 tgctgctctg gtgtcagata ctgctgcttg tcaaccacac cgactaccgc tggtgggcag
    10441 aagtgcagca gaccccgaaa agacacagtc tgtccagcac aaagttactt agtccccaga
    10501 tgtctggaga agaggaggat tctgacttgg cagccaaact tggaatgtgc aatagagaaa
    10561 tagtacgaag aggggctctc attctcttct gtgattatgt ctgtcagaac ctccatgact
    10621 ccgagcactt aacgtggctc attgtaaatc acattcaaga tctgatcagc ctttcccacg
    10681 agcctccagt acaggacttc atcagtgccg ttcatcggaa ctctgctgcc agcggcctgt
    10741 tcatccaggc aattcagtct cgttgtgaaa acctttcaac tccaaccatg ctgaagaaaa
    10801 ctcttcagtg cttggagggg atccatctca gccagtcggg agctgtgctc acgctgtatg
    10861 tggacaggct tctgtgcacc cctttccgtg tgctggctcg catggtcgac atccttgctt
    10921 gtcgccgggt agaaatgctt ctggctgcaa atttacagag cagcatggcc cagttgccaa
    10981 tggaagaact caacagaatc caggaatacc ttcagagcag cgggctcgct cagagacacc
    11041 aaaggctcta ttccctgctg gacaggtttc gtctctccac catgcaagac tcacttagtc
    11101 cctctcctcc agtctcttcc cacccgctgg acggggatgg gcacgtgtca ctggaaacag
    11161 tgagtccgga caaagactgg tacgttcatc ttgtcaaatc ccagtgttgg accaggtcag
    11221 attctgcact gctggaaggt gcagagctgg tgaatcggat tcctgctgaa gatatgaatg
    11281 ccttcatgat gaactcggag ttcaacctaa gcctgctagc tccatgctta agcctaggga
    11341 tgagtgaaat ttctggtggc cagaagagtg ccctttttga agcagcccgt gaggtgactc
    11401 tggcccgtgt gagcggcacc gtgcagcagc tccctgctgt ccatcatgtc ttccagcccg
    11461 agctgcctgc agaaccggcg gcctactgga gcaagttgaa tgatctgttt ggggatgctg
    11521 cactgtatca gtccctgccc actctggccc gggccctggc acagtacctg gtggtggtct
    11581 ccaaactgcc cagtcatttg caccttcctc ctgagaaaga gaaggacatt gtgaaattcg
    11641 tggtggcaac ccttgaggcc ctgtcctggc atttgatcca tgagcagatc ccgctgagtc
    11701 tggatctcca ggcagggctg gactgctgct gcctggccct gcagctgcct ggcctctgga
    11761 gcgtggtctc ctccacagag tttgtgaccc acgcctgctc cctcatccac tgtgtgcact
    11821 tcatcctgga ggccgttgca gtgcagcctg gagagcagct tcttagtcca gaaagaagga
    11881 caaatacccc aaaagccatc agcgaggagg aggaggaagt agatccaaac acacagaatc
    11941 ctaagtatat cactgcagcc tgtgagatgg tggcagaaat ggtggagtct ctgcagtcgg
    12001 tgttggcctt gggtcataaa aggaatagcg gcgtgccggc gtttctcacg ccattgctca
    12061 ggaacatcat catcagcctg gcccgcctgc cccttgtcaa cagctacaca cgtgtgcccc
    12121 cactggtgtg gaagcttgga tggtcaccca aaccgggagg ggattttggc acagcattcc
    12181 ctgagatccc cgtggagttc ctccaggaaa aggaagtctt taaggagttc atctaccgca
    12241 tcaacacact aggctggacc agtcgtactc agtttgaaga aacttgggcc accctccttg
    12301 gtgtcctggt gacgcagccc ctcgtgatgg agcaggagga gagcccacca gaagaagaca
    12361 cagagaggac ccagatcaac gtcctggccg tgcaggccat cacctcactg gtgctcagtg
    12421 caatgactgt gcctgtggcc ggcaacccag ctgtaagctg cttggagcag cagccccgga
    12481 acaagcctct gaaagctctc gacaccaggt ttgggaggaa gctgagcatt atcagaggga
    12541 ttgtggagca agagattcaa gcaatggttt caaagagaga gaatattgcc acccatcatt
    12601 tatatcaggc atgggatcct gtcccttctc tgtctccggc tactacaggt gccctcatca
    12661 gccacgagaa gctgctgctg cagatcaacc ccgagcggga gctggggagc atgagctaca
    12721 aactcggcca ggtgtccata cactccgtgt ggctagggaa cagcatcaca cccctgaggg
    12781 aggaggaatg ggacgaggaa gaggaggagg aggccgacgc ccctgcacct tcgtcaccac
    12841 ccacgtctcc agtcaactcc aggaaacacc gggctggagt tgacatccac tcctgttcgc
    12901 agtttttgct tgagttgtac agccgctgga tcctgccgtc cagctcagcc aggaggaccc
    12961 cggccatcct gatcagtgag gtggtcagat cccttctagt ggtctcagac ttgttcaccg
    13021 agcgcaacca gtttgagctg atgtatgtga cgctgacaga actgcgaagg gtgcaccctt
    13081 cagaagacga gatcctcgct cagtacctgg tgcctgccac ctgcaaggca gctgccgtcc
    13141 ttgggatgga caaggccgtg gcggagcctg tcagccgcct gctggagagc acgctcagga
    13201 gcagccacct gcccagcagg gttggagccc tgcacggcat cctctatgtg ctggagtgcg
    13261 acctgctgga cgacactgcc aagcagctca tcccggtcat cagcgactat ctcctctcca
    13321 acctgaaagg gatcgcccac tgcgtgaaca ttcacagcca gcagcacgta ctggtcatgt
    13381 gtgccactgc gttttacctc attgagaact atcctctgga cgtagggccg gaattttcag
    13441 catcaataat acagatgtgt ggggtgatgc tgtctggaag tgaggagtcc accccctcca
    13501 tcatttacca ctgtgccctc agaggcctgg agcgcctcct gctctctgag cagctctccc
    13561 gcctggatgc agaatcgctg gtcaagctga gtgtggacag agtgaacgtg cacagcccgc
    13621 accgggccat ggcggctctg ggcctgatgc tcacctgcat gtacacagga aaggagaaag
    13681 tcagtccggg tagaacttca gaccctaatc ctgcagcccc cgacagcgag tcagtgattg
    13741 ttgctatgga gcgggtatct gttctttttg ataggatcag gaaaggcttt ccttgtgaag
    13801 ccagagtggt ggccaggatc ctgccccagt ttctagacga cttcttccca ccccaggaca
    13861 tcatgaacaa agtcatcgga gagtttctgt ccaaccagca gccatacccc cagttcatgg
    13921 ccaccgtggt gtataaggtg tttcagactc tgcacagcac cgggcagtcg tccatggtcc
    13981 gggactgggt catgctgtcc ctctccaact tcacgcagag ggccccggtc gccatggcca
    14041 cgtggagcct ctcctgcttc tttgtcagcg cgtccaccag cccgtgggtc gcggcgatcc
    14101 tcccacatgt catcagcagg atgggcaagc tggagcaggt ggacgtgaac cttttctgcc
    14161 tggtcgccac agacttctac agacaccaga tagaggagga gctcgaccgc agggccttcc
    14221 agtctgtgct tgaggtggtt gcagccccag gaagcccata tcaccggctg ctgacttgtt
    14281 tacgaaatgt ccacaaggtc accacctgcg gtggaagcgg tgactacaaa gacgatgacg
    14341 acaagtaaaa cctctacttc caatcgcacc atcatcacca tcaccatcac caccatgatt
    14401 acaaggatga cgacgataag tgaggcggcc gccaagcttg atatcaagct tatcgataat
    14461 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
    14521 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
    14581 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
    14641 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
    14701 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
    14761 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
    14821 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
    14881 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
    14941 ccagcggacc ttccttcccg cgggcgcccc cctcctcacg gcgagcgaag cttgtcgaga
    15001 agtactagag gatctctaga gcctgcagtc tcgacaagct tgtcgagaag tactagagga
    15061 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    15121 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    15181 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    15241 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
    15301 gatcatcact ttccaagtcg gttcatctct atgtctgtat aaatctgtct tttcttggtg
    15361 tgctttaatt taatgcaaag atggatacca actcggagaa ccaagaatag tccaatgatt
    15421 aaccctatga taaagaaaaa agaggcaata gagcttttcc aactactgaa ccaaccttct
    15481 acaagctcga ttggattttt ggatagccca gtatcaccca tcgcgcccga tggtgggacg
    15541 gtatgaataa tccggaatat ttataggttt ttttattaca aaactgttac gaaaacagta
    15601 aaatacttat ttatttgcga gatggttatc attttaatta tctccatgat cctaggagat
    15661 ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta
    15721 gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa
    15781 aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt
    15841 ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct
    15901 tcggctactt t
//
