LOCUS       sequence-209910-       12748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinase-mediated cassette exchange in ES cells to insert a Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 12748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Ai62(TITL-tdT) Flp-in replacement vector
FEATURES             Location/Qualifiers
     source          1..12748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..50)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     150..169
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     363..385
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     377..394
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     378..394
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    413..446
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        3008..3322
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3016..3034
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3052..3070
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3087..3105
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3123..3141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3159..3177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3194..3212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3230..3248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3295..3319
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3332..3365)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3442..3490
                     /note="synthetic polyadenylation signal"
     polyA_signal    3499..3975
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3698..3717)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     polyA_signal    3987..4448
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(4475..4508)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4535..4544
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4674..4692)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4942..4961
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5136..5159
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5996..6584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6049..6069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6467..6478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6609..6626)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6615..6822
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9320..9353
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9380..9879
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9415..9438)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9812..9831
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(10527..10543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10527..10543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10540..10562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10551..10567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10575..10581,10582..10599,10600..10605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10620..10641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10758..10775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10929..11517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11009..11028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11688..12479,12480..12548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12311..12330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12549..12653)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12721..12739
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
       61 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
      121 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
      181 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
      241 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      301 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      361 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta ccgaagttcc
      421 tattcttcaa atagtatagg aacttctttc ggccggccaa tcgattggat ccgttttatg
      481 ccgcggttac cgtgaagata tcttaattaa gaattcgagc tcgctagagc cccatcctca
      541 ctgactccgt cctggagttg gatgagagat aatggcctta cgttgtgcca ggggagggtc
      601 gggctggatt tagcaagatt taccttctcc aaagagcggt gctgcagtgg cacagctgcc
      661 cacggaggtg ggggggtcac cgtccctgga ggtgatgaag aactgtgggg atgtggcact
      721 gagggacatg gccagtgggc acggtgggtg ggttggggtt ggtcttgggg atcttggagg
      781 gcttttccag ccttcatgat ttgacgattg tatgaacatc tacatggcaa ttctccagct
      841 gcctgtccca gtcctactga cccagctgta tctctccagg caagctcttc caccccttct
      901 gcttgcatcc agacaccatc aaacatgcag gctcagacac agggaccagc agtgtctgtg
      961 gcctttttgt gctcctctcc atgctgggtt ttaacttgct ctttgtcctt ctatcctatc
     1021 ttcttatcct taaggctgtt ctgaacgctg tgacttggag agtgtcccag agccctcaac
     1081 acctgcatgt cccacgtcca tgctgtcctg cacttcctta tccccaagat ctgcctctcc
     1141 gtgatgcact gaattggcaa acatgtgtca ccccagacca acaatgtcac agcaaactcc
     1201 cccttgatag gacaaggggg aatggcttta cactgagaca ggggaggttt gggttggata
     1261 tgaggaggca gtttttcccc cagagggtgg tgacgcactg aacaggttgc ccaaggaggc
     1321 tgtggatgcc ccatccctgc aggcattcaa ggccaggctg gatgtggctc tgggcagcct
     1381 gggctgctgg ttgatgaccc tgcacatagc agggggttgg atctggatga gcactgtgct
     1441 cctttgcaac ccaggccgtt ctatgattct gtcattctaa atctctcttt cagcctaaag
     1501 ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga
     1561 ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg
     1621 gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta
     1681 gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc cctctagcga
     1741 gctcggtacc cagagcccca tcctcactga ctccgtcctg gagttggatg agagataatg
     1801 gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc ttctccaaag
     1861 agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc cctggaggtg
     1921 atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg tgggtgggtt
     1981 ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga cgattgtatg
     2041 aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca gctgtatctc
     2101 tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac atgcaggctc
     2161 agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc tgggttttaa
     2221 cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga acgctgtgac
     2281 ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct gtcctgcact
     2341 tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat gtgtcacccc
     2401 agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg gctttacact
     2461 gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga gggtggtgac
     2521 gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc attcaaggcc
     2581 aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca catagcaggg
     2641 ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat gattctgtca
     2701 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     2761 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     2821 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     2881 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     2941 ttgggggggg gctgtccctc taggggtacc cggggatcca ccggtatcga ttacctatca
     3001 caaattcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     3061 gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     3121 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     3181 tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
     3241 gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg
     3301 tttagtgaac cgtcagatcg cctatttaaa tataacttcg tatagcatac attatacgaa
     3361 gttattcgcg atgaataaat gaaagcttgc agatcccctc agcaccatgg ctagcggcag
     3421 cctcggagtt tgaatagata gaataaaata tctttatttt cattccatct gtgtgttggt
     3481 tttttgtgtg agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     3541 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     3601 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     3661 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     3721 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     3781 cctcaggctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     3841 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     3901 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     3961 cccttccctg tccttctgcc tcagcgctgt aagtctgcag aaattgatga tctattaaac
     4021 aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga
     4081 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga
     4141 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca
     4201 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca
     4261 ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac
     4321 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
     4381 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
     4441 catcagaaag cttttaatta actgaatagc taacataact tcgtatagca tacattatac
     4501 gaagttatca cgcgtgtttg ggacgcgtgc tagcgccacc atggtgagca agggcgagga
     4561 ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
     4621 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     4681 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
     4741 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     4801 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     4861 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     4921 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     4981 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     5041 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     5101 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     5161 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg
     5221 gcatggtacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa
     5281 catggccgtg attaaggagt ttatgaggtt taaagtcagg atggagggca gcatgaacgg
     5341 gcacgagttt gagattgaag gagaggggga agggaggccc tatgagggca cccagacagc
     5401 aaagctgaaa gtgacaaagg gcggccctct gccattcgca tgggacatcc tgtctcctca
     5461 gtttatgtat ggaagtaaag cctacgtgaa acaccctgct gacattccag attacaagaa
     5521 actgagcttc ccagaggggt ttaagtggga aagagtgatg aacttcgaag acgggggcct
     5581 ggtgactgtc acccaggaca gctctctgca ggacggcact ctgatctata aggtgaaaat
     5641 gcgcggcacc aattttcccc ctgatggccc agtcatgcag aagaagacaa tgggctggga
     5701 ggcttcaaca gaaaggctgt accccagaga cggcgtgctg aagggagaga tccaccaggc
     5761 actgaagctg aaagatggag ggcattatct ggtcgaattc aaaaccatct acatggccaa
     5821 gaaacccgtg cagctgcctg gctactatta cgtcgacaca aagctggata ttactagcca
     5881 taacgaagac tacaccattg tggagcagta tgagcggagc gaggggaggc accatctgtt
     5941 tctgtacggc atggacgagc tgtacaagta aacgcgttac gtaaagcttg tcgataatca
     6001 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6061 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6121 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6181 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6241 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6301 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6361 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta
     6421 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6481 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6541 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgac accgagcgct
     6601 gctcgaggcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     6661 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     6721 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     6781 caagggggag gattgggaag acaatagcag gcatgctggg gacacgtggg atcctctaga
     6841 gccccatcct cactgactcc gtcctggagt tggatgagag ataatggcct tacgttgtgc
     6901 caggggaggg tcgggctgga tttagcaaga tttaccttct ccaaagagcg gtgctgcagt
     6961 ggcacagctg cccacggagg tgggggggtc accgtccctg gaggtgatga agaactgtgg
     7021 ggatgtggca ctgagggaca tggccagtgg gcacggtggg tgggttgggg ttggtcttgg
     7081 ggatcttgga gggcttttcc agccttcatg atttgacgat tgtatgaaca tctacatggc
     7141 aattctccag ctgcctgtcc cagtcctact gacccagctg tatctctcca ggcaagctct
     7201 tccacccctt ctgcttgcat ccagacacca tcaaacatgc aggctcagac acagggacca
     7261 gcagtgtctg tggccttttt gtgctcctct ccatgctggg ttttaacttg ctctttgtcc
     7321 ttctatccta tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc
     7381 agagccctca acacctgcat gtcccacgtc catgctgtcc tgcacttcct tatccccaag
     7441 atctgcctct ccgtgatgca ctgaattggc aaacatgtgt caccccagac caacaatgtc
     7501 acagcaaact cccccttgat aggacaaggg ggaatggctt tacactgaga caggggaggt
     7561 ttgggttgga tatgaggagg cagtttttcc cccagagggt ggtgacgcac tgaacaggtt
     7621 gcccaaggag gctgtggatg ccccatccct gcaggcattc aaggccaggc tggatgtggc
     7681 tctgggcagc ctgggctgct ggttgatgac cctgcacata gcagggggtt ggatctggat
     7741 gagcactgtg ctcctttgca acccaggccg ttctatgatt ctgtcattct aaatctctct
     7801 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     7861 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     7921 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     7981 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     8041 tccctctaga gccccatcct cactgactcc gtcctggagt tggatgagag ataatggcct
     8101 tacgttgtgc caggggaggg tcgggctgga tttagcaaga tttaccttct ccaaagagcg
     8161 gtgctgcagt ggcacagctg cccacggagg tgggggggtc accgtccctg gaggtgatga
     8221 agaactgtgg ggatgtggca ctgagggaca tggccagtgg gcacggtggg tgggttgggg
     8281 ttggtcttgg ggatcttgga gggcttttcc agccttcatg atttgacgat tgtatgaaca
     8341 tctacatggc aattctccag ctgcctgtcc cagtcctact gacccagctg tatctctcca
     8401 ggcaagctct tccacccctt ctgcttgcat ccagacacca tcaaacatgc aggctcagac
     8461 acagggacca gcagtgtctg tggccttttt gtgctcctct ccatgctggg ttttaacttg
     8521 ctctttgtcc ttctatccta tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg
     8581 agagtgtccc agagccctca acacctgcat gtcccacgtc catgctgtcc tgcacttcct
     8641 tatccccaag atctgcctct ccgtgatgca ctgaattggc aaacatgtgt caccccagac
     8701 caacaatgtc acagcaaact cccccttgat aggacaaggg ggaatggctt tacactgaga
     8761 caggggaggt ttgggttgga tatgaggagg cagtttttcc cccagagggt ggtgacgcac
     8821 tgaacaggtt gcccaaggag gctgtggatg ccccatccct gcaggcattc aaggccaggc
     8881 tggatgtggc tctgggcagc ctgggctgct ggttgatgac cctgcacata gcagggggtt
     8941 ggatctggat gagcactgtg ctcctttgca acccaggccg ttctatgatt ctgtcattct
     9001 aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
     9061 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
     9121 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
     9181 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
     9241 gggggggctg tccctctaga gtcgacggcg cgccgtaaaa gcttggcata gatcttattg
     9301 cagaagccat atgttttagg tgccagggcg tgcccttggg ctccccgggc gcgtttgggc
     9361 ggccgcatcg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     9421 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     9481 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     9541 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     9601 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     9661 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     9721 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     9781 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     9841 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcaatcg ccgctagccg
     9901 ccaccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     9961 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
    10021 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
    10081 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
    10141 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
    10201 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
    10261 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
    10321 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
    10381 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgt aagtgctagc ccctaggcct
    10441 gaatcgaagt tcctattctt caaaaggtat aggaacttcg agctcgtcga ctgcagaggc
    10501 ctgcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10561 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10621 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    10681 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    10741 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    10801 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    10861 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    10921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    10981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    11161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    11221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11341 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    11401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    11581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    11641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    11701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    11761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    11821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    11881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    11941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    12001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    12061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    12121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    12181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    12241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    12301 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    12361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    12421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    12481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    12661 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    12721 aataggcgta tcacgaggcc ctttcgtc
//
