LOCUS       sequence-209925-        5028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The core promoter region of FTO was inserted into pGL3 basic vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Su R, Dong L, Li C, Nachtergaele S, Wunderlich M, Qing Y, Deng X, 
            Wang Y, Weng X, Hu C, Yu M, Skibbe J, Dai Q, Zou D, Wu T, Yu K, Weng
            H, Huang H, Ferchen K, Qin X, Zhang B, Qi J, Sasaki AT, Plas DR, 
            Bradner JE, Wei M, Marcucci G, Jiang X, Mulloy JC, Jin J, He C, Chen
            J
  TITLE     R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m(6)A/MYC/CEBPA 
            Signaling.
  JOURNAL   Cell. 2018 Jan 11;172(1-2):90-105.e23. doi: 
            10.1016/j.cell.2017.11.031. Epub 2017 Dec 14.
  PUBMED    29249359
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2018-01-11"; volume: "172(1-2):90-105.e23. doi"; pages: " 
            10.1016/j.cell.2017.11.031. Epub 2017 Dec 14"
COMMENT     Sequence Label: pGL3-basic-FTO promoter_WT
FEATURES             Location/Qualifiers
     source          1..5028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      292..301
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             298..1950
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(364..382)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1991..2112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2002..2021)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2056..2075
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2360..2377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2531..3119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2611..2630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3290..4081,4082..4150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3913..3932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4151..4255)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4282..4737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4369..4388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4579..4600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4868..4916
                     /note="synthetic polyadenylation signal"
     misc_feature    4930..5021
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4970..4989
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtacctcca cccaccctca tcctccccca tcctcccggg tactcaccgt gccactggcc
       61 ctgcagctag ctaccgttgc tatagcgccg acagcgtggc gggcggctgg ccgagaggag
      121 cacgggagaa acatggcagg ctcccgtagc ctcctgggaa atgtagttct ccttggactc
      181 tagcctgttt gctcgcgggg tagcggacta cgctcttcca gctgtcggac ctgggaaatt
      241 cctcgagatc tgcgatctaa gtaagcttgg cattccggta ctgttggtaa agccaccatg
      301 gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc
      361 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct
      421 tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt
      481 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc
      541 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca
      601 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg
      661 cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
      721 aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt
      781 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt
      841 gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct
      901 actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat
      961 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt
     1021 ccattccatc acggttttgg aatgtttact acactcggat atttgatatg tggatttcga
     1081 gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag
     1141 attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt
     1201 gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag
     1261 gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg
     1321 ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg
     1381 gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg
     1441 ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat
     1501 gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct acattctgga
     1561 gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg
     1621 attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac
     1681 cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc
     1741 gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac
     1801 gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa
     1861 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
     1921 gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga
     1981 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2041 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2101 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     2161 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg
     2221 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     2281 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     2341 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2401 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2461 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2521 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2581 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2641 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2701 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2761 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2821 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2881 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2941 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3001 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3061 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3121 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3181 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3241 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3301 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3361 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3421 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3481 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3541 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3601 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3661 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3721 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3781 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3841 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3901 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3961 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4021 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4081 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4141 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4201 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4261 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4321 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4381 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4441 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4501 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4561 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4621 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4681 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc
     4741 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     4801 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag
     4861 cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     4921 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
     4981 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata
//
