LOCUS       sequence-209926-        8530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High expression of mCherry-EGFP-PIM. Can be used for inducible 
            aggregate formation upon AP20187 addition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Janssen AFJ, Katrukha EA, van Straaten W, Verlhac P, Reggiori F, 
            Kapitein LC
  TITLE     Probing aggrephagy using chemically-induced protein aggregates.
  JOURNAL   Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4.
  PUBMED    30315152
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-10-12"; volume: "9(1):4245. doi"; pages: " 
            10.1038/s41467-018-06674-4"
COMMENT     Sequence Label: pGW1-mCherry-EGFP-PIM
FEATURES             Location/Qualifiers
     source          1..8530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        620..823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     774..794
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     820..844
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1819..2139
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2146..2466
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVEHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDCAYGATGHPGIIPPHTTLVFDVELL
                     KLE"
     CDS             2482..3186
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2624..2642)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2892..2911
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(3196..3198,3199..3909)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3196..3198,3199..3909)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3238..3259)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3499..3518)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3846..3867
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4600..4920
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4927..5247
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4927..5247
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    5372..5506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5409..5428)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5463..5482
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5530..5546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5530..5546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5543..5565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5554..5570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5578..5584,5585..5602,5603..5608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5623..5644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5761..5778)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5932..6520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6012..6031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6691..7482,7483..7551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7314..7333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7761..7779
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7817..7839)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7939..7958
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8190..8212
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8204..8221
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8205..8221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atcgcagttt cgatataggt gacagacgat atgaggctat atcgccgata gaggcgacat
       61 caagctggca catggccaat gcatatcgat ctatacattg aatcaatatt ggccattagc
      121 catattattc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
      181 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaaca gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      841 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      901 cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca agagtgacgt
      961 aagtaccgcc tatagagtct ataggcccac ccccttggct tcttatgcat gctatactgt
     1021 ttttggcttg gggtctatac acccccgctt cctcatgtta taggtgatgg tatagcttag
     1081 cctataggtg tgggttattg accattattg accactcccc tattggtgac gatactttcc
     1141 attactaatc cataacatgg ctctttgcac aactctcttt attggctata tgccaataca
     1201 ctgtccttca gagactgaca cggactctgt atttttacag gatggggtct catttattat
     1261 ttacaaattc acatatacaa caccaccgtc cccagtgccc gcagttttta ttaaacataa
     1321 cgtgggatct ccagcgaatc tcgggtacgt gttccggaca tggggctctt ctccggtagc
     1381 ggcggagctt ctacatccag ccctgctccc atcctcccac tcatggtcct cggcagctcc
     1441 ttgctcctaa cagtggaggc cagacttagg cacagcacga tgcccaccac caccagtgtg
     1501 cccacaaggc cgtggcggta gggtatgtgt ctgaaaatga gctcggggag cgggcttgca
     1561 ccgctgacgc atttggaaga cttaaggcag cggcagaaga agatgcaggc agctgagttg
     1621 ttgtgttctg ataagagtca gaggtaactc ccgttgcggt gctgttaacg gtggagggca
     1681 gtgtagtctg agcagtactc gttgctgccg cgcgcgccac cagacataat agctgacaga
     1741 ctaacagact gttcctttcc atgggtcttt tctgcagtca ccgtccttga cacgaagctt
     1801 gccaccatgc gctctagagg cgtccaagtc gaaaccatta gtcccggcga tggcagaaca
     1861 tttcctaaaa ggggacaaac atgtgtcgtc cattatacag gcatgttgga ggacggcaaa
     1921 aaggtggaca gtagtagaga tcgcaataaa cctttcaaat tcatgttggg aaaacaagaa
     1981 gtcattaggg gatgggagga gggcgtggct caaatgtccg tcggccaacg cgctaagctc
     2041 accatcagcc ccgactacgc atacggcgct accggacatc ccggaattat tccccctcac
     2101 gctaccttgg tgtttgacgt cgaactgttg aagctcgaga ctagaggagt gcaggtggag
     2161 actatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg cgtggagcac
     2221 tacaccggga tgcttgaaga tggaaagaaa gttgattcct cccgggacag aaacaagccc
     2281 tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag
     2341 atgagtgtgg gtcagagagc caaactgact atatctccag attgtgccta tggtgccact
     2401 gggcacccag gcatcatccc accacatacc actctcgtct tcgatgtgga gcttctaaaa
     2461 ctggaaacta gtgaaccggt agtgagcaag ggcgaggagg ataacatggc catcatcaag
     2521 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     2581 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2641 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     2701 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     2761 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2821 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     2881 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     2941 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     3001 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     3061 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     3121 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     3181 tacaagggcg cgcctgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3241 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3301 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3361 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3421 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3481 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3541 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3601 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3661 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     3721 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     3781 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     3841 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3901 ctgtacaagg ctagccgctc acgcggcgtt caggtggaga ctataagccc gggggatggt
     3961 cgcacttttc ctaaacgagg ccagacgtgc gtagttcact ataccggcat gcttgaagac
     4021 ggcaagaagg tggatagctc tagggaccgg aataagccct tcaagttcat gcttggtaag
     4081 caagaagtga tccggggttg ggaagagggg gttgcccaaa tgtcagtcgg acaacgggcg
     4141 aagttgacta tttctcctga ttacgcttat ggtgcgacgg gacatcctgg catcataccg
     4201 cctcacgcca ctcttgtctt tgatgtggaa cttttgaagc tggaaacgcg aggggttcaa
     4261 gttgagacca tttcaccggg cgatggcaga acattcccga aaaggggaca aacgtgtgta
     4321 gtacactata cgggcatgct cgaagacggg aaaaaggttg actcaagtag agatcggaac
     4381 aagcctttca agttcatgtt ggggaaacaa gaggtaatac gcgggtggga ggagggagtc
     4441 gctcagatgt ctgtaggcca aagggcgaag ctcaccatat caccagatta cgcttacggg
     4501 gcgacgggcc atcccggaat aatacctccg cacgcgacac ttgtttttga tgttgaattg
     4561 cttaagttgg agaccagcag atctgtcgac cgctctagag gagtgcaggt ggaaaccatc
     4621 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc
     4681 gggatgcttg aagatggaaa gaaatttgac tcctcccggg acagaaacaa gccctttaag
     4741 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt
     4801 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac
     4861 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa
     4921 actagaggcg tgcaggtcga gaccatcagc cccggcgacg gccgcacctt tcccaagaga
     4981 ggccagactt gcgtggtcca ctacaccggc atgctggagg acggcaagaa gttcgacagc
     5041 agccgcgacc gcaacaagcc cttcaagttc atgctgggca aacaggaagt gatccgcggc
     5101 tgggaggaag gcgtggctca gatgagcgtg gggcagcggg ccaagctgac catcagcccc
     5161 gactatgcct acggcgccac cggccacccc ggcatcatcc ccccccacgc caccctggtg
     5221 ttcgacgtgg agctgctgaa gctggagact agttaggaat tcattgatca taatcagcca
     5281 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5341 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5401 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5461 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgta ccgagctcgc
     5521 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5581 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5641 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5701 ataatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5761 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5821 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5881 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5941 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6001 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6061 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6121 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6181 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6241 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6301 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6361 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6421 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6481 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6541 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6601 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6661 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6721 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6781 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6841 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6901 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6961 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7021 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7081 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7141 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7201 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7261 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7321 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7381 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7441 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7501 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7561 ttttcaataa gcggccgcgg ccatgccggc cactagtctc gagttattat tgaagcattt
     7621 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7681 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     7741 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     7801 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     7861 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7921 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgaaga
     7981 cgtcgcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
     8041 aaataaaaaa aattagtcag cgcgccattc gccattcagg ctgcgcaact gttgggaagg
     8101 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     8161 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     8221 tgccatctgc agtgaataat aaaatgtgtg tttgtccgaa atacgcgttt gagatttctg
     8281 tcccgactaa attcatgtcg cgcgatagtg gtgtttatcg ccgatagaga tggcgatatt
     8341 ggaaaaatcg atatttgaaa atatggcata ttgaaaatgt cgccgatgtg agtttctgtg
     8401 taactgatat cgccattttt ccaaaagttg atttttgggc atacgcgata tctggcgata
     8461 cgcttatatc gtttacgggg gatggcgata gacgcctttg gtgacttggg cgattctgtg
     8521 tgtcgcaaat
//
