LOCUS       sequence-209936-       10094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses vinculin tension sensor comprised of 
            Clover-(GGSGGS)5-mRuby2 module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   LaCroix AS, Lynch AD, Berginski ME, Hoffman BD
  TITLE     Tunable molecular tension sensors reveal extension-based control of 
            vinculin loading.
  JOURNAL   eLife. 2018 Jul 19;7. pii: 33927. doi: 10.7554/eLife.33927.
  PUBMED    30024378
REFERENCE   2  (bases 1 to 10094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-07-19"; volume: "7. pii: 33927. doi"; pages: " 
            10.7554/eLife.33927"
COMMENT     Sequence Label: VinTS-tCRMod-GGSGGS5
FEATURES             Location/Qualifiers
     source          1..10094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3623..3644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4231..4252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4352..5056
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="SKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRI
                     KVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVV
                     TVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHMA
                     LKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAK
                     FAGLGGGMDELYK"
     primer_bind     complement(5688..5705)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5694..5918
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5964..6392
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6051..6070)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6261..6282
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6401..6421)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6406..6735
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6586..6721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6648..6667
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6802..7596
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6856..6875)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7466..7485
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7770..7891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7807..7826)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7861..7880
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7940..7956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7940..7956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7953..7975)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7964..7980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7988..7994,7995..8012,8013..8018))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8033..8054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8171..8188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8342..8927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8422..8441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9098..9889,9890..9958))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9721..9740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9959..10063)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca tgcccgtctt ccacacgcgc accatcgaga gcatcttgga
      961 gcccgtggct cagcagatct cccacctggt catcatgcac gaggaggggg aggtagacgg
     1021 caaggccatc ccggacctca ccgcccccgt gtcggccgtg caggccgctg tcagcaacct
     1081 ggtgcgggtt ggaaaagaaa ctgtgcagac aacagaagac cagatcttga aaagggatat
     1141 gccaccagca ttcatcaaag tagagaatgc ctgcaccaag ctcgttcgag cagcccagat
     1201 gctgcaagca gatccttatt cagtaccagc tcgtgactac ctaattgatg gatcaagagg
     1261 catcctttct ggaacatcag acttacttct gacatttgat gaagcggagg tccgtaaaat
     1321 catccgtgtc tgcaaaggaa tattggaata tctgactgtg gcagaagtag tagagactat
     1381 ggaggatttg gtgacatata caaagaatct agggccagga atgacaaaga tggcgaaaat
     1441 gatcgatgag agacaacagg aattaactca tcaggaacat agggttatgc tggtgaactc
     1501 catgaatact gtgaaggagc tattgcctgt acttatttca gctatgaaga tctttgtaac
     1561 cacaaaaaac actaaaagcc agggaataga agaggccttg aaaaatcgca atttcacagt
     1621 agagaaaatg agtgctgaga taaatgaaat aatccgtgta ttacaactca cttcctggga
     1681 tgaagatgcc tgggccagca aggacactga agccatgaaa agagctttag ccctaataga
     1741 ttcaaagatg aaccaggcaa aaggctggct gagagatcca aacgcacctc caggggatgc
     1801 tggtgagcaa gcaataaggc agatccttga tgaagctgga aaagcaggag aattgtgtgc
     1861 aggcaaagaa cgcagggaga ttctgggaac atgcaaaact ttgggccaaa tgactgatca
     1921 acttgctgat ctccgagcta gaggacaggg tgctacaccg atggcaatgc agaaggcaca
     1981 gcaggtgtca caaggcctgg atttgctcac tgcaaaagtg gagaatgcag cccggaaatt
     2041 ggaggccatg acaaactcta agcaggctat tgcaaagaag attgatgctg ctcagaactg
     2101 gcttgcggat cccaacggcg gaagtgaagg agaagaacac attcgaggaa ttatgtctga
     2161 agcaaggaaa gttgcagaat tgtgtgagga gcctaaagaa agagatgata tccttcgctc
     2221 cttgggggaa atctctgctc tgacagctaa gctgtcagat ctgcgacgac atgggaaagg
     2281 cgactctcct gaggcccgtg cattggccaa gcaaatagct acatcacttc agaacttaca
     2341 gtccaaaaca aacagagctg tagcaaatac taggccagtt aaagctgctg tccatttgga
     2401 gggcaagatt gagcaagccc agaggtggat agataatcct acagttgatg atcggggagt
     2461 aggccaggca gcaattcggg gtttggttgc agaaggtcgt cgtctagcca atgtcatgat
     2521 gggaccttat cgtcaggacc tgcttgccaa atgtgaccgt gtagaccagc tggctgctca
     2581 gcttgctgac cttgcagcaa gaggggaagg agagtctcct caggctaggg caattgctgc
     2641 tcagcttcag gactccctga aggatctcaa agcacggatg caagaagcaa tgacccagga
     2701 ggtgtctgat gttttcagtg acactacaac ccctattaag ttgttagcag tagcagccac
     2761 tgctccttct gatactccca atagagaaga ggtgtttgaa gaaagagcag caaattttga
     2821 aaaccatgct gctagactgg gagcaacagc agaaaaagca gctgcagttg gaactgctaa
     2881 taaaactact gtggaaggca ttcaggcaac agtcaaatct gcaagggagc ttacaccaca
     2941 ggtagtatcg gctgctcgaa tcctcctgag aaatcctgga aatcaagctg cttatgagca
     3001 ttttgagaca atgaaaaacc aatggattga taatgtagaa aagatgacag ggctggtgga
     3061 tgaggccatc gataccaagt ctctgttgga tgcatcagaa gaggctatta agaaggatct
     3121 tgataaatgt aaagttgcaa tggccaatat gcaacctcag atgctggtag ctggagccac
     3181 cagcattgct agacgagcga accgcatcct gcttgtggca aaacgggagg ttgaaaattc
     3241 agaagaccct aaattcaggg aggctgttaa agcagcttct gatgagctga gcaaaaccat
     3301 atcaccgatg gtaatggatg ctaaagctgt agcaggaaat atctctgatc ctggtttgca
     3361 gaagagtttc ttggattctg gatacaggat tctgggagct gtggccaaag tcagagaagc
     3421 ctttcagcct caggagccag actttccccc tcctcctcct gacctagagc atctccatct
     3481 gactgatgag cttgctcctc caaaaccacc acttccagaa ggtgaggttc ccccacccag
     3541 accaccaccc cctgaagaaa aagatgaggt cgagatgatg gtgagcaagg gcgaggagct
     3601 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3661 cagcgtccgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     3721 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     3781 cgtggcctgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3841 catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa
     3901 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3961 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     4021 ccacaacgtc tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat
     4081 ccgccacaac gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4141 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgccct
     4201 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4261 cgggggtgga tccggtggat caggtggatc cggtggtagt ggtggatccg gaggttctgg
     4321 tggatccggt ggttcaggtg gatccggagg tagcaagggc gaagagctga tcaaggaaaa
     4381 tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg
     4441 tgaaggagaa ggcaatccgt acatgggaac tcaaaccatg aggatcaaag tcatcgaggg
     4501 aggacccctg ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac
     4561 ttttatcaag tacccgaaag gcattcctga tttctttaaa cagtcctttc ctgagggttt
     4621 tacttgggaa agagttacga gatacgaaga tggtggagtc gtcaccgtca tgcaggacac
     4681 cagccttgag gatggctgtc tcgtttacca cgtccaagtc agaggggtaa actttccctc
     4741 caatggtccc gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta
     4801 tccagcagat ggtggtctga ggggatacac tcatatggca ctgaaagttg atggtggtgg
     4861 ccatctgtct tgctctttcg taacaactta caggtcaaaa aagaccgtcg ggaacatcaa
     4921 gatgcccggt atccatgccg ttgatcaccg cctggaaagg ttagaggaaa gtgacaatga
     4981 aatgttcgta gtacaacgcg aacacgcagt tgccaagttc gccgggcttg gtggtgggat
     5041 ggacgagctg tacaaggcgg ccgcggagtt cccagagcag aaagcaggag aagctattaa
     5101 tcagcccatg atgatggctg ctaggcagtt gcatgacgag gctcggaaat ggtctagcaa
     5161 gggtaacgac atcattgctg ctgctaaacg aatggcgctg ctcatggctg agatgtcacg
     5221 cctggtgcga ggaggcagcg gaaacaagcg tgccctcatc cagtgtgcaa aagatattgc
     5281 taaggcatcg gatgaagtca ctcgattggc caaagaggtg gccaagcaat gtactgataa
     5341 acgcattaga acaaacctct tacaggtctg tgagcgaatc ccaaccatca gcacgcagct
     5401 caaaattctt tccacagtga aagctaccat gctgggcagg actaacatca gcgatgaaga
     5461 atcagaacag gcaactgaga tgttggttca taacgcccag aacctcatgc agtctgtgaa
     5521 ggaaactgtg agagaagctg aagcagcatc cattaagata agaacagatg ccggattcac
     5581 tctgcgctgg gtcagaaaga ccccatggta tcagtaagaa ttctgcagat atccagcaca
     5641 gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     5701 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5761 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5821 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5881 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5941 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6001 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6061 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6121 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6181 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6241 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6301 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6361 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     6421 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6481 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6541 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6601 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6661 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6721 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     6781 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     6841 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6901 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6961 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7021 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7081 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     7141 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     7201 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     7261 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     7321 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     7381 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     7441 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     7501 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     7561 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     7621 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     7681 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     7741 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     7801 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7861 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     7921 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7981 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8041 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8101 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8521 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8581 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8641 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8701 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8761 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8821 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     8881 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8941 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9001 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9061 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9121 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9181 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9241 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9301 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9361 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9421 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9481 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9541 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9601 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9661 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9721 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9781 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9841 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9901 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9961 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10021 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10081 tgccacctga cgtc
//
