LOCUS       sequence-209988-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 L128C/H113E
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgnanntagc agactgttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
       61 tgacctccat agaagacacc gactctagcc accatggact acaaagacga tgacgacaag
      121 tctagaatga tcgtggcgga ctccgagtgc cgcgcagagc tcaaggacta cctgcggttc
      181 gccccgggcg gcgtcggcga ctcgggcccc ggagaggagc agagggagag ccgggctcgg
      241 cgaggccctc gagggcccag cgccttcatc cccgtggagg aggtccttcg ggagggggct
      301 gagagcctcg agcagcacct ggggctggag gcactgatgt cctctgggcg agtagacaac
      361 ctggcagtgg tgatgggcct gcaccctgac tactttacca gcttctggcg cctgcactac
      421 ctgctgctgc acacggatgg tcccttggcc agctcctggc gcgagtacat tgccatcatg
      481 gctgccgccc gccatcagtg ttcttactgc gtaggctccc acatggccga gtttctgcag
      541 actggtggtg accctgagtg gctgctgggc ctccaccggg cccccgagaa gctgcgcaaa
      601 ctcagcgaga tcaacaagtt gctggcgcat cggccatggc tcatcaccaa ggaacacatc
      661 caggccttgc tgaagaccgg cgagcacact tggtccctgg ccgagctcat tcaggctctg
      721 gtcctgctca cccactgcca ctcgctctcc tccttcgtgt ttggctgtgg catcctccct
      781 gagggggatg cagatggcag ccctgccccc caggcaccta caccccctag tgaacagagc
      841 agccccccaa gcagggaccc gttgaacaac tctgggggct ttgagtctgc ccgcgacgtg
      901 gangcgctga tggagcgcat gcagcanctg
//
