LOCUS       sequence-209992-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 H132A
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgntgncnnt agcagagctg tttngtgaac cgtcagatcg cctggagacg ccatccacgc
       61 tgttttgacc tccatagaag acaccgactc tagccaccat ggactacaaa gacgatgacg
      121 acaagtctag aatgatcgtg gcggactccg agtgccgcgc agagctcaag gactacctgc
      181 ggttcgcccc gggcggcgtc ggcgactcgg gccccggaga ggagcagagg gagagccggg
      241 ctcggcgagg ccctcgaggg cccagcgcct tcatccccgt ggaggaggtc cttcgggagg
      301 gggctgagag cctcgagcag cacctggggc tggaggcact gatgtcctct gggcgagtag
      361 acaacctggc agtggtgatg ggcctgcacc ctgactactt taccagcttc tggcgcctgc
      421 actacctgct gctgcacacg gatggtccct tggccagctc ctggcgccac tacattgcca
      481 tcatggctgc cgcccgccat cagtgttctt acctggtagg ctccgccatg gccgagtttc
      541 tgcagactgg tggtgaccct gagtggctgc tgggcctcca ccgggccccc gagaagctgc
      601 gcaaactcag cgagatcaac aagttgctgg cgcatcggcc atggctcatc accaaggaac
      661 acatccaggc cttgctgaag accggcgagc acacttggtc cctggccgag ctcattcagg
      721 ctctggtcct gctcacccac tgccactcgc tctcctcctt cgtgtttggc tgtggcatcc
      781 tccctgaggg ggatgcagat ggcagccctg ccccccaggc acctacaccc cctagtgaac
      841 agagcagccc cccaagcagg gacccgttga acaactctgg gggctttgag tctgcccgcg
      901 acgtggaagc gctgatggan cgcatgcagc a
//
