LOCUS       sequence-209998-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 C399S
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..124
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgnanntag caganctgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgcct
       61 gttttgacct ccatagaaga caccgactct agccaccatg gactacaaag acgatgacga
      121 caagtctaga atgatcgtgg cggactccga gtgccgcgca gagctcaagg actacctgcg
      181 gttcgccccg ggcggcgtcg gcgactcggg ccccggagag gagcagaggg agagccgggc
      241 tcggcgaggc cctcgagggc ccagcgcctt catccccgtg gaggaggtcc ttcgggaggg
      301 ggctgagagc ctcgagcagc acctggggct ggaggcactg atgtcctctg ggcgagtaga
      361 caacctggca gtggtgatgg gcctgcaccc tgactacttt accagcttct ggcgcctgca
      421 ctacctgctg ctgcacacgg atggtccctt ggccagctcc tggcgccact acattgccat
      481 catggctgcc gcccgccatc agtgttctta cctggtaggc tcccacatgg ccgagtttct
      541 gcagactggt ggtgaccctg agtggctgct gggcctccac cgggcccccg agaagctgcg
      601 caaactcagc gagatcaaca agttgctggc gcatcggcca tggctcatca ccaaggaaca
      661 catccaggcc ttgctgaaga ccggcgagca cacttggtcc ctggccgagc tcattcaggc
      721 tctggtcctg ctcacccact gccactcgct ctcctccttc gtgtttggct gtggcatcct
      781 ccctgagggg gatgcagatg gcagccctgc cccccaggca cctacacccc ctagtgaaca
      841 gagcagcccc ccaagcaggg acccgttgaa caactctggg ggctttgagt ctgcccgcga
      901 cgtggangcg ctgatggagc gcatgcagca
//
