LOCUS       sequence-210012-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 R404A/D406A/D407A
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagnganctc gtttagtgac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgactc tagccaccat ggactacaaa gacgatgacg acaagtctag
      121 aatgatcgtg gcggactccg agtgccgcgc agagctcaag gactacctgc ggttcgcccc
      181 gggcggcgtc ggcgactcgg gccccggaga ggagcagagg gagagccggg ctcggcgagg
      241 ccctcgaggg cccagcgcct tcatccccgt ggaggaggtc cttcgggagg gggctgagag
      301 cctcgagcag cacctggggc tggaggcact gatgtcctct gggcgagtag acaacctggc
      361 agtggtgatg ggcctgcacc ctgactactt taccagcttc tggcgcctgc actacctgct
      421 gctgcacacg gatggtccct tggccagctc ctggcgccac tacattgcca tcatggctgc
      481 cgcccgccat cagtgttctt acctggtagg ctcccacatg gccgagtttc tgcagactgg
      541 tggtgaccct gagtggctgc tgggcctcca ccgggccccc gagaagctgc gcaaactcag
      601 cgagatcaac aagttgctgg cgcatcggcc atggctcatc accaaggaac acatccaggc
      661 cttgctgaag accggcgagc acacttggtc cctggccgag ctcattcagg ctctggtcct
      721 gctcacccac tgccactcgc tctcctcctt cgtgtttggc tgtggcatcc tccctgaggg
      781 ggatgcagat ggcagccctg ccccccaggc acctacaccc cctagtgaac agagcagccc
      841 cccaagcagg gacccgttga acaactctgg gggctttgag tctgcccgcg acgtggaggc
      901 gctgatggag cgcatgcagc agctgcag
//
