LOCUS       sequence-210014-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 D409A/G411A/Q415A
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gnnntanaan ctcgtttagt gaccgtcaga tcgcctggag acgccatcca cgctgttttg
       61 acctccatag aagacaccga ctctagccac catggactac aaagacgatg acgacaagtc
      121 tagaatgatc gtggcggact ccgagtgccg cgcagagctc aaggactacc tgcggttcgc
      181 cccgggcggc gtcggcgact cgggccccgg agaggagcag agggagagcc gggctcggcg
      241 aggccctcga gggcccagcg ccttcatccc cgtggaggag gtccttcggg agggggctga
      301 gagcctcgag cagcacctgg ggctggaggc actgatgtcc tctgggcgag tagacaacct
      361 ggcagtggtg atgggcctgc accctgacta ctttaccagc ttctggcgcc tgcactacct
      421 gctgctgcac acggatggtc ccttggccag ctcctggcgc cactacattg ccatcatggc
      481 tgccgcccgc catcagtgtt cttacctggt aggctcccac atggccgagt ttctgcagac
      541 tggtggtgac cctgagtggc tgctgggcct ccaccgggcc cccgagaagc tgcgcaaact
      601 cagcgagatc aacaagttgc tggcgcatcg gccatggctc atcaccaagg aacacatcca
      661 ggccttgctg aagaccggcg agcacacttg gtccctggcc gagctcattc aggctctggt
      721 cctgctcacc cactgccact cgctctcctc cttcgtgttt ggctgtggca tcctccctga
      781 gggggatgca gatggcagcc ctgcccccca ggcacctaca ccccctagtg aacagagcag
      841 ccccccaagc agggacccgt tgaacaactc tgggggcttt gagtctgccc gcgacgtgga
      901 ngcgctgatg gagcgcatgc agcanctgca g
//
