LOCUS       sequence-210016-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 R419A
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gnnnnntagn gagctcgttt agtgnaccgt cagatcgcct ggagacgcca tccacgctgt
       61 tttgacctcc atagaagaca ccgactctag ccaccatgga ctacaaagac gatgacgaca
      121 agtctagaat gatcgtggcg gactccgagt gccgcgcaga gctcaaggac tacctgcggt
      181 tcgccccggg cggcgtcggc gactcgggcc ccggagagga gcagagggag agccgggctc
      241 ggcgaggccc tcgagggccc agcgccttca tccccgtgga ggaggtcctt cgggaggggg
      301 ctgagagcct cgagcagcac ctggggctgg aggcactgat gtcctctggg cgagtagaca
      361 acctggcagt ggtgatgggc ctgcaccctg actactttac cagcttctgg cgcctgcact
      421 acctgctgct gcacacggat ggtcccttgg ccagctcctg gcgccactac attgccatca
      481 tggctgccgc ccgccatcag tgttcttacc tggtaggctc ccacatggcc gagtttctgc
      541 agactggtgg tgaccctgag tggctgctgg gcctccaccg ggcccccgag aagctgcgca
      601 aactcagcga gatcaacaag ttgctggcgc atcggccatg gctcatcacc aaggaacaca
      661 tccaggcctt gctgaagacc ggcgagcaca cttggtccct ggccgagctc attcaggctc
      721 tggtcctgct cacccactgc cactcgctct cctccttcgt gtttggctgt ggcatcctcc
      781 ctgaggggga tgcagatggc agccctgccc cccaggcacc tacaccccct agtgaacaga
      841 gcagcccccc aagcagggac ccgttgaaca actctggggg ctttgagtct gcccgcgacg
      901 tggaagcgct gatggagcgc atgcagcagc t
//
