LOCUS       sequence-210018-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 R419A/K422A/K426A
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagngactcg tttagtgacc gtcagatcgc ctggagacgc catccacgct gttttgacct
       61 ccatagaaga caccgactct agccaccatg gactacaaag acgatgacga caagtctaga
      121 atgatcgtgg cggactccga gtgccgcgca gagctcaagg actacctgcg gttcgccccg
      181 ggcggcgtcg gcgactcggg ccccggagag gagcagaggg agagccgggc tcggcgaggc
      241 cctcgagggc ccagcgcctt catccccgtg gaggaggtcc ttcgggaggg ggctgagagc
      301 ctcgagcagc acctggggct ggaggcactg atgtcctctg ggcgagtaga caacctggca
      361 gtggtgatgg gcctgcaccc tgactacttt accagcttct ggcgcctgca ctacctgctg
      421 ctgcacacgg atggtccctt ggccagctcc tggcgccact acattgccat catggctgcc
      481 gcccgccatc agtgttctta cctggtaggc tcccacatgg ccgagtttct gcagactggt
      541 ggtgaccctg agtggctgct gggcctccac cgggcccccg agaagctgcg caaactcagc
      601 gagatcaaca agttgctggc gcatcggcca tggctcatca ccaaggaaca catccaggcc
      661 ttgctgaaga ccggcgagca cacttggtcc ctggccgagc tcattcaggc tctggtcctg
      721 ctcacccact gccactcgct ctcctccttc gtgtttggct gtggcatcct ccctgagggg
      781 gatgcagatg gcagccctgc cccccaggca cctacacccc ctagtgaaca gagcagcccc
      841 ccaagcaggg acccgttgaa caactctggg ggctttgagt ctgcccgcga cgtggaagcg
      901 ctgatggagc gcatgcagca actgcagg
//
