LOCUS       sequence-210020-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 D406A
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gnnntagnga ctcgtttagt gaccgtcaag atcgcctgga gacgccatcc acgctgtttt
       61 gacctccata gaagacaccg actctagcca ccatggacta caaagacgat gacgacaagt
      121 ctagaatgat cgtggcggac tccgagtgcc gcgcagagct caaggactac ctgcggttcg
      181 ccccgggcgg cgtcggcgac tcgggccccg gagaggagca gagggagagc cgggctcggc
      241 gaggccctcg agggcccagc gccttcatcc ccgtggagga ggtccttcgg gagggggctg
      301 agagcctcga gcagcacctg gggctggagg cactgatgtc ctctgggcga gtagacaacc
      361 tggcagtggt gatgggcctg caccctgact actttaccag cttctggcgc ctgcactacc
      421 tgctgctgca cacggatggt cccttggcca gctcctggcg ccactacatt gccatcatgg
      481 ctgccgcccg ccatcagtgt tcttacctgg taggctccca catggccgag tttctgcaga
      541 ctggtggtga ccctgagtgg ctgctgggcc tccaccgggc ccccgagaag ctgcgcaaac
      601 tcagcgagat caacaagttg ctggcgcatc ggccatggct catcaccaag gaacacatcc
      661 aggccttgct gaagaccggc gagcacactt ggtccctggc cgagctcatt caggctctgg
      721 tcctgctcac ccactgccac tcgctctcct ccttcgtgtt tggctgtggc atcctccctg
      781 agggggatgc agatggcagc cctgcccccc aggcacctac accccctagt gaacagagca
      841 gccccccaag cagggacccg ttgaacaact ctgggggctt tgagtctgcc cgcgacgtgg
      901 aagcgctgat ggagcgcatg cagcagctgc a
//
