LOCUS       sequence-210022-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 D407A
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gnanntagca gactgttagt gaaccgtcag atcgcctgga agacgccatc cacgcctgtt
       61 ttgacctcca tagaagacac cgactctagc caccatggac tacaaagacg atgacgacaa
      121 gtctagaatg atcgtggcgg actccgagtg ccgcgcagag ctcaaggact acctgcggtt
      181 cgccccgggc ggcgtcggcg actcgggccc cggagaggag cagagggaga gccgggctcg
      241 gcgaggccct cgagggccca gcgccttcat ccccgtggag gaggtccttc gggagggggc
      301 tgagagcctc gagcagcacc tggggctgga ggcactgatg tcctctgggc gagtagacaa
      361 cctggcagtg gtgatgggcc tgcaccctga ctactttacc agcttctggc gcctgcacta
      421 cctgctgctg cacacggatg gtcccttggc cagctcctgg cgccactaca ttgccatcat
      481 ggctgccgcc cgccatcagt gttcttacct ggtaggctcc cacatggccg agtttctgca
      541 gactggtggt gaccctgagt ggctgctggg cctccaccgg gcccccgaga agctgcgcaa
      601 actcagcgag atcaacaagt tgctggcgca tcggccatgg ctcatcacca aggaacacat
      661 ccaggccttg ctgaagaccg gcgagcacac ttggtccctg gccgagctca ttcaggctct
      721 ggtcctgctc acccactgcc actcgctctc ctccttcgtg tttggctgtg gcatcctccc
      781 tgagggggat gcagatggca gccctgcccc ccaggcacct acacccccta gtgaacagan
      841 cagcccccca agcanggaac ccgttgaaca actctngggg gctttnagtc tgcccncgac
      901 gtgnangcnc tnatggancg catgcancan c
//
