LOCUS       sequence-210025-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of mSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Ro SH, Semple IA, Park H, Park H, Park HW, Kim M, Kim JS, Lee JH
  TITLE     Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of 
            p62/sequestosome-1.
  JOURNAL   FEBS J. 2014 Sep;281(17):3816-27. doi: 10.1111/febs.12905. Epub 2014
            Jul 28.
  PUBMED    25040165
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/febs.12905"; journalName: "FEBS J"; date: "2014-09"; 
            volume: "281"; issue: "17"; pages: "3816-27"
COMMENT     Sequence Label: pcDNA-Flag-Sesn-A mSesn2 (delBC)
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             118..141
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(823..841)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(824..841)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(861..878)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggnctntagc agactctctg gctactagag aacccactgc ttactggctt atcgaaatta
       61 atacgactca ctatagggag acccaagctt ggtaccgagc tcggatccac cgccatggac
      121 tacaaggacg atgatgacaa gggcgaattc atgatcgtag cggactccga gtgccattcc
      181 gagatcaagg gctacctgcc gttcaccagg ggtggtgtgg ccggccccga gacccgagag
      241 gagcatcggg agggccaggc tcggcgaggc tctcgagggc ccagcgcttt cattccagtg
      301 gaagagatcc ttcgggaagg agctgagagc ctggagcagc acctggggct cgaggccctg
      361 atgtcctcag ggcgggtgga caacctggcg gtggtgatgg gtctacaccc ggactacctt
      421 agcagcttct ggcgcctgca ttacctgctg ctgcatacgg atgggcccct ggccagctcc
      481 tggcgccact acattgccat catggctgct gcccgtcacc agtgttctta cctggtgggt
      541 tcccacatga ctgagttcct gcagaccggc ggtgaccccg agtggctgct gggccttcac
      601 cgggcccctg agaagctccg caagctcagc gaggtcaaca agttactggc gcatcggccg
      661 tggctcatca ccaaggagca tatccaggcc ttgctgaaga ctggcgagca cagctggtcc
      721 ctggccgaac tcatccaagc actggtcctg cttacccact gccactctct ggcctccttt
      781 gtgtttggct gttgagtcga gcatgcatct agagggccct attctatagt gtcacctaaa
      841 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      901 cccctccccc gtgccttcct tgaccctgg
//
