LOCUS       sequence-210027-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of mSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ro SH, Semple IA, Park H, Park H, Park HW, Kim M, Kim JS, Lee JH
  TITLE     Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of 
            p62/sequestosome-1.
  JOURNAL   FEBS J. 2014 Sep;281(17):3816-27. doi: 10.1111/febs.12905. Epub 2014
            Jul 28.
  PUBMED    25040165
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/febs.12905"; journalName: "FEBS J"; date: "2014-09"; 
            volume: "281"; issue: "17"; pages: "3816-27"
COMMENT     Sequence Label: pcDNA-Flag-Sesn-C mSesn2 (delAB)
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             120..143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(693..711)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(694..711)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(731..748)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    737..961
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgtgggntta gngactctct ggctactaga gaaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc accgccatgg
      121 actacaagga cgatgatgac aagggcgaat tcgacatcct atgctttgtg gaggaccccg
      181 catttggata tgaagacttc actcgtcgag ggactcaggc gcccccaacc ttccgtgccc
      241 aggattatac ctgggaagac catggctact cgctgatcca gaggctctac cctgagggag
      301 ggcagctgct ggatgagaag ttccaggtag cctgcagcct cacctataac accatcgcca
      361 tgcacagtgg cgtggacacc tccatgctcc gcagggccat ctggaactac atccactgcg
      421 tctttggcat cagatacgat gactatgatt acggcgaggt aaaccagctc ctggaacgga
      481 acctcaaaat ctatatcaag acggtggcct gctaccctga gaagacgacc cgtaggatgt
      541 acaacctctt ttggaggcac ttccgccact cagagaaggt tcatgtgaac ttgctgctcc
      601 ttgaagcccg catgcaagcg gccctgctct atgccctgcg cgccatcacc cggtacatga
      661 cctgagtcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
      721 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      781 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      841 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      901 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      961 gcttctgagg cggaaagaac cagctggggc tctaggggta tccccacgcg ccctgtagcg
     1021 ncgcataagc gcggcgggtg
//
