LOCUS       sequence-210034-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of hATG5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kim M, Sandford E, Gatica D, Qiu Y, Liu X, Zheng Y, Schulman BA, Xu 
            J, Semple I, Ro SH, Kim B, Mavioglu RN, Tolun A, Jipa A, Takats S, 
            Karpati M, Li JZ, Yapici Z, Juhasz G, Lee JH, Klionsky DJ, 
            Burmeister M
  TITLE     Mutation in ATG5 reduces autophagy and leads to ataxia with 
            developmental delay
  JOURNAL   Elife. 2016 Jan 26;5. pii: e12245. doi: 10.7554/eLife.12245.
  PUBMED    26812546
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-01-26"; volume: "5. pii: e12245. doi"; pages: " 
            10.7554/eLife.12245"
COMMENT     Sequence Label: pLU-CMV-Flag-hATG5 WT
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggcntancga ctcgtttagt gaccgtcaga tcgcctggag acgccatcca cgctgttttg
       61 acctccatag aagacaccga ctctagccac catggactac aaagacgatg acgacaagtc
      121 tagaatgaca gatgacaaag atgtgcttcg agatgtgtgg tttggacgaa ttccaacttg
      181 tttcacgcta tatcaggatg agataactga aagggaagca gaaccatact atttgctttt
      241 gccaagagta agttatttga cgttggtaac tgacaaagtg aaaaagcact ttcagaaggt
      301 tatgagacaa gaagacatta gtgagatatg gtttgaatat gaaggcacac cactgaaatg
      361 gcattatcca attggtttgc tatttgatct tcttgcatca agttcagctc ttccttggaa
      421 catcacagta cattttaaga gttttccaga aaaagacctt ctgcactgtc catctaagga
      481 tgcaattgaa gctcatttta tgtcatgtat gaaagaagct gatgctttaa aacataaaag
      541 tcaagtaatc aatgaaatgc agaaaaaaga tcacaagcaa ctctggatgg gattgcaaaa
      601 tgacagattt gaccagtttt gggccatcaa tcggaaactc atggaatatc ctgcagaaga
      661 aaatggattt cgttatatcc cctttagaat atatcagaca acgactgaaa gacctttcat
      721 tcagaagctg tttcgtcctg tggctgcaga tggacagttg cacacactag gagatctcct
      781 caaagaagtt tgtccttctg ctattgatcc tgaagatggg gaaaaaaaga atcaagtgat
      841 gattcatgga attgagccaa tgttggaaac acctctgcag tggctgagtg aacatctgag
      901 ctacccggat aattttcttc atattagtat c
//
