LOCUS       sequence-210065-        3711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Preparation of RNA probe for ISH of mouse Vmn2r120 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Akiyoshi S, Ishii T, Bai Z, Mombaerts P
  TITLE     Subpopulations of vomeronasal sensory neurons with coordinated 
            coexpression of type 2 vomeronasal receptor genes are differentially
            dependent on Vmn2r1.
  JOURNAL   Eur J Neurosci. 2018 Apr;47(7):887-900. doi: 10.1111/ejn.13875. Epub
            2018 Mar 25.
  PUBMED    29465786
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/ejn.13875";
            journalName: "Eur J Neurosci"; date: "2018-04"; volume: "47"; issue:
            "7"; pages: "887-900"
COMMENT     Sequence Label: pISH-Vmn2r120-TR
FEATURES             Location/Qualifiers
     source          1..3711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(836..854)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(837..854)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(872..888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(872..888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(885..907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    896..912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(920..926,927..944,945..950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    965..986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1103..1120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1274..1862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1354..1373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2033..2824,2825..2893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2656..2675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2894..2998)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3076..3531)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3213..3234)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3425..3444
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3657..3679
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3671..3688
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3672..3688
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3695..3711,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3695..3711,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tagggatgca gtgagtttgt tctgtgttgg catactgatc atctggacac tttatacact
      121 gaatactgtc tgtctcattg gagatttcat tctctaagca ggggtggcaa tcaaaacagc
      181 atgaagcctt gccctcctga ggggatttca ttaatccagg agcacagctt gtactgcaca
      241 cagacagggg agtctgtttg tctttggtgc cccactcgat catgccatct gacagagaga
      301 actgtttgtt acgtggaaga aaagaagaaa atgtccctac tttcacctta agaccaagac
      361 cttgtgggaa gttccagaag ttaaaaatgt catactttgt ttccagtttt cctgtatggc
      421 tcagatctac tttgtctcca ctaggattat taaactggat gttcttcagg aaagggtgta
      481 gcctacaagg ggaaaaaacc agtcttttcc tgcttttcac tggttgtaca tctgcatgtt
      541 cttggagcat ttcatggagg ctgtgggcaa ctgcatacac tgcgttatat atgtgataac
      601 tcccttcact catgtccatg tcaaatcggt gccaaggtaa caatgccaag gatccattgg
      661 atgaacaagt tttcagtgtt ttacagtaaa cccgtgaaaa tggacaattc aaatacattt
      721 tccatatatg tgaaagaaag atatcatctg gatataatca ctagtgaatt cgcggccgcc
      781 tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat
      841 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      901 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      961 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1021 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1681 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2341 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3001 catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3061 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3121 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3181 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3241 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3301 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3361 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3421 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3481 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     3541 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg tcctcttcgc tattacgcca
     3601 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     3661 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat a
//
