LOCUS       sequence-210076-        6528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Codon-optimized Archon1 fluorescent voltage reporter in zebrafish 
            expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum
            DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades
            JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C,
            Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, 
            Sabatini BL, Boyden ES
  TITLE     A robotic multidimensional directed evolution approach applied to 
            fluorescent voltage reporters.
  JOURNAL   Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9.
  PUBMED    29483642
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9."
COMMENT     Sequence Label: pTol2-UAS-zArchon1-KGC-EGFP-ER2
FEATURES             Location/Qualifiers
     source          1..6528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(9..20,21..183)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    699..723
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(951..975)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1856..1858,1859..2569)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    3899..3980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3936..3955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3990..4009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(4370..4557,4558..4569)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4595..4612)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4766..5354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4846..4865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5525..6316,6317..6385))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6148..6167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6386..6490)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact
       61 tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa
      121 agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa
      181 gaaagaaaac tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc
      241 atacaaggtt ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga
      301 caatctaatg ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga
      361 agctgattct caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct
      421 gcagtgctga aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata
      481 gagaggctgc aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc
      541 agatgggcca ttcgtacgct taatttcgaa tatcgatatc aagggcccat ctcgagtatc
      601 cggacaccta ggtagcatgc atggcgcctt gtcgactagc tagcatcccg ggaagaattc
      661 ctcgacggat ccaccggtgt ggaattctgc agatatcaac aagtttgtac aaaaaagcag
      721 gctggccatc aagcttaggc ctccacggtg gcttctaatc cgtgagtcct agcgggtgac
      781 agccctccgt cttcacaggc ggaggagagt cttccgtagg gttcctcgga gtactgtcct
      841 ccgacgcgtg caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct
      901 cactttgagc tcctccacac gaattccctc gacctcgaag acgcgtggat acccagcttt
      961 cttgtacaaa gtggtcacta gtgccaccat ggtgagcatc gctctgcagg ctggatacga
     1021 cctgctggga gacggaagac ctgagagcct gtggctggga atcggaacac tgctgatgct
     1081 gatcggaaca ttctacttcc tggtgagagc ttggggagag acagacaagg acgctagaga
     1141 gtactacgct gtgacaatcc tggtgagcgg aatcgctagc gctgcttacc tgagcatgtt
     1201 cttcggaatc ggactgacag aggtgcctgt gggaggagag atgctgaaca tctactacgc
     1261 tagatacgct cagtggctgt tcacaacacc tctgctgctg ctgcacctgg ctctgctggc
     1321 taaggtggac agagtgacaa tcggaacact ggtgggagtg gacgctctga tgatcgtgac
     1381 aggactgatc ggaacactga gccacacagc tatcgctaga tacagctggt ggctgttcag
     1441 cacaatctgt atgatcgtgg tgctgtacgt gctggctaca agcctgagaa gcgctgctaa
     1501 ggagagagga cctgaggtgg ctagcacatt caacatcctg acagctctgg tgctggtgct
     1561 gtggacagct taccctatca tctggatcat cggaacagag ggagctggag tggtgggact
     1621 gggaatcgag acactgctgt tcatggtgct ggacgtgaca gctaaggtgg gattcggatt
     1681 catcctgctg agaagcagag ctatcctgca ggacacagag gctcctgagc ctagcgctgg
     1741 agctgacaga tctgctgctg acagacctgt ggtggctgtg agcaaggctg ctgctaagag
     1801 cagaatcaca agcgagggag agtacatccc tctggaccag atcgacatca acgtggtgag
     1861 caagggagag gagctgttca caggagtggt gcctatcctg gtggagctgg acggagacgt
     1921 gaacggacac aagttcagcg tgagcggaga gggagaggga gacgctacat acggaaagct
     1981 gacactgaag ttcatctgta caacaggaaa gctgcctgtg ccttggccta cactggtgac
     2041 aacactgaca tacggagtgc agtgtttcag cagataccct gaccacatga agcagcacga
     2101 cttcttcaag agcgctatgc ctgagggata cgtgcaggag agaacaatct tcttcaagga
     2161 cgacggaaac tacaagacaa gagctgaggt gaagttcgag ggagacacac tggtgaacag
     2221 aatcgagctg aagggaatcg acttcaagga ggacggaaac atcctgggac acaagctgga
     2281 gtacaactac aacagccaca acgtgtacat catggctgac aagcagaaga acggaatcaa
     2341 ggtgaacttc aagatcagac acaacatcga ggacggaagc gtgcagctgg ctgaccacta
     2401 ccagcagaac acacctatcg gagacggacc tgtgctgctg cctgacaacc actacctgag
     2461 cacacagagc gctctgagca aggaccctaa cgagaagaga gaccacatgg tgctgctgga
     2521 gttcgtgaca gctgctggaa tcacactggg aatggacgag ctgtacaagt tctgttacga
     2581 gaacgaggtg tgaggcgcgc caacccgctc ctcctccctc cctccctccc tccctcctct
     2641 cctttattca cttttcaaca tgcacaacac gtcatcaaat cccccaatca gcactgaatt
     2701 acagccacgg gccaccagac tgaaggtgtt aaagaagacg attcggtctc atcagcggct
     2761 aaaacgagat gagggtgaac cgagagagac tgtttccaca gatgttcttg aaggagggcc
     2821 gggctcgtag gtgcggttat tacacatata agctcccatt cacaccttaa aacatccaac
     2881 agcattccaa agttttccat cggggaccag ggatagaaaa cactgcctct tccactgcct
     2941 aatttaacac attccttcta gattatctct ggtcatctag atgaaatcag ttctgttctg
     3001 tttgccgtgt ctgaaaagac agtaaagagt aattgaaaaa aaaaatgaat atagaaaaat
     3061 ggggactgga gagatatttg gactagacgc tcctgatcca gccagaagga acctttcagg
     3121 tgtcgattac actgcctgcg aaaacaacct cttcttccat tcttcagttt ccagctgccg
     3181 gctggcttct tccactgccc aacatcctaa caaacccctg acaaaaaagg agacaagcct
     3241 tgaaacatcc tgaaataagc aattcttagc tgccagggac gtcttcctgt tagcatcagt
     3301 gtatttgttt cttttgcttt gatttgtttt ctttttttat tttattttca tggtaaatgt
     3361 attctgcctg agcactttaa acgaaggtgt ttttgttcca gtcgatgctt cgttttgaac
     3421 ctgcttttat atttattaac ttttgttgtg tatttttaat gtttaatctt taaaggaata
     3481 gttcacctac acacacacac acacacacac acacacacac aaatgaaatg aagtcatcat
     3541 ttactcactt ttctttcttt tagctgtgga gcacaaaacg agatgttttg aagaatgttc
     3601 acgctgttgt ttttcatgca atttaagtaa acgaagatca agggatgttg agctatacaa
     3661 atattcatta tatgggcagc acggtcgctg gttcgagtcc cggctgggcc agttggcatt
     3721 tctgagtttg catattctcc ctgtgttggc atgggtttcc tcctggtgct ccgtttctcc
     3781 cacagtccac tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3841 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatcagg
     4021 ccttagggat aacagggtaa tacgcgtgat ctgcgaagat acggccacgg gtgctcttga
     4081 tcctgtggct gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc
     4141 attttcttcc actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca
     4201 ttgtggtaat agatgtgaca tgacgtcact tccaaaggac caatgaacat gtctgaccaa
     4261 tttcatataa tgtgaaaacg attttcatag gcagaataaa taacatttaa attaaactgg
     4321 gcatcagcgc aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc
     4381 aaaaaataag tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt
     4441 ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag
     4501 taaaaatccc caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt
     4561 acacctctgg ttcttgaggt acctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4621 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4681 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4741 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4801 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4861 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4921 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4981 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5041 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5101 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5161 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5221 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5281 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5341 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5401 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5461 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5521 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5581 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5641 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5701 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5761 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5821 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5881 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5941 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6001 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6061 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6121 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6181 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6241 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6301 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6361 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6421 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6481 gggttccgcg cacatttccc cgaggtaccg atcggtatct gtccagga
//
